1.Etiology and treatment strategy of refractory periapical periodontitis.
Jun-qi LING ; Xi WEI ; Hong-yan LIU
Chinese Journal of Stomatology 2010;45(1):52-57
Actinomyces
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isolation & purification
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Actinomycosis
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microbiology
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Apicoectomy
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methods
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Candida albicans
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isolation & purification
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Candidiasis
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microbiology
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Enterococcus faecalis
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isolation & purification
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Foreign Bodies
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complications
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Gram-Positive Bacterial Infections
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microbiology
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Humans
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Microsurgery
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methods
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Periapical Periodontitis
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etiology
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microbiology
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surgery
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therapy
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Radicular Cyst
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complications
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Root Canal Filling Materials
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therapeutic use
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Root Canal Therapy
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methods
2.Progress in study of spectrum-effect relationship of traditional Chinese medicine and discussions.
Ling-jun ZENG ; Bing LIN ; Hong-tao SONG
China Journal of Chinese Materia Medica 2015;40(8):1425-1432
Spectrum-effect relationship of traditional Chinese medicine is a scientific method based on fingerprint of traditional Chinese medicine, which studied the correlations between fingerprint and activity. The method revealed the activity related peaks and clarified the active components. It provided directions and thoughts for the clarification of pharmacodynamic material basis and establishment of evaluation method to reflect the inherent quality of traditional Chinese medicine. In this text we would make a systematic review about the progress in the study of spectrum-effect relationship of traditional Chinese medicine after summarized the latest years of investigations from researchers at home and abroad, including the establishment of fingerprint, efficacy evaluation, and data processing. The key problems in each part were clarified and corresponding discussions were made, providing thoughts and advices for the following study of spectrum-effect relationship of traditional Chinese medicine. At last we made a expecting on the development trend of spectrum-effect relationship of traditional Chinese medicine.
Animals
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Drugs, Chinese Herbal
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chemistry
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pharmacology
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Humans
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Medicine, Chinese Traditional
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Plants, Medicinal
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chemistry
3.Effect of acetamide on cardiac troponin I of rats with tetramine poisoning.
Yu-Jun MENG ; Jian-Ling SU ; Hong-Shun ZHANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2008;26(11):668-669
Acetamides
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pharmacology
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Animals
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Bridged-Ring Compounds
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poisoning
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Disease Models, Animal
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Female
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Male
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Rats
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Rats, Sprague-Dawley
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Troponin I
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blood
4.Protective effect of transection of cervical sympathetic trunk on fetal brain in rats with pregnancy-induced hypertension
Li-Hong ZHANG ; Ling MA ; Jian-Jun CUI ;
Chinese Journal of Anesthesiology 1994;0(01):-
Objective To investigate the protective effect of transection of cervical sympathetic trunk (TCST)on the fetal brain in rats with pregnancy-induced hypertension(PIH).Methods Thirty-two pregnant Wistar rats were randomly divided into 4 groups(n=8 each):A control group;B PIH group;C TCST+PIH group and D sham operation+PIH group,PIH was produced by L-NAME 12.5 mg?100g~(-1) given subcutaneously from 14~(th)-20~(th) day of gestation.In group A normal saline was given instead of L-NAME.In group C TCST was performed on the 14~(th) day of gestation and L-NAME was injected asin group B.In group D the cervical sympathetic trunk was only exposed but not cut.Caesarean section was performed and fetus was taken on the 21~(st) day of gestation.The ultrastructure of fetal brain was examined.The content of ATP,ADP and AMP in the fetal brain and the Na~+-K~+-ATPase and Ca~(2+)-ATPase activity in neuronal plasmalemma were determined.Results The ultrastructure of the fetal brain was almost normal in group A and C,but was seriously damaged in group B and D.The ATP and adenylic acid content in the brain tissue and the Na~+-K~+-ATPase and Ca~(2+)-ATPase activity in neuronal plasmalemma were significantly lower in group B and D than in group A and C.Conclusion TCST has protective effect on the fetal brain in rats with PIH by improving energy supply and enhancing pump function of neuron.
