Objective: To investigate the relationship of the terminal position of the muscular contraction path and centric relation position with different vertical dimension.Methods: The terminal position of the muscular contraction path and centric relation position with different vertical dimension were recorded for ten edentulous patients with gothic arch.Results: The mesial distance between the terminal position of the muscular contraction path and centric relation position increased with the vertical dimension.The lateral dimension between the terminal position of the muscular contraction path and centric relation position,the distribution of the terminal position of the muscular contraction path are not correlated to the vertical dimension.The center of the terminal position of the muscular contraction path is 1-4 mm ahead of the centric relation position and(0.42?0.47) mm lateral to the centric relation position.The distribution of the terminal position of the muscular contraction path is(1.36?0.58) mm in the mesial-distal direction,(1.56?0.62) mm in the lateral direction.Conclusion: With the increase of the vertical dimension,the mesial distance between the terminal position of the muscular contraction path and centric relation position increased.

Objective To investigate the morphological characteristics of vertebral-basilar artery in patients with posterior circulation ischemia vertigo. Methods The data of cerebrovascular disease related risk factors,neck CT angiography,and head MR angiography of 122 patients with posterior circulation ischemia vertigo and 60 non-vertigo volunteers with cerebrovascular disease related risk factors over the same period were analyzed retrospectively. The positive index of single factor comparison was analyzed by using multivariate logistic regression analysis. The risk factors for posterior circulation ischemia vertigo were screened,and the degree of vertebral artery stenosis and the degree of basilar artery tortuosity of the posterior circulation ischemia group were compared with those of the control group. Results (1 )The incidences of hypertension,hyperlipidemia,previous history of stroke or transient ischemic attack,vertebral artery atherosclemtic stenosis,vertebral artery variation,and basilar artery tortuosity of the posterior circulation ischemia group were 59%(n=72),55. 7%(n=68),64. 8%(n=79),41. 0%(n=50),28. 7%(n=35),and 30. 3%(n=37),respectively,and they were all significant higher than 33. 3%(n=20),31. 7%(n=19),35. 0%(n=21),15. 0%(n=9),10. 0%(n=6),and 15. 0%(n=9)of the control group. There were significant differences (all P<0. 05). (2)Vertebral artery atherosclerotic stenosis (OR,3. 891, 95%CI 1. 721-8. 800,P <0. 01),vertebral artery variation (OR,3. 231,95%CI 1. 238-8. 432,P =0.017),and basilar artery tortuosity (OR,2. 664,95%CI 1. 189-5. 972,P=0. 017)were the independent risk factors for posterior circulation ischemic vertigo. The degrees of vertebral artery stenosis and basilar artery tortuosity of the posterior circulation ischemic group were higher than those of the control group. There were significant differences (all P<0. 05). There was significant difference in the vertebral artery dominance between the degree of basilar artery tortuosity ≥grade 2 and

BACKGROUND:Studies have shown that mesenchymsi stem cells (MSCs) participate in tumor stroma remodeling,but the mechanism is not clear.However,myofibroblast is related with tumor strema remodeling.OBJECTIVE:To investigate whether MSCs can differentiate into myofibroblast under the induction of tumor microenvironment.DESIGN,TIME AND SETING:This in vitro observation and in vivo transplantation cytology experiment was performed at institute of Low Temperature Medicine,Qilu Hospital of Shandong University from June 2006 to December 2007.MATERIALS:Twelve male New Zealand white rabbits,with 3-month-old,were purchased from Shandong Academy of Agricultural Sciences.METHODS:MSCs were obtained through bone marrow aspirates in male New Zealand rabbits and cultured by density gradient method.The 2~(nd) passage of MSCs were labeled with DAPI,and adjusted cell density to 5×10~(10) /L.The cells were assigned into experimental and control groups.For the experimental group,the passage two MSCs were cultured in low-glucose DMEM containing 10% fetal bovine serum and 30% VX2-cultured supernatant.For the control group,passage two MSCs were cultured in low-glucose DMEM containing 10% fetal bovine serum.Also,DAPI labeled self passage 2 MSCs of avery animal were delivered uniformly via direct surgical intratumoral injection to observe the differentiation of MSCs in tumor tissue.MAIN OUTCOME MEASURES:Immunofluorescense and RT-PCR were used to test the expression of α-SMA and Vimentin in MSCs of both groups 14 days later.At 3 weeks after VX2.intratumoral injection,Immunofluorescence was used to observe the distribution and differentiation of MSCs in tumor tissue.RESULTS:After stimulated with 30% VX2-cultured supematant for 2 weeks,Cy3 immunofluorescance showed that the expressions of α-SMA and Vimentin were strongly expressed in the experimental group,which was weakly expressed in the control group.Compared to the control group,the levels of α-SMA and Vimentin mRNA were obviously increased in the experimental group (P ＜ 0.01,P ＜ 0.05).At 3 weeks after MSCs intratumoral injection,these MSCs mainly distributed in tumor stroma and the expression of α-SMA and Vimentin in MSCs were also obviously increased,which demonstrated that the MSCs had differentiated into myofibroblasts.CONCLUSIONS:Under the induction of tumor microenivironment,MSCs can differentiate into myofibroblast,accordingly,accelerate the growth of tumor.

