1.Regulation of syringin, chlorogenic acid and 1,5-dicaffeoylquinic acid biosynthesis in cell suspension cultures of Saussurea involucrata.
Ri-Dao CHEN ; Xiao LIU ; Jian-Hua ZOU ; Lin YANG ; Jun-Gui DAI
China Journal of Chinese Materia Medica 2014;39(12):2275-2280
Syringin, chlorogenic acid and 1,5-dicaffeoylquinic acid are three main bioactive ingredients in herbs of Saussurea involucrata with various pharmacological properties, while their contents are very low. In this study, the biosynthesis of syringin, chlorogenic acid and 1,5-dicaffeoylquinic acid in the cell suspension cultures of S. involucrata were regulated by feeding carbon sources and precursors, which resulted in a great increase of the contents and yields of the above three bioactive ingredients. After 16 days of fermentation, the yields of syringin, chlorogenic acid and 1,5-dicaffeoylquinic acid reached 339.0, 225.3, 512.7 mg x L(-1), respectively. Meanwhile, their contents increased up to 67.9, 1.9, 10.6 times of wild medicinal material, respectively. The results provided a solid basis for further studies on application of cell suspension cultures of S. involucrata for large-scale production of bioactive compounds syringin, chlorogenic acid and 1,5-dicaffeoylquinic acid.
Cell Culture Techniques
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Cells, Cultured
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Chlorogenic Acid
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analysis
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metabolism
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Cinnamates
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analysis
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metabolism
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Glucosides
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analysis
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biosynthesis
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Phenylpropionates
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analysis
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Saussurea
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chemistry
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growth & development
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metabolism
2.Removing esophageal and laryngeal foreign bodies with rigid video endoscopy:a retrospective review of 177 cases
Shidong CHU ; Jun CI ; Ying FANG ; Hua GUAN ; Changyang WANG ; Jianjun ZOU
China Journal of Endoscopy 2017;23(1):100-102
Objective Laryngeal and upper esophageal foreign bodies are common diseases, we explored a new and simple method to remove these foreign bodies. Methods The clinical data of 177 patients with laryngeal and upper esophageal foreign bodies from June 2008 to January 2016 were analyzed retrospectively. We used two methods to treat these foreign bodies:130 foreign bodies were directly removed under video endoscopy;47 foreign bodies were removed with suspension laryngoscopy, and video endoscopy was used meanwhile. Result 177 laryngeal and upper esophageal foreign bodies were removed well, without severe complications like esophageal perforation or phyryngeal fistula. Conclusion Removing esophageal and laryngeal foreign bodies with video endoscopy is simple, safe and effective.
3.3D finite element model of human knee injuries in the traffic accident.
Wei-Hua HUANG ; Ping HUANG ; Zheng-Dong LI ; Dong-Hua ZOU ; Yu SHAO ; Hui-Jun WANG ; Yi-Jiu CHEN
Journal of Forensic Medicine 2014;30(1):1-12
OBJECTIVE:
To explore the injury mechanism of the human knee in a traffic accident by establishing a 3D finite element (FE) model.
METHODS:
The FE model, composed of femur, tibia, fibula, patella, meniscus, knee ligaments and surrounding soft tissues, was reconstructed by CT scanning data from a male volunteer. Validation was performed by the lateral impact simulation, and the stress and strain results were obtained to be compared with those previously reported for injury prediction.
RESULTS:
The results derived from the FE model were found to be similar with those previously reported, most of the ligaments and meniscus wounded at 40 m/s collision, which was readily observed.
CONCLUSION
The simulation results generated by FE model can be effectively used for the injury mechanism analysis of initial contact.
Accidents, Traffic
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Biomechanical Phenomena
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Femur
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Finite Element Analysis
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Humans
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Knee Injuries/etiology*
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Knee Joint
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Male
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Models, Theoretical
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Tibia
4.Meroterpenoids and isoberkedienolactone from endophytic fungus Penicillium sp. associated with Dysosma versipellis.
Jun-Wei LI ; Rui-Gang DUAN ; Jian-Hua ZOU ; Ri-Dao CHEN ; Xiao-Guang CHEN ; Jun-Gui DAI
Acta Pharmaceutica Sinica 2014;49(6):913-920
Seven meroterpenoids and five small-molecular precursors were isolated from Penicillium sp., an endophytic fungus from Dysosma versipellis. The structures of new compounds, 11beta-acetoxyisoaustinone (1) and isoberkedienolactone (2) were elucidated based on analysis of the spectral data, and the absolute configuration of 2 was established by TDDFT ECD calculation with satisfactory match to its experimental ECD data. Meroterpenoids originated tetraketide and pentaketide precursors, resepectively, were found to be simultaneously produced in specific fungus of Penicillium species. These compounds showed weak cytotoxicity in vitro against HCT-116, HepG2, BGC-823, NCI-H1650, and A2780 cell lines with IC 50 > 10 micromol x L(-1).
