AIM: To study the chemical constituents from the stem bark of Aegiceras Corniculatum. METHODS: The compounds were extracted by alcohol and isolated by silica gel column chromatography,and their structures were elucidated by means of spectral analysis. RESULTS: Six compounds were identified as ?-sitosterol(Ⅰ),protocatechuic acid(Ⅱ), Vanillic acid(Ⅲ),daucosterol(Ⅳ),schimpefinone(Ⅴ),Primulagenin A(Ⅵ). CONCLUSION: All the compounds were isolated from this plant and reported for the first time.

Objective To analyze the distribution ,antibiotic resistance of the pathogens isolated from patients with stroke‐associated pneumonia (SAP) in Department of Respiratory Medicine for better clinical medication .Methods The SAP patients who were treated in the hospital from January 2007 to January 2014 were included in this study .The pathogens were cultured and isolated .Antimicrobial susceptibility of these pathogens was analyzed retrospectively .The multidrug resistant bacteria were identified .Pathogen distribution was compared between the pneumonia associated with cerebral hemorrhage and that associated with cerebral infarction .Results A total of 50 strains of bacterial pathogens were isolated from 40 (12 .3% ) of all the 325 SAP patients ,including 46 strains of gram negative bacilli (92 .0% ) (mainly P . aeruginosa ,15 ;E . coli ,11 ;A . baumannii ,5 ;and S .marcescens ,5) and 4 (8 .0% ) strains of S .aureus ,all resistant to methicillin (MRSA) .P .aeruginosa isolates were not resistant to imipenem or aminoglycoside antibiotic ,but highly resistant to the third generation cephalosporins . E .coli strains were not resistant to imipenem or piperacillin‐tazobactam .A .baumannii strains were all multi‐drug resistant . At least 40% of these strains were resistant to imipenem ,aminoglycosides or the fourth generation cephalosporins .All the 4 were gram negative bacilli in Department of Respiratory Medicine ,mainly non‐fermentative bacteria and Enterobacteriaceae , most of which were multi‐drug resistant .MRSA is becoming an important pathogenic bacteria .The prevalence of E .coli is significantly different between the pneumonia associated with cerebral hemorrhage and that associated with cerebral infarction .

Objective To investigate the levels of hypersensitive C-reactive protein(hs-CRP)and serum lipid in patients with acute cerebral infarction.Methods A total of 300 patients with acute cerebral infarction and 300 healthy subjects (normal controls)were included in this study.The levels of hypersensitive C -reactive protein and serum lipid were measured and analyzed via statistics soft.Results The levels of hs-CRP,total cholesterol, triglyceride,low density lipoprotein and high density lipoprotein in the research group were (25.9 ±5.2)mmol/L, (7.5 ±2.3)mmol/L,(2.8 ±0.21)mmol/L,(0.8 ±0.1)mmol/L and (3.3 ±0.2)mmol/L respectively;The levels of hs-CRP,total cholesterol,triglyceride,low density lipoprotein and high density lipoprotein in the control group were (6.3 ±1.2)mmol/L,(4.2 ±0.2)mmol/L,(1.5 ±0.2)mmol/L,(2.2 ±1.5)mmol/L and(2.5 ±0.5)mmol/Lrespectively.There was a significant statistically difference between the two groups(t =5.9,5.1,4.8,4.6 and 4.5,all P <0.05).Multivariate Logistic regression analysis showed that total cholesterol,triglyceride,low density lipoprotein and high density lipoprotein hs-CRP were independent risk factors for the acute cerebral infarction (beta respectively 0.53,0.68,0.69,1.20,0.69;S.E were 0.23,0.16,0.28,0.24,0.25,OR values were 5.6,4.8,6.2,7.8,9.2;95%CI were 0.69 -36.8,0.57 -35.7,0.58 -38.5,0.68 -44.2,0.21 -48.2,all P <0.01).Conclusion The abnormal metabolism of serum lipid and C -reactive protein were independent risk factors in patients with acute cere-bral infarction,which should be positively treated about this.

