1.Research Progress on Photocatalytic Degradation of Methyl Tert-butyl Ether in Water
Shigui SUI ; Jun QIN ; Xingpeng HE
Journal of Environment and Health 1993;0(01):-
The methyl tertiary butyl ether(MTBE),as an addictive in gasoline,is used widely since the leaded gasoline has been prohibited.By some ways,MTBE can be transferred into water.Since the degradation of MTBE is very slow naturally,the pollution of MTBE in water is attracting more and more attentions.How to remove the MTBE economically and effectively has become a hot spot in common concern.In this paper,the species of photocatalytic degradation technique and the methods were reviewed including UV-Fenton,UV-H2O2,UV-O3,UV-TiO2 and visible-light photocatalytic decomposition with TiO2 were used to degradation of MTBE in water.
2.Effect of apoptosis inducing factor on promoting apoptosis of hypertrophic cardiomyocytes induced by hypoxia/reoxygenation
Xiaobo ZHOU ; Zuoyun HE ; Xuejun YU ; Jun QIN
Medical Journal of Chinese People's Liberation Army 1982;0(03):-
Objective To evaluate the effect and significance of apoptosis inducing factor (AIF) on promoting apoptosis of hypertrophic cardiomyocytes induced by hypoxia/reoxygenation. Methods Cardiomyocytes of neonatal Kunming strain rats were isolated and cultured. Cardiomyocyte hypertrophy was induced by angiotensinⅡ (0.1?mol/L for 12h), and the cells were cultured under the condition of hypoxia and reoxygenation. The cultured cardiomyocytes were then divided into seven groups: hypertrophic cardiomyocyte control (HC) group, hypoxia for 8h (H8h) group, hypoxia for 12h (H12h) group, hypoxia for 8h and reperfusion for 4h (H8h/R) group, hypoxia for 12h and reperfusion for 4h (H12h/R) group, hypoxia for 12h+siRNA transfection (H12h+siRNA) group, and hypoxia for 12h and reperfusion for 4h+siRNA transfection (H12h/R+siRNA) group. Cardiomyocytes were transfected with AIF siRNA. Apoptosis was detected with Hoechst 33258 staining. The AIF mRNA and protein expressions were detected by RT-PCR and Western blotting. Results The levels of AIF mRNA and protein expression were significantly higher in H8h group and H12h group (mRNA: 0.52?0.04, 0.85?0.10, respectively; protein: 2.07?0.15, 3.12?0.19, respectively) compared with that in HC group (mRNA: 0.29?0.08; protein: 1.00?0.04, P0.05). The percentage of apoptosis in H12h/R+siRNA group (24.90%?3.90%) was significant higher than that in H12h/R group (14.50%?1.32%, P
4.Real world analysis of traditional cardiovascular risk factors in 6040 patients with suspected coronary heart disease undergoing angiography
Lisheng JIANG ; Qin SHAO ; Jun BU ; Ben HE
Chinese Journal of Interventional Cardiology 2016;24(9):497-501
Objective To analyze the real world status of traditional known cardiovascular risk factors in patients with coronary heart disease ( CHD ) .Methods 6040 in-hospital patients with CHD or suspected CHD undergoing angiography from 01/01/2013 to 02/28/2015 were retrospectively analyzed . According to angiography result , patients with severe coronary artery lesion and undergoing percutaneous coronary intervention (PCI) were enrolled in the PCI group (n=2808) and patients without severe coronary artery lesion and not undergoing PCI or CABG were enrolled in the No-PCI/CABG group (n=3232).Patients in the PCI group were further divided into 3 subgroups which were STEMI group , NSTEMI/UA group and stable angina (SA) group.Results (1) Compared with the No-PCI/CABG group, patients in the PCI group have higher ratio of male patients (75.4% vs.53.1%, P<0.0001), older average age (64.83 ±0.20 vs. 63.39 ±0.18 years old , P <0.0001 ) , and higher existing rates of traditional risk factors including hypertension (66.7%vs.54.7%, P<0.0001 ) , diabetes/impaired glucose tolerance ( IGT ) ( 37.0% vs. 20.8%, P<0.0001), stroke(7.0%vs.5.4%,P=0.0098)and chronic kidney disease (CKD) (4.3%vs. 2.8%, P=0.001 ) , but there was no statistic difference in existing rates of dyslipidemia between the two groups.