Objective:To investigate the cause and preventive measures of chemotherapy-related death from human malignant neoplasms.Methods:A retrospective study was carried out to evaluate the clinical data of 15 patients with chemotherapy-related death of human neoplasms in 2008.The cause,treatment,survival time after chemotherapy and preventive measures were analyzed.Results:The average age of the 15 patients was 52.4 years,and 5 of them were treated with docetaxel.The mean survival time after chemotherapy was 16.8 days.5 cases presented severe sehematological toxicity, and 2,breath failure.Conclusion:Chemotherapy-related death from human malignant neoplasms was correlated with disease history,age and chemotherapeutic agents,etc.;therefore,no use of docetaxel for patients aged over 60 years or with hepatic and lung diseased history could decrease the chemotherapy-related death.

Objective To determine the relationship between blood biochemical metabolites and benign prostatic hyperplasia (BPH).Methods From Jan.2008 to Oct.2010,the medical records of 73 BPH patients diagnosed by postoperative histopathology were retrospectively studied.The age of patients ranged from 55 to 86 years.The average patient age was 70 years.Of the 73 cases,65 cases were not taking medications which could have an impact on metabolic levels of serum glucose and lipoprotein.Three months prior to operation,the patients were placed in a group to analyze the correlation between the total volume of the prostate (TP),prostate average annual growth rate and biochemical metabolites including serum glucose,high-density lipoprotein cholesterol (HDL-c) and prostate specific antigen (PSA).To compare the changes of prostate volume,prostate annual growth rate and PSA,the 73 cases were divided into two groups; patients with metabolic syndrome (MS) and those without MS.Results Of the 65 BPH patients,the mean of TP and prostate annual growth rate were (58.76 ± 25.96) ml and (1.32 ± 0.89) ml,serum glucose was (4.99 ± 0.73) mmol/L,HDL-c was (1.36 ± 0.39) mmol/L,and PSA was (5.99 ±8.30) μg/L.Stratified with prostate volume,patient's serum glucose,prostate annual growth rate and PSA levels increased with the increased TP (F =4.63,P =0.0060; F =62.98,P =0.0001 ; F =4.83,P =0.0044).On the other hand.HDL-c decreased with the increased TP (F =5.32,P =0.0025).There was a positive relationship between TP and serum glucose (r =0.48,P =0.0005).There was a negative correlation between TP and HDL-c (r =-0.26,P =0.0353).Of the 73 patients,20 cases had MS.The MS group had PSA levels (6.52 ± 5.28) μg/L,TP (79.41 ± 38.24) ml and prostate annual growth rate (1.86 ± 1.14) ml.These levels were higher than the 53 cases without MS.The group without MS had PSA levels (3.95 ± 3.77) μg/L,TP (54.98 ± 20.94) ml and prostate annual growth rate (1.23 ± 0.80) ml (t=2.29,P=0.0253; t=2.65,P=0.0147; t=2.22,P=0.0360).Conclusions PSAlevels,TP and prostate annual growth rate in BPH patients are related with blood biochemical metabolites.Both serum glucose and HDL-c affect prostate volume.BPH patients with MS have a remarkable clinical progression.

Objective To explore the clinical effect of the percutaneous vertebroplasty(PVP) and filling auto-solidification calcium phosphate cement(CPC) in treatment of osteoporotic thoracolumbar vertebral fractures. Methods From January, 2004, 26 patients with osteoporotic thoracolumbar vertebral compression fracture (29 vertebral bodies) were treated with intraoperative manipulative reduction, PVP and filling auto-solidification CPC. Results PVP were successfully performed with unilateral or bilateral vertebral pedicles for the 29 vertebrae. The average injection dose of CPC was 4.6 mL. CPC leakage occurred during the procedure in 5 vertebrae of 5 cases. All the patients were followed up for 3 to 8 months (averaging 4.6 months) and experienced complete pain relief. There was no severe complication related to the treatment. Except in 3 patients who lost 15%of the vertebral body height, the anterior height of the injured vertebral body in the other 23 patients recovered averagely to 80%of the normal one. Conclusion PVP with filling auto-solidification CPC is safe and effective in the treatment of osteoporotic thoracolumbar vertebral compression fractures.

Purpose:To explore the therapeutic effects of combining chemotherapy and TCM recipe “Xiao Zheng Fang” for advanced colorectal cancer.Methods:A prospective and comparable clinical study was made in 85 cases of advanced colorectal carcinoma seen over 6 years , Who were not suitable for radical operation or had metastases after operation. Survival time,life quality, objective effect and blood flow rate indexes were studied and analysed. Results:Integrated therapy of tranditional Chinese and Western medicine is superior to chemotherapy alone in life quality ( P

Objective To evaluate the efficacy and clinical feasibility of the computer-assisted full plan- ning system for tibial fracture treatment with intramedullary nailing.Methods After analyzing the functional structure and operative procedures of the system,nine plastic tibia and 12 cadaver lower limbs were used for image mosaicing based on C-arm (PHILIPS BV Libra) fluoroscopic images in the operation room to assess the correctness of the mosaicing and planning algorithms.The plastic tibial model was used for analysis of the mosaicing precision.The cadaver tibial bone was used for reduction experiment with the reduction mechanism to analyze the operation feasi- bility.Results Only 7 to 10 [fluoroscopy time:(19.75?0.61)s] valid C-ann projection images were needed to produce a long bone panorama of the lower limb.The total time for image acquisition and mosaicing was within (4.17?0.86)minutes and the mosaicing precision in the plastic tibial model was (1.26?0.76)mm.The opera- tion of the reduction mechanism was very simple and could be controlled by a surgeon automatically or free of hand. An integrated reduction strategy could be produced for rough positioning in general and elaborate operations in de- tails.Conclusion The computer-assisted full planning system can be used for anatomical analysis based on the C-arm panorama,full surgical planning,virtual simulation,selection of proper intramedullary nails and fracture reduction in treatment of long bone fractures.