Objective To study the construction of tissue engineering adipose in vitro with silk fibroin scaffold and human umbilical cord mesenchymal stem cells (hUCMSCs) transfected by recombinant human insulin gene lentivirus.Methods hUCMSCs infected with recombinant lentiviral pLenti6.3-insulin-IRES-EGFP (Group A) by the best MOI=10 were seeded in silk fibroin scaffold; hUCMSCs transfected by EGFP gene (Group B)was regarded as negative control; and then the cell-scaf-fold complexes were cultured in adipogenic differentiation medium. MTT test was used to detect whether recombinant lentiviral affected the growth and proliferation of hUCMSCs and the growth activity of hUCMSCs seeded in silk fibroin.Results After 5-7 days for adipogenic culture,the numbers of fat-like cells in group A were significantly more than those in group B (P =0.007,P＜0.01).RTPCR results showed that the expression of PPARγ-2 in group A was much stronger than that in group B.MTT test showed that there was no significant difference in optical density (A)at each time between transfected group and nontransfected group (P=0.056,P＞0.05).And there was also no significant difference in optical density (A)between cell group and cell-scaffold group (P =0.066,P＞0.05).Conclusions Insulin gene could obviously promote hUCMSCs getting into adipose,and carrying recombinant human insulin gene lentiviral vector transfection of hUCMSCs and silk fibroin scaffolds could effectively construct tissue engineering adipose in vitro.

Objective To understand the prevalence of disinfectant-resistant gene qacA/B in the clinical strains of methicillin-resistant Staphylococcus aureus (MRSA) .Methods A total of 123 clinical consecutive MRSA strains were collected for PCR assay to detect qacA/B gene and mecA gene .Results The qacA/B gene was identified in 19 (15 .4% ) of the 123 MRSA strains . The mecA gene was positive in 89 .4% (110/123) of the MRSA strains .Conclusions The disinfectant-resistant gene qacA/B is prevalent in the hospital-acquired MRSA strains .Monitoring measures should be strengthened for appropriate selection of disinfectants in clinical practice .

Objective:To compare the healing of gingiva after electrosurgical gingivectomy with different power outputs and electrodes. Methods:15 rabbits were randomly assigned to 3 groups which used different electrodes: needle electrode, long loop electrode and loop electrode. 1 animal was chosen as control in each group. Free gingiva of anterior teeth had been removed 2 mm in height with different power output and electrosurgical knife. Gingival specimens were harvested for histopathological study on days 1,7,14,and 21 after operation. Statistical analysis was performed on wound healing. Results:Electrical knife with medium power output caused significantly higher degree of inflammation than other techniques(P0.05). Conclusion:When electrosurgery is used on gingiva, there is no significantly difference with different electrodes. Longer observation is needed after gingival electrosurgery with medium power output electrical knife.

Objective To evaluate the effects of different doses of propofol infusion on real-time monitoring of recurrent laryngeal nerve. Method 30 ASA Ⅰ-Ⅱ patients who underwent thyroid gland surgery were given propofol intravenously in different concentrations. BP, HR and real-time monitoring of recurrent laryngeal nerve were recorded with the propofol dosage varying from 3 to 6, 9, 12, 15, 18?g/(kg?min). Results The amplitude of recurrent laryngeal nerve reflex had no change with different doses of propofol infusion. The HR and BP were elevated with low doses of propofol infusion, but there were no changes when propofol concentration was 15 or 18?g/(kg?min). Conclusion Propofol infusion i.v. does not interfere with real-time intraoperative monitoring of recurrent laryngeal nerve, and it does not inhibit the action potential of recurrent laryngeal nerve, but can effectively decrease the cardiovescular reaction.

140?V)when the end tidal concentration of sevoflurane increased during the operation.The BIS were between 43-61 when the end tidal concentration of sevuflnrane was lower than 1.0 MAC.The BP and HR in the group decreased(P

Objective To study the effect of finasteride on bleeding associated with suprapubic prostatectomy and transurethral resection of prostate (TURP). Methods CD 34 ,bcl-2,PCNA and VEGF expressions in the prostatic tissues were studied by means of immunohistochemical method in 89 patients undergone prostatectomy,43 of them had taken fenateride and the other 46 did not.Acomparative study of clinical data and immunohistochemical parameters was made between the 2 groups. Results The volume of bleeding and blood transfusion needed were much less in the group treated with fenasteride.The microyascular density as studied by immunohistochemistry was much less in the fenasteride group.The expressions of bcl-2,PCNA and VEGF were also different between the 2 groups ( P

Objective To present a method for breast reconstruction with the deep inferior epigastric perforator (DIEP) flap and to summarize the operative experiences. Methods DIEP flaps were elevated without carrying rectus abdominis muscle. The breast was shaped after the deep inferior epigastric artery and vein were anastomosed to the internal mammary artery and vein. Results Since 2000, we have used the DIEP flaps in 15 cases of breast reconstruction. There were 100 % survival in 10 flaps, distal skin necrosis in 2 flaps, and a full loss in 2 flaps. With the follow-up of 6～12 months, the reconstructed breasts were well-shaped and there were no complications such as abdominal heria, bulge and weakness in donor sites. Conclusion DIEP flap is the development and refinement of TRAM flap and possesses of advantages such as rich blood supply, abundant tissue volume, minimal donor site morbidity and easy shaping. It is a new but reliable and safe technique for autologous breast reconstruction.