Objective To investigate the characteristic changes of auditory brainstem response (ABR) of nor‐mal hearing infants .Methods A total of 212 cases (327 ears) normal hearing infants were enrolled for ABR test . According to age ,167 ears was in 1~3 months age group ,105 ears was in 3~6 months age group ,55 ears was in 6~12 months age group .Each group consisted of ABR date were statistically analyzed .Results For 75 dB nHL click sound stimulus ,the peak latency and interpeak latency of Ⅲ ,Ⅴ ,Ⅰ — Ⅲ ,Ⅲ — Ⅴ ,Ⅰ — Ⅴwaves of normal hearing infants progressively shortened as they grew older except the wave Ⅰ .The ABR thresholds were in significantly difference among the groups .The amplitude ratio of wave V/I increased with the grewing age .Each group of data showed statistically significant differences .When the stimulus intensity reached at thresholds ,the occurrence rate of wave I ,III and V were different as they grew older .Conclusion Infants with auditory nervous system was in con‐stant development and improvement ,all parts of the auditory pathway of neural development was not balanced .

Probiotics are a class of colonization in the intestine to maintain a balanced intestinal flora and stimulate the mucosal immune tissue,have a major impact on the intestinal mucosal immune beneficial microbial communities.Neonatal development due to their own reasons,have low immune function,intestinal function is not sound,fewer bacterial species and so on.In this paper,probiotics on neonatal intestinal immune regulation,probiotics and neonatal necrotizing enterocolitis,feeding intolerance in premature children,newborn diarrhea,neonatal jaundice,allergic diseases,the safety of probiotics will be reviewed,including the clinical application and research progress of probiotics in neonates.Designed to enable the general pediatrician a better understanding and application of probiotics,so that such drugs more rational clinical application.

Aged ; Catheter Ablation ; adverse effects ; Echocardiography ; Heart Atria ; Humans ; Male ; Postoperative Complications ; diagnostic imaging ; Tachycardia, Supraventricular ; diagnostic imaging ; surgery ; Thrombosis ; etiology

BACKGROUND:In the repair of urinary tract defects, we have been actively trying to construct the urinary tract substitutes with normal physiological function through combining ideal seed cel s and proper scaffold materials by tissue engineering method. OBJECTIVE:To investigate the biocompatibility of bone marrow mesenchymal stem cel s with rabbit bladder acel ular matrix scaffold. METHODS:Rabbit bone marrow mesenchymal stem cel s were isolated and cultured using density gradient centrifugation method. Passage 3 rabbit bone marrow mesenchymal stem cel s were cultured on the rabbit bladder acel ular matrix. The cel s were counted every day for 12 days, to drawn a cel growth curve. Bone marrow mesenchymal stem cel s cultured alone were used as control group. RESULTS AND CONCLUSION:Bone marrow mesenchymal stem cel s were successful y seeded onto the bladder acel ular matrix. Under the inverted microscope, the cel s grew out of the bladder acel ular matrix, and a great amount of long spindle-shaped cel s were found around the bladder acel ular matrix. With 5 days of inoculation, the cel s in the two groups grew gently;at 6-9 days, the cel growth curve gradual y became steeper, and the cel division and growth were increased exponential y;at 10-12 days, the cel s recovered to a gentle state. Cel growth curves in the two groups were basical y coincident, suggesting that rabbit bone marrow mesenchymal stem cel s have good biocompatibility with the bladder matrix.

Objective To seek the factors affecting distortion product otoac oustic emisson in newborn hearing screening. Methods All newborns in our hospital received hearing sc reening by DPOAE and wer e investigated for the factors such as: birth history, pregnancy history, family history, the social position of the family. All data were analysed by SPSS. Results 82.8%(3 242/3 944) newborns passed the hearing sc reening. Pass rates were h igher in right ears, female baby, monocyesis, normal or high birth weight and fo rceps delivery. The Results of the first hearing screening were correlated with birth weight,sex and fetus number by logistic regression. Conclusion The factors suc h as sex, ways of labor, fetus number, fetus age, and birth weight can affect t he results of first hearing screening significantly.

