Objectives:To observe the changes in metabolism of fatty acids in breast cancer cells and the effects of inhibiting fatty acid synthase(FAS) on the growth of breast cancer cells. Methods:By RT PCR,the expression of FAS mRNA in breast cancer cell line was examined.The growth inhibition of breast cancer cells by specific FAS inhibitor, cerulenin, was determined by MTT assay.Flow cytometric analysis was used to study the changes of cell cycle. Results:FAS mRNA expression in MCF 7 cells was high.We found that cerulenin caused dose and time dependent inhibition of growth of MCF 7 cells.The growth inhibition of MCF 7 after 48 hours exposure of cerulenin at 2.5,5,10 and 20 mg/L was ( 43.47 ?4.58)%,(62.92?2.68)%,(81.93?0.91)% and (67.7?12.27)%( P

Objective: To evaluate the effects of enteral nutrition(EN) containing dietary fiber and parenteral nutrition(PN) on nutritional status and immune function of patients following surgery for carcinoma of stomach.Methods: 60 patients who underwent surgery for carcinoma of stomach were randomized to PN group(n=20),Pepti-2000(elemental diet) group(n=20) or Nutrison Multi Fibre(EN containing dietary fiber) group(n=20) for 7 days.Isocaloric and isonitrogenous nutritional supports began at the first day after operation and were maintained for a week.Nutritional variables such as body weight,serum albumin,transferin and accumulated nitrogen balance were measured preoperatively and on the 8th day after operation.Meanwhile,immune variables such as peripheral lymphocytes counts,subsets of lymphocytes(CD3,CD4,CD8,CD25),NK cells,serum immunoglobulins(IgG,IgM,IgA),complement system(C3,C4,CH50) and C-reactive protein(CRP) were also determined.Results: No serious complications occurred in all groups after surgery.Body weight decreased significantly in all groups(P

Adolescent ; Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Poisoning ; complications ; Rhabdomyolysis ; chemically induced ; Young Adult

Air Pollutants ; toxicity ; Air Pollution, Indoor ; adverse effects ; Animals ; DNA Damage ; drug effects ; Female ; Interior Design and Furnishings ; Male ; Mice ; Mutagenicity Tests

Objectives: To observe the effects and the mechanisms of exogenous fatty acids on colorectal cancer cells(LoVo). Methods:The effect of exogenous fatty acids on LoVo cells was determined by 5 diphenyl tetrazolium bromide(MTT) assay.The content of MDA was examined to evaluate the extent of lipid peroxidation. Results:The growth of LoVo cells was inhibited by polyunsaturated fatty acids(PUFAs).The inhibitory effect of saturated fatty acids and monounsaturated fatty acids on LoVo cells was not showed.None of the fatty acids was found to fibroblast cells(HLF).PUFAs caused the significant rising of intracellular MDA content. Conclusions:PUFAs could inhibit the growth of colorectal cancer cells.The strengthening of lipid peroxidation may be one of the mechanisms.

Objective To investigate the effect of excessive fluoride,aluminum on Zn,Fe,Ca,Mg,Cuin rat blood.Methods Forty eight SD rats were randomly divided into 4 groups matched with their weights:control group,high aluminum group,high fluorine group and high fluorine-aluminum group.Aluminum content in their drinking water was 0,90,0,90 mg/L respectively.Fluorine content of their feed was 5.2,5.2,106.0,106.0 mg/kg and aluminum Was 6.8,6.8,19.7,19.7 mg/kg respectively.90 days later,the level of blood Zn,Fe,Ca,Mg, Cu Was detected by the atomic absorption spectrometry.Results Compared among these groups,Zn,Fe,Mg and Cu content of the whole blood had significant difierences(F=46.25,14.74,6.10,2.93,P<0.05),while Ca content of the whole blood did not significantly change(F=2.81.P>0.05).Factorial analysis showed that excessive intake of aluminum could significantly decreased Zn,Fe,Mg content of the blood(F=42.66,5.41,7.04,P<0.05)and excessive intake of fluorine could significantly decreased Zn,Fe,Mg,Cu content of the blood(F=64.50,37.90,9.75,6.74, P<0.05).The coexistence offluorine and Muminum had interaction to the level of Zn(F=31.59,P<0.05)and did not obviously interact with other elements(F=0.91,1.63,1.51.0.00,P>0.05).Compared with the control group [(131.30 ±13.86)μmol/L,(10.24 ±1.02),(1.71 ±0.19)mmol/L,(20.43 ±4.42)μmol/L],Zn content in the high aluminum group[(90.84±9.98)μmol/L]decreased significantly(P<0.05),so did Zn,Fe,Mg content in tlle high fluorine group[(85.85 ±10.92)μmol/L,(8.49 ±0.68),(1.52 ±0.13)mmol/L],the difference being statistically significant(P<0.05)0Zn,Fe,Mg,Cu content in the high fluorine-aluminum group,being(82.82 ±11.00)μmol/L, (8.16±0.45),(1.46±0.09)mmoL/L,(15.69±2.38)μmol/L,respectively,all decreased signitlcarIdy(P<0.05). Compared with the high aluminum group[(9.43±1.09)mmol/L],Fe content of the high fluorine aluminum group[(8.16±0.45)mmol/L]decreased significantly(P<0.05).Conclusions Excessive fluoride can cause blood zn, Fe,Mg,Cu decline,so can excessive aluminum.Combination of excessive fluofine and aiuminum has 8ignificant synergic effect on the level of Zn but have rio influence on Ca.

