Acute Disease ; Adolescent ; Humans ; Male ; Myositis

Objective: To analyse the acuracy of color doppler ultrasound examination in the measure- ments of blood vessels in tissue flaps. Methods: 110 pieces of associated blood vessel in tissue flaps in 64 cases were measured with color doppler ultrasound examination before operation. The quality of blood vessel, its diameter, blood stream filling, the vascular pedicle length and its depth were measured. The results were checked and compared with the findings in operation. Results: Of all 110 pieces of associated blood vessel evaluated by doppler, the accurate ratio confirmed by the finding in operation was 100%. The inner diameter of 38 pieces of blood vessel measured in operation was coincident with that measured by doppler before operation, or with little difference. Conclusion: The color doppler ultrasound examination is a reliable method for evaluating the blood vessel both of the donor and host areas in maxillofacial reconstruction surgery with tissue flap.

Objective:To evaluate 2D ultrasonography(2DUS), color Doppler flowing imaging(CDFI) and color Doppler energy (CDE) in the differential diagnosis of benign and malignant tumors of salivary glands.Methods:According to the characters of 2DUS and CDFI examination, benign and malignant tumors in 142 cases of salivary glands were distinguished. The results of ultrasound examination and postoperative biopsy were checked and compared. Meanwhile the same tumor's blood flow information and hemodynamics index were compared. Results:In 2DUS examination, the inter echo and post echo of benign and malignant were different. In CDFI examination, the blood flow form could be displayed clearly. Besides Warthin tumors, the blood supply ranking of benign tumors was usually levelⅠor Ⅱ-(78.7%), and that of malignant tumors was levelⅡ+ or Ⅲ(80.0%). The artery's peak systolic velocity(PSV) of malignant tumors was higher than that of benign tumors. The rates(%) of sensitivity, specificity,accuracy,false negative, false positive, positive predicting and negative predicting of 2DUS were 70.2,87.4,81.7,29.8,12.6,73.3 and 85.6,those of CDFI 80.9,65.2,70.4,19.1,34.7,53.0 and 87.3,those of the integrated usage of ultrasonic methods 89.3,90.5,90.1,10.6,9.5,82.4 and 94.5 respectively.Conclusion:With the integrated usage of ultrasonic methods, higher accuracy of differential diagnosis for benign and malignant salivary glands tumors may be obtained.

Objective To investigate the transactivating effect of hepatitis C virus(HCV)core protein on inducible nitric oxide synthase(iNOS)gene promoter and the molecular biological mecha- nisms of HCV pathogenesis.Methods Polymerase chain reaction(PCR)technique was employed to amplify the sequence of iNOS promoter by using HepG2 genomic DNA as template,and the product was cloned into pGEM-T vector.The iNOSp gene was cut from T-iNOSp by KpnⅠand XhoⅠ,and then was cloned into pCAT3-Basic,the constructed vector was named as pCAT3-iNOSp,pCAT3-iN- OSp was transfected into the LO_2 cell line.LO_2 cell was also cotransfected with pcDNA3.1(-)-core and pCAT3-iNOSp by FuGENE 6 transfection reagents.The LO_2 cells transfected with pCAT3-Basic was used as negative control.The activity of CAT in LO_2 cells was detected by an ELISA kit after 48 hours,which reflected the transactivating function of HCV core protein to iNOS gene promoter.Re- sults The expressive vector pcDNA3.1(-)-core and report vector pCAT3-iNOSp had been construc- ted and confirmed by restriction enzyme digestion and sequencing.The expression of CAT in LO_2 cells transfected with pCAT3-iNOSp and peDNA3,1(-)-core was 11 times as higher as that of pCAT3-bas- ic,and 6 times as higher as that of pCAT3-iNOSp.Conclusion It is suggested that HCV core protein can transactivate iNOS gene promoter.