Graft vs Host Disease ; drug therapy ; Humans ; Tumor Necrosis Factor-alpha ; antagonists & inhibitors ; therapeutic use

Objective To investigate the effect of different sedation depth of anesthesia the BIS settings (BISis 45 and 55) to perioperative hemodynamic. Methods Thirty-two patients were randomly divided into two groups. Through closed-loop target controlled infusion technology, depth of sedation anesthesia in A:the BIS=45, and in B: the BIS=55.12 time points(i.e. before operation, tracheal intubation instant, operation beginning, 20, 40,60,80,100 and 120 minutes after operation beginning, operation accomplishment point, tracheal catheter pulling out point, before patient′s carried out of operation room)perioperativenoninvasivemean arterial blood pressureandheart rate wererecorded. Results Themean arterial blood pressurebetween the two groups at time point T2,T11 was significantly different. Within group B,compared with T1, there wassignificantdifferenceat T2,T11. Heart ratein group B at T5and T10increasedsignificantly compared with A.Within group B,heart rate at T5,T6 increased significantly compared with that at T1. Conclusion Compared with BIS 55, the sedation anesthesia depth of BIS 45 can better keep stable of blood pressureandheart rate.

Objective To observe changes of motilin(MTL) levels in gastric body, duodenum and plasma in rat model of acute incisional pain.Methods A total of 156 healthy male adult SD rats,weighing 180-220 g,were randomized into two groups:control group (group C,n=78) and incisional pain group (group P,n=78),Rats in P group received incision on the right plantaris. Values of paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL) at dif?ferent time points of 24 hours before operation (T0) and 1 hour (T1),6 hours (T2),24 hours(T3),48 hours (T4) and 72 hours (T5) after operation were measured in six rats chosen randomly from each group. Twelve rats were chosen from each group at T 0-5, and sacrificed. The MTL levels in plasma, the mucosal tissues of gastric body and duodenum were detected by ELISA. Re?sults Compared with group C, PWMT and PWTL were significantly decreased at T1-4 in group P. The MTL levels were sig?nificantly decreased in plasma and gastric body (P<0.05).The MTL level was significantly increased at T1-4 in duodenum (P<0.05),and no significant changes were found at T0 and T5 in P group(P>0.05). The plasma MTL levels were positively correlated with PWMT and PWTL (r=0.952,r=0.879,respectively,P<0.01) in P group. The MTL levels in gastric body were positively correlated with PWMT and PWTL(r=0.970,r=0.931,respectively,P<0.01) in P group. The MTL levels were neg?atively correlated with PWMT and PWTL(r=-0.991,r=-0.975,respectively,P<0.01) in duodenum in P group. Conclu?sion The MTL levels in plasma and gastric body are decreased in rat model of acute incisional pain, and increased in duo?denum.

Objective To investigate the clinical efficacy of locking compression plate fixation through a modified anterolateral approach for posterolateral tibial plateau fractures.Methods From June 2010 to March 2012,19 patients with posterolateral tibial plateau fractures underwent locking compression plate fixation through a modified anterolateral approach in our hospital.There were 11 males and 8 females,aged from 26 to 55 years (average,38.3 years).The injury causes included traffic accident in 10 cases,fall from height injury in 7 cases and falling injury in 2 cases.Two patients had avulsion fracture of the anterior cruciate ligament.The modified lateral S-shaped incision was adopted for all patients.All the patients underwent early and suitable rehabilitation after operation.The radiographic and clinical results were evaluated by using X-rays and the Rasmussen score,respectively.Results The average operative time was 95 minutes (range,80 to 120 minutes),and the average intraoperative blood loss was 180 ml (range,100 to 400 mi).All the patients were followed up for 12 to 24 months (average,16.2 months).Bone union was obtained in all patients,and the bony union time ranged from 8 to 14 weeks.There was no implant loosening/ breakage,bone nonunion,genu valgum,genu varum,redisplacement of fracture,and knee instability.Anatomic reduction was obtained in 18 patients.For one patient with posterolateral comminuted dislocation fracture,CT scan showed a step-off of 2 mm in joint surface after operation,and at final follow-up,the patient suffered from mild pain and the range of motion of the knee joint was 0 to 105 degree.The range of motion of the knee joint was 5 to 90 degree in one patient.The Rasmussen score ranged from 13 to 30 (average,22.9±4.9); the results were excellent in 10 cases,good in 7 cases and fair in 2 cases,and the excellent and good rate was 89.5％.Conclusion Locking compression plate fixation through a modified anterolateral approach is an effective method for posterolateral tibial plateau fractures,which has several advantages,such as simple and safe operation,stable fixation and less complications.