Objective To explore the designing and delivery strategies of nursing interventions, in order to improve the design level and delivery effect. Methods We expounded the idea through litera-ture review and the author′s practical experience in research work. Results The adoption and design of nursing intervention were discussed from literature review, theoretical framework, meta- analysis, and expert opinions. Strategies were recommended for effective delivery of nursing intervention, including integrity of in-tervention, compliance of participants, and randomization of grouping. Conclusions The above strategies should be insisted to design and carry out nursing intervention research, in order to make the intervention applicable, standardized and easy to generalize in clinic.

Objective To explore the quality of life and its influencing factors among permanent colostomy patients. Methods Totally 219 permanent colostomy patients were recruited by convenient sampling method from January 2013 to December 2014 and investigated by Stoma Quality of life-Chinese Version, Stoma self-efficacy Scale, Stoma Self-care Scale-general version , and demographic questionnaire. Data were analyzed by SPSS 17.0. Results The score of Stoma-QoL-C among permanent colostomy patients was (54.86±12.17) points, which showed a dynamic V-type change as time went by. The quality of life of patients with colostomy for 1 to 3 years was especially low (50.46±13.77) points, P<0.01. The influencing factors of quality of life among permanent colostomy patients included self-efficacy, body image change, family members′acceptance of stoma except their spouses and self-care ability, while self-efficacy was the most important influencing factor. Conclusions The enterostomal nurses should pay more attention to the quality of life of the patients who have had colostomy for 1 to 3 years, enhance the patients self-efficacy and self-care ability, and help the patients to accept their body image changes and their family members′acceptance of stoma to improve their quality of life.

Objective To investigate the effects of the HLA-Ⅰ and co-stimulators CD_(80)and CD_(86)on HT-29 cells surface, after heat killed Bifidobacterium and HT-29 cells were mingled culturing for 12 hours. Methods The HLA-Ⅰ and co-stimulators CD_(80)and CD_(86)on HT-29 cells surface were measured by Flow Cytometer. Results Heat killed Bifidobacterium can improve the expressions of HLA-1, CD_(80)and CD_(86)on HT-29 cells significantly, the livability of colon carcinoma cell was more than 90 %. Conclusion Heat killed Bifidobacterium have potential antitumorous features.

Cardiovascular System ; metabolism ; Humans ; Lung ; metabolism ; Nanoparticles ; metabolism

Nanotubes, Carbon ; adverse effects ; chemistry

Objective To analyse the transcriptome sequencing results of Eucommia ulmoides calli under light and dark culture condition, and verify the expression level of the related genes of flavonoid biosynthesis from transcriptome sequencing results. Methods The transcriptome high-throughput sequencing of E. ulmoides calli was performed by using the Illumina HiSeqTM 2500 sequencing platform, and de novo assembly of the transcriptome sequencing results was finished by Trinity software. The sequencing results of Unigenes were compared with the databases of NR, Swiss-Prot, GO, COG, KOG, KEGG and Pfam by BLAST software. The gene expression abundance was estimated by FPKM value. qRT-PCR was used to verify the expression level of the related genes of flavonoid biosynthesis. Results About 13.00 Giga base pairs (Gbp) clean data (more than 6.02 Gbp, respectively) were obtained, and de novo assembly generated 62 030 Unigenes with an average length of 736.40 bp. A total of 25 167 Unigenes were annotated to Nr. Totally 4 794 Unigenes and their associated enzymes (enzyme commission numbers) were annotated to 82 KEGG pathway. There were 1 986 genes identified as significantly and differentially expressed genes between the two calli under light and dark culture. Among them, 1 139 (57.35%) were up-regulated and 847 (42.65%) were down-regulated in the calli under light culture. Metabolic pathway analysis revealed that seven Unigenes were predicted to be responsible for the flavonoid biosynthesis, six Unigenes of which were up-regulated in the calli under light culture, encoding chalcone isomerase (EC 5.5.1.6), chalcome synthase (EC 2.3.1.74), flavonoid 3’-monooxygenase (EC1.14.13.21), trans-cinnamate 4-monooxygenase (EC 1.14.13.11), Flavonol synthase (EC 1.14.11.23), shikimate-O-hydroxycinnamoyl transferase (EC 2.3.1.133). One Unigene was down-regulated in the calli under light culture, which encoded leucocyanidin oxygenase (EC 1.14.11.19). qRT-PCR analysis showed that the expression of the seven genes related with flavonoid biosynthesis was coincident with the transcriptome high-throughput sequencing results. Conclusion The white light (12 000 lx, 16 h light and 8 h dark) could improve the production capacity of some metabolic intermediate of flavonoid biosynthesis pathway of E. ulmoides calli.

