Objective To explore the expression and clinical significance of matrix metalloproteinase-9 (MMP-9) in gastric cancer tissues.Methods The expressive level of MMP-9 in normal gastric mucosa tissues,para-carcinoma tissues,and cancer tissues were determined with histochemistry streptavidin-perosidase (SP) methods.The relationship of MMP-9 and gastric cancer tissue were analyzed.Results The positive expression rate of MMP-9 in the gastric cancer tissues was significantly higher than that in normal gastric mucosa tissues and para-carcinoma tissues (x2 =86.66,P ＜0.01).The positive expression rate of MMP-9 in gastric cancer tissues was related to differentiated degree of cancer tissues,tumor node metastasis (TNM) stage,infiltration degree,and lymphatic metastasis (x2 =7.76,P ＜ 0.05; x2 =12.94,54.37,11.16,P ＜ 0.01).Conclusions MMP-9 probably participates in the occurrence and development of a gastric cancer,and is related to its invasion and metastasis.

Platelet-derived growth factor(PDGF), as an important cytokine, can induce mesangial cell proliferation and accumulate extracellular matrix. The proliferation of the mesangial cells correlate with the cell cycle regulation. The different isoform of PDGF works on mesangial cells to produce different effects. This review summarizes the relevant mechanism about PDGF inducing proliferation of mesangial cells.

Objective:Observe the curative effects of transcutaneous electrical stimulation (TES) combined with medicine when it is used to treat the multi-infarct dementia (MID). Method: 54 dementias are randomly divided into two groups: The group TES and the matched group, with each 27 patients, in which, the group TES adopts TES with medicine and the matched group is only supplied to medicine. Score the MMSE, MBI and HDS before and after treatment. Judge the curative effects according to the scores of MBI. Results: In comparison, the MMSE, MBI and HDS are greatly different, the curative efficiency of the group tES is higher than the one of the matched group and the difference is significance in statistic(P

Objective:To develop a novel amperometic immunosensor for the determination of Urinary Trypsinogen-2(TSG-Ⅱ).Methods:the immunosensor was developed by modifying Nafion film on the glassy carbon electrode(GCE),then thionine and colloidal gold were immobilized by static electricity and covalent bonding self-assembly.And then the antibody of Urinary TSG-Ⅱwas absorbed on the surface of nano-gold monolayer.Finally,Horseradish peroxidase(HRP) blocked the non-specific adsorption site on the electrode.Result:Experiment results showed that the good linear relations between peak current and concentration of TSG-Ⅱ were established in the range of 6.0-100?g/L.The detection limit was 3.5?g/L.Conclusion:The amperometie immunoassay biosensor has high sensitivity and good specificity and it has laid the foundation to establish a new method for detection of TSG-Ⅱ.

3.5cm in diameter were given multiple overlapping ablations that encompassed the entire les- ions.The operation was performed under local anesthesia in association with systemic analgesia. Results:The procedure was successfully accomplished in all the 16 patients. Adverse reactions happened at 2 ～5 postoperative days,including local pain and discomfort,pyrexia,and elevations of ALT levels. No bile leakage or hemorrhage occured.Follow-up observations in 16 patients for 1～3 years (mean,1 years and 8 months)showed a complete response (CR)in 15 patients and a partial response (PR)in l patient by one mission of RFA,the effective rate (CR+PR)being 100%.The hepatic hemangiomas decreased by a mean of 62.0%(range,50.1%～84.7%)in diameter. Conclusion:RFA is a safe and effective therapy for liver hemangiomas in properly selected patients by experienced hands.

Objective:To study three-dimensional hepatic microcirculation in normal rabbits.Methods:The corrosion casts was produced using methyl methacrylate and the HA,PV and BD were observed by scanning electron microscopy(SEM).Results:The diameter range of hepatic sinus was about 9.5～10.9?m,the ratio of HA/PV,HA/BD and PV/BD were about 0.28?0.03,0.26?0.06 and 0.89?0.12.The ratio of hepatic sinus and cells was about 31.87%.There was no significant rheological respectively between entering and outflowing liver blood.Conclusion:It is an effective method to study the hepatic microcirculation by using both methylmethacrylate and scanning electron microscopy.The results can serve as contrast standard for hepatic pathological research.