5.Relationship between Fatty Liver and Blood Lipids in Children with Simple Obesity
shao-ling, WANG ; yong-hong, CHEN ; jun-bao, DU
Journal of Applied Clinical Pediatrics 2004;0(07):-
Objective To study the relationship between fatty liver and blood lipids in children with simple obesity and the precaution of this disease.Methods Forty-eight cases from 2 to 16 years old with simple obesity were divided into 2 groups.The first group included 19 cases with fatty liver(38.78%);the second group included 29 cases without fatty liver(61.22%). Blood lipids in 2 groups were analyzed with biochemical method.Blood total cholesterol(TC),triglyeride(TG),high density lipoprotein(HDL) and low density lipoprotein(LDL) were detected and the liver was examined with ultrasound.Results Blood TC,TG,HDL and LDL in cases with fatty liver were higher than those wit-hout fatty liver.Blood TG had the closest relation with fatty liver(P_a
6.Effect of speech therapy on mental health of patients with aphasia
Xu HE ; Jun HONG ; Jian-hua LIN ; Ling QIU
Chinese Journal of Rehabilitation Theory and Practice 2002;8(11):694-695
ObjectiveTo investigate the effect of speech therapy on mental health of patients with aphasia. MethodsCRRCAE and SCL-90 were used to assess the speech and mental state in 28 aphasia patients. All aphasia patients were treated by using speech therapy and the changes before and after treatment were compared. ResultsThe scores in listening comprehension, repetition, naming, reading,reading comprehension, writing and counting were distinctly higher at post-treatment than that at pre-treatment. All SCL-90 factor's levels at post-treatment were distinctly reduced than that at preat post-treatment(P<0.01).Conclusions Speech therapy can improve the language function,as well as mitigate the mental symptoms.
7.Briefly on clinical medication features of professor LIU Bi-chen
Rong-Qian XU ; Tao-Yu SUN ; Hong-Ling WANG ; Hong ZHANG ; Jun-Hong WANG ;
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(10):-
Professor LIU Bi-chen has 60 years' experience in the traditional Chinese medicine.He possesses the unique theories,rich clinical experience and excellent clinical effect.He has made contributions to the Pediatrics of traditional Chinese medicine.The article tries to summarize the essence of LIU Bi-chen's clinical experience,as to show the regulation and characteristics and give introduction to the reader.it is mainly consists of the thought of cure from the lung、shaoyang theory、medicinal formular、applied of arctium fruit.etc.
8.Cell proteins that potentially interact with HBV polymerase were identified by co-immunoprecipitation-based LC-MS/MS identification and IPA.
Meng-ting LING ; Jun-yuan GONG ; Jun-wu LI ; Tian-hong ZHOU
Chinese Journal of Virology 2014;30(6):636-644
Hepatitis B virus (HBV) is a major cause of chronic liver disease, and frequently results in hepatitis, cirrhosis, and ultimately hepatocellular carcinoma. HBV polymerase (Pol) is an essential viral protein that is important for HBV replication and might be involved in the development of hepatocellular carcinoma. Protein-protein interactions appears to be crucial for its role. The aim of this study was to screen and identify the proteins that interact with Pol using a co-immunoprecipitation-based LC-MS/MS identification technique. The HBV Pol gene was amplified by polymerase chain reaction (PCR) and cloned into pCDNA3.1(+). The recombinant plasmid pCDNA3. 1(+)-Pol-flag was transfected into HeLa cells. Liquid chromatography and tandem mass spectrometry (LC-MS/MS) identified 45 proteins that co-immunoprecipitated with flag-tagged HBV Pol. Eleven of these have previously been reported as proteins that interact with HBV Pol. A proof-of-concept-based Ingenuity Pathway Analysis (IPA, www.ingenuity.com) was used to characterize the functions and pathways of these 45 identified proteins and HBV Pol. Among these proteins, four proteins may play a role in three major molecular cellular networks, and are therefore worthy of further investigation.
