1.A Study of Epidermal Apoptosis.
Young Lip PARK ; Kyu Uang WHANG ; Jun Hong PARK
Korean Journal of Dermatology 1998;36(1):59-70
BACKGROUND: Apoptosis is a highly selective form of cell suicide with characteristic morphologieal and biochemical features, including chromatin condensation, formation of apoptotic bodies, and DNA fragmentation by the activation of endonucleases. Various cytokines and physical or chemical factors can provoke apoptotic changes in the skin. OBJECTIVE: We investigated the cytotoxic effects with epidermal cytokines and their combinations, K+ ionophores, protein synthesis inhibitor(emetine), inhibitor of endogenous endonuclease(aurintricarboxylic acid, ATA), sodium azide, and retinoic acid witp human epithelial tumor cell lines(A431 cells) to examine the degree of induction of apoptosis in the epidermal keratinocytes. METHODS: Induction of apoptosis was measured in cultured human keratinocytes, keratinocyte cell lines(A-431, HaCat, KB cells), cultured human melanocytes and malignant melanoma cell lines(SK-28, SK-30) using a mixture of ethidium bromide and acridine orange, DNA agarose gel electrophoresis and TUNEL staining. RESULTS: l. In the A-431 cells, (1 to a certain degree, the combination of IFN-gamma and TNF-alpha could only induce apoptosis. Q2 most of K+ ionophores were observed to induce necrosis rather than apoptosis. Q3 emetine, a protein synthesis blocker, was found to induce apoptosis in a dose-dependent pattern. Q4 sodium azide at a concentration of 1% .induced apoptosis rather than necrosis. Q5 retinoic acid inhibit the beuvericin induced apoptosis. 2. In human keratinocytes, Ql more resistant in the induction of apoptosis than any cultured keratinocyte cell lines p aurintricarboxylic acid(ATA)-an endonuclease inhibitor, could inhibit UV induced apoptosis 3. In human keratinocytes and cultured keratinocyte cell lines, c-PAF inhibit the beauvericin induced apoptosis. 4. Human melanocytes is very resistant for the induction of apoptosis by beauvericin. 5. In the melanocytes and melanoma cell lines, sodium azide and beauvericin induced necrosis rather than apoptosis. CONCLUSIONS: The epidermis is continuously exposed to toxic factors which might induce cell death. With the above results, the induction of appeared to be rather resistant, epidermal cell apoptosis which may reflect the existence of some endogenous protective mechanisms in the epidermis to survive at certain toxic environments; melanocytes showed high expression of bcl-2 protein which could play a role in endogenous defense against toxic environments of the epidermis.
Acridine Orange
;
Apoptosis*
;
Cell Death
;
Cell Line
;
Chromatin
;
Cytokines
;
DNA
;
DNA Fragmentation
;
Electrophoresis, Agar Gel
;
Emetine
;
Endonucleases
;
Epidermis
;
Ethidium
;
Humans
;
In Situ Nick-End Labeling
;
Ionophores
;
Keratinocytes
;
Melanocytes
;
Melanoma
;
Necrosis
;
Skin
;
Sodium Azide
;
Suicide
;
Tretinoin
;
Tumor Necrosis Factor-alpha
2.Treatment of flail chest with Judet's strut: 56 case report.
Byung Soon PARK ; Hong Kyu KIM ; Dong Jun LEE
The Korean Journal of Thoracic and Cardiovascular Surgery 1992;25(12):1523-1529
No abstract available.
Flail Chest*
3.A Case of Squamous Cell Carcinoma and Sebaceous Carcinoma Arising from Bowen' s Disease.
Jong Jun PARK ; Hun CHUNG ; In Kyung KANG ; Kyu Chul CHOI
Korean Journal of Dermatology 1995;33(3):575-579
Bowens disease is generally regarded as a premalignant dermatosis. If untreated, 3% to 5% of patients may develop squamous cell carcinoma. However, sebaciou carcinoma arising from Bowens disease is very rare. We presented a case of quarnous cell carcinoma and sebaceous circ s disease in a 68 year-old male. He had multiple bowenoid skin lesi nsties. A bean-sized nodule as developed on the bowenoid lesion of the he had a large yellow crust.ed exudative tumor on the Rt. thigh. We took a biopsy specimen of these three discrete lesions. The nu lipid stain of frozen section revealed Bowens disease, squarnous concllnoma arising from Bowen on the trunk and extremi Rt. lower abdomen. Almost of routine histology and carcinoma, and sebaceous carcinoma, respectively.
Abdomen
;
Aged
;
Biopsy
;
Bowen's Disease
;
Carcinoma, Squamous Cell*
;
Frozen Sections
;
Humans
;
Male
;
Skin
;
Skin Diseases
;
Thigh
4.Strangulated obturator hernia.
Young Seok PARK ; Yong Il KIM ; Kyu Young JUN
Journal of the Korean Surgical Society 1991;40(1):132-135
No abstract available.
Hernia, Obturator*
5.Biological factors influencing the fate of onlay bone graft on the craniofacial skeleton.
