Biomimetics ; Blood Cells ; metabolism ; CD55 Antigens ; blood ; CD59 Antigens ; blood ; Cell Membrane ; Humans ; Male ; Submarine Medicine

Food Contamination ; prevention & control ; Public Health Practice

Maximum Allowable Concentration ; Mineral Fibers ; analysis ; Occupational Exposure ; analysis

Emergencies ; Food Services ; organization & administration ; Foodborne Diseases ; prevention & control ; Humans ; Risk Assessment

BACKGROUND: It is thought in traditional Chinese medicine that "the head is the residence of intelligence", "the head is the leader of body, and the focus of the spirit". Therefore, scalp electro-acupuncture can induce resuscitation, relieve mental confusion, dredge the meridian and eliminate the depression in treatment of post-stroke depression.OBJECTIVE: To observe the curative effect of head acupuncture on poststroke depression.DESIGN: Randomized controlled and single blind evaluationSETTING: Department of Acupuncture and Moxibustion, Nanfang Hospital of Southern Medical University; College of Acupuncture and Massage,Guangzhou University of Traditional Chinese Medicine.PARTICIPANTS: Totally 90 patients with post-stroke depression were from Affiliated Nanfang Hospital and,Zhujiang Hospital of Southern Medical University, First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine were recruited. The Score of all the patients in Hamilton Rating Scale for Depression (HRSD) were ＞ 20. Ninety patients were randomly divided as scalp acupuncture group (n=46) and routine acupuncture group (n=44).METHODS: The patients in the routine acupuncture group were treated mainly at points of three-yang meridians of foot and hand at the hemiplegia side. The points include jianyu (LI-15) , binao (LI-14) , quchi (LI-11),shousanli (LI-10), waiguan (SJ-5), hegu (LI-4), biguan (St-31), liangqiu (St-34), zusanli (St-36), yanglingquan (GB-34), kunlun (UB-60), jiexi (St41). The above points were divided into 2 groups, 1 group each day, alternately. Even reinforcing-reducing method was used, with 30-minute needle retention, 6 successive days treatment with 1 day rest, for 6 weeks totally.The patients in the scalp acupuncture were treated with, besides the above method, scalp acupuncture. Mid-parietal line, mid-frontal line, laterofrontal lineI (both sides)were chosen. After arrival of qi, G6805 type electro-acupuncture apparatus (interrupted wave, 50 Hz,4 V) was connected, with 30-minute needle retention, 6 suocessive days treatment with 1 day rest, 6 for 6 weeks totally. HRSD was used to evaluate the status of depression before and after treatment. Stroke score was used to evaluate the curative effect of stroke.MAIN OUTCOME MEASURES: HRSD scores and curative effect of apoplexy before and after treatment of the patients in two groups.RESULTS: According to the intention treatment, all 90 patients entered the result analysis. ① HRSD score after treatment was lower than that be fore treatment in the scalp acupuncture group,and that after treatment group in the routine acupuncture group (8.55±1.73,30.61±4.02,15.04±2.33,P ＜ 0.01 ). ②There was no significant difference of the total effective rate in the two groups , but some tendency indicated the effective rate in the scalp acupuncture group was better than that in the routine acupuncture group ( 100% ,98% ,x2=1.9167 ,P ＞ 0.05).CONCLUSION: Scalp electro-acupuncture has obvious curative effect on post-stroke depression. The amelioration of melancholia has a certain promoting effect on rehabilitation of apoplexy.