Cell Line, Tumor
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Chromatography, Liquid
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methods
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Gene Products, pol
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chemistry
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genetics
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metabolism
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Hepatitis B
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genetics
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metabolism
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virology
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Hepatitis B virus
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chemistry
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enzymology
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genetics
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Humans
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Immunoprecipitation
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methods
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Protein Interaction Maps
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Software
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Tandem Mass Spectrometry
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methods
9.FAK gene silencing induces apoptosis of leukemic cells in vitro
Lv-Hong XU ; Jian-Pei FANG ; Yi LE ; Wen-Jun WENG ; Dong-Ling HONG ;
Chinese Journal of Pathophysiology 2000;0(07):-
AIM:Targeting of focal adhesion kinase (FAK) gene,we aim to construct FAK shRNA lentiviral vector and to identify its function on the growth of leukemic cells.METHODS:FAK shRNA was chemically synthesized,and inserted into a GFP-lentiviral plasmid through molecular biology methods.After packaged and concentrated,the lentiviral-FAK-shRNA-vector was transduced into a human leukemic cell line.FAK gene expression was detected by reverse transcriptional PCR and Western blotting.Cell apoptosis was measured by Annexin V labeling.RESULTS:The results showed that FAK shRNA was successfully inserted into the lentival vector,and the infection efficiency varied from 10% to 25%.Compared to the control vector (lentival vector without FAK shRNA),FAK shRNA inhibited the expression of FAK mRNA and protein by 40% and 70%,respectively.Moreover,the results of apoptosis experiment showed that the percentages of Annexin V+ cells in control vector group and FAK shRNA group were (4.19 ? 0.36) % and (8.48 ? 0.58) % respectively,the difference was statistically significant (P
10.Effect and safety of L-carnitine in the treatment of idiopathic oligoasthenozoospermia: a systemic review.
Xue-jun SHANG ; Ling-ling WANG ; Dun-sheng MO ; Hong-cai CAI ; Da-dong ZHENG ; Yuan-zhong ZHOU
National Journal of Andrology 2015;21(1):65-73
OBJECTIVETo evaluate the effect and safety of L-carnitine in the treatment of idiopathic oligoasthenozoospermia based on current clinical evidence.
METHODSWe searched the Cochrane Library, PubMed, MEDLINE, EMBASE, CNKI, VIP, CBM and Wanfang Database from the establishment to April 2014 for the published literature on the treatment of idiopathic oligoasthenozoospermia with L-carnitine. We conducted literature screening, data extraction, and assessment of the methodological quality of the included trials according to the inclusion and exclusion criteria, followed by statistical analysis with the RevMan 5. 2 software.
RESULTSSeven randomized controlled trials involving 751 patients with idiopathic oligoasthenozoospermia met the inclusion criteria, and 678 of them were included in the meta-analysis. L-carnitine treatment achieved a significantly increased rate of spontaneous pregnancy as compared with the control group (RR = 3.2, 95% CI 1.74 to 5.87, P = 0.0002). After 12-16 and 24-26 weeks of medication, total sperm motility (WMD = 5.21, 95% CI 2.78 to 7.64, P < 0.0001 and WMD = 9.29, 95% CI 1.28 to 17.29, P = 0.02) and the percentage of progressively motile sperm (WMD = 12.44, 95% CI 4.58 to 20.31, P = 0.002 and WMD = 9.76, 95% CI 3.56 to 15.97, P = 0.002) were remarkably higher than those in the control group, but no statistically significant differences were observed in sperm concentration between the two groups (WMD = 4.91, 95% CI -2.63 to 12.45, P = 0.2 and WMD = 0.93, 95% CI -3.48 to 5.34, P = 0.68). After 12-16 weeks of treatment, the percentage of morphologically abnormal sperm was markedly decreased in the L-carnitine group as compared with the control (WMD = -2.48, 95% CI -4.35 to -0.61, P = 0.009), but showed no significant difference from the latter group after 24-26 weeks (WMD = -4.38, 95% CI -9.66 to 0.89, P = 0.1). No statistically significant difference was found in the semen volume between the two groups after 12-16 or 24-26 weeks of medication (WMD = -0.13, 95% CI -0.43 to 0.18, P = 0.42 and WMD = 0.28, 95% CI -0.02 to 0.58, P = 0.07). No serious L-carnitine-related adverse events were reported in 4 of the randomniized controlled trials.
CONCLUSIONThe current evidence indicates that L-carnitine can improve spontaneous pregnancy and semen parameters in the treatment of idiopathic oligoasthenozoospermia, with no serious adverse reactions.
Asthenozoospermia ; drug therapy ; Carnitine ; adverse effects ; pharmacology ; Female ; Humans ; Male ; Pregnancy ; Pregnancy Rate ; Randomized Controlled Trials as Topic ; Semen Analysis ; Sperm Count ; Sperm Motility