OBJECTIVE: To observe the effect of the polyglycol (PEG) modification of trimeric ? peptide(?3) on it’s anti-metastasis ability. METHODS: Using adhesion test to study the effects of ?3 and ?3-PEG on the adhesion of tumor cells to FN. Using artificial basal membrane to study the effects of ?3 and ?3-PEG on the invasion and recombination of basal membrane of tumor cells. RESULTS: Comparing to negative control,?3 and ?3-PEG could both inhibit the adhesion of SMMC-7721 and HCCLM6 tumor cells to FN with time-dependently(P

Objective To investigate the protective effect of total flavonoids of drynaria on hemorrhagic shock reperfusion renal injury.Methods The 30 Sprague-Dawley rats were randomly divided into 3 groups: sham operation group, experimental group (hemorrhagic shock group) and drynaria fortunei group, 10 in each group. In drynaria fortunei group and experiment group, the blood from the femoral artery was with drawn until the mean arterial pressure reached 40 mmHg in 90 min, and then the withdrawn blood transfusion were reinfused and blood pressure recovered to the base value of 90%. The rats of drynaria fortunei group were given intraperitoneal injection of Drynaria total flavonoid 300 mg/kg, and experimental group and sham operation group were injected with 3 ml physiological saline. Blood creatinine (SCr) levels, the renal cell apoptosis index, the expression of Bcl-2 and mRNA Bax in kidney were detected. Results Compared with the experimental group, serum SCr (78.36 ± 5.31 mmol/Lvs. 151.43 ± 11.8 mmol/L) of drynaria fortunei group decreased significantly (P<0.01); the renal cell apoptosis (19.56% ± 4.37%vs. 14.31% ± 2.83%), the expression level of Osteopractic rats of Bax group mRNA (1.24 ± 0.05vs. 0.91 ± 0.07) of drynaria fortunei group decreased significantly; and the Bcl-2 mRNA (0.65 ± 0.05vs. 1.38 ± 0.07) and the expression level and Bcl-2/bax ratio (0.52 ± 0.06vs. 1.51 ± 0.08) of drynaria fortunei group increased significantly (P<0.01).Conclusions The drynaria total flavonoids on rats with hemorrhagic shock and resuscitation of renal injury has a protective effect, and its mechanism may improve the expression of Bcl-2 and mRNA, increase the ratio of bcl-2/bax, and inhibit  or block the apoptosis of renal tissue.

Objective:To investigate and analyze the rational use of anti-tumor immune enhancement drugs in our hospital. Meth-ods:The annual use data of anti-tumor immune enhancement drugs in our hospital in 2015 were collected, and then statistically ana-lyzed and evaluated combing with the medical history information. Results: There were 12 varieties and 19 specifications of adjuvant anti-tumor immune enhancement drugs in our hospital, which accounted for 15. 87% of the total sum. Totally 25 481 patients used ad-juvant anti-tumor immune enhancement drugs, which occupied 29. 84% of the total medicine afford. The adjuvant anti-tumor immune enhancement drugs showed lots of irrational use in clinics, and the main irrationity was overuse and off-label use. Conclusion: It is necessary to strengthen prescription evaluation for anti-tumor immune enhancement drugs, which needs controlled and rational use.

Objective To discuss the pe rc utaneous laser disc decompression (PLDD) therapy by adopting the Nd: YAG Laser C L-50 Device under X-ray monitor supervision. Methods A total of 378 cases were included in this study. There was 198 males and 180 fe males, the age ranged from 19 to 76 years, with the average of 43.9 years, and the course of diseases from 1 month to 16 years. 17 cases of lumbar interverteb ral disc prolapse were diagnosed between L2 and L3, 35 cases between L3 and L4, 140 cases between L4 and L5, and 116 cases between L5 and S1. 60 cases with two intervertebral disc prolapses at the same time, and 10 cases with three interve rtebral disc prolapses at the same time. A total of 458 intervertebral-dis c operations were conducted with PLDD. All the cases selected had been examined by CT or MRI, and their clinical symptoms and physical signs were in compliance with the diagnosis standards for prolapse of lumbar intervertebral disc. Results After PLDD operation, 268 cases were cured within 1 month with a cure rate of 70.90%; 259 cases were cured in 3 months with a cu re rate of 68.52%, 260 cases were cured in 6 months with a cure rate of 68 .25%. Stable results were found with curing satisfaction rate, effective rate and failure rate. Conclusion X-ray guided PLDD therapy is an ideal non-operative therapy for curing lumbar intervertebral disc prolap se with micro-trauma, little pain, quick recovery and good effect. PLDD has ac hieved encouraging effects both in its recent clinical outcome and long term eff ect as well.

