1.Relationship between androgen receptor gene (CAG)n polymorphism and primary liver cancer
Jun LI ; Zhijun DAI ; Zongfang LI
International Journal of Surgery 2009;36(2):97-99
The exon 1 of the human androgen receptor gene contains CAG(polyglutamine) repeat length polymorphism. The CAG repeat sequence is closely relevant to many diseases. In this article, we reviewed the relationship between androgen receptor gene(CAG)n polymorphism anti primary liver cancer.
2.A membrane controlled release drug delivery system promotes injured tissue repair
Wei LI ; Jianghua DAI ; Jun LUO ; Min DAI ; Qiankun GAO
Chinese Journal of Tissue Engineering Research 2013;(25):4699-4706
10.3969/j.issn.2095-4344.2013.25.019
3.Effects of 6, 7- dimethoxycoumarins on Cell Proliferation and Cell Generation Cycle of Human Lung Car_cinoma Cell in Vitro
Yunzhong TAN ; Guohua DAI ; Jun LI
China Pharmacy 2001;12(5):267-268
AIM: To investigate the effects of 6, 7- dimethoxycoumarins on the proliferation and the cell cycle of human lung carcinoma cell( PA) in vitro METHODS: The effects of 6, 7- dimethoxycoumarins on the cytomorphology, growth and cell cycle of PA cells were evaluated by MTT assay and flow cytometry in vitro RESULTS: 6, 7- dimethoxycoumarins could inhibit the proliferation of PA cells in a dose- dependent manner The inhibition action of 6, 7- dimethoxycoumarins plateaued at 160μ g/ml(52 4% inhibition) The agent in dose of 80μ g/ml led to a decline in the proportion of cells to those in G2/M phase, made the cells remain in G0/G1 phase and reduced the proliferation index(PI) of PA cells CONCLUSION: In vitro, 6, 7- dimethoxycoumarins can inhibit the proliferation of PA cells, which leads to arrest of the cells in G0/G1 phase by way of inhibiting DNA synthesis
4.Retrospective analysis of clinical profiles of Brucellosis
Lihua DAI ; Jun LI ; Xiaoyuan XU
Chinese Journal of General Practitioners 2013;(7):562-563
To analyze the clinical characteristics,complications and management of Brucellosis.As a retrospective review,23 patients with Brucellosis admitted into Peking University First Hospital over a 10-year period were reviewed.Most of them were middle-aged males.Common presenting clinical manifestations included high fever,sweating,arthralgia and headache.Splenomegaly was more common than hepatomegaly.There were liver injury (n =15),transient proteinuria (n =6),secondary hemophagocytic syndrome (n =2) and renal failure with infective endocarditis,pneumonia with infective endocarditis,renal abscess (n =1).Most of them were treated effectively with combination therapy.The clinical features of Brucellosis are both complicated and atypical.
5.Diagnosis and treatment of Mirizzi syndrome
Jun LI ; Zhongqin DAI ; Guangmin WANG
Journal of Clinical Surgery 2001;0(01):-
Objective To investigate the diagnosis and treatment of Mirizzi syndrome.Methods 6 cases of Mirizzi syndrome were analyzed with review of the latest literatures on its diagnosis and treatment.Results None of the 6 cases were definitely diagnosed by ultrasonography and CT.All of them underwent operations,five of them were cured and one died.Conclusions Ultrasonography and CT can give a hand to diagnosis.Definite diagnosis depends on ERCP and PTC.The specific conditions of the patients and the experience of surgeon decide the selection of treatment.Subtotal cholecystectomy and hepaticojejunostomy are the basic choices.
6.Study on the Standards of Residents′Competency Cultivation in Zhuhai
Chinese Medical Ethics 2016;29(5):755-758
From the view of medical professionals, this paper explored the standards of residents′ competency cultivation in Zhuhai using Delphi method. The research outcome that consisted of 53 factors and 6 dimensions was obtained after two rounds of expert consultation. There are 6 first-level indexes including professionnalism and body-mind quality, clinical practice skill, the competency of lifelong learning and self-improvement, humanistic medicine practice skill, medical knowledge, and the competency of promoting the medical system to develop. As well, there are 18 second-level indexes and 29 third-level indexes. The standards stated that the clinical prac-tice skill would not be the main part of residents′competency cultivation. Qualified residents should reach the na-tional standard of the clinical practice skill but also the other five indexes.