In this paper, an analysis was made on the varieties and standards of labiatae medicinal plants used in Tibetan medicine. The results showed 71 species of labiatae plants in 21 genera (including varieties) recorded in relevant literatures, involving 44 varieties of medicinal materials. Specifically, seven species (9.9%) were intersected with traditional Chinese medicines (TCM), 19 varieties (43%) were recorded in Chinese medicinal material standards at all levels, and 27 species (38%) were source plants. In Tibetan medicine standards and literatures, there are great differences between Tibetan names and translated Chinese names and among varieties of source plants. Apart from a few of varieties intersected with traditional Chinese medicines had complete standards and regulations in Chinese Pharmacopoeia, most of species only had characters, microscopic, physical and chemical identifications in Standards Issued by Ministry of Health-Tibetan Medicine, Tibetan Medicine Standard and local standards. Therefore, the Tibetan medicinal material variety-source specification and quality standard system shall be promoted on the basis of literatures research, investigations for resources and current applications and modern pharmaceutical studies.
Drugs, Chinese Herbal ; chemistry ; pharmacology ; Humans ; Lamiaceae ; chemistry ; classification ; Medicine, Tibetan Traditional ; standards ; Phytotherapy ; standards ; Plants, Medicinal ; chemistry ; classification

Objective:To explore the operative indications, methods, curative effects and clinical prospect of Endovascular Graft Exclusion (EVGE) for descending thoracic aortic dissection (TAD). Methods:Under dynamic supervision of DSA, an EVGE with a stent graft complex of 130 44 mm was performed successfully on a 75 year old man with an aortic dissection (DeBakey ⅢB type). Results:Following the operation, DSA revealed that the intimal tear of the dissection was excluded by the endoluminal stent graft complex and the false lumen disappeared. The postoperative one week and one month, Duplex scan and CTA revealed that the stent graft complex was patent and had an inner diameter of 36 mm, without any migration and torsion. The false lumen of dissection was full of thrombi. The patient was discharged in a week. One month postoperative follow up showed that the patient was recovered to his usual normality. Conclusion:The indications for EVGE are DeBakey ⅢB type aortic dissections with the intimal tear, EVGE is a minus invasive method with a firmly curative effects, its clinical prospect will be very bright.

Objective To study the correlation between obesity and clustering of major risk factors on cardiovascular disease in the elderly.Methods A cross-sectionai study was conducted.Data from 546 elderlY aged 60-90 were analyzed for the relationship between obesity and clustering of risk factors for cardiovascular diseases,with descriptive statistical methods,x2 test,logistie regression and ROC curve．Results (1)The positive rates of the four major risk factors and clustering appeared an upward trend along with the BMI and or the wc.The overall positive rate of clustering of risk factors among the total 546 elderly people was 59.5％,and 52.5％in males,65.7％in females.(2)As compared with females,males had a higher growth rate of clustering with the elevating of BMI and WC.and odds ratios of the highest-level groups were 13.579 and 2.876 in males and females.Respectively.(3)Both BMI and WC were independent influencing factors for male(P<0.05),and logistic regression coefficients were 0.196 and 0.074 for BMI and WC,respectively.Only WC appeared to be the independent influencing factor for females(P<0.05),while BMI WaS not(P:0.537),with logistic regression coefficient as 0.060 for WC.(4)The combination equation of male's BMI and WC Was CEBMI+WC=0.726 × BMI+0.274 × WC,with the optimum cut-off value as 41.59,and the sensitivity and the specificity were 0.785 and 0.598,respectively．Conclusion Four major risk factors for cardiovascular diseases were often related to obesity while control of BMI and WC were of great significance to the primary prevention of cardiovascular disease．The combined equation of BMI and WC could be used to predict the risk of clustering.

Objective To explore the relationship between types of social support and roles of supporters,on people living with HIV/AIDS,in rural areas of Henan province.Methods A rural area from Henan province where the main route of HIV transmission was through blood collection was selected as the research site.Survivors registered in that area were randomly selected as subjects.Questionnaire on social support related to social network analysis paradigm was designed and face-to-face interview was used to collect information.Correspondence analysis method was adopted to analyze the relationship between types of social support and roles of social supporters.Results 204 questionnaires were sorted out with 2227 pairs of bind between types of social support and roles of social supporters analyzed.According to scatter plot of row and column points,our data showed that support from the spouses was mainly associated with caring for daily life and companionship for medical treatment on the patients.The research subjects stated that they would primarily discuss over the major issues or chat with their parents and children as they were the ones that they could trust the most.However,they would turn to their brothers,sisters or other relatives to borrow money or asking for other kinds of help.Non-relatives were the resources on social interaction,like going-out together or borrowing life necessities.Conclusion Supporters with different social roles on HIV/AIDS issues,appeared to be corresponded to specific types of social support in rural areas of Henan province.