Objective To investigate the effect of Shuxuetong injection on anticoagulation of nephrotic syndrome.Methods 42 patients were randomly divided into 2 groups:Shuxuetong group and control group.The Shuxuetong group were injected Shuxuetong injection while the control group were injected low molecular dextran and Danshen injection.Hemorheology and clotting index were detected before and after treatment.Results Hemorheology and clotting index of the two groups were improved.Hemorheology and clotting index of the Shuxuetong group were improved better than that of the control group.Conclusions The results suggested that the Shuxuetong injection was a safe and effective anticoagulation drug for the treatment of nephrotic syndrome.

Adult ; Anemia, Aplastic ; chemically induced ; immunology ; therapy ; Benzene ; poisoning ; Cyclosporine ; therapeutic use ; Female ; Humans ; Immunity, Cellular ; Immunosuppressive Agents ; therapeutic use ; Male ; Middle Aged

Objective To investigate the diagnosis value of laparosco-pie examination in ascites.Methods The data of twenty-three cases with ascites of unknown origin undergoing laparescopie examination and pathologic examination were retrospectively analyzed from May 2004 to August 2008.Results Twenty-two eases were definitely diagnosed by laparoscopie exploration,the positive rate was 95.6%.One ease re-mained unknown.Among them,18 eases were tuberculous peritonitis; 3 eases were metastatic adenocarcino-ma; one ease was peritoneal mesothelioma.No obvious postoperative complications occurred.Conclusion Laparoscopie examination is a safe method with hige definite diagnostic rate,which has important values in diagnosis of ascites of unknown origin.

Objective: To study the significance of intracellular free calcium levels ([Ca 2+]i) and protein kinase C(PKC) levels in the process of human salivary gland mucoepidermoid carcinoma MEC-1 cell apoptosis induced by adriamycin. Methods: MEC-1 cells were treated with adriamycin at 10 ?mol/L for 30 s～24 h.Apoptosis of the cells was investigated by light and electron microscopy, agarose gel electrophoresis and flow cytometry. [Ca 2+]i was determined by flow cytomerty, PKC by Bardford method. Results: The results showed that MEC-1 cells presented classic morphologic features of apoptosis. [Ca 2+]i in the treated cells was increased from (36.63?0.61) nmol/L to (84.00?0.45) nmol/L after 30 s～24 h treatment,while that in the control cells was 17.43?0.47 (P

Objective To investigate the transactivating effect of hepatitis C virus(HCV)core protein on inducible nitric oxide synthase(iNOS)gene promoter and the molecular biological mecha- nisms of HCV pathogenesis.Methods Polymerase chain reaction(PCR)technique was employed to amplify the sequence of iNOS promoter by using HepG2 genomic DNA as template,and the product was cloned into pGEM-T vector.The iNOSp gene was cut from T-iNOSp by KpnⅠand XhoⅠ,and then was cloned into pCAT3-Basic,the constructed vector was named as pCAT3-iNOSp,pCAT3-iN- OSp was transfected into the LO_2 cell line.LO_2 cell was also cotransfected with pcDNA3.1(-)-core and pCAT3-iNOSp by FuGENE 6 transfection reagents.The LO_2 cells transfected with pCAT3-Basic was used as negative control.The activity of CAT in LO_2 cells was detected by an ELISA kit after 48 hours,which reflected the transactivating function of HCV core protein to iNOS gene promoter.Re- sults The expressive vector pcDNA3.1(-)-core and report vector pCAT3-iNOSp had been construc- ted and confirmed by restriction enzyme digestion and sequencing.The expression of CAT in LO_2 cells transfected with pCAT3-iNOSp and peDNA3,1(-)-core was 11 times as higher as that of pCAT3-bas- ic,and 6 times as higher as that of pCAT3-iNOSp.Conclusion It is suggested that HCV core protein can transactivate iNOS gene promoter.

Objective To study the feasibility of laparoscopic cholecystectomy accompanied by simultaneous umbilical hernia repair.Methods Twenty-four adult patients received laparoscopic cholecystectomy accompanied by simultaneous umbilical hernia repair in our hospital from November 2003 to April 2008.An incision was made in navel defect and entered the abdominal cavity under a direct vision.LC was performed by 3-trocar technique.Umbilical hernia was repaired by Mayo' style.Results The operation was successfully completed in all the cases.The operation time ranged from 35 to 65 minutes (mean,48 minutes),the blood loss was 5-30 ml(mean,16 ml),and hospital stay after the operation was 3 -4 days.No patient dereloped wound infection or skin necrosis.No recurreme was found during a 1-to 54-month follow-up(mean,28.6 months).Conclusions Laparoscopie cholecystectomy accompanied by simultaneous umbilical hernia repair is a safe and effective procedure.Mayo repair is simple and effective for umbilical hernia with less fascial defects.

In order to make a rational and effective cure measure for lung cancer,people need to establish suitable animal model.Spontaneous or induced model needs long time to form tumor and the rate is not steady.Xenograft mouse model is widely used in laboratories because of its steady tumor formation rate and less time in oncogenesis.Engineered mouse model which is based on genetic technology not only helps us to understand pathological process of lung cancer,but also can provide an ideal preclinical model of targeted therapy experiment,and it will be the important development direction of lung cancer animal model.

Objective To explore the value of characteristic manifestation of diffusion-weighted images at 3.0T MR system in cer-ebral disease.Methods 120 patients with cerebral disease diagnosed by MR diffusion-weighted images (fat suppression effect,T2 blackout effect,T2 shine-through effect,T2 washout effect)underwent routine MR scan,including echo planar imaging-diffusion weighted imaging (EPI-DWI)and apparent diffusion coefficient (ADC)map,whose characteristic manifestations of DWI were retro-spectively analyzed.Results 1 5 cases of lipoma and 8 cases without fat lesions were diagnosed with fat suppression effect.13 cases of acute cerebral hematoma,1 1 cases of hemorrhagic cerebral infarction and 1 6 cases of micro bleeding of missed diagnosis or small cavernous hemangioma were diagnosed with T2 blackout effect.1 5 cases of epidermoid cyst,5 cases of choroid plexus cyst were di-agnosed with T2 shine-through effect.1 5 cases of posterior reversible encephalopathy syndrome and 22 cases of brain tumor were di-agnosed with T2 washout effect.Conclusion Comprehensive use of the characteristic manifestation of EPI-DWI and ADC map can help the diagnosis of cerebral disease.

Objective To investigate the feasibility and safety of hysteroscopic resection of large submucous hysteromyomas. Methods A retrospective study was carried out on 116 patients receiving hysteroscopic resection of submucous hysteromyoma. According to the maximal diameter of the resected myomas, the patients were divided into two groups: the control group,

We have reported previously a peculiar type of neurons in the submucous nerve plexus in the intestine of guinea pigs, and named them tentatively the ′paralymphatic neurons′. In order to further study this type of neurons, the immunohistochemical observation for neuron specific enolase (NSE) reactivity of the enteric nerve plexus in guinea pigs was carried out in this investigation. We found that all the neurons in the enteric nerve plexus showed NSE positive reaction. The paralymphatic neurons located closely against to lymphatic vessels in submucous nerve plexus in small intestine and colon also exhibited same NSE positive reaction as other neurons. All the smooth muscle and connective tissue cells were NSE negative. Most of the paralymphatic neurons exhibited typical morphological features of neurons. Although some cells did not show obvious neuronal characters, but they all presented the same positive reactivity. Hence, this study provide another reliable evidence to confirm the exact neuronal nature of the paralymphatic neurons.