The exon 1 of the human androgen receptor gene contains CAG(polyglutamine) repeat length polymorphism. The CAG repeat sequence is closely relevant to many diseases. In this article, we reviewed the relationship between androgen receptor gene(CAG)n polymorphism anti primary liver cancer.

10.3969/j.issn.2095-4344.2013.25.019

AIM： To investigate the effects of 6， 7－ dimethoxycoumarins on the proliferation and the cell cycle of human lung carcinoma cell（ PA） in vitro METHODS： The effects of 6， 7－ dimethoxycoumarins on the cytomorphology， growth and cell cycle of PA cells were evaluated by MTT assay and flow cytometry in vitro RESULTS： 6， 7－ dimethoxycoumarins could inhibit the proliferation of PA cells in a dose－ dependent manner The inhibition action of 6， 7－ dimethoxycoumarins plateaued at 160μ g/ml(52 4％ inhibition) The agent in dose of 80μ g/ml led to a decline in the proportion of cells to those in G2/M phase， made the cells remain in G0/G1 phase and reduced the proliferation index(PI) of PA cells CONCLUSION： In vitro， 6， 7－ dimethoxycoumarins can inhibit the proliferation of PA cells， which leads to arrest of the cells in G0/G1 phase by way of inhibiting DNA synthesis

AIM:To investigate the effects of 6,7-dimethoxycoumarins on the proliferation and the cell cycle of human lung carcinoma cell(PA)in vitro METHODS:The effects of 6,7-dimethoxycoumarins on the cytomorphology,growth and cell cycle of PA cells were evaluated by MTT assay and flow cytometry in vitro RESULTS:6,7-dimethoxycoumarins could inhibit the proliferation of PA cells in a dose-dependent manner The inhibition action of 6,7-dimethoxycoumarins plateaued at 160?g/ml(52 4% inhibition) The agent in dose of 80?g/ml led to a decline in the proportion of cells to those in G2/M phase,made the cells remain in G0/G1 phase and reduced the proliferation index(PI) of PA cells CONCLUSION:In vitro,6,7-dimethoxycoumarins can inhibit the proliferation of PA cells,which leads to arrest of the cells in G0/G1 phase by way of inhibiting DNA synthesis

From the view of medical professionals, this paper explored the standards of residents′ competency cultivation in Zhuhai using Delphi method. The research outcome that consisted of 53 factors and 6 dimensions was obtained after two rounds of expert consultation. There are 6 first-level indexes including professionnalism and body-mind quality, clinical practice skill, the competency of lifelong learning and self-improvement, humanistic medicine practice skill, medical knowledge, and the competency of promoting the medical system to develop. As well, there are 18 second-level indexes and 29 third-level indexes. The standards stated that the clinical prac-tice skill would not be the main part of residents′competency cultivation. Qualified residents should reach the na-tional standard of the clinical practice skill but also the other five indexes.

Objective To investigate the diagnosis and treatment of Mirizzi syndrome.Methods 6 cases of Mirizzi syndrome were analyzed with review of the latest literatures on its diagnosis and treatment.Results None of the 6 cases were definitely diagnosed by ultrasonography and CT.All of them underwent operations,five of them were cured and one died.Conclusions Ultrasonography and CT can give a hand to diagnosis.Definite diagnosis depends on ERCP and PTC.The specific conditions of the patients and the experience of surgeon decide the selection of treatment.Subtotal cholecystectomy and hepaticojejunostomy are the basic choices.

To analyze the clinical characteristics,complications and management of Brucellosis.As a retrospective review,23 patients with Brucellosis admitted into Peking University First Hospital over a 10-year period were reviewed.Most of them were middle-aged males.Common presenting clinical manifestations included high fever,sweating,arthralgia and headache.Splenomegaly was more common than hepatomegaly.There were liver injury (n =15),transient proteinuria (n =6),secondary hemophagocytic syndrome (n =2) and renal failure with infective endocarditis,pneumonia with infective endocarditis,renal abscess (n =1).Most of them were treated effectively with combination therapy.The clinical features of Brucellosis are both complicated and atypical.

