1. Synthesis of novel matrine derivatives and their in vitro anti-inflammatory activity
Academic Journal of Second Military Medical University 2011;32(11):1223-1226
Objective To design and synthesize a series of novel matrine derivatives and to investigate their in vitro ant-inflammation activities. Methods Ten novel matrine analogues were synthesized via thiosulfate reaction and classical Michael addition using sophocarprne as the staring material. The effects of all the analogues on TNF-α production and NF-κB transcriptional activity were evaluated in vitro. Results The synthesied compounds were confirmed correct by 1HNMR and ESI-MS. Biological studies showed that the synthetic derivatives had inhibitory effects against TNF-α production and NF-κB transcriptional activity. Compound If had the strongest inhibitory effect against TNF-α production, with an IC50 value of 9. 4 μmol/L. Conclucion Introduction of finer small substitutes at the 13-potion of matrine can enhance its ant-inflammatory activity.
2.Calcium-binding protein secretagogin is a novel neuroendocrine marker.
Wen-jun WANG ; Fan ZHANG ; Guo-xiang XU ; Chen CHEN
Chinese Journal of Pathology 2010;39(9):627-628
Adenoma
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metabolism
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Adrenal Cortex Neoplasms
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metabolism
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Biomarkers, Tumor
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metabolism
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Calcium-Binding Proteins
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metabolism
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Carcinoma, Neuroendocrine
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metabolism
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Humans
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Lung Neoplasms
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metabolism
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Neuroendocrine Cells
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metabolism
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Neuroendocrine Tumors
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metabolism
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Secretagogins
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Small Cell Lung Carcinoma
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metabolism
3.Comparison of complete-block radical gastrectomy and traditional radical gastrectomy
Fenghua GUO ; Fen LUO ; Xiang MAO ; Jun WANG ; Zhiming WANG
Fudan University Journal of Medical Sciences 2010;37(1):103-105,123
Objective To introduce the techniques and specification of complete-block radical gastrectomy, and to compare with traditional radical gastrectomy in the operative time, surgical costs and postoperative complications. Methods Thirty gastric cancer patients meet the criteria were randomly divided into 2 groups. Group A: complete-block radical gastrectomy;group B: radical gastrectomy. Analysis and comparison were made on tumor site, time consuming of block, the proportion of gastrointestinal reconstruction, operative time, cost of block, pathological TNM stage, length of stay, postoperative complications and postoperative chemotherapy. Results Group A has longer operative time than group B (P<0.05). However, there was no significant statistical difference in the other indicators between these 2 groups. Conclusions Complete-block technology can separate cancer more completely during operation with the advantage of simplicity, low-cost, little effect on the subsequent operation. The incidence of postoperative complications and length of stay with the traditional surgery there was no significant difference.
4.Progress in research on mechanism of pathogenesis and treatment of acute paraquat poisoning.
Yan-jun RUAN ; Xiang-dong JIAN ; Guang-ran GUO
Chinese Journal of Industrial Hygiene and Occupational Diseases 2009;27(2):114-116
Herbicides
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poisoning
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Humans
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Paraquat
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poisoning
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Poisoning
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metabolism
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pathology
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therapy
6.Studies on the Central Regulatory Mechanism of Acupuncture-moxibustion Treatment for Gastric Mucosal Injury
Juan XIANG ; Guo CHEN ; Fei LI ; Liting XIANG ; Ying CHEN ; Jun YU ; Zhou YANG ; Liang PENG
Shanghai Journal of Acupuncture and Moxibustion 2016;35(12):1484-1487
This article provides the possible mechanism of acupuncture-moxibustion repair of gastric mucosal injury from central regulation and puts it forward that the nucleus of solitary tract is the primary regulation center for the injury repair and has the effect of collecting and integrating information. In addition, it is put forward that neural regulation is a main regulatory mechanism for the injury repair and besides, endocrine, immune and humoral regulations participates in the modulation and gastric mucosal repair involves a composite regulatory mechanism in which the central nervous system, neuroendocrine-immune network and neurohumoral regulation take part.