The effects of magnolol (Mag) on hyperglycemia and hyperlipemia, hepatic oxidative stress and cytochrome P4502E1 (CYP2E1) activity of diabetic rats induced by high-fat diet (HFD) and streptozotocin (STZ) were studied. After oral administration of Mag (25, 50 and 100 mg x kg(-1) x d(-1)) for continuous 10 weeks, the blood glucose and lipids (TC, TG and LDL-C) levels, as well as the hepatic CYP2E1 activity and MDA content of diabetic rats, decreased significantly (P < 0.05 or P < 0.01), whereas the oral glucose tolerance and hepatic antioxidant enzymatic activities (CAT and GSH-Px) of diabetic rats, increased significantly (P < 0.05 or P < 0.01). The results indicated that Mag was effective against the hepatic oxidative damage, hyperglycemia and hyperlipemia of diabetic rats induced by HFD and STZ, and the inhibition of Mag on hepatic CYP2E1 activity could be an important mechanism of Mag against hepatic insulin resistance and oxidative damage.
Animals ; Biphenyl Compounds ; isolation & purification ; pharmacology ; Blood Glucose ; metabolism ; Cholesterol ; blood ; Cholesterol, LDL ; blood ; Cytochrome P-450 CYP2E1 ; metabolism ; Diabetes Mellitus, Experimental ; blood ; drug therapy ; metabolism ; Diet, High-Fat ; Glucose Tolerance Test ; Hypoglycemic Agents ; isolation & purification ; pharmacology ; Lignans ; isolation & purification ; pharmacology ; Liver ; metabolism ; Magnolia ; chemistry ; Male ; Oxidative Stress ; drug effects ; Plants, Medicinal ; chemistry ; Protective Agents ; pharmacology ; Rats ; Rats, Wistar ; Streptozocin ; Triglycerides ; blood

To ensure the quality and safety of Panax notoginseng, a method for the simultaneous determination of 10 mycotoxins in Panax notoginseng was developed using ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). The sample was extracted with acetonitrile and purified by HLB multifunction cleanup column. The separation was performed on a Phenomenex Kinetex XB-C18 column by gradient elution using methanol and 5 mmol·L(-1) ammonium acetate as mobile phase. The targeted compounds were detected in MRM mode by mass spectrometry with electrospray ionization (ESI) source operated in both positive and negative ionization modes. The linear relationships of the 10 mycotoxins were good in their respective linear ranges. The correlation coefficients (r) ranged from 0.9981 to 1.0000. The LOQs of the 10 mycotoxins were between 0.15 and 8.6 μg·kg(-1). The average recoveries ranged from 73.8% to 107.0% with relative standard deviations (RSDs) of 0.10%-10.9%. The results demonstrated that the proposed method was sensitive and accurate, and suitable for the mycotoxins quantification in Panax notoginseng.
Chromatography, High Pressure Liquid ; Chromatography, Liquid ; Drug Contamination ; Mycotoxins ; analysis ; Panax notoginseng ; chemistry ; Tandem Mass Spectrometry

Borneol is a traditional Chinese medicine. In the past few years, many studies showed that borneol can improve the bioavailability of other drugs, promoting drugs to cross the blood-brain barrier, so the potential drug interactions between borneol and other medicines have attracted great attention, but the influence of borneol to CYP450 and its isoforms are rarely reported. In this research, male Wistar rats were orally administered by borneol for 7 days, then the mRNA and protein expression and the activities of CYP2D were detected, we also compared the pharmacokinetic parameters of CYP2D's specific substrate between control group and borneol group. The results show that borneol (33, 100 and 300 mg x kg(-1) x d(-1)) have no significant effect on CYP2D, while the activites of CYP2D increased 1.71, 1.97 and 2.89 times comparing to the control group. At the same time, borneol (300 mg x kg(-1) x d(-1)) caused the C(max) decreased 10.6% (P > 0.05), AUC(0-∞) decreased 27.5% (P < 0.01), CL/F increased 41.1% (P < 0.01), V(z)/F increased 23.1% (P > 0.05) of dextromethorphan. Our data provided that borneol speed up dextromethorphan's elimination in vivo. Since the activity of CYP2D can be induced by borneol, the metabolic interactions might happen when borneol and the substrate drug CYP2D are used together.
Animals ; Aryl Hydrocarbon Hydroxylases ; metabolism ; Blood-Brain Barrier ; Bornanes ; pharmacology ; Cytochrome P-450 Enzyme Inducers ; pharmacology ; Dextromethorphan ; Drug Interactions ; Liver ; drug effects ; enzymology ; Male ; Medicine, Chinese Traditional ; RNA, Messenger ; Rats ; Rats, Wistar

Asbestos ; adverse effects ; chemistry ; classification ; Carcinogens ; Fibrosis ; Humans ; Occupational Exposure

Acquired Immunodeficiency Syndrome ; blood ; pathology ; physiopathology ; Female ; Humans ; Infant