Berberidaceae
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microbiology
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Cell Line
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Cell Line, Tumor
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Humans
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Lactones
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isolation & purification
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pharmacology
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Monoterpenes
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isolation & purification
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pharmacology
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Penicillium
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chemistry
7.Isolation and analysis of syringin in cell suspension cultures of Saussurea involucrata.
Ri-dao CHEN ; Jian-hua ZOU ; Jing-ming JIA ; Jun-gui DAI
Acta Pharmaceutica Sinica 2009;44(4):436-439
Syringin is one of the main bioactive ingredients in Saussurea involucrata. In this study, various chromatographic techniques were employed to isolate and purify syringin in the polar extraction of cell suspension cultures of S. involucrata. The structure of syringin was characterized by the analysis of spectroscopic data. A quantitative analytical method for the content of syringin in cultures of S. involucrata was established with RP-HPLC. The method is convenient, accurate and reliable. All this results provided a basis for further studies on application of cell suspension cultures of S. involucrata for large-scale production of bioactive compound syringin.
Cell Culture Techniques
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Chromatography, High Pressure Liquid
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methods
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Glucosides
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analysis
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isolation & purification
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Phenylpropionates
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analysis
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isolation & purification
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Plants, Medicinal
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chemistry
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cytology
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Reproducibility of Results
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Saussurea
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chemistry
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cytology
8.Compensatory function of bradykinin B1 receptor in the inhibitory effect of captopril on cardiomyocyte hypertrophy and cardiac fibroblast proliferation in neonatal rats.
Jun ZOU ; Jiang-hua REN ; Dan FENG ; Hong WANG ; Jiang XU
Chinese Medical Journal 2008;121(13):1220-1225
BACKGROUNDBradykinin (BK) acts mainly on two receptor subtypes: B(1) and B(2), and activation of B(2) receptor mediates the most well-known cardioprotective effects of angiotensin converting enzyme inhibitors (ACEi), however, the role that B(1) receptor plays in ACEi has not been fully defined. We examined the role of B(1) receptor in the inhibitory effect of ACE inhibitor captopril on rat cardiomyocyte hypertrophy and cardiac fibroblast proliferation induced by angiotensin II (Ang II) and explored its possible mechanism.
METHODSNeonatal cardiomyocytes and cardiac fibroblasts (CFs) were randomly treated with Ang II, captopril, B(2) receptor antagonist (HOE-140) and B(1) receptor antagonist (des-Arg(10), Leu(9)-kallidin) alone or in combination. Flow cytometry was used to evaluate cell cycle, size and protein content. Nitric oxide (NO) and intracellular cyclic guanosine monophosphate (cGMP) level were measured by colorimetry and radioimmunoassay.
RESULTSAfter the CFs and cardiomyocytes were incubated with 0.1 micromol/L Ang II for 48 hours, the percentage of CFs in the S stage, cardiomyocytes size and protein content significantly increased (both P < 0.01 vs control), and these increases were inhibited by 10 micromol/L captopril. However, NO and cGMP levels were significantly higher than that with Ang II alone (both P < 0.01). 1 micromol/L HOE-140 or 0.1 micromol/L des-Arg(10), Leu(9)-kallidin attenuated the effects of captopril, which was blunted further by blockade of both B(1) and B(2) receptors.
CONCLUSIONSActing via B(2) receptor, BK contributes to the antihypertrophic and antiproliferative effects of captopril on cardiomyocytes and CFs. In the absence of B(2) receptor, B(1) receptor may act a compensatory mechanism for the B(2) receptor and contribute to the inhibition of cardiomyocyte hypertrophy and CFs proliferation by captopril. NO and cGMP play an important role in the effect of B(1) receptor.
