1.Drug-resistance of Pathogens in Cardiovascular Hospital
Chinese Journal of Nosocomiology 2006;0(02):-
OBJECTIVE To investigate the pathogens and their drug-resistance in Fuwai Cardiovascular Hospital and provide antibiotics use suggestion for clinical treatment.METHODS The pathogens were identified by VITED 32 and analyzed by WHONET 5.4 RESULTS The pathogens mainly consisted of Gram-negative bacilli,which were highly sensitive to imipenem and meropenem except Pseudomonas aeruginosa;E.faecalis was much more sensitive to penicillin and gentamicin than E.faecium.Most coagulase-negative Staphylococcus(CNS) were resistant to oxacillin and showed low susceptibility rates to most antibiotics.No Gram-positive cooci were found to be resistant to vancomycin and teicoplanin.CONCLUSIONS To investigate the pathogens and their drug resistance is very important to prevent and control nosocomical infections.
2.Antibiotics Resistance in Surgery Wards of Fuwai Cardiovascular Hospital
Chinese Journal of Nosocomiology 1994;0(01):-
OBJECTIVE To investigate the major pathogens and their antibiotics resistance in surgery wards in Fuwai Cardiovascular Hospital.METHODS The pathogens were classified and their antibiotics resistance was analyzed.RESULTS The pathogens mainly consisted of Gram-negative bacilli,which were sensitive to cefoperazone/sulbactam,pipercillin/tazobactam and amikacin with the sensitive rate of 62.7-97.6%.All Gram negative bacilli were sensitive to imipenem except Pseudomonas aeruginosa,with the sensitive rate of 95.2%~100%.In the Gram-positive bacteria,the susceptibility rates of Enterococcus faecium were lower than E.faecalis.Coagulase negative Staphylococcus and Staphylococcus aureus were mainly resistance to oxacillin and most other antibiotics,all Gram positive bacteria were sensitive to vancomycin.CONCLUSIONS To know the major pathogens and their susceptibility rates can prevent and control nosocomial infections effectively.
3.Extraction Technique of Total Flavonoids from Ephedrae Radix Et Rhizoma
Dongyan CHENG ; Lishu WANG ; Jun GAO ; Donghong CHENG ; Chaonan WANG
China Pharmacist 2016;19(3):427-429
Objective:To optimize the extraction process of the total flavonoids from Ephedrae Radix Et Rhizoma. Methods:The purification method of the total flavonoids from Ephedrae Radix Et Rhizoma was optimized with the yield and content of the total fla-vonoids as the indices. Based on the above research, the process parameters were optimized by an orthogonal test. Results:The opti-mum purification conditions were as follows:the volume fraction of ethanol was 50%, the stirring extraction time was 20 min, and the liquid-solid ratio was 8∶ 1(ml·g-1). Conclusion:The optimum purification technology is simple and reproducible, and suitable for the industrial production.
4.Determination of Mahuannin A in Ephedrae Radix et Rhizoma by HPLC-ELSD
Dongyan CHENG ; Lishu WANG ; Jun GAO ; Donghong CHENG ; Chaonan WANG
China Pharmacist 2016;19(2):360-361
Objective:To establish an HPLC-ELSD method for the determination of mahuannin A in ephedrae radix et rhizoma. Methods:The content of mahuannin A was determined by an HPLC-ELSD method on an Alltima TM C18 column (250 mm × 4. 6 mm, 5 μm). The mobile phase was acetonitrile-water (28∶ 72) with a flow rate of 0. 7 ml·min-1, and the column temperature was 30℃. The temperature of drift tube heater was 105℃ and the flow rate of carrier gas was 2. 8 L·min-1 . Results:The linear range of mahua-nnin A was 42. 56-383. 04 μg·ml-1(r=0. 999 8). The average recovery and RSD was 99. 9% and 1. 96%(n=6), respectively. Conclusion:The method is simple and the result is accurate. It can be used for the quality control of ephedrae radix et rhizaoma.