Jun Hyung KIM ; Jin Sung KANG ; Kwan Kyu PARK
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1998;25(4):557-565
The superior volume maintenance of membranous over endochondral bone grafts, which was shown in several studies has provided the basis for its preferred clinical use as an onlay grafting material on the craniofacial skeleton. The scientific rationale for this seeming embryological advantage, however, has never been proven, Since the cortical component of membranous bone is proportionally greater than that of endochondral bone, it follows that membranous grafts would show greater volume maintenance over time. Our hypothesis is that the pattern of onlay bone graft resorption is primarily determined by a graft's micro-architecture (relative cortical and cancellous composition) rather than its embryololgical origin(membranous versus endochondral). Fourty adult New Zealand white rabbits were used for this study. There were 8 animals in each of 4 groups. The rabbits of each group were sacrificed at 3, 8, and 16 weeks. Four types of grafts were placed subperosteally, onto each rabbit's cranium: a hydroxyapatite, a cortical bone graft of membranous origin, a cortical bone graft of endochondral origin and a cancellous bone graft of endochondral origin. Membranous bone grafts were obtained from the lateral mandible and endochondral bone grafts were obtained from the ileum. In order to determine post-sacrifice volume and density of the bone grafts, a caliper technique and bone densitometry(bone densitometer: LUNAR, DPX-L, U.S.A.) were performed on all of the bone grafts. Bone graft specimens were histologically examined at 3, 8, and 16 weeks.The measurement of volume and density show that there is a statistically greater resumption in the cancellous endochondral bone grafts for all parameter, compared to either the endochondral or membranous cortical bone grafts or hydroxyapatite at all time points(p< 0.05). In addition, there is no significant difference in the resorption rates between the endochondral and membranous cortical bone grafts for all parameters at all time points. By placing cortical bone grafts and cancellous bone grafts on the recipient sites separately, we have shown that the former grafts maintain their volumes, widths and projections significantly better than the latter grafts. Futhermore, we found no statistical difference in resorption rates between the two cortical bone grafts of different embryologic origins, a finding which has never been previously shown. Bone volume fraction, measured with bone densitometry, was shown to be higher in cortical bone than in cancellous bone at all time points, further illustrating the differences between cortical and cancellous bone.From our results, we believe cortical bone to be a superior onlay-graftiong material, independent of its embryololgic origin.
Adult
;
Animals
;
Biological Factors*
;
Densitometry
;
Durapatite
;
Humans
;
Ileum
;
Inlays*
;
Mandible
;
Rabbits
;
Skeleton*
;
Skull
;
Transplants*
6.Comparative analysis of operative procedures in rectal prolapse between perineal and abdominal approach.
Ho Jin PARK ; Byung Jo SO ; Kyu Young JUN
Journal of the Korean Surgical Society 1991;40(3):354-359
No abstract available.
Rectal Prolapse*
;
Surgical Procedures, Operative*
7.Primary Cutaneous Cryptococcosis Successfully Treated With Fluconazole.
Jun Hyoung PARK ; Young Wook RYOO ; Kyu Suk LEE
Annals of Dermatology 2000;12(2):148-151
We report a case of primary cutaneous cryptococcosis on Rt. forehead and perioral area of 57 year old woman with non-insulin dependent diabetes mellitus and Lt. cerebral infarction. She had large ulcers with yellowish purulent exudates on Rt. forehead and perioral area for 2months. A histopathological examination from the lesion showed numerous encapsulated, round spores and the organisms were identified as Cryptococcus neoformans in a series of fun-gal studies. The patient received a 5-week course of IV and oral fluconazole with resolution of her skin lesion. The patient is free of any lesion several months after completing therapy. This experience supports the use of fluconazole as initial and single therapy in primary cutaneous cryptococcosis.
Cerebral Infarction
;
Cryptococcosis*
;
Cryptococcus neoformans
;
Diabetes Mellitus
;
Exudates and Transudates
;
Female
;
Fluconazole*
;
Forehead
;
Humans
;
Skin
;
Spores
;
Ulcer
8.A Case of Sturge-Weber Syndrome.
Jun Taek PARK ; Geom Hyun JANG ; Jae Kyu LEE ; Kyu Eun LEE ; Jung Hyup OH
Journal of the Korean Pediatric Society 1983;26(8):823-828
No abstract available.
Sturge-Weber Syndrome*
9.Complete remission of maxillary and infratemporal squamous cellcarcinoma after induction chemotherapy.
Jong Ryoul KIM ; One Ryong MOON ; Sang Jun PARK ; Uk Kyu KIM ; Dong Kyu YANG
Journal of the Korean Association of Oral and Maxillofacial Surgeons 1992;18(1):91-97
No abstract available.
Induction Chemotherapy*
10.A case of chronic mucocutaneous candidiasis associated with hypoparathyroidism.
Dong Won KIM ; Jun Kyu OH ; Kyu Han KIM ; Kyoung Chan PARK ; Hoan Jong LEE
Korean Journal of Dermatology 1993;31(5):755-759
Chronic mucocutaneous randidiasis is a clinical syndrome characte ized by chronic and reccurent superficial candidal infection of the skin, mucous membranes, and nails. This syndrome is frequently associated with immune deficiency or endocrinopathy, especially hypopar; thyroidism. We report a case of chrcinic mucocutaneous candidiasis associated with hypoparathyroidism in a 8- year-old girl.
Candidiasis
;
Candidiasis, Chronic Mucocutaneous*
;
Female
;
Humans
;
Hypoparathyroidism*
;
Mucous Membrane
;
Skin
;
Thyroid Gland