7.Effects of 6,7-dimethoxycoumarins on Cell Proliferation and Cell Generation Cycle of Human Lung Carcinoma Cell in Vitro
Yunzhong TAN ; Guohua DAI ; Jun LI
China Pharmacy 1991;0(05):-
AIM:To investigate the effects of 6,7-dimethoxycoumarins on the proliferation and the cell cycle of human lung carcinoma cell(PA)in vitro METHODS:The effects of 6,7-dimethoxycoumarins on the cytomorphology,growth and cell cycle of PA cells were evaluated by MTT assay and flow cytometry in vitro RESULTS:6,7-dimethoxycoumarins could inhibit the proliferation of PA cells in a dose-dependent manner The inhibition action of 6,7-dimethoxycoumarins plateaued at 160?g/ml(52 4% inhibition) The agent in dose of 80?g/ml led to a decline in the proportion of cells to those in G2/M phase,made the cells remain in G0/G1 phase and reduced the proliferation index(PI) of PA cells CONCLUSION:In vitro,6,7-dimethoxycoumarins can inhibit the proliferation of PA cells,which leads to arrest of the cells in G0/G1 phase by way of inhibiting DNA synthesis
8.Protective effect of miR-221 onβcells in mice with diabetic nephropathy and its mechanism
Jun LI ; Liuling DAI ; Ye LI ; Chunli CHA
Journal of Jilin University(Medicine Edition) 2016;42(5):905-909
Objective:To study the influence of miR-221 in theβcells of mice with diabetic nephropathy (DN), and to clarify the protective effect of miR-221 on DN.Methods:Twenty wild type C57BL/6J mice aged 8 weeks were divided into control group and DN group (n=10).The DN mice models were constructed with 14 weeks of high fat diet,and 100 mg·kg-1 streptozotocin (STZ)was used to induce the partial insulin deficiency.10 weeks later the blood glucose,serum creatinine,blood urea nitrogen content and 24h-urinary albumin excretion rate were detected.Theβcells of islet were isolated from the DN mice.The expression level of SOCS3 mRNA inβ cells of islet was valued by Real-time PCR.The proliferation of pancreaticβcells of islet was examined by MTT assay.The contents of insulin and insulin release levels in pancreaticβ cells of islet were detected by ELISA assay.Luciferase reporter gene assay was used to detect the luciferase reporter gene activity of SOCS3.Results:The DN mouse models were constructed successfully.The blood glucose,serum creatinine,blood urea nitrogen and 24h-urinary albumin excretion rate of the mice in DNA group were higher than those in control group (P < 0.05 ).After overexpression of miR-221,the expression level of SOCS3 mRNA in pancreaticβcells of islet and the proliferation ability ofβcells of islet of the mice in DN group were lower than those of normal islet cells (P < 0.05).Then luciferase reporter gene method found SOCS3 as one of the target genes of miR-221.After overexpression of miR-221,the insulin release level and insulin content in pancreaticβcells of islet were higher than those of normal islet cells (P <0.05).Conclusion:miR-221 can promopt the synthesis and secretion ofβ cells of islet and inhibit the dysfunction ofβcells of islet in the DN mice by down-regulating the SOCS3 level.