Adolescent ; Adult ; Allyl Compounds ; poisoning ; Chronic Disease ; Female ; Humans ; Occupational Diseases ; diagnosis ; therapy ; Poisoning ; diagnosis ; therapy

Cell Cycle Checkpoints ; drug effects ; Cell Line, Tumor ; Gene Expression Regulation ; drug effects ; Humans ; NF-kappa B ; metabolism ; Scorpion Venoms ; pharmacology

Objective To investigate the survival rate and the influencing factors in lupus nephritis (LN) patients with neuropsychiatric systemic lupus erythematosus (NPSLE). Methods Clinical characteristics and biochemical markers of 78 patients including 59 variances were analyzed. Patients were followed up from the onset of NPSLE to death. Patient survival rate was estimated by Kaplan-Meier method. Cox regression model was used to analyze influencing factors. Results Sixteen (20.5%) of 78 patients died of SLE or its complications. Infection was the main cause of death (31.3%). One-, 3-, 5- and 10-year survival rates were 83.2%, 81.7%, 76.7% and 76.7%, respectively. Hypertension (RR =6.965,95% CI:1.578-30.746, P= 0.010), pulmonary infection (RR=8.171,95% CI:1.954-34.177, P=0.004)and acute renal failure (RR=6.978,95%CI:2.063-23.609, P=0.002) were risk factors of mortality, while cyclophosphamide (CTX) impulse therapy (RR =0.130,95 % CI:0.031-0.541, P=0.005) and resolution of NPSLE (RR= 0.169, 95%CI:0.042-0.679,P=O.012)were protective factors. Conclusions Infection is the main cause of death in patients of LN complicated with NPSLE. Survival rate of LN patients with NPSLE in this study is lower than those of LN and NPSLE alone reported by other authors. Hypertension, pulmonary infection and acute renal failure are risk factors of mortality, while CTX impulse therapy and resolution of NPSLE reduce the mortality and improve the prognosis.

Objective To investigate the feasibility of laparoscopic surgery for hydronephrosis caused by retroper-itoneal fibrosis.Methods Reported the diagnosis and treatment procedure and result of 1 case of hydronephrosis caused by retroperitoneal fibrosis on left side,who underwent laparoscopie surgery.Investigated the feasibility of hydronephrosis caused by retroperitoneal fibrosis with literature review.A 55-year-old female patient had interrupt-able left lower extremity swollen with nausea and vomiting for five months.The blood test showed progressive eleva-ting of creatinine and urea nitrogen.The highest creatinine value was 503 μmol/L.The computerized tomography showed renal atrophy on right side and there was a stenosis on crossing place between inferior segment on left ureter and iliac blood vessels,also there is a soft tissue image in front of aorta and around iliac blood vessels,the superior ureter was dilated.We set double J tube into left ureter before surgery with semi-reclining position toward right side,set trocar cannula through 1 era,4 em below the umbilicus as well as the same two points on lateral external oblique muscle of abdomen,set laparoscope from 1 cm below the umbilicus.We found the abdominal membrane pale and touch as tabular.There was serious eonglutination between middle segment ureter and around iliac blood vessels after we cut open retroperitoneal membrane.We carefully dissected the ureter for 9 cm and soluted the eonglutination a-round the ureter,then set the ureter into abdominal cavity and closed retroperitoneal membrane.Results The pa-tient recover well after surgery and sutured out after 5 days,pulled out the double J tube after I month.The patient had low back pain ,fever and oliguria after the double J tube was pulled out and recovered with spasmolysis,analge-sia and antibiosis treatment after 2 days.The voiding volume and renal function became normal.The B-type ultra-sound test showed hydronephrosis on left side was lessened.The ultrasound test after 3 months result showed hydro-nephrosis was significantly lessened than before surgery and renal function was normal.Conclusions The laparo-scopic ureterolysis surgery for retroperitoneal fibrosis is minimally invasive,less suffering and quick recovery.Be-cause of few case report and short follow-up time is more eases and long-term follow-up are needed to elucidate the therapeutic efficacy of this treatment.

Adolescent ; Adult ; Ankylosis ; complications ; surgery ; Female ; Humans ; Imaging, Three-Dimensional ; Male ; Micrognathism ; complications ; surgery ; Models, Anatomic ; Skull ; anatomy & histology ; Temporomandibular Joint Disorders ; complications ; surgery ; Young Adult

Animals ; Flunarizine ; pharmacology ; Hippocampus ; drug effects ; physiopathology ; Male ; Neurons ; drug effects ; physiology ; Penicillins ; adverse effects ; Rats ; Rats, Wistar ; Seizures ; chemically induced ; physiopathology