BACKGROUND: It is thought in traditional Chinese medicine that "the head is the residence of intelligence", "the head is the leader of body, and the focus of the spirit". Therefore, scalp electro-acupuncture can induce resuscitation, relieve mental confusion, dredge the meridian and eliminate the depression in treatment of post-stroke depression.OBJECTIVE: To observe the curative effect of head acupuncture on poststroke depression.DESIGN: Randomized controlled and single blind evaluationSETTING: Department of Acupuncture and Moxibustion, Nanfang Hospital of Southern Medical University; College of Acupuncture and Massage,Guangzhou University of Traditional Chinese Medicine.PARTICIPANTS: Totally 90 patients with post-stroke depression were from Affiliated Nanfang Hospital and,Zhujiang Hospital of Southern Medical University, First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine were recruited. The Score of all the patients in Hamilton Rating Scale for Depression (HRSD) were ＞ 20. Ninety patients were randomly divided as scalp acupuncture group (n=46) and routine acupuncture group (n=44).METHODS: The patients in the routine acupuncture group were treated mainly at points of three-yang meridians of foot and hand at the hemiplegia side. The points include jianyu (LI-15) , binao (LI-14) , quchi (LI-11),shousanli (LI-10), waiguan (SJ-5), hegu (LI-4), biguan (St-31), liangqiu (St-34), zusanli (St-36), yanglingquan (GB-34), kunlun (UB-60), jiexi (St41). The above points were divided into 2 groups, 1 group each day, alternately. Even reinforcing-reducing method was used, with 30-minute needle retention, 6 successive days treatment with 1 day rest, for 6 weeks totally.The patients in the scalp acupuncture were treated with, besides the above method, scalp acupuncture. Mid-parietal line, mid-frontal line, laterofrontal lineI (both sides)were chosen. After arrival of qi, G6805 type electro-acupuncture apparatus (interrupted wave, 50 Hz,4 V) was connected, with 30-minute needle retention, 6 suocessive days treatment with 1 day rest, 6 for 6 weeks totally. HRSD was used to evaluate the status of depression before and after treatment. Stroke score was used to evaluate the curative effect of stroke.MAIN OUTCOME MEASURES: HRSD scores and curative effect of apoplexy before and after treatment of the patients in two groups.RESULTS: According to the intention treatment, all 90 patients entered the result analysis. ① HRSD score after treatment was lower than that be fore treatment in the scalp acupuncture group,and that after treatment group in the routine acupuncture group (8.55±1.73,30.61±4.02,15.04±2.33,P ＜ 0.01 ). ②There was no significant difference of the total effective rate in the two groups , but some tendency indicated the effective rate in the scalp acupuncture group was better than that in the routine acupuncture group ( 100% ,98% ,x2=1.9167 ,P ＞ 0.05).CONCLUSION: Scalp electro-acupuncture has obvious curative effect on post-stroke depression. The amelioration of melancholia has a certain promoting effect on rehabilitation of apoplexy.

Objective: Observe the curative effect of basic recipes for removing phlegm and activating blood circulation in experimental model of non-alcoholic fatty liver in rats.Methods: Experimental animal model of non-alcoholic fatty liver in rats were established,then treated with Erchen decoction,TaohongSiwu decoction and the combination decoction.Basic biochemical indexes of rats were tested after treatment.Results: Erchen decoction can reduce the level of liver organ coefficient,cholesterin and aspartate aminotransferase(AST)(P

Objective To design an experimental model of endoleak after endovascular exclusion(EVE). Methods Infrarenal aortic aneurysms were created with bovine jugular vein segements or patches. Then they were undergone endovascular stent graft exclusion of the aneurysm. Using modification of prosthetic vessel and changing the attachment site, endoleaks were formed druing the course of aneurysm exclusion. Results All the 6 aneurysms possessed satisfactory configuration just as clinical patterns. Intraoperative arteriography revealed endoleaks in 5 dogs after the exclusion, two of which were proximal and three were distal.Conclusions This experiment shows the hemodynamics and treatment of endoleak for EVE.

Objective To investigate the hemodynamics and structural effect of rat endothelial progenitor cells (EPC) transplant on pulmonary artery hypertension (PAH) induced by monocrotaline(MCT) in rats.Methods EPCs were identified and marked.Twenty-one days after injection of EPCs,the pulmonary hemodynamic parame- ters,average pulmonary artery pressure (mPAP),right heart index were determined.The vascular endothelial cells and pulmonary vascular structural changes were verified by fluoresccuse microscope.Results Compared with the model,EPCs treatment(n=10) decreased mPAP significantly (mPAP,EPCs:25.9?0.7 mmHg vs model group:29.3?2.2 mmHg,P

Objective To evaluate the role of TCD examination in diagnosis of the early cerebrovascular diseases in aged diabetic patients.Methods 179 cases of aged type Ⅱ diabetic patients were divided into three groups(PDR,BDR and NDB)according to their retinopathy.TCD and retina examination were performed in all patients and the data be analysed. Results (1)The systolic peak flow velocity(Vp) of MCA、ACA、ICA and BA were significant increased in diabetic pa- tients than in normal control group(P

Acute Kidney Injury ; etiology ; Hemolytic-Uremic Syndrome ; complications ; etiology ; therapy ; Humans ; Shiga Toxin ; toxicity

