1.Protective effect of antioxidative ?-lipoic acid on kidneys in type 2 diabetic rats
Bo FENG ; Xin-Feng YAN ; Lei XU ; Qiao-Hui QIAN ; Hua WANG ; Jun-Li XUE ;
Chinese Journal of Endocrinology and Metabolism 2001;0(05):-
There were significant increase in urine protein excretion,raised malondialdehyde(MDA) level and expressions of NF-?B,p22phox and p47phox in renal tissue,and significant decrease in reduced glutathione,superoxide dismutase,vitamin C and E levels in type 2 diabetic Goto-Kakisaki rats after 12 weeks. There was obvious histomorphologic change in the kidneys.All the above indices were improved by intraperitoneal injection of?-lipoic acid(35 mg/kg q.o.d).Besides,significant positive correlations were found of MDA level to p22phox,p47phox and NF-?B in the renal tissue,?-lipoic acid seems to protect the diabetic kidney in this diabetic rat model via antioxidative effects.
2.Study on the health standard for phosphorus pentasulfide in the workshop air.
Chun-Mi LAI ; Shu-Bo LIU ; Shun TAO ; Jian-Yun DAI ; Yun GAO ; Wei-Jun LI ; Shu-Qiao CAO
Chinese Journal of Industrial Hygiene and Occupational Diseases 2004;22(4):310-311
Adult
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Air Pollutants, Occupational
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adverse effects
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Chemical Industry
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Female
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Humans
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Insecticides
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adverse effects
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Male
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Maximum Allowable Concentration
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Middle Aged
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Occupational Diseases
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chemically induced
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diagnosis
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Organothiophosphorus Compounds
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adverse effects
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Phosphorus Compounds
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adverse effects
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Sulfides
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adverse effects
3.Analysis and Treatment of Multiple Severe Venous Vascular Malformation Syndrome Combined with Coagulopathy.
Jun-Bo QIAO ; Jin LI ; Xie-Fu ZHANG
Chinese Medical Journal 2015;128(18):2546-2548
Adolescent
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Adult
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Anticoagulants
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therapeutic use
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Blood Coagulation Disorders
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diagnosis
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drug therapy
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Female
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Humans
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Male
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Middle Aged
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Vascular Malformations
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diagnosis
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drug therapy
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Veins
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pathology
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Young Adult
4.Dynamic Changes of Phenotypes and Secretory Functions during the Differentiation of Pre-DCs to Mature DCs
WANG JING ; ZHAO LI-BO ; CHANG SHENG ; MING CHANG-SHENG ; YANG JUN ; GONG NIAN-QIAO
Journal of Huazhong University of Science and Technology (Medical Sciences) 2017;37(2):191-196
The dynamic expression of cytokines and phenotypes during the differentiation process of dendritic cell precursors (pre-DCs) to mature dendritic cells (DCs) was investigated,and the effects of inflammatory stimulation with lipopolysaccharide (LPS) on DCs differentiation were understood.The differentiation of bone marrow cells isolated from Balb/c mice was induced to DCs in an 8-day cell culture system with RPMI-1640 complete culture medium containing 10% FBS,20 ng/mL recombinant mouse granulocyte-macrophage colony-stimulating factor (rmGM-CSF) and 10 ng/mL recombinant mouse interleukin-4 (rmIL-4).On the day 3,6 and 7 after culture,DCs were divided into non-LPS group and LPS group,given 500 ng/mL LPS for 24 h stimulation and no stimulation respectively.The expression levels of CD 1 l c+,MHC-Ⅱ +,CD40+,CD80+ and CD86+ were detected by flow cytometry,and those of IL-2,IL-4,IL-10,IL-12 p70 and IFN-γ in the supernatant by ELISA.On the day 3 and 6 after culture,the expression of IL-2,IL-4,IL-10 and IFN-γ in DCs showed no significant differences between non-LPS group and LPS group,whereas the differences were significant at day 7.The expression levels of cytokines (for IL-2,IL-4,IL-10,IFN-γ and IL-12 p70:152.86±6.91,778.33±8.35,44.55±2.54,5.8.26±1.09 and 2423.00±57.21 pg/mL respectively) in LPS group were higher than those in non-LPS group,especially IL-12 p70 increased obviously at day 7.It was concluded that during the differentiation process of pre-DCs to mature DCs,LPS stimulates DCs producing large amounts of IL-12 p70 and Thl-type cytokines as compared with Th2-type cytokines,and day 7 may be a key time-point for DCs polarization.
5.Research on antitumor effects of small molecule inhibitors of deubiquitinases: new progress and new ideas
Xiang-ning LIU ; Jia-min DU ; Mei-jia QIAN ; Xiao-wu DONG ; Bo YANG ; Hong ZHU ; Qiao-jun HE
Acta Pharmaceutica Sinica 2022;57(3):547-556
The abnormality of ubiquitin proteasome pathway is an important factor leading to the imbalance of protein homeostasis. In this process, the deubiquitinase responsible for removing the ubiquitin chain of protein substrate is very important. Its abnormal activity or expression can cause the functional changes of key oncogenic/tumor suppressor proteins, which directly or indirectly lead to the occurrence, development and malignant evolution of tumors. Based on this, the discovery and research of small molecule inhibitors targeting deubiquitinases have become a hot field of anti-tumor candidate drugs. This review will focus on the regulatory effect and mechanism of ubiquitin proteasome pathway, especially deubiquitinase on tumor, introduce the application of deubiquitinase small molecule inhibitors in tumor treatment, and discuss the research status and latest progress of small molecule inhibitors, so as to provide ideas for the research of new anti-tumor strategies based on deubiquitinase.