Angiotensin-Converting Enzyme Inhibitors ; pharmacology ; Animals ; Animals, Newborn ; Captopril ; pharmacology ; Cardiomegaly ; prevention & control ; Cell Proliferation ; drug effects ; Cell Size ; drug effects ; Cyclic GMP ; analysis ; DNA ; biosynthesis ; Fibroblasts ; drug effects ; physiology ; Myocytes, Cardiac ; drug effects ; pathology ; Nitric Oxide ; analysis ; Rats ; Rats, Sprague-Dawley ; Receptor, Bradykinin B1 ; physiology
9.The effect of four kinds of traditional Chinese herbal compound on the experimental acute stress behaviors and the hypothalamus-pituitary-adrenal gland axis.
Yao TONG ; Jun ZOU ; Li-qiang NI ; Shang-hua LIANG ; Bi-yin CAO ; Hui-juan CHEN
China Journal of Chinese Materia Medica 2005;30(23):1863-1866
OBJECTIVETo study the effects of four kinds of traditional Chinese herbal compound, Sini powder, Banxiahoupu soup, Ganmaidazao soup and Guizhigancaolonggumuli soup, on the experimental acute stress behaviors and the hypothalamus-pituitary-adrenal gland axis.
METHODRats were divide into 7 groups: normal group, model 1 and 2 groups, Sini powder group, Banxiahoupu soup group, Ganmaidazao soup group, and Guizhigancaolonggumuli soup group. The behaviors of the rats and their CRH of the hypothalamus, ACTH of plasma and CORT of serum (by the means of radio-immunity) were detected.
RESULTSini powder could reduce the crossing times of open-field test( P < 0.01) and the contents of CRH and CORT (P < 0.01, P < 0.05); Banxiahoupu soup could extent the still time of tail-hanging test ( P < 0.05) and the content of CRH (P < 0.05); Ganmaidazao soup could prolong the still time (P < 0.05) and reduce the struggle times (P < 0.01) of tail-hanging test and reduce the contents of CRH, ACTH and CORT (P < 0.01, P < 0.05); Guizhigancaolonggumuli soup could reduce the crossing times of open-field test (P < 0.01) , extent the still time (P < 0.05) and reduce the struggle times ( P < 0.01) of tail-hanging test of tail-hanging test, also reduce the contents of CRH, ACTH and CORT (P < 0.01, P < 0.05).
CONCLUSIONThe four kinds of traditional Chinese herbal compound can increase the ability of anti stress by affect the different taches of the hypothalamus-pituitary-adrenal gland axle and change the stress behaviors, which are based on their respective functions of regulating Qi, dispersing phlegm, tonifying Qi and warming Yang.
Adrenocorticotropic Hormone ; blood ; Animals ; Behavior, Animal ; drug effects ; Corticosterone ; blood ; Corticotropin-Releasing Hormone ; metabolism ; Drug Combinations ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; Female ; Hypothalamo-Hypophyseal System ; drug effects ; Hypothalamus ; metabolism ; Male ; Materia Medica ; isolation & purification ; pharmacology ; Medicine, Chinese Traditional ; Ostreidae ; chemistry ; Plants, Medicinal ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Stress, Physiological ; physiopathology
10.Effect of wolfberry fruit and epimedium on DNA synthesis of the aging-youth 2BS fusion cells.
Bai-yan WU ; Jun-hua ZOU ; Shu-cong MENG
Chinese Journal of Integrated Traditional and Western Medicine 2003;23(12):926-928
OBJECTIVETo study the effect of water extracts of Wolfberry fruit (WB) and Epimedium (EM) on DNA synthesis of the aging-youth 2BS fusion cells.
METHODSHuman embryonic lung diploid fibroblasts 2BS national standard strain, were used as an aging model. Cell denucleation and cell fusion techniques were applied to observe the effect of WB and EM on DNA synthesis of 2BS fusion cells.
RESULTSIn the 0.025 (V/V) WB or EM water extract containing media, 2BS cells could be continuously cultured for 61.0 +/- 2.9 passages and 56.0 +/- 2.6 passages respectively, while in the control group it was only 49.0 +/- 2.6 passages (P < 0.01). After treatment with WB and EM separately for 2 hrs, the aging 2BS cells were denucleated and fused with young 2BS cells. The [3H]TdR incorporation percentage in these treated cells was significantly higher than that in the untreated control cells (P < 0.01).
CONCLUSIONBoth WB and EM can accelerate the DNA synthesis rate of the aging youth 2BS fusion cells and prolong the life span of 2BS cells.
Cell Fusion ; Cellular Senescence ; drug effects ; DNA ; biosynthesis ; Diploidy ; Drugs, Chinese Herbal ; pharmacology ; Embryo, Mammalian ; Fibroblasts ; cytology ; Humans ; Lung ; cytology ; Lycium