Objective To investigate the therapeutic role of hyperbaric oxygen(HBO) on neonatal hearing impairment induced by hypoxic-ischemic encephalopathy(HIE),by observing the brain stem auditory evoked potential(BAEP) changes of newborn infants with HIE.Methods One hundred and six cases of HIE in newborn infants(in which 82 cases were mild and 24 cases were severe) treated with HBO and conventional therapy were chosen as HBO treatment group,while other 42 cases of HIE in newborn infants(in which 38 cases were mild and 4 cases were severe) treated with conventional therapy only were served as control group.BAEP was measured at the 3rd to 7th day on patients before treatment and 42 days after birth with or without HBO therapy respectively,and the differences of BAEP changes were compared between the both groups.Results 1.In control group,the rate of abnormality was 47.4% and 31.6%,respectively before and after therapy in mild HIE cases(P=0.240),and 75.0%,50.0% in the moderate-severe HIE cases(P=0.462).2.In HBO group,the abnormal rate was 45.1% and 25.6%,respectively in mild HIE cases before and after therapy,while 75.0% and 41.7% in moderate-severe HIE cases(Pa=0.014,0.039).3.There was obvious difference between control group and HBO group in mild HIE cases(P=0.013),while there was no significant change between the 2 groups in moderate-severe HIE cases(P=0.717).Conclusions Treatment with HBO combined with conventional therapy may improve the abnormal rate of BAEP in patients with HIE according to the present test,especially in mild HIE cases,thus earlier HBO therapy may be used to mitigate hearing impairment and improve prognosis induced by the disease.

Objective To study the changes and the significance of the frangible lymphocyte in patients with fulminant viral hepatitis and investigate the relationship between the degree of frangible lymphocyte and the severe of fulminant viral hepatitis. Methods 32 cases confirmed with fulminant viral hepatitis, 66 cases with chronic hepatitis have been studied. 15 cases children from 3 to 5 years old and 46 cases adults have been used with control groups. 100?l lymphocytes (1?106/ml) and 100?l tumor cells(1?105/ml) mixed with 100?l self-serum at 37℃ for 5min to 30min. The fragmental lymphocyte can defined as a frangible lymphocyte. The lymphocyte attached with tumor cell is undestroyed anything can be regarded as negative frangible lymphocyte. Less than 30 percent of lymphocyte attached with tumor cells is destroyed will be conformed as low-grade frangible lymphocyte, between 30 percent to 60 percent regard as mid-grade, and more than 60 percent as severe-grade mode. Results The equiform frangible lymphocyte is detected in fulminant viral hepatitis and other groups like that in SARS in 2003 years. There is none of children have frangible lymphocyte. Only 3 person’s lymphocytes show severe-grade frangible and 9 people display mid-grade in 46 adult individuals. Contrarily There is high of 46.9 percent of cases with fulminant viral hepatitis show severe-grade frangible lymphocytes, only 4 cases is negative and 3 cases is recovered in one months of them. The frangible lymphocytes in fulminant viral hepatitis are significantly lower than those in chronic hepatitis patients not only in mid-grade frangible but in severe type. There is no significant recovery of frangible lymphocyte from acute to recover in fulminant viral hepatitis, but the degree of the lymphocytes are closely relate to the state of an illness. Conclusion The percent of frangible lymphocytes in children is lowest among adult people, chronic hepatitis and fulminant viral hepatitis. The proportion of frangible lymphocytes in fulminant viral hepatitis is highest, and significantly higher than that in chronic hepatitis. The phenomenon of frangible lymphocytes may be an important characteristic of an innate immunology reaction of the body.

Objective To determine the effects of different excipients ( amino acids, carbohydrates and nonionic surfactants) on thermal stability of the IgG1 monoclonal antibody, and to examine the interactions between the excipients and the protein.Methods Differential scanning calorimetry ( DSC) was used to study thermal stability of the protein in different solutions and got information on the solubility of the unfolded forms of the protein.Isothermal titration calorimetry ( ITC) was used to examine the binding interactions between the excipients and the protein.ResuIts Negatively charged amino acids could significantly reduce the denaturation temperature (Tm) of IgG1( Tm >9 ℃), and other excipients didn’t have a major effect ( Tm <1℃).Excipients shared different impacts on thermal stability of the IgG1 monoclonal antibody under different pH, and negatively charged amino acids result in a much lower Tm at pH 5 than at pH 7.The ITC binding isotherms of different excipients (including polysorbate 20 and 80) and IgG1 were almost straight lines, while there was strong binding interaction between polysorbate 20 or 80 and Human Serum Albumin (HSA).ConcIusion The results suggest that there is no binding interaction between these studied excipients and the IgG1 monoclonal antibody; instead electrostatic interactions seem to play a leading role between the excipients and the IgG1 monoclonal antibody.

common TiO2 by the comparison of these catalysts. Conclusion Catalysed by ultrasonic wave, a series of TiO2 catalysts will show a significant effect of killing colibacillus.