Adipocytes ; pathology ; Angiomyoma ; pathology ; Humans ; Nasal Cavity ; Nose Neoplasms ; pathology

Objective To investigate the effects of the folic acid on human T lymphoid leukemia cell line CEM cells. Methods 1. MTT method was used to detect the proliferation of CEM cells co- cultured with folic acid of different concentrations and time;2. E-xamine the changes of morphology by light microscopy with Giemsa stain;3. Detect the percentage of apoptosis and cell cycle distribution as well as the expression of the apoptosis protein(Bcl- 2,C- myc) by flow cytometry;4. Detect DNA fragments by Agaiose elec-trophoresis;5. Detect the influence of folic acid to the anticancer effects of methotrexatc(MTX) by MTT methods. Results 1 Folic acid could inhibit the proliferation of CEM cells, and the optimal inhibitive concentrations range from 0. 4 ? 10-4 ?g/L to 3. 0 ? 10 -4 ?g/L,the inhibition rate was about 30% - 40% ; 2. Co - cultured with folic acid at 24,48, 72 hours, examined by light microscopy with Giemsa stain, apoptosis cells were found in all study groups but the higher apoptosis rate was found co - cultured with folic acid at concentration of (0.4 - 3.0) ? 10-4?g/L;3.The highest apoptosis rate was 6. 19% found at the concentration of 3 ? 10-4 ?g/L, but the cell cycle distribution had no statistical difference with control group, the expression of apoptosis related protein Bcl - 2 and C-myc was decreased;4 DNA was extracted from CEM cells co - cultured with 0.4? 10 -4 ?g/L and 3 ? 10-4 ?g/L folic acid for 48 hours. UNA ladders were visible by agarose electrophoresis of DNA fragments; 5. Folic acid did not affect the antitumor effect of MTX at the concentration from 0 2? 10-4 ?g/L to 12.0?10-4 ?g/L. Conclusion Folic acid may suppress proliferation and induce apoptosis of CEM cell

Objective To study the effect of shenfu injection on the neuron apoptosis in hippocampal CA1 region of newborn with hypoxic-ischemic brain damage(HIBD).Methods The experiment included 2 parts.One was to measure the apoptosis rate by the flow cytometry,and the other was to investigate the expression of Bcl-2 and Bax of neurons in left hippocampal CA1 region.Models of postnatal 7-day Sprague-Dawley(SD)rats with HIBD were established,and were equally divided into 4 groups:sham operation(group S),control group(group C),shenfu injection pretreatment(group P),and shenfu injection treatment(group SF).The neuron apoptosis rate in hippocampal CA1 region in every group was measured at 2 h before and 2,12,24 h,3,7,14 and 28 d after hypoxic ischemic(HI) insult.The expressions of Bcl-2 and Bax were performed by immunohisochemistry.Results The apoptosis of neuron in hippocampal CA1 region in group P and group SF after HI insult was significantly less than that of group C(P

Dust ; analysis ; prevention & control ; Inhalation Exposure ; prevention & control ; Nanoparticles ; Occupational Exposure ; prevention & control ; Workplace