Objective To study the effects of protein kinase C (PKC) inhibitor staurosporine (STS) on the proliferation and apoptosis of human cholangiocarcinoma QBC-939 cells and to explore its possible mechanism. Methods CCK-8 was used to detect the effects of PKC inhibitor STS on the proliferation of human cholangiocarcinoma QBC-939 cells. The effects of STS on the ultrastructural characteristics of QBC-939 cells were observed by routine transmission electron microscopy (TEM). The apoptosis rate and the cell cycle distribution of QBC-939 cells were detected by flow cytometry. The expression of cyclin B1,Cdk1 and p-Cdk1 in QBC-939 cells was detected by Western blotting. Results STS could significantly inhibit the proliferation of QBC-939 cells in a dose-dependent manner (P <0. 05) and the half inhibitory concentration ( IC50 ) of QBC-939 cells at 24th and 48th h was 334 nmol·L-1 and 118 nmol · L-1 , respectively. TEM observed that STS could induce typical apoptotic bodies and super-microstructural changes of QBC-939 cells. By Annexin V-FITC/ PI double labeling flow cytometry,we found that the apoptotic rate of QBC-939 cells after treatment with STS for 0,12,24 and 48 h was (10. 16±4. 52)% ,(22. 35±2. 19)% ,(34. 27±2. 30)% and (59. 70±5. 97)% ,respectively. By flow cytometry,compared with the control group,STS could significantly increase apoptosis rate of QBC-939 cells,decrease the percentage of cells in G0 / G1 phase and increase the percentage of cells in G2 / M phase (P<0. 05). Western blotting proved that the expression levels of cyclin B1 and Cdk1 proteins in the STS-treated QBC-939 cells were significantly decreased (P<0. 05),while the expression level of p-Cdk1 protein in the STS-treated QBC-939 cells was significantly increased ( P < 0. 05 ). Conclusion STS can significantly inhibit cell proliferation and induce apoptosis of human cholangiocarcinoma QBC-939 cells and the mechanism may be related to cell cycle arrest at G2 / M phase.

Objective To investigate the influence of maternal and child care bedside on early development of newborns and maternal depression and anxiety,lactation.Methods From January 2013 to June 2015,120 cases of healthy full -term newborns and maternal admitted in our hospital were randomly divided into control group and observation group,60 cases in each group.The two groups of newborns and mothers were given routine care,including care and neonatal umbilical buttocks care,maternity care,including genital or abdominal incision dressing,newborns were routinely vaccinated.In the control group,the newborns were taken to a public bath chamber concentrated bath. The observation group was implemented maternal and child care bedside manner Based on this,at the bedside of the newborn bathing and massage,swimming daily with newborns.Anxiety score (SAS)and depression scale score (SDS)for depression and anxiety were evaluated before and after the two groups of mothers nursing,and maternal milk yield variation were observed at different time,measured neonatal neurological assessment score (NBNA)and height,body mass index,head circumference growth.Results After treatment,the SAS and SDS scores of mothers of the two groups were decreased compared with before treatment,which in the observation group decreased more significantly, the differences were statistically significant (t =3.46,4.21,all P <0.05).After care,the maternal milk productions of the two groups started to grow,which of the observation group were always increased better than the control group, the differences were statistically significant (χ2 =9.34,4.53,6.79,all P <0.05).The newborn NBNA scores,head circumference,height,improvement in the quality index of growth in the observer group were better than the control group,the differences were statistically significant (t =1.98,1.65,2.73,3.63,all P <0.05).Conclusion Maternal and child bedside care can significantly improve maternal depression and anxiety scores,increase maternal lactation, and has a certain role in promoting early neonatal development,it is worth clinical application.

Students will be familiar with the major contents of clinical laboratory management,such as clinical laboratory quality management,laboratory creature safety containment,clinical laboratory accredit action through the course of clinical laboratory management for laboratory medicine students.Meanwhile,by continuously perfecting the teaching contents and methods,students' satisfaction with this course has been improved,and they pay more attention and give higher appraisement to this course.

Training of doctor receiving in-service training is the important part of clinical education in teaching hospital. It will improve the clinical and scientific research ability of the doctor receiving in-service training and will train high-quality medical persons for primary hospital by training before going to post,clinical teaching,surgical operation teaching,standardized special lecture,case report and various kinds of science activities.