(2)In the PCI group,female patients had higher prevalence of hypertension (74.1%vs.64.3%, P<0.001), diabetes/IGT (42.5%vs.35.3%, P=0.0007) and stroke (9.4%vs.6.2%, P=0.0054) than the male patients.There were no significant sex difference in these comorbidities as above in No-PCI/CABG group.Female patients had higher prevalence of dyslipidemia than male patients in both PCI and No -PCI/CABG groups.(3) Among all the 3 PCI subgroups, STEMI patients presented with youngest average age (62.54 ±0.45 vs.65.15 ±0.28 vs.66.17 ±0.34 years old, P<0.0001) and highest male patient ratio (83.9%vs.72.9% vs.72.3%, P<0.0001).Patients in the SA subgroup had the highest prevalence of hypertension and prior revascularization including PCI and CABG .Patients in the NSTEMI/UA subgroup had the highest rates of diabetes/IGT.No significant differences were observed in the prevalence of dyslipidemia , CKD and stroke among all the subgroups .Conclusions Hypertension and diabetes are the leading risk factors of coronary artery disease , and prior revascularization is also an important cause of stable angina and NSTEMI /UA undergoing PCI.Patients requiring PCI were found to be more of male gendor , but female patients has higher prevalence of traditional cardiovascular risk factors including hypertension , diabetes/IGT or stroke than male patients.
5.Influence of Different Anticoagulant Tube on Measurement Results of Homocysteine
Jin HE ; Qin YAO ; Lujie WANG ; Jun ZHANG
Journal of Kunming Medical University 2013;(11):18-20
Objective To study the influence of different anticoagulant tube of blood for blood plasma total homocysteine (tHcy) . Methods Six healthy subjects were selected, then the fasting venous blood samples were collected with EDTA-2k tube,sodium citrate tube and lithium heparin tube, respectively. The blood samples were kept under different conditions. The concentrations of tHcy were determined by quantitative HPLC method and the data were analyzed using SPSS17.0 software. Results Under the conditions of 4℃placed for 2 h, 4 h, 7 h, and- 20 ℃ placed for 1 d, 3 d, 5 d after separating plasma, the concentrations of plasma tHcy in different anticoagulant tubes were no statistical difference. In addition, after placing the whole blood at 4℃for 2 h, 4 h, 7 h, the concentrations of plasma tHcy of separated plasma in different anticoagulant tubes were no statistical differences. Conclusion There are no significant differences in determination of the concentration of plasma tHcy when using EDTA-2k tube,sodium citrate tube and lithium heparin tube sampling blood.
6.Esterase Isozyme Analysis of 14 Strains of Ganoderma
Jun-Zhe QIN ; Shen-Rui GU ; He CHEN ;
Microbiology 1992;0(04):-
Antagonism, esterase isozyme ployacrylamide gel electrophoretic (EST-PAGE) and cluster analysis was used in the study of 14 strains of Ganoderma. The results showed that there were 18 bands of EST with different Rf. Cluster analysis at 50% similarity level showed that all strains could be clustered into five groups, the first included Xian zhi, Da xian 823, Nan han, Yuan zhi 6, Tian zhi, Han zhi; the second included Chi zhi 10, Chi zhi 12, Chi zhi, Ri ben hong zhi; the third included Zi zhi, Hei zhi; the fourth included Xue zhi; the fifth included Bai zhi. And this result was similar to the result of antagonism and morphological characters. Because of its stablity, the technique of EST can be used for identification of Gamoderma.