6.Recent advances in mechanisms of KRASG12C inhibitors anti-tumor resistance and relevant overcoming strategies
Ke-xin LIU ; Rui-lin WU ; Tao YUAN ; Kai-yue PU ; Qiao-jun HE ; Hong ZHU ; Bo YANG
Acta Pharmaceutica Sinica 2022;57(2):271-276
KRAS is one of the most frequently mutated human oncogenes. In spite of mounting efforts on the development of direct or indirect inhibition targeting KRAS, little has been achieved because of insurmountable difficulties, titling KRAS "undruggable". Recently, subtype-specific inhibitors have shown great hope. Some KRASG12C inhibitors have entered clinical trials, including adagrasib and sotorasib, and have shown preliminary clinical effectiveness. Experiences from the inhibitors targeting the downstream factors of RAS pathways show that the anticancer activity of these drugs will be limited due to the development of drug resistance. Preclinical studies of KRASG12C inhibitors have revealed that the application of these agents might be hampered by the drug resistance issue. The current review aims to describe the current status of KRASG12C inhibitors, and discuss the mechanisms underlying KRASG12C inhibitor resistance, so as to provide the clues for the combat of drug resistance.
8.A new surgical treatment for thumb (finger) reconstruction by the free moulded second toe transfer
Jing-Liang ZHANG ; Zhen-Rong XIE ; Jun-Bo XIAO ; Yan-Wen LEI ; Jun SONG ; Qiao-Hong GUO ; Hang LI ; Zhong-Ming HUANG ; Huan-Wei CHEN ;
Chinese Journal of Microsurgery 2006;0(05):-
Objective To investigate a more perfect method for a nice outward appearance of the thumb(finger) reconstructed.Methods An artery pedicle composite flap from fibular side of the great toe is inlaid in the tibial(ventral) side of the free second toe for thumb reconstruction and the same free second toe with a distal part of metatarsal bone with a double-wings flap for finger reconstruction before transplantation. Results The reconstructed thumb(finger) gets a nice looking and normal function while no blight to the great toe occurred.Conclusion It is an effective new procedure in ameliorating outward appearance of the recon- structed thumb(finger) by transferring the free moulded second toe.
9.Development of a print quality inspection system for biochips.
Ai-Ke QIAO ; Xian-Long MENG ; Zhang-Jun MA ; Hong-Bin ZHANG ; Bo CHU
Chinese Journal of Medical Instrumentation 2008;32(6):434-437
An automatic inspection system for biochip's print quality is presented in this paper. It consists of an automatic mechanical control, a CCD sensor for getting the image of PET boart, and the special computer software for image processing and recognition. Experimental results indicate that this system is capable of providing a precise and effective realtime inspection for biochips' print quality.
Biosensing Techniques
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instrumentation
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methods
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Equipment Design
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Image Processing, Computer-Assisted
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methods
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Microchip Analytical Procedures
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methods
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Pattern Recognition, Automated
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methods
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Quality Control
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Software
10.Effects of Rho/ROCK signal pathway on AGEs-induced morphological and functional changes in human dermal microvascular endothelial cells..
Ji-Ping WANG ; Xiao-Hua GUO ; Ling-Jun WANG ; Qiang LI ; Bo CHEN ; Wei WU ; Xu-Liang HUANG ; Qiao-Bing HUANG
Acta Physiologica Sinica 2009;61(2):132-138
The present study aimed to determine the role of Rho/Rho kinase (Rho/ROCK) phosphorylation on advanced glycation end products (AGEs)-induced morphological and functional changes in human dermal microvascular endothelial cells (HMVECs). HMVECs were respectively incubated with different concentrations of AGEs-modified human serum albumin (AGEs-HSA) for different time. In some other cases, HMVECs were pretreated with ROCK inhibitors (H-1152 or Y-27632). The morphological changes of F-actin cytoskeleton were visualized by rhodamine-phalloidin staining and the phosphorylation of Rho and ROCK were determined by Western blot. Endothelial monolayer permeability was assessed by measuring the flux of FITC-albumin across the endothelial cells. The results showed that the distribution of F-actin was significantly altered by AGEs-HSA in time and dose-dependent patterns. These effects were inhibited by ROCK inhibitors. The phosphorylation of Rho and RCOK was remarkably increased by AGEs-HSA treatment while total Rho and ROCK protein levels were not affected. The permeability of endothelial monolayer was dramatically increased by AGEs-HSA, and both ROCK inhibitors (H-1152 or Y-27632) attenuated these hyperpermeability responses. The results obtained suggest that the phosphorylation of Rho/ROCK plays an important role in AGEs-induced morphological and functional alterations in HMVECs.
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
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analogs & derivatives
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pharmacology
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Actin Cytoskeleton
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metabolism
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Actins
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metabolism
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Amides
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pharmacology
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Endothelial Cells
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metabolism
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Endothelium, Vascular
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cytology
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Fluorescein-5-isothiocyanate
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analogs & derivatives
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metabolism
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Glycation End Products, Advanced
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pharmacology
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Humans
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Phalloidine
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analogs & derivatives
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Phosphorylation
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Pyridines
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pharmacology
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Rhodamines
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Serum Albumin
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metabolism
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pharmacology
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Serum Albumin, Human
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Signal Transduction
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rho-Associated Kinases
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metabolism