Objective To analyze and compare in vitro deposition results of budesonide inhalation suspension made by 3 jet nebulizers. Methods Self-made budesonide inhalation suspension was selected as a model drug, and the in vitro deposition properties was determined by breathing simulator and the next generation pharmaceutical impactor (NGI) with Pari Turob BOY N?BOY SX and Omron NE-C28 nebulizers. Results The mass median aerodynamic diameter (MMAD) of aerosol measured with the three kinds of nebulizers ranged between 4.95 and 6.54 µm, geometric standard deviation(GSD)ranged between 1.66 and 2.10,fine particle dose (FPD) ranged between 87.64 and 101.47 µg and fine particle fraction (FPF) ranged between 37.75% and 53.88%. Delivery rates of Pari Turob BOY N and BOY SX were similar, while the delivery rate of Omron NE-C28 was low. The total delivery of each nebulizer was about 0.11 µg. Statistical analysis showed that different nebulizer had no significant difference on delivered dose, but had significant difference on delivered rate(P<0.01)and aerodynamic measurement results(P<0.05). Conclusion Different jet nebulizers for budesonide inhalation suspension have certain influence on its in vitro deposition. The suitability of the Omron NE-C28 is the best among the three kinds of nebulizers.

Objective: To observe the influence of β peptide dimmer modified by PEG on anti-metastasis activity. Methods: Cell culture plates were coated with fibronectin (FN) as extracellular matrix (ECM). The influence of β 2 and β 2-PEG peptides on the adhesion of tumor cells to FN were observed. The effects of β 2 and β 2-PEG peptides on migration and invasion ability of tumor cells in reconstituted basement membrane were measured by using Transwell Boyden and Matrigel method. Results: Compared with negative control, β 2 and β 2-PEG peptide significantly inhibited the adhesion of SMMC-7721 and HCCLM 6 tumor cells to FN in a time- and dose-dependent manner (P < 0.05). The inhibitory effects of β 2-PEG were stronger than β 2 peptides (P < 0.05). The mobility and invasion of HCCLM 6 and SMMC-7721 cells were obviously inhibited by β 2 peptide and β 2-PEG (P < 0.05). For HCCLM 6 cells, β 2 peptide and β 2-PEG inhibited cell migration were 54.6% and 56.3% and suppressed cell invasion were 36. 8% and 46.6%, respectively. For SMMC-7721 cells, the migration inhibitory rate were 43.6% and 45.7% and invasion inhibitory rate was 33.6% and 35.9% by β 2 and β 2-PEG peptide, respectively. The difference were not significant before and after PEGylation. Conclusions: The β 2 and β 2-PEG peptides specifically inhibite the adhesion of tumor cells to FN in a time- and dose-dependent manner. The anti-adhesion effects are enhanced after PEG modification. The β 2 and β 2-PEG peptides obviously suppress migration and invasion of the two kinds of tumor cells. The difference is not significant before and after PEG modification.

Rituximab in the combination of CHOP regimen has been widely used as the standard treatment of several kinds of B cell non-Hodgkin lymphoma (B-NHL),but there are still about 1/3 of the late B-NHL patients become primary and secondary resistant to the drug.Recently,many translational research progress in malignant lymphoma promoted the development of promising candidate drugs for the treatment of lymphoma.The advances in translational research field were summarized in this manuscript.

Tremendous success has emerged in chimeric antigen receptor (CAR)-T cell therapy over the past few years, especially in leukemia and lymphoma. The first CAR-T cell product might be available in America in 2017 due to the emergence of the critical results. This paper focused on the key data presented at the 58th American Society of Hematology Annual Meeting.

BACKGROUND:It is reported that vitamin C can induce bone marrow mesenchymal stem cel s differentiating into osteoblasts, and promote bone repair and regeneration. However, vitamin C solution is unstable, so a carrier is necessary. OBJECTIVE:To observe the loading and control ed-release abilities of calcium phosphate used as the carrier ofvitamin C. METHODS:Calcium phosphate particles loaded with vitamin C were fabricated using chemical precipitation method, and the final