Objective To explore the role of platelet activating factor(PAF) and its receptor in portal hypertension in liver cirrhosis. Methods A model of hepatic cirrhosis was replicated in rat by intraperitoneal injection of CCL 4 for 8 weeks. The blood and hepatic PAF and PAF receptors contents were assayed with ELISA, RT-PCR and saturation binding technique. Results Compared with control rats, cirrhotic rats had higher hepatic PAF levels, hepatic PAF output, and plasma PAF levels, which were increased by 44%, 87.7% and 54.5%(P

Objective To evaluate the value of Univent tube in the airway management during one-lung ventilation. Methods A total of 40 patients with pneumothorax undergoing video-assisted thoracoscopic surgery (VATS) were randomly divided into Univent (U) and double-lumen tube (Robertshaw) (D) groups (20 in each). In U group, intubation was performed under the guidance of a fibreoptic bronchoscope; while in D group, the position of the tube was confirmed using fibreoptic bronchoscopy after intubation. The time of intubation, number of secondary dislodgements, quality of lung deflation, peak airway pressure during one-lung ventilation, and blood artery gas analysis data in the two groups were recorded 30 minutes after one-lung ventilation. Results The intubation time in U group was significantly longer than that in D group [(6.18?1.26) min vs (3.26?0.82) min, t=8.654, P=0.000]. The number of secondary dislodgements was 7 in U group, and 5 in D group(U=187.500, P=0.663). No significant difference was detected in the quality of lung deflation between the two groups, (U group: 15 excellent, 3 fair, and 2 poor; D group: 17 excellent, 2 fair, and 1 poor;U=179.500, P=0.583). The peak airway pressure during one-lung ventilation in U group was significantly lower than that in D group [(15.3?3.5) cm H2O vs (21.4?6.6) cm H2O, t=-3.649, P=0.001]; and the oxygen pressure in group U was significantly higher than that in group D [(303.8?65.7) mm Hg vs (258.4?72.9) mm Hg, t=2.066, P=0.046]. No significant difference was found in carbon dioxide pressure and oxygen saturation between the two groups. Conclusion During VATS, univent tube showed the same effectiveness and safety as DLT for one-lung ventilation.

Objective To explore clinical feature and coronary artery lesion characteristics of non-ST-segment elevation myocardial infarction (NSTEMI).Methods Seventy-three patients of NSTEMI were studied,their clinical data and results of coronary angiography being analyzed.Results Risk factors of coronary artery disease among all the patients were 50(68%) with hypertension,26(35%) with diabetes,34(46%) with hyperlipidemia and 28(38%) with smoking history.There were 52(71%)with angina pectoris before this hospitalization,22(30.6%) with inhospatial angina pectoris,15(20.4%) with severe arrythmias,7(10.2%) with cardiac shock,3(4%) died.52(71%) were with ST-T depression and 21(29%) with normal ECG.Coronary angiography revealed 54(74%) with lesions in more than one vessel and 19(26%) in one vessel (P

People departure from the treatment, prevention and health care purposes, intermittently or continuously excessive use of drugs with dependence, will cause serious mental and physical damage, while serious social harm.Opioids are the most commonly abused drug, abusers by taking an excess number of pills orally or by crushing the pills, followed by smoking, snorting, or injecting the new altered formulation to get euphoric.The escalating abuse of opioids has recently spawned the development of novel drug formulations resistant to various methods of tampering and misuse.This article discusses available opioids that include abuse-deterrent mechanisms as well as such agents currently in development.

Objective To investigate the proper dose of propofol injected intraperitoneally ( i.p.) in neonatal rats and to study the regional distribution of propofol at different doses in the neonatal brain.Methods Part I:Sixty postnatal 7-day-old rats were randomly divided into 5 groups, which received different doses of propofol injected i.p.The behavior, anesthetic intervals and arterial blood gas were recorded.Part II: Twenty neonatal rats were randomly divided into 2 groups:anesthesia group ( group A) and sedation group ( group S) , and were injected propofol i.p.at the proper dose ac-cording to the results of Part I.Rats were decapitated when they reached the ideal anesthesia depth.The regional concen-tration of propofol in different regions of the brain was examined by high performance liquid chromatography.Results 25 mg/kg propofol i.p.was the sedate dose for neonatal rats, while 75 mg/kg i.p.was the anesthetic dose.In the group S, the concentration of propofol in the thalamus was significantly higher than in other regions (P<0.05), while in the group A, the concentrations of propofol in the frontal and parietal cortex were obviously lower, and the concentrations of propofol in the hippocampus and cingulate gyrus were obviously higher than that in other regions (P<0.05).Conclusions Propo-fol is a suitable anesthetic for neonatal rats and its distribution in the brain is quite different when given at different doses.

Objective To determine the effect of age,body figure and risk framing in making treatment decision for female patients with breast cancer.Methods Two groups of healthy female:one group of 60 female ages 18-24 from undergraduate student and the other group of 60 female ages 35-60 from the university community.Healthy women imagined that they had been diagnosed with breast cancer and received information regarding lumpectomy versus mastectomy and recurrence rates.Participants indicated whether they would choose lumpectomy or mastectomy and reasons.Results The different concern levels of body appearance between younger and older women influences treatment selection of lumpectomy versus mastectomy.Facing the risk information,older women were unlikely to select the breast conserving operation.Conclusions The factors of age and the attention to body figure would effect the choice of breast conserving operation or mastectomy.Risk information on the selection of treatment would not affected directly,but the effects of risk information on younger and older female is different.Nurses should provide the age-appropriate information who recently have been diagnosed with breast cancer regarding treatment alternatives to ensure their active participation in the decision-making process,female who have different levels of investment in body figure also may have different concerns about treatment,and should be alert on empathetic of such concerns.