9.Construction and evaluation of intracerebral capsular hemorrhage models in rats
Rufei DAI ; Jun CAI ; Ning LIU ; Fengyi ZHU ; Xiaoming LI
Chinese Journal of Tissue Engineering Research 2006;10(42):211-213
BACKGROUND: A stable and exact animal model is the necessary tool and basis for studying hemorrhagic cerebrovascular diseases.OBJECTIVE: To establish and evaluate the intracerebral capsular hemorrhage models in rats.DESIGN: A randomized and controlled animal experiment.SETTING: Second Hospital Affiliated to Xuzhou Medical College; First Hospital Affiliated to Nanjing Medical University.MATERIALS: This experiment was carried out in the animal experimental center of Nanjing Medical University during May to November 2002.Totally 35 SD rats were randomized into two groups: experimental group (n=30) and sham-operation group (n=5).METHODS: ① Autoblood was injected into the intracerebral capsule of rats to create intracerebral capsule hemorrhage models with stereotaxy in the experiment group. ②Scoring was conducted according to 5-point neurological scoring criteria from ZeaLonga, somatic sensation and motor function of rats were observed. ③Somatosensory evoked potential(SEP) of rats was detected pre- and post-operation under anesthetic state. ④ After determination of SEP, the rats were sacrificed under anesthetic state. Brains were taken out to made slices, then sections were stained with haematoxylin and eosin. Changes in haematoma and histomorphology were observed at the largest focus under optical microscope.MAIN OUTCOME MEASURES: ①Nerve function scoring; ②Latent period of various waves of SEP; ③ Observation of brain tissue morphology.RESULTS: Totally 35 rats entered the stage of result analysis. ①Appearance of obvious paralysis of the rats suggested the modeling was successful. The successful rate of this experiment was 93.3%(28/30). Significant difference existed in neurological scoring between experimental group [(2.74±0.46)points] and sham-operation group (0 point)(P<0.05). ②SEP showed that the latent periods of various waves of experimental group after operation were significantly delayed than those before operation and those of sham-operation group [P1: (15.72±0.78) ms, (10.69±0.52) ms, (10.73±0.48) ms;Nl: (17.95±1.27) ms,(13.21±1.31) ms, (13.34±1.27) ms;N2:(21.16±1.62) ms, (15.42±1.46) ms,(15.58±1.44) ms;N3:(24.86±1.58) ms, (18.72±1.76) ms, (18.99±1.67) ms,P<0.05]. ③In the shamoperation group, a few red blood cells were scattered in the peripheral area of needle channel were found, but hemorrhagic focus was not; In the experimental group, irregular or oval blood clots presented in the left internal capsule area. In about a low-fold visual field, brain tissue in the surrounding of hemorrhagic focus was loosened and swelled, and pathological changes were obviously severer than those in the sham-operation group.CONCLUSION: Intracerebral capsular hemorrhage induced by injection of autoblood with stereotaxy is more close to clinical situation, and it is easy to operate and has good reproducibility.
10.Assessment of left atrial appendage stunning by real-time three-dimensional transesophageal echocardiography after ablation of atrial fibrillation
Dajun QIAN ; Jun TAN ; Min DAI ; Kunlin LI ; Ruxing WANG
Chinese Journal of Ultrasonography 2012;21(4):288-291
Objective To evaluate the role of real-time three-dimensional transesophageal echocardiography(RT3D-TEE) in non-valvular atrial fibrillation with left atrial appendage(LAA) stunning after catheter ablation.Methods Clinical and echocardiographic variables were analyzed by univariate regression in order to investigate the relationship between the group of LAA stunning and others in 28 patients after catheter ablation.Results Univariate analysis revealed difference in persisting time of atrial fibrillation[(16.4 ± 11.6)weeks vs (21.3 ± 18.6) weeks,P <0.05],left atrial diameter[(43.4 ± 8.3) mm vs (47.6 ± 5.9) mm,P <0.05 ],left atrial emptying fraction (0.38 ± 0.04 vs 0.30 ± 0.09,P <0.05).LAA emptying fraction based on three-dimensional volume measurements had significant difference (0.20 ± 0.03 vs 0.12 ± 0.02,P < 0.001) between the group of LAA stunning and other cases.Conclusions LAA ejection fraction calculation by RT3D-TEE is feasible and more accurate than by clinical and other echocardiographic in LAA stunning after ablaton of atrial fibrillation.