Adenoma ; diagnosis ; therapy ; Humans ; Male ; Middle Aged ; Parathyroid Neoplasms ; diagnosis ; therapy

Humans ; Male ; Middle Aged ; Parathyroid Neoplasms

0.05),and the distribution of genotypes was in Hardy-Weinberg equilibrium.Data analysis showed Met326Ile variant did not impact on FIns,FPG,HOMA index,BMI and gene expression of PI3-K,though the expression of PI3-K gene was lower in type 2 diabetes than in control group.Conclusion The Met326Ile variant of the p85? regulatory subunit of PI3-K is likely to be functionally normal in type 2 diabetes and health people.

A set of specific primers was synthesized according to DNA sequence of HBV found in China, the reverse transcriptase (RT) region in polymerase gene was amplified by PCR method from the serum of 3 patients with chronic HBV infection, and then the PCR products were subcloned into pGEM Teasy vectors. 13 clones were sequenced. Sequence comparison of the selected clones was made to look for the difference. After being compared, 13 sequences of RT were found different. Besides 2 clones with long sequence deletion, the different rate of RT coding nucleic acid sequences, RT and HBsAg amino acid sequences of the 11 clones is 5 1%, 4 9% and 7 5%, respectively. Many mutation types, including point substitution and deletion mutation, were found in this region. There is quasispecies population and defective HBV genome in patients with chronic HBV infection.

Objective To construct plasmid pVR1012 M as nuclei acid vaccine for hepatitis B,was constructed to immunize mice with or without plasmid pcDNA 3.1 - IL 18 to identify the effect of Interleukin 18(IL 18). Methods Polymerase chain reaction method was used to amplify the PreS2 and S region of HBV and reconstruct plasmid pVR1012 M as nuclei acid vaccine. Plasmid pcDNA 3.1 - IL 18 was used as a co stimulator. Twenty five Balb/c mice were divided into 3 groups, group 1 immunized with 100 ?g plasmid pVR1012 group 2 pVR1012 M,Group 3,pVR1012 M with pcDNA 3.1 - IL 18, respectively, every 2 weeks for 3 times. Anti HBs were detected in serum 2 weeks after each injection. Lactated ehydrogenase (LDH) cytotoxicity assay was done to analyze the cytotoxic T lymphocytes funciton. Results The positive rate and the antibody titer of serum from mice injected pVR1012 M increasing gradually with the increasing frequency of inoculation, while those from mice injected pVR1012 M and pcDNA 3.1 - IL 18(joint group) were lower than those injected with pVR1012 M alone (Difference after 3rd inoculation was significant, P

Objective T7 cDNA phage display system and bioinformatics methods were employed to find the binding protein to the PreS1 protein of hepatitis B virus (HBV). Methods PreS1 protein was coated in ELISA plate as the target protein, and then T7 cDNA library phage display system was used to scan the binding protein or peptide. A piece of cDNA was found to have the function to bind the PreS1 protein, and the product was named as PreS1 binding protein (PreS1BP). Using BLAST in GenBank, the amino acid sequence of PreS1BP was compared in the protein sequence database. Results The amino acid sequence of PreS1BP was identified as a piece of glioma tumor suppressor candidate region gene 2 (GLTSCR2), and the length of cDNA of PreS1BP was proved to be 1436 nt. The gene was located at chromosome 19q arm (19q13.3) with a length of 11445 base pair between 10403483 and 10414989, containing 13 exons and 12 introns. Conclusion HBV PreS1BP gene could be obtained by T7 cDNA phage display system in combination with bioinformatics methods.

Objective To investigate the micrometastases of No 13 lymph node in gastric carcinoma. Methods In this study, the expression of CK20mRNA, a specific tumor marker of gastric carcinoma, was detected by RQ-PCR for No. 13 group lymph node micrometastasis in 44 gastric carcinoma patients undergoing D2 radical gastrectomy plus No 13 lymph node dissection. Patient's survival was compared with those of 49 gastric cancer eases receiving standard D2 gastrectomy. Results No 13 lymph node micmmetastasis was detected in 11 out of the 44 gastric cancer cases (25%). Micrometastasis was correlated with tumor histological type (P < 0.01) such as mucoid adenocarcinoma and ring cell carcinoma, but not with age, sex, tumor location, tumor size, Bonnann type, depth of invasion (P > 0.05). Group No13 lymph node micrometastasis was detected in 2 out of 6 cases with positive No[2 lymph nodes and in 4 out of 11 cases with positive No14 lymph nodes, the differences were significant with P <0.01, and P < 0.01 respectively, when compared with those with negative No12 lymph nodes and No14 lymph nodes. After a respective median 448 and 419 days follow-up, no obstructive jaundice caused by No 13 lymph node metastasis was detected after No 13 lymph node dissection in radical gastrectomy for gastric carcinoma and such obstructive jaundice was found in one case in the control group. However, there was no significant difference in tumor recurrence between the two groups (Log Rank x2 = 0.426, P = 0.514). Conclusions Dissection of No 13 lymph node in D2 gastrectomy for gastric carcinoma is recommended in patients with poor-differentiation adenocarcinoma, mucous-cell cancer and signet-ring cell cancer, or when No 12 and No 14 lymph node metastasis is suspected.

0.05).CD34 showed widespread expression in cholangio-carcinoma tissues,but limited in normal bile duct,which showed significant difference(P

0.05). CONCLUSION: The scent of camphor and perfume bas negative influence on the learning and memory ability in mice. This may be induced by reducing the phosphorylation of CREB protein. While the scent of apple has no effect on learning and memory ability and hippocampal CREB and pCREB expression.

Pancreatic cancer is the third leading cause of cancer-related death in the world due to its high malignancy, difficult in early diagnosis, poor treatment efficacy, and high mortality. In recent years, benefiting from the progress of combined chemotherapy and neoadjuvant therapy, the prognosis of patients with pancreatic cancer has been improved to a certain extent. However, compared with other tumors, progress of precision medicine in pancreatic cancer is slow. The authors introduce the latest progress and difficulties in precision medicine of pancreatic cancer including molecular classification based on single gene and omics, monitoring tumor progression and guide treatment by minimally invasive liquid biopsy, as well as targeting therapy and immuno-therapy, in order to accelerate the development of precision medicine of pancreatic cancer.