7.The effect of scalp acupuncture combined with rehabilitation training on the regulation of neuro-plasticity-associated protein MAP-2 in gerbils modelling cerebral ischemia and reperfusion
Xiaode GUO ; Ying ZHOU ; Rui WANG ; Xiang ZHOU ; Yinfeng GUO ; Jun TANG ; Shan GAO
Chinese Journal of Physical Medicine and Rehabilitation 2015;37(1):7-10
Objective To observe the effect of scalp acupuncture and rehabilitation training on the regulation of neuro-plasticity-associated protein MAP-2 in gerbils modelling cerebral ischemia and reperfusion.Methods Cerebral ischemia and reperfusion were modelled into thirty-six male gerbils.They were then randomly divided into a model group (A),a scalp acupuncture and rehabilitation training group (B) and a rehabilitation training group (C),each of 12 cases.The scalp acupuncture and rehabilitation training were administered daily to the gerbils in group B beginning 24 h after modeling,for 14 d.Any changes in Bederson scores were observed after one,7 and 14 days.On the 14th day the expression of MAP-2 around the infarct focus was detected using western blotting in all 3 groups.Results On the 7th day the average Bederson score in group B (1.81 ± 0.52) was not significantly different from that in group A (2.13 ± 0.49) or group C (2.00 ± 0.31) (P > 0.05).At the end of the treatment,however,there were significant differences between the groups,with group B (0.47 ± 0.31) scoring significantly better than groups C (1.04 ± 0.63) and A (1.46 ± 0.72) (P < 0.05).Group C was also significantly better than group A (P <0.05).The expression of MAP-2 as measured as integrated optical intensity (IOD) in group B (0.91 ±0.18) was significantly higher than that in group A (0.43 ± 0.21) and group C (0.67 ± 0.24) (P < 0.05) ; so was group C compared to group A (P <0.05).Conclusion The scalp acupuncture combined with rehabilitation training can improve the recovery of motor function and enhance the expression of neuro-plasticity-associated protein MAP-2 in gerbils with after cerebral ischemia and reperfusion.
8.Clinical and pathological study on the correlation between serum prostate specific antigen and benign prostatic hyperplasia
Pei-Jun LI ; Xiang-Hua ZHANG ; Li-Jun GUO ; Yan-Qun NA ;
Chinese Journal of Urology 2001;0(06):-
Objective To evaluate the clinical and pathological factors that may influence serum prostate specific antigen(PSA)level in patients with benign prostatic hyperplasia(BPH).Methods A total of 561 cases of BPH diagnosed by pathological examination following operation were analyzed retrospec- tively.The patients'mean age was(68.3?6.3)years.The International Prostate Symptom Score(IPSS)was 21.1?7.4;the quality of life(QOL)was 4.5?0.8;Qmax was(7.3?3.3)ml/s;prostate volume(PV) was(69.8?36.8)ml;andpost-void residue(PVR)was(81.9?105.8)ml.Results Of 561 cases,223 cases(39.8%)had serum PSA level at 4-10 ng/ml,and 91 cases(16.2%),over 10 ng/ml.The level of serum PSA was not significantly associated with age,IPSS,QOL,Qmax and PVR(r=0.08,0.03,0.06,0.04 and 0.09,respectively;P>0.05),but significantly associated with prostate volume of BPH(r=0.42,P<0.01).The level of serum PSA was significantly elevated in BPH patients with enlarged prostate volume (F=93.45,P<0.05)and the history of acute urinary retention(x~2= 59.1,P<0.01).Additionally,a significantly increased PSA level was noted in tissue specimens with glandular hyperplasia(x~2=16.14,P<0.01)or necrotic loci in BPH patients(x~2=36.06,P<0.01).Conclusions An elevated serum PSA level was observed in more than 50% of BPH patients undergoing surgical resection.Enlarged prostate volume,the history of acute urinary,retention,and tissue specimens with glandular hyperplasia or necrotic foci are considered to be the main causes leading to elevated PSA level in BPH patients.
9.Agrobacterium tumefaciens-mediated transformation of Aureobasidium pullulans and high-efficient screening for polymalic acid producing strain.
Guangwei TU ; Yongkang WANG ; Jun FENG ; Xiaorong LI ; Meijin GUO ; Xiang ZOU
Chinese Journal of Biotechnology 2015;31(7):1063-1072
To develop a genetic transformation method of Aureobasidium pullulans and T-DNA insertion for high-efficient screening of polymalic acid (PMA) producing strain. Agrobacterium tumefaciens-AGL1, containing the selection genes encoding hygromycin B phosphotase or phosphinothricin acetyltranferase, was used to transform Aureobasidium pullulans CCTCC M2012223 and transformants were confirmed by colony PCR method. Transferred DNA (T-DNA) insertional mutants were cultured in microwell plate, and screened for high-titer PMA producing strain according to the pH response model. DNA walking was used to detect the insertion sites in the mutant. Results show that the selection markers could stably generated in the transformants, and 80 to 120 transformants could be found per 10(7) single cells. A high-titer PMA mutant H27 was obtained, giving a good PMA production caused by the disruption of phosphoglycerate mutase, that increased by 24.5% compared with the control. Agrobacterium tumefaciens-mediated transformation and high-efficient screening method were successfully developed, which will be helpful for genetic transformation of Aureobasidium pullulans and its functional genes discovery.
Agrobacterium tumefaciens
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Ascomycota
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genetics
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metabolism
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DNA, Bacterial
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Malates
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metabolism
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Polymerase Chain Reaction
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Polymers
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metabolism
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Transformation, Genetic