7.Comparison of cardiac function parameters in gated myocardial SPECT determined by filtered backprojection and OSEM reconstruction methods
Jia-jun, LI ; Yue-qin, TIAN ; Zuo-xiang, HE
Chinese Journal of Nuclear Medicine 2011;31(3):183-186
Objective To compare the cardiac function parameters in gated SPECT determined by filtered back projection (FBP) and OSEM reconstruction methods. Methods One hundred and forty-four patients underwent 99Tcm-MIBI gated-SPECT imaging studies. The parameters LVEF, EDV and ESV, were derived using quantitative gated SPECT (QGS), four-dimensional model SPECT (4D-MSPECT) and emory cardiac toolbox (ECToolbox) softwares. Each image was reconstructed by FBP or OSEM. Bland-Altman analysis and paired t-test were applied to evaluate those parameters. Results Correlation coefficients for LVEF, EDV and ESV between FBP and OSEM methods were all more than 0.93 (all P<0.001). EDV calculated by FBP was lower than that by OSEM using QGS software, but became the opposite when using 4D-MSPECT and ECToolbox softwares. (QGS: (82.2±39.1) ml vs (83.5±40.8) ml, t=-2.53, P<0.05; 4D-MSPECT: (93.5±46.9) ml vs (88.8±45.2) ml, t=5.95, P<0.01; ECToolbox: (106.4±51.1) ml vs (100.8±49.0) ml, t=3.99, P<0.01). ESV calculated by FBP was higher than that by OSEM using 4D-MSPECT software (4D-MSPECT:(37.5±41.4) ml vs (34.8±37.6) ml, t=3.92, P<0.01). LVEF calculated by FBP was lower than that by OSEM using QGS software ((62.1±16.9)% vs (63.1±16.1)%, t=-3.14, P<0.05), but higher than that by OSEM using ECToolbox software ((74.1±18.8)% vs (71.3±17.1)%, t=5.28, P<0.01). Conclusion Generally, cardiac functional parameters based on FBP and OSEM construction methods correlated well, although they might have singnificantly different results.
8.Diagnosis and Treatment of Concealed Penis in Children: a 16 Case Report
Jun QIN ; He WANG ; Jian-lin YUAN ; Baoqi CHEN ; Rongliang QIN
Chinese Journal of Rehabilitation Theory and Practice 2006;12(10):903-904
ObjectiveTo explore the diagnosis and the surgical operation of concealed penis, and raise the therapeutic efficacy.Methods16 cases of concealed penis were reviewed retrospectively.ResultsAll 16 cases were treated by Devine surgery and got the satisfactory effect, the penis recovered to its good external appearance. All 16 cases had been followed up for four months to two years. The revelation of penis and its external appearance were all satisfied. No complications such as hydrophallus, skin ischemia, haematoma and erection ache occurred.ConclusionThe real concealed penis should be strictly distinguished from buried penis, microphallus and redundant prepuce, and suitable operation pattern can obtain satisfactory effect.
9.Research progress of miR-338 in tumor
Fang HE ; Qing-Qing QIN ; Yong-Qin ZHENG ; Jun-Dong HE
Basic & Clinical Medicine 2018;38(6):840-844
MicroRNAs(miRNAs) are a class of highly conserved,endogenous,small,non-coding RNAs that reg-ulate RNAi and are key regulators of tumorigenesis and development.miR-338 is abnormally expressed in most tumors. Aberrant expression of miR-338 can affect the proliferation, invasion, metastasis, angiogenesis and other physiological activities of tumor cells and play a vital role in the regulation on one or more target-genes.
10.Building a comprehensive health assessment indicators systems for health management of military officers
Hongyan ZHANG ; Jun DONG ; Haiyun WU ; Kunlun HE ; Xiaoyong SAI ; Yao HE ; Yinhe QIN
Chinese Journal of Hospital Administration 2010;26(6):466-469
In line with the new WHO definition for health and reference of comprehensive health assessments of elderly overseas, the paper screened and set the weight for comprehensive health assessment indicators for military officers, establishing a general health assessment model as a result 402 military officers were subject to Comprehensive Health Assessments, and the outcomes were compared with subjective assessment of experts. The results of both methods are highly coincidental. It proves Comprehensive Health Assessment as a scientific quantitative health assessment method, with promising perspectives in health management in the future.