No abstract available.
Humans ; Humerus* ; Neck* ; Shoulder Dislocation* ; Shoulder* ; Tears*

A 42-year-old male patient presented with severe periodontal disease requiring extensive vertical bone augmentation (VBA) prior to implant placement. Following tooth extraction and periodontal treatment, VBA was achieved using a xenogeneic graft and stabilized with a native bilayer collagen membrane and fixation screw. Owing to the limited volume maintenance of the resorbable membrane, the implant was installed with reinforcement using a sinus crestal approach. Over an 8-year follow-up period, the implant exhibited stable alveolar bone without signs of peri-implantitis or significant bone resorption, suggesting the efficacy and longevity of this technique.

A 42-year-old male patient presented with severe periodontal disease requiring extensive vertical bone augmentation (VBA) prior to implant placement. Following tooth extraction and periodontal treatment, VBA was achieved using a xenogeneic graft and stabilized with a native bilayer collagen membrane and fixation screw. Owing to the limited volume maintenance of the resorbable membrane, the implant was installed with reinforcement using a sinus crestal approach. Over an 8-year follow-up period, the implant exhibited stable alveolar bone without signs of peri-implantitis or significant bone resorption, suggesting the efficacy and longevity of this technique.

A 42-year-old male patient presented with severe periodontal disease requiring extensive vertical bone augmentation (VBA) prior to implant placement. Following tooth extraction and periodontal treatment, VBA was achieved using a xenogeneic graft and stabilized with a native bilayer collagen membrane and fixation screw. Owing to the limited volume maintenance of the resorbable membrane, the implant was installed with reinforcement using a sinus crestal approach. Over an 8-year follow-up period, the implant exhibited stable alveolar bone without signs of peri-implantitis or significant bone resorption, suggesting the efficacy and longevity of this technique.

PURPOSE: Continuous intravenous access is imperative in emergency situations. Ultrasound-guided internal jugular vein (IJV) catheterization was investigated in critically ill pediatric patients to assess the feasibility of the procedure. METHODS: Patients admitted to the pediatric intensive care unit between February 2011 and September 2012 were enrolled in this study. All patients received a central venous catheter from attending house staff under ultrasound guidance. Outcome measures included successful insertion of the catheter, cannulation time, number of cannulation attempts, and number and type of resulting complications. RESULTS: Forty-one central venous catheters (93.2%) were successfully inserted into 44 patients (21 males and 23 females; mean age, 6.54+/-1.06 years). Thirty-three patients (75.0%) had neurological disorders. The right IJV was used for catheter insertion in 34 cases (82.9%). The mean number of cannulation attempts and the mean cannulation time was 1.57+/-0.34 and 14.07+/-1.91 minutes, respectively, the mean catheter dwell time was 14.73+/-2.5 days. Accidental catheter removal was observed in 9 patients (22.0%). Six patients (13.6%) reported complications, the most serious being catheter-related sepsis, which affected 1 patient (2.3%). Other complications included 2 reported cases of catheter malposition (4.6%), and 1 case each of arterial puncture (2.3%), pneumothorax (2.3%), and skin infection (2.3%). CONCLUSION: The results suggest that ultrasound-guided IJV catheterization can be performed easily and without any serious complications in pediatric patients, even when performed by visiting house staff. Therefore, ultrasound-guided IJV catheterization is strongly recommended for critically ill pediatric patients.
Catheterization* ; Catheters* ; Central Venous Catheters ; Child ; Critical Illness* ; Emergencies ; Female ; Humans ; Intensive Care Units ; Internship and Residency ; Jugular Veins* ; Male ; Nervous System Diseases ; Outcome Assessment (Health Care) ; Pneumothorax ; Punctures ; Sepsis ; Skin ; Ultrasonography

PURPOSE: Features of epidermal growth factor receptor (EGFR) expression in osteosarcoma and in vitro efficacies of EGFR inhibitors against osteosarcoma cells were evaluated. MATERIALS AND METHODS: Thirty biopsy samples of osteosarcoma patients were retrospectively analyzed for EGFR protein expression by immunohistochemistry. Relationships between EGFR expression and clinicopathologic characteristics and treatment outcomes were evaluated. Four osteosarcoma cell lines were analyzed for EGFR and p-EGFR expression by western blotting. Efficacies of gefitinib and BIBW2992 on osteosarcoma cells were evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Tyrosine kinase domains in exons 18 to 21 were sequenced and gene expression analyses of EGFR and PTEN were performed in four osteosarcoma cell lines. RESULTS: EGFR protein was expressed in 27 (90%) samples (6 low, 12 intermediate, 9 high) and in three cell lines. Intermediate or high staining for EGFR was related to a tumor volume<150 mL (p<0.001) and histologic subtype other than osteoblastic type (p=0.03). However, EGFR expression was not associated with histologic response to preoperative chemotherapy or survival. Gefitinib and BIBW 2992 did not have any significant inhibitory effect on cell viabilities. DNA sequencing analysis revealed three osteosarcoma cell lines have single base changes at codon 2361 of exon 20 (G to A), without affecting translation results. Furthermore, no mutation was found to be associated with constitutive EGFR activation. CONCLUSION: In the present study, gefitinib and BIBW2992 were not effective against osteosarcoma cells. However, as osteosarcoma cells express EGFR, further studies are necessary to explore the potential of other therapeutic agents targeting EGFR.
Biopsy ; Blotting, Western ; Cell Line ; Cell Survival ; Codon ; Epidermal Growth Factor ; Exons ; Gene Expression ; Humans ; Immunohistochemistry ; Osteoblasts ; Osteosarcoma ; Protein-Tyrosine Kinases ; Quinazolines ; Receptor, Epidermal Growth Factor ; Retrospective Studies ; Sequence Analysis, DNA ; Tetrazolium Salts ; Thiazoles