Objective To analyze the risk factors associated with allergic disease in preterm infants during the first year of life. To investigate whether there was any association between peripheral blood eosinophils and allergic di-sease during the first year of life benefit of hydrolyzed formula feeding in preterm infants. Methods A nested case -control study was conducted in a cohort of artificial feeding preterm infants admitted to Neonatal Intensive Care Unit, Shanghai Children's Medical Center from January 2012 to April 2013. The preterm infants were monitored from birth up to 1 year of age and the findings were related to development of allergic disease. Individuals with allergic disease during the first year of life(cases)were matched with gender,as previously described with two individuals(controls)who re-mained event - free during the study. The risk factors associated with allergic disease in preterm infants during the first year of life were analyzed. And whether there was any association between peripheral blood eosinophils and allergic di-sease during the first year of life benefit of a hydrolyzed formula feeding in preterm infants was investigated. Different variables in control and case individuals were compared with t test for normal distribution measurement date,χ2 tests for categorical variables and Mann - Whitney U test for non - normal distribution measurement date,and multiple conditio-nal Logistic regression were used to investigate the risk factors associated with allergic disease in preterm infants. Results Thirty - four individuals were in cases and 68 individuals were in controls. In a conditional multivariable Logistic model,peripheral blood eosinophils of preterm infants at full enteral feeding(EOS - 2)(OR = 5. 941,95% CI:1. 165 - 41. 375,P ﹤ 0. 05),and family history of allergy(OR = 3. 316,95% CI:1. 201 - 9. 152,P ﹤ 0. 05)were the two independent risk factors for allergic disease in preterm infants during the first year of life. Individuals fed with standard preterm formula after birth,the association between EOS - 2 and allergic disease was significantly enhanced (OR = 21. 459,95% CI:1. 686 - 273. 152,P ﹤ 0. 05). By contrast,in individuals fed with hydrolyzed formula,the risk of EOS - 2 was substantially attenuated(OR = 1. 708,95% CI:0. 148 - 19. 743,P ﹥ 0. 05). Conclusions Peripheral blood eosinophils of preterm infants at full enteral feeding EOS - 2 and family history of allergy were the 2 independent risk factors for allergic disease in preterm infants during the first year of life. Contrast to individuals fed with standard preterm formula after birth,individuals fed with hydrolyzed formula had lower association between factors of peripheral blood eosinophils and family history of allergy,and allergic disease during the first year of life.

Objective To evaluate left atrial function dynamic changes before and after radiofrequency catheter ablation (CA)with persistent atrial fibrillation (AF)and predict the maintain sinus rhythm of postoperative atrial fibrillation by speckle tracking imaging.Methods The study was including 45 cases underwent CA with persistent AF(preoperative 1 week,postoperative 24 hours,3 months,6 months)and 25 normal cases.Left atrial function and strain rate were analysed.These index were measured and calculated:the left atrial diameter index (LADI),left atrial area index (LAAI),left atrial maximum volume index (LAVImax)and left atrial ejection fraction(LAEF),left atrial wall strain rate of left ventricular systolie (SRs),left atrial wall strain rate of left ventricular early diastolie (SRe),left atrial systolic strain rate(SRa) and the average of left atrial wall each phase strain rate.Results ① LADI,LAAI,LAVImax were significantly increased;LAEF,SRs,SRe were decreased significantly;SRa were disappeared with AF compared with the control group.②LADI,LAAI,LAVImax decreased gradually,LAEF,SRs,SRe,SRa were gradually increased with the extension of the follow-up time.③Left atrial average SRs predicted maintenance of sinus rhythm with atrial fibrillation after operation of CA(odds ratio=0.607,95% CI=0.417-0.819,correction of P =0.017)was an independent predictor.Conclusions Speckle tracking imaging can quantitative evaluate left atrial function with persistent AF after CA.Left atrial global reservoir function predicts the efficacy of CA.

Objective To explore the curative effect of ursodeoxycholi acid on retinopathy of prematurity (ROP) caused by high concentration of oxygen in newborn rats. Methods The model of ROP was established. Neonatal rats were divided into normal control group, ROP model group, low dose ursodeoxycholi acid treatment group (10mg/kg) and high dose ursodeoxycholi acid treatment group (40mg/kg). Rats were sacrificed at days 17. The new retinal vessels were observed and counted under lfuorescence microscope. Results The new retinal vessels in ROP rats were hyperplastic, twisted and unevenly distributed. There was signiifcant difference in the number of new retinal vessels among different groups (P=0.000). The number of new retinal vessels of rats in ROP group, low-dose group and high-dose group was signiifcantly more than that in control group (P=0.000). The number of new retinal vessels in low-dose group and high-dose group was significantly less than that in ROP group (P<0.05). The number of new retinal vessels in high-dose group was less than that in low-dose group (P>0.05). Conclusions Ursodeoxycholi acid could inhibit the angiogenesis of retina and could have curative effect on ROP.

BACKGROUND: Wnt signaling pathways can stimulate the proliferation and differentiation of neural stem cel s, and promote the repair of spinal cord injury. The electrical field stimulation can change the protein expression of Wnt signaling pathways.
QBJECTIVE: To explore the influence of oscil ating electric field on motor function recovery and the expression of Wnt-3a protein in rats with spinal cord injury.
METHODS: A total of 36 Sprague-Dawley rats models with spinal cord injury were induced by the Al en’s method. Al rats were randomly divided into oscil ating electric field stimulation group and spinal cord injury group. The stimulating electrodes were added in both groups. The oscil ating electric field stimulation group received oscil ating electric field intervention.
RESULTS AND CONCLUSION: At 3 days after model establishment, Basso, Beattie, and Bresnahan (BBB) locomotor rating scale scores and Wnt-3a expression were similar between the oscil ating electric field stimulation group and spinal cord injury group. At 7 and 14 days, significant differences in BBB scores and Wnt-3a expression were detected. These results indicate that oscil ating electric field stimulation can activate Wnt signaling protein in the early stage of spinal cord injury, which may be associated with the promoting effect of oscil ating electric field stimulation on spinal cord injury.