Process management is discussed on purchase and supply of high-cost medical consumables in hospitals.Reserve material-circulation is applied to purchase & supply management,i.e.consumables is clinically used before in-storeroom and out-storeroom examination on the basis of normative purchase.Problems brought by various standards and types of consumables are solved including difficulties in stocking goods,huge amount of money tied up,unreasonable consumption,etc.Process management facilitates clinical application,standardizes management and enhances benefits.

The composition of the occupation pressure confronted by medical material purchasing agent are analyzed from the aspects of purchasing duty,knowledge upgrade,purchasing coordination,purchasing environment,etc.To alleviate the negative effects from occupation pressure,the countermeasures and the idea of harmonious purchasing are raised.

Objective:To investigate the effects of anti-CXCR4 monoclonal antibody 12G5 on adhesion and invasion of SGC7901 cells.Methods:The effects of 12G5(5?g/ml,10?g/ml and 20?g/ml, respectively)on the adhesive、invasive and migrative abilities of SGC7901 cells were explored by MTT and Transwell chamber. Results:(1)12G5 showed a time/dose-dependent inhibition effect on SGC7901 cells adhering Matrigel,and the adhesion of gastric cancer cells was inhibited on the concentration of 10?g/ml of 12G5, and compared with untreated group,adhesion of SGC7901 cells was signficantly lower(P

Abnormal methylation of the tumor-related genes is frequently found in the development of gastric cancer.Cancer cells exhibit two opposing methylation abnormalities:genome-wide hypomethylation and certain tumor suppressor gene hypermethylation.Furthermore,it seems that the level of DNA methylation is closely associated with the prognosis of gastric cancer.Recently,a great deal of research has been conducted to reveal the corelation of DNA methylation and gastric cancer.

The present study was to investigate whether peripheral NMDA receptors were involved in the persistent nociceptioninduced by subcutaneous (s. C. ) bee venom injection in the conscious rat by using quatitative pain scoring methods, a.c. Bee venom injection into one hindpaw resulted in a persistent, monophasic nociceptive response characterized by continuously flinching.lifting and licking the injected paw for more than I h. The non-competitive. NMDA receptor channel blockers, ketamine and ME-801, were administered s.c. 5 or 20 min after bee venom. Local ketamine injection produced a suppression of flinching reflex by 20. 90±2.88% and 45.76±13.9%, while that of lifting/bcking time by 39. 53±10. 05% and 59.94±5.53%, at doses of 25mmol/L and 50 mmol/L respectively without any motor disturbance, Local MK-801 resulted in an inhibition of flinching reflexby 22.84±3.12% and 49.53±5.35%. While that of lifting/licking time by 17. 49±5.67%and 53.49±3. 87%. At doses of 10μmol/L and 100 ,μmol/L respectively also with no motor disturbance. However, s. C. Administration of ketamine and MK-801 inior region symmetrical to the bee venom injection site on the contralateral hindpaw produced no change in the nociceptive behaviors, suggesting that the analgesic actions of keramme and MK-8Ol were not the result of systemic effects. The present resultsuggests that peripheral NMDA receptors are involved in the production of persistent pain.

Objective To evaluate rhBMP-2/20%β-TCP/PDLLA complex biological properties.Methods Preparation of casting salting was carried out using three-dimensional porous 20% rhBMP-2/β-TCP/PDLLA. 24 4-month-old healthy male New Zealand rabbits were randomly divided into A,B groups of 12,according to the method described in Boden production L4,5 intertransverse process fusion model. A group was embedding rhBMP-2/20%β-TCP/PDLLA complex,and B group was implanted autogenous iliac bone block. Animals were killed according to postoperative time(3 weeks,6 weeks, 12 weeks) ,for gross observation, X-ray observation of the formation of new bone, artificial bone degradation, and early histological manifestations. Results Composite artificial bone and autologous iliac bone graft were able to achieve good spinal fusion,artificial bone graft where there is no inflammatory response,but also in new bone formation,while reshaping the gradual degradation of carrier material. Conclusions 20%β-TCP/PDLLA showed good biocompatibility. Its structure and micro-environment created by its degradation were conducive to play a role for the rhBMP-2. β-TCP/PDLLA had strong activity in inducing bone and good bone fusion ability,and it was a promising bone graft substitutes, which fusion power was stronger than that in autologous iliac bone graft.

"Be cautious alone"is a important idea of Confucianists,it means that one should be very cautious to obey every moral standard and not to do any immoral things when he is being alone.The character of purchasing and the particularity of Medical substances demand that Medical substances buyers must be cautious when being alone."Be cautious alone"may help buyers to increase their self-discipline and arouse their vigilance to check erroneous ideas at the outset,and help them to culture a harmonious purchaseing idea.

0.05). Oil red O staining demonstrated that lipid droplet number was significantly less in the each adipogenic inductor + extracting solution from Eucommia ulmoides Oliv. groups compared with the adipogenic inductor group. CONCLUSION: Extracting solution from Eucommia ulmoides Oliv. can induce the differentiation of in vitro cultured goat BMSCs into osteoblasts, and inhibit their differentiation into adipocytes.

Object To study the chemical constituents from fruiting bodies of Ganoderma lucidum (Leyss ex Fr.) Karst Methods Individual constituent was isolated and repeatedly purified on silica gel column and structurally elucidated by the NMR spectra and X ray diffraction analysis Results Four compounds were obtained from the alcohol extract and identified as 3? oxo formyl 7?, 12? dihydroxy 4, 4, 14? trimethyl 5? chol 11, 15 dioxo 8 en (E) 24 oic acid (Ⅵ); 3? oxo formyl 7?, 12? dihydroxy 5? lanost 11, 15, 23 trioxo 8 en (E) 26 oic acid (Ⅶ); ergosterol (Ⅷ) and ergosta 7, 22 dien 3? ol (Ⅸ), respectively Conclusion Compound Ⅵ and Ⅶ are new triterpenes

Objective To detect the expression of DNA repair enzyme O6-methylguanine DNA methyl-transferase(MGMT),cell proliferation-related nuclear protein(Ki-67)and P53 protein in gliomas,and inves-tigate the relationship and clinical significance among them and gloma grade. Methods 61 cases of brain glio-ma specimes and 16 cases of internal decompression of brain trauma were used to detect the expression of MGMT,Ki-67 and P53 by using immunohistochemical SP method. Results MGMT protein expression (19. 67%∶0,χ2 =3. 729,P=0. 062),Ki-67 protein expression(39. 34% ∶0,χ2 =5. 722,P=0. 016)and P53 protein expression(27. 87% ∶0,χ2 =9. 146,P=0. 002)showed significant differences in gliomas com-pared to normal brain tissues,the expression of Ki-67 was significantly higher in high-grade gliomas(Ⅲ-Ⅳ) than that in low-grade gliomas(Ⅰ-Ⅱ)(14 ∶10,χ2 =11. 718,P =0. 001). No significant difference was found between the MGMT and P53. Conclusion MGMT protein can be used as a biomarker in gliomas detec-tion;Ki-67 has a positive correlation with tumor grade,and can be used as a reference indicator of pathological grade;P53 protein expression may be used as a potential target for the treatment of gliomas.