5.HPLC fingerprint of Radix Salviae Miltiorrhizae from Xiangdan Injection
Yixiang WANG ; Jun ZHENG ; Zhongliang CHENG ; Xiangyan CHENG
Chinese Traditional Patent Medicine 1992;0(08):-
Objective:To study the fingerprint of Radix Salviae Miltiorrhizae of Xiangdan Injection. Methods : HPLC with UV detector was used to analyze the patterns of the Radix Salviae Miltiorrhizae of Xiangdan Injection. Protocatechuic aldehyde was used as internal standard substance. Results : The fingerprint of Radix Salviae Miltiorrhizae of Xiangdan Injection was set up and the fingerprint of them showed an excellent correlation. Conclusion : The study is helpful for the quality control of Xiangdan Injection.
6.Changes in energy metabolism and serum enzyme biomarker under static load in rabbits.
Can WANG ; Su ZHAO ; Cheng-Jun YANG
Chinese Journal of Applied Physiology 2008;24(2):150-165
Animals
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Creatine Kinase
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blood
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Energy Metabolism
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Lactate Dehydrogenases
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blood
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Male
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Pressure
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adverse effects
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Rabbits
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Serum
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enzymology
8.STUDY ON THE HISTOCHEMISTRY OF ENZYMES FROM CHORIONIC VILLI IN EARLY PREGNANCY AND ITS POSSIBLE USE IN PRENATAL DIAGNOSIS
Cheng-Lin LI ; Jian-Jun WANG ;
Acta Anatomica Sinica 1989;0(S1):-
To try to find out the possible use of histochemistry in the prenatal diagnosis in first trimester of pregnancy, 20 kinds of enzymes in chorionic villi from 6th to 9th week of gestation which are related to inborn errors of metabolism were investigated and graded according to their histochemical reactive intensity; the enzymes listed below: (1). ?-glucuronidase, (2). ?-galactosidase, (3). arylsulfatase, (4). ?-N-acetylglucosaminidase, (5). 3?-hydroxysteroid dehydrogenase, (6). NADH dehydrogenase, (7). adenosine triphosphatase, (8). alkaline phosphatase,(9). acid phosphatase, (10). creatine phosphokinase, (11). glutamic dehydrogenase, (12). aldolase, (13). xylitol dehydrogenase, (14). alcohol dehydrogenase, (15). xanthine oxidase, (16). catalase, (17). ornithine carbamoyl transferase, (18). lipase, (19). cholinesterase, (20). ?-glutamyl transpeptidase. The experimental results show that the last 6 kinds of enzymes present negative reactions. Since the his- tochemical reactions of the first 14 kinds of enzymes are positive, it is possible that they can be used for the prenatal diagnosis of inborn errors of metabolism caused by dificiency of corresponding enzymes. The results also show that compared with biochemical method, histochemical method has the advantages of requiring smaller amount of chorionic villi, being without contamination by maternal cells, simplicity and rapidity.
9.EFFECT OF TOTAL ARALOSIDES ON PROSTAGLANDINS AND CYCLIC NUCLEOTIDE
Jun REN ; Jinxuan CHENG ; Zhengang WANG ;
Chinese Pharmacological Bulletin 1987;0(03):-
The total aralosides extracted from Aralia elata (Miq) Seem. were studied in mice ( 50 mg/kg/d for 7 d ) to investigate the pharmacological mechanisms on the molecular level.The results showed that aralosides can obviously stimulate the production of PGE2、PGF2, and cAMP but reduce the cGMP level and nave no effect on histamine release.It is suggested that the changes in the level of cyclic nucleotide might be due to the alternations of PGs, which is now been suspected as the "Second Messenger" . These findings could explain in part the pharmacological activities of aralosides on the molecular level.
10.The clinical study of twice chemonucleolysis with collagenase for the treatment of lumbar disc herniation
Zhiqun WU ; Zhimin WANG ; Jun CHENG
Journal of Interventional Radiology 1994;0(04):-
Objective To study the feasibility and indication of twice chemonucleolysis with collagenase for the treatment of lumbar disc herniation.Methods Eighty two patients of lumber disc herniation were treated with twice collagenase chemonucleolysis.All patients were followed up for 3 to 12 months and then the clinical results were assessed according to the Macnab criteria retrospectively.Results Eighty two cases were followed up from 3 to 12 months postoperatively.Fifty one cases were excellent,13 cases good,8 as fair and 10 were poor.The rate of excellent plus good reached 78%,the effective rate was 88%.Conclusion Twice chemonucleolysis with strict indications together with prevention of allergic reaction before,during and after the operation;is safe and effective for treating lumbar disc herniation.(J Intervent Radiol,2007,16:258-259)