Objective:To study the influence of miR-221 in theβcells of mice with diabetic nephropathy (DN), and to clarify the protective effect of miR-221 on DN.Methods:Twenty wild type C57BL/6J mice aged 8 weeks were divided into control group and DN group (n=10).The DN mice models were constructed with 14 weeks of high fat diet,and 100 mg·kg-1 streptozotocin (STZ)was used to induce the partial insulin deficiency.10 weeks later the blood glucose,serum creatinine,blood urea nitrogen content and 24h-urinary albumin excretion rate were detected.Theβcells of islet were isolated from the DN mice.The expression level of SOCS3 mRNA inβ cells of islet was valued by Real-time PCR.The proliferation of pancreaticβcells of islet was examined by MTT assay.The contents of insulin and insulin release levels in pancreaticβ cells of islet were detected by ELISA assay.Luciferase reporter gene assay was used to detect the luciferase reporter gene activity of SOCS3.Results:The DN mouse models were constructed successfully.The blood glucose,serum creatinine,blood urea nitrogen and 24h-urinary albumin excretion rate of the mice in DNA group were higher than those in control group (P < 0.05 ).After overexpression of miR-221,the expression level of SOCS3 mRNA in pancreaticβcells of islet and the proliferation ability ofβcells of islet of the mice in DN group were lower than those of normal islet cells (P < 0.05).Then luciferase reporter gene method found SOCS3 as one of the target genes of miR-221.After overexpression of miR-221,the insulin release level and insulin content in pancreaticβcells of islet were higher than those of normal islet cells (P <0.05).Conclusion:miR-221 can promopt the synthesis and secretion ofβ cells of islet and inhibit the dysfunction ofβcells of islet in the DN mice by down-regulating the SOCS3 level.

Objectiye To improve the diagnosis and treatment of chromophobe renal cell carcinoma(CRCC). Methods The clinical dota of 25 patients of CRCC were reviewed.Thirteen were xmales and 12 were females and thirteen on the left and twelve on the right.The mean age was 51 years.Sixteen(64%)patients were asymptomatic.Gross hematuria,low back pain and discomfort and fever occurred in the other 9 patients.Laboratory investigations showed 1 patient had raised alanine aminotransferase and 1 had high erythrocyte sedimentation rate. Results B-ultrasound was mainly characterized by low echo renal mass with intact capsule and low blood flow signals.CT and MR of CRCC were typically well circumscribed,homogeneous(unenhanced CT was 70%,MR was 73%)with no necrosis and hemorrhage,homogenous enhancement(CT was 65%,MR was 67%)and mild enhanced(CT was 65%,MR was 67 %)renal mass.Twenty-two patients with tumors＞4.0 cm had radical nephrectomy and three with tumors≤4.0 cm had partial nephrectomy.The average diameter of tumors was 7.6 cm.The cross-sections of the tumors were grossly homogeneous,pale or dark brown solid.Light microscopy showed that the tumors were composed of trabeculae or sheets with voluminous cells in pale or eosinophilic cytoplasm.Immunohistochemical assay was positive of CK8 and negative of Vimentin.The pathologic TNM stages were 8 with T1a,9 with T1b,6 with T2 and 2 with T3a.Twenty-three patients were followed up.After mean follow-up of 28 months,22 cases were tumor free.One patient had pulmonary metastasis 58 months after operation and had no reaction to interferon-α and chemotherapy and died. Conclusions The majority of CRCC patients are asymptomatic and usually with low-stages.There are some features in CT and MR appearance of CRCC such as well circumscribed and homogenous.Surgical treatment should follow the treatment principles of renal cell carcinoma and carries an excellent prognosis for most localized tumors but there has been no effective measures to treat metastasis disease.The interval between operation and metastasis is relatively long and the time of follow-up should be prolonged in CRCC.

Objective To evaluate the role of real-time three-dimensional transesophageal echocardiography(RT3D-TEE) in non-valvular atrial fibrillation with left atrial appendage(LAA) stunning after catheter ablation.Methods Clinical and echocardiographic variables were analyzed by univariate regression in order to investigate the relationship between the group of LAA stunning and others in 28 patients after catheter ablation.Results Univariate analysis revealed difference in persisting time of atrial fibrillation[(16.4 ± 11.6)weeks vs (21.3 ± 18.6) weeks,P ＜0.05],left atrial diameter[(43.4 ± 8.3) mm vs (47.6 ± 5.9) mm,P ＜0.05 ],left atrial emptying fraction (0.38 ± 0.04 vs 0.30 ± 0.09,P ＜0.05).LAA emptying fraction based on three-dimensional volume measurements had significant difference (0.20 ± 0.03 vs 0.12 ± 0.02,P ＜ 0.001) between the group of LAA stunning and other cases.Conclusions LAA ejection fraction calculation by RT3D-TEE is feasible and more accurate than by clinical and other echocardiographic in LAA stunning after ablaton of atrial fibrillation.