Acute Disease ; Child ; Humans ; Respiratory Tract Infections ; virology

China ; Diarrhea ; prevention & control ; virology ; Humans ; Viral Vaccines ; immunology ; Virus Diseases ; prevention & control

Child, Preschool ; Communicable Disease Control ; Diarrhea ; prevention & control ; virology ; Female ; Humans ; Infant ; Infant, Newborn ; Male ; Virus Diseases ; prevention & control ; virology ; Virus Physiological Phenomena ; Viruses ; isolation & purification

Objective:To study the characters of perioral muscle p re ssure of the adult patients with skeletal crossbite. Methods:20 patients were diagnosed to be with skeletal crossbite. The perioral muscle press ures on upper and lower central incisors, first molars and cuspids were measured buccally and lingually at rest position. 10 health adults were served as the co ntrols. Results:The perioral force in skeletal crossbite group was larger than that in the control group on the correspondence area(P0.05). In the skeletal cross bite group, the perioral force on the mandibular area was larger than that on th e correspondence maxillary area(P0.05). The muscle pressure on the labial side was larger than that on t he lingual correspondence side in both groups(P

Actinomycosis ; pathology ; Adult ; Diagnosis, Differential ; Female ; Humans ; Mycoses ; pathology ; Staphylococcal Skin Infections ; microbiology ; pathology ; surgery ; Staphylococcus aureus ; isolation & purification ; Young Adult

Administration, Oral ; Humans ; Rotavirus Infections ; immunology ; prevention & control ; Viral Vaccines ; administration & dosage ; immunology

Objective To analyze a large number of Cumulative Paternity Index (CPI) data in Single-parentage identification with stochastic simulation method. Methods Using the software in Identifiler, PP18D, AGCU EX20, PP21, AGCU 21+1 system, 1 million groups of STR genotyping of parent-child were analyzed. The average rate of very low CPI (RVLCPI), the median of PI in every loci (MPI), and the average PI of 39 STR loci (PIsolo) were counted out. And calculation formula of the number of STR loci that need more testing (N) was derived. Results RVLCPI in Identiifler, PP18D, AGCU EX20, PP21, AGCU 21+1 system were 24.9%, 6.5%, 1.4%, 0.7%, 0.000 1%. On the premise of inclusion relations between every kits, the number of autosome STR loci is inversely proportional to RVLCPI. PIsolo was 1.843 2. There was a signiifcant positive correlation between MPI and PEduo (r=0.956, P<0.001). The formula of the number of STR loci that need more testing was N=?log1.843 2(10 000/P)?. Conclusion The result and the formula in this paper are effcient in single-parentage identiifcation.

0.05). Conclusion: The tongue measurement with ultrasonic imaging is reproducible.

Objective To determine the effects of isoflurane on the amino acid neurotransmitter contents in rat cerebral cortex, hippocampus and spinal cord. Methods Sixteen male SD rats weighting 220-280g were randomly divided into two groups: isoflurane group (A) and control group (B). Animals in group A were killed after 30min inhalation of 1.3% isoflurane and cerebral cortex, hippocampus and spinal cord were removed immediately for determination of glutamic acid (Glu), aspartic acid (ASP), glutamine (Gln), GABA and glycine (Gly) levels by high-performance liquid chromatography (HPLC), whereas in group B O2 was inhaled instead of isoflurane. Results As compared with control group, Asp and Glu levels in cerebral cortex and hippocampus decreased markedly while Gly level increased significantly in hippocampus and spinal cord in isoflurane group. Conclusions The inhibition of excitatory amino acid synapse transmission and augmentation of inhibitory amino acid synapse transmission may be involved in the mechanism of isoflurane anesthesia.

Objective In order to further understand the bioactivity of sphingosine kinase, high efficient replication defective recombinant adenovirus carrying the wild type SPK gene (SPK WT ) and SPK mutant gene (SPK DN ) were constructed respectively. Methods The SPK genes of interest were subcloned into a shuttle vector pshuttle cmv respectively. Pshuttle cmv SPK WT and Pshuttle cmv SPK DN were linearized by PmeI and transformed respectively into the competent cells of E. coli BJ AD 1 cells which contain adenoviral backbone plasmid pAdEasy 1. The linearized recombinant plasmids were transfected into adenovirus 293 packaging cells respectively,and then recombinant adenoviruses were harvested. We performed the PCR, Western blot and enzyme assays to identify the recombinant adenoviruses. Results The recombinant adenoviruses containing the interest gene and being able to infect ECV 304 cells were obtained. Overexpression of wild type SPK gene in ECV 304 cells increased the endogenous SPK activity, whereas overexpression of mutant SPK (SPK DN ) inhibited intracellular SPK activity. Conclusion Recombinant adenoviral vectors can mediate interesting gene expression in cells.