BACKGROUND:Owing to containing large amounts of growth factors, platelet-rich plasma has been widely used in bone regeneration, wound healing, and so on, while few studies have been reported on cartilage tissue engineering. OBJECTIVE:To explore the effects of platelet-rich plasma on the proliferation and differentiation of chondrocytes, and the feasibility of constructing tissue-engineered cartilage by the combination of chondrocytes and platelet-rich plasma. METHODS:We detected the concentrations of transforming growth factor-β, insulin-like growth factor-1, platelet-derived growth factor and epidermal growth factor BB in the whole blood, platelet-rich plasma, and activated platelet-rich plasma. The rabbit articular chondrocytes were cultured in 10%, 15%, 20%, 30%platelet-rich plasma for 7 days, then the cellproliferation was tested by cellcounting kit-8, and cartilage-related genes (col agen type II, Aggrecan, Sox-9) were determined using QT-PCR. The chondrocytes/platelet-rich plasma composite was implanted subcutaneously into the rabbits, and the samples were harvested after 6 weeks of transplantation for histological examination. RESULTS AND CONCLUSION:The levels of different growth factors in the platelet-rich plasma were higher than those in the whole blood (P<0.05), but lower than those in the activated platelet-rich plasma (P<0.05). Platelet-rich plasma at different concentrations promoted the proliferation of chondrocytes. When the concentration of platelet-rich plasma was no more than 20%, the proliferation of chondrocytes showed a concentration-dependent manner. The 20%platelet-rich plasma showed the best effects to promote the expression of col agen type II (P<0.05), while the 15%platelet-rich plasma could maximize the expression of Sox-9 and Aggrecan (P<0.05). After transplantation of chondrocytes/platelet-rich plasma composite, cartilage-like tissue formed with cartilage lacuna-like structures, and rich extracellular matrix was found, which indicates platelet-rich plasma can be used as an injective scaffold in cartilage tissue engineering.

In order to understand the academic attitude of medical postgraduates,a questionnaire survey was conducted among full-time postgraduate students.The impacts of working experience,gender,marriage status and source on the academic attitude were analyzed,and the improved suggestion and presumption for academic attitude were provided.

Objective To study the chemical constituents from Clerodendrum inerme.Methods The constituents were isolated and repeatedly purified on silica gel column chromatography and their structures were elucidated by spectral analysis.Results Eight compounds: friedelin (Ⅰ), stigmasterol (Ⅱ), betulinic acid (Ⅲ), acacetin (Ⅳ), syringic acid (Ⅴ), p-methoxybenzoic acid (Ⅵ), apigenin (Ⅶ), and daucosterol (Ⅷ) were isolated from the aerial part of C.inerme. Conclusion Compounds Ⅱ, Ⅲ, Ⅴ, Ⅵ, and Ⅷ are isolated from C.inerme for the first time.

Objective:To investigate if injury-related myofibroblast induction could occur in bronchial epithelial cells and the role of TGF-?_1(rhTGF-?_1) in epithelial-myofibroblast transdifferentiation. Methods:Cultured bronchial epithelial cells, 16HBE-14o, were exposed to poly-L-arginine(PLA). Using a immunohistochemical method, ?-smooth muscle actin (?-SMA) expression was detected in those cells after being treated with PLA . Relative activity of chloramphenicol acetyltransferase(CAT) was assessed in 16HBE-14o cells, which were transiently transfected with VSMP8 plasmid containing ?-SMA promoter linked to CAT gene, at different time points after rhTGF-?_1(10 ?g/L) treatment. Results:①?-SMA positive, spindle-shaped cells emerged among the regenerated 16HBE-14o epithelial cells following damage. The number of ?-SMA positive 16HBE-14o cells increased and cell repair was delayed under rhTGF-?_1 stimulation.② CAT activity increased significantly in VSMP8 plasmid transfected 16HBE-14o cells 24 h after TGF-?_1 stimulation. (P

Objective To study the phenotype profiles of bone marrow micrometastatic tumor cells in patients with breast cancer. Methods Immunocytochemical staining was used to study the biologic markers of disseminated tumor cells, and U-PA activity of the plasma in the bone marrow was assayed in breast cancer patients.Results Judged by positive immunocytochemical staining of EMA and CK19, bone marrow micrometastasis was found in 30 out of 72 (41 67%) breast cancer patients. The primary tumor size (? 2=6 417, P=0 040) and p53 protein expression (? 2 =5 930, P=0 025) were significantly correlated with the incidence of micrometastasis. Disseminated tumor cells expressed low protein cyclinD1, p53,Ki-67, EGFR, and high protein p21. Tumor size and axillary lymph node status were found to be significantly correlated with the u-PA activity level. Conclusion Disseminated tumor cells in the bone marrow was in a status of low-cycling and low-proliferation.

OBJECTIVE: To standardize the management on test of hospital preparations to ensure the quality of preparations. METHODS: The Access database and Word application of in Microsoft Office automatic groupware were integrated organically using VBA programming, and the data management system suitable for hospital preparation test was designed. RESULTS: The print files of various primary records and reports related to testing work exhibited beautiful page and uniformed format. Testing data of preparations were included into data base by computer recording, which facilitated data inquiry and data processing related to quality of hospital preparations. CONCLUSION: Due to this system, both the efficiency and quality of hospital preparation test work have been enhanced, which contributes to institutionalization and standardization of hospital preparation work.