OBJECTIVE: To investigate the real-time cardiovascular response to the progressive overload exercise in different levels of spinal cord injury (SCI), and to find out whether regular exercise has effect on these cardiovascular responses. METHODS: The study enrolled 8 able-bodied individuals in the control group plus 15 SCI subjects who were divided into two groups by their neurological level of injury: high-level SCI group (T6 or above) and low-level SCI group (T7 or below). Also, subjects were divided into exercise group and non-exercise group by usual exercise habits. We instructed the subjects to perform exercises using arm ergometer according to the protocol and checked plethysmograph for the real time assessment of blood pressure, heart rate, and cardiac output. RESULTS: Six subjects were included in high-level SCI group (3 cervical, 3 thoracic injuries), 9 subjects in low-level SCI group (9 thoracic injuries), and 8 able-bodied individuals in control group. During arm ergometer-graded exercise, mean arterial pressure (MAP) was significantly lower in high-level SCI subjects of non-exercise group, compared with high-level SCI subjects of exercise group. In addition, HR was significantly higher in low-level SCI group compared with control group. CONCLUSION: There are significant differences in mean arterial pressure of high-level SCI group according to usual exercise habits. We discovered that even in non-athlete high-level SCI, regular exercise can bring cardiac modulation through blood pressure control.
Arm ; Arterial Pressure ; Blood Pressure ; Cardiac Output ; Cardiovascular Physiological Phenomena ; Exercise ; Exercise Test ; Heart Rate ; Hemodynamics* ; Spinal Cord Injuries* ; Spinal Cord*

To identify the presence of inwardly rectifying K+ channels and its characteristics, membrane currents were measured using a whole-cell patch clamp from isolated gastric myocytes of guinea-pig. Change of external K+ concentration from 5 to 90 mM induced an inward current at a holding potential of 80 mV. The high K+-induced inward current was blocked by Ba2+ and Cs+, but not by glibenclamide. With 90 mM K+ in bath, the Ba2+- and Cs+-sensitive currents showed strong inward rectification. Ten mM TEA weakly blocked the inward current only at potentials more negative than 50 mV. With 90 mM K+ in bath, hyperpolarizing step pulses from 10 mV induced inward currents, which were inactivated at potentials more negative than 70 mV. Reduction of external K+ to 60 mM decreased the amplitudes of the currents and shifted the reversal potential to more negative potential. The inactivation of inward K+ current at negative clamp voltage was not affected by removing external Na . These results suggest that the inwardly rectifying K+ channels may exist in gastric smooth muscle.
Baths ; Glyburide ; Membranes ; Muscle Cells* ; Muscle, Smooth ; Tea

BACKGROUND AND OBJECTIVES: Performing oncologically safe, wide surgical excisions and postoperative volumetric restoration of resected tongues to gain functional recovery are contrary problems to deal with in performing glossectomy. There is no report about the volumetric restoration of a resected tongue according to resecting devices until now. The purpose of this study is to clarify the difference of postoperative volumetric changes of a resected tongue using a scalpel, electrocautery and laser with or without closure. MATERIALS AND METHOD: We used 12 rabbits for each group according to the resecting devices and also equally divided each group according to the closure or non-closure method. The volumetric changes of a resected tongue were measured by the Archimedes principles in one, two, three, and six months postoperatively. RESULTS: Regardless of resecting devices, the postoperative volume of a resected tongue was gradually increased as elapsed time. The nonclosure method was better than the closure method with respect to the volumetric restoration of the resected tongue regardless of the resecting devices, but there was no statistical significance of volumetric restoration between the closure and nonclosure methods in each group (p>0.05). The superior resecting device for the volumetric restoration of a resected tongue was diode laser, especially compared to electrocautry, in both closure and nonclosure method (p<0.05). CONCLUSION: The volumetric restoration after resection of the tongue using laser and nonclosure method are superior to electrocautery and to the closure method, respectively.
Electrocoagulation* ; Glossectomy ; Lasers, Semiconductor* ; Rabbits ; Tongue*

Purpose: Bilateral axillo-breast approach robotic parathyroidectomy (BABA-RP) aims to remove overactive or enlarged parathyroid glands with no visible neck collar incision. In this study, we compared the safety and surgical outcomes of BABA-RP vs. those of an open surgery group to ascertain whether BABA-RP is a safe and feasible surgical approach for patients with primary hyperparathyroidism (pHPT). Methods: This single-institution retrospective cohort study included 74 patients with primary HPT who underwent open parathyroidectomy (n = 37) or BABA-RP (n = 37) at our institution between November 2014 and March 2023. Patient demographics, biochemical cure rates, operative time, blood loss rates, and complication rates were examined and compared. Results: The patients in the BABA-RP group were younger and had a longer mean operative time. Regarding complication events, 2 patients in the open surgery group and 1 patient in the BABA-RP group had transient hypoparathyroidism. All 74 patients achieved biochemical cure at <6 months, regardless of the approach used. Two patients in the BABA-RP group and 1 patient in the open surgery group had carcinoma on surgical pathology. All 3 patients with parathyroid carcinoma remained recurrence-free at 1-year follow-up. Conclusion Compared with the open procedure, BABA-RP is a safe and feasible procedure that provides an excellent biochemical cure rate for patients with pHPT and has superior cosmetic benefits with equivalent surgical outcomes.