1.Effect of β-cyclodextrin inclusion complex on transport of major components of Xiangfu Siwu decoction essential oil in Caco-2 cell monolayer model.
Jun-zuan XI ; Da-wei QIAN ; Jin-ao DUAN ; Pei LIU ; Yue ZHU ; Zhen-hua ZHU ; Li ZHANG
China Journal of Chinese Materia Medica 2015;40(15):2970-2974
Although the essential oil of Xiangfu Siwu decoction (XFSWD) has strong pharmacological activity, its special physical and chemical properties restrict the clinical application and curative effect. In this paper, Xiangfu Siwu decoction essential oil (XFS-WO) was prepared by forming inclusion complex with β-cyclodextrin (β-CD). The present study is to investigate the effect of β-CD inclusion complex on the transport of major components of XFSWO using Caco-2 cell monolayer model, thus to research the effect of this formation on the absorption of drugs with low solubility and high permeability, which belong to class 2 in biopharmaceutics classification system. A sensitive and rapid UPLC-MS/MS method was developed for simultaneous quantification of senkyunolide A, 3-n-butylphthalide, Z-ligustilide, dehydrocostus lactone and α-cyperone, which are active compounds in XFSWO. The transport parameters were analyzed and compared in free oil and its β-CD inclusion complex. The result revealed that the formation of XFSWO/β-CD inclusion complex has significantly increased the transportation and absorption of major active ingredients than free oil. Accordingly, it can be speculated that cyclodextrin inclusion complex can improve bioavailability of poorly water-soluble drugs. Above all these mentioned researches, it provided foundation and basis for physiological disposition and pharmaceutical study of XFSWD.
Biological Transport
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Caco-2 Cells
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Drugs, Chinese Herbal
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analysis
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Humans
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Oils, Volatile
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analysis
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beta-Cyclodextrins
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pharmacology
2.Quality control of Angelica sinensis with standard reference extract.
Jun-Zuan XI ; Yi-Yun QIAN ; Jin-Ao DUAN ; Hui YAN ; Yu-Yang ZHAO ; Jie ZHANG ; Ling GUO ; Da-Wei QIAN
China Journal of Chinese Materia Medica 2014;39(19):3803-3807
To improve the quality standard of Angelica sinensis, solve the problem of lacking relevant reference substance, a new method-based on the standard reference extract (SRE) was applied to achieve the quality control of Angelica sinensis. SRE of Angelica sinensis was obtained by chromatographic separation technology. After calibration of three makers of the SRE, an UPLC analytical method was developed to determinate the contents of the makers. T-test was used for comparison of the determination results of two methods (reference substances and SRE as reference, respectively), and the results demonstrated that there is no significant difference between the two methods. The presented method is very convenient and practical, which can be used for the quality control of Angelica sinensis.
Angelica sinensis
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chemistry
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Calibration
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Chromatography, High Pressure Liquid
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standards
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Drugs, Chinese Herbal
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chemistry
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standards
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Quality Control
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Reference Standards