Objective To compare the differences in mucin-histochemistry and etiology of Barrett's esophagus (BE) intestinal metaplasia (IM) , cardiac intestinal metaplasia (CIM) and gastric antrum intestinal metaplasia (GA-IM). Methods Alcian blue /periodic acid-schiff (AB/PAS) and high iron diamine / alcian blue (HID/AB) histochemical methods were used to classify IM in BE, cardia and gastric antrum, and IM were classified into three subtypes: complete small intestinal type (type Ⅰ ) , incomplete small intestinal type (type Ⅱ) and incomplete colonic type (type Ⅲ). Compared the prevalence of different subtypes of IM in above-mentioned sites, and investigated their relationships among the symptoms of gastroesophageal reflux disease (GERD) and Helicobacter pylori (Hp) infection. Results The prevalence of type Ⅲ IM in long-segment BE (LSBE) and short-segment BE (SSBE) is 75. 0% and 63. 3% respectively, it is significantly higher than that in CIM (23. 1% ) and GA-IM ( 17. 7% ) (P

Objective To investigate the preventive effects of anti-viral therapy in combination with transcatheter arteria chemoembolization (TACE) on recurrence of HBV-related hepatocellular carcinoma (HCC) after operation. Methods According to selection standards, 49 patients treated in this hospital from January 2003 to June 2009 were enrolled in this study and divided into a control group and combined group.The cumulative tumor-free survival rate, cumulative survival rate, the 1-, 2- and 3-year recurrence rates and cumulative recurrence rates were determined in all the patients and compared between the 2 groups. Results The cumulative tumor-free survival rate and cumulative survival rate were significantly higher in the combined group than in the control group (P=0. 019, 0. 008). The 1-, 2-, and 3-recurrence rates were 24% vs. 38.9%, 28% vs. 33.3%, and 28% vs. 22.2% in the combined group and control group, respectively. The 1-, 2-, and 3-year cumulative recurrence rates were 24% vs. 38. 9%, 52% vs. 72. 2%, and 80% vs.94. 4% in the combined group and control group, respectively. Conclusion Anti-viral therapy in combination with TACE can exert significant preventive effects on recurrence of HBV-related HCC after operation.

Objective To study the effect of antisense RNA retroviral vector of mTOR on the proliferation of vascular smooth muscle cells(VSMC).Methods The conservative region of mTOR gene was inserted into pLXIN reversedly,then the vector was packaged in PT67 cells by transfection with lipofectamine and(transfected) to VSMC.The efficiency of antisense inhibition was verified,and the changes of p70S6k and(4E-BP1) were also determined at the same time.The proliferation activity was determined by flowcytometry and MTT.Results After the vector was successfully transfected to the cells.pLXIN-A-mTOR vector could(efficiently) decrease mRNA and protein expression of mTOR and p70S6k of the cell,while the expression of 4E-BP1 increased significantly.The cell cycle and proliferation activity of VSMC was also stunned in phase G_1.Conclusions The mTOR antisense retroviral vector,pLXIN-A-mTOR,has been constructed(successfully),which can significantly inhibit the proliferation of VSMC.

Objective To study the expression of mammalian target of rapamycin (mTOR) in autogenous vein graft. Methods Autogenous vein graft model was established in 64 Wistar rats. Graft vein was harvested 6 hours, 1 day, 3 days, 1 week, 2 weeks, 4 weeks, 6 weeks and 8 weeks after transplantation. mTOR mRNA was measured by RT-PCR and in situ hybridization. Western blotting and immunohistochemistry methods were used to detect the expression of mTOR. PCNA expression was detected. Results The vein graft mRNA and protein expression of mTOR increased soon after transplantation. mTOR mRNA reached the peak 1 to 2 weeks after surgery [(48?18)% and (33?11)% respectively, P

Objective To investigate the expression of c-Jun N-terminal kinase (JNK) and p38MAPK in autogenous vein graft during vascular remodeling. Methods Autogenous vein graft model was established in 80 Wistar rats. Vein graft samples were harvested at 6th hour, day 1, day 3, day 7, day 14, day 28, day 42 and day 56. Gene expression of JNK and p38MAPK were measured by reverse transcription-PCR and in situ hybridization. Western blot and immunohistochemistry were used to detect the protein expression and phosphorylation of JNK and p38 MAPK. Apoptosis of VSMCs was studied by TUNEL. Results The expression of JNK mRNA and p38 mRNA reached the highest level on the second week (26?10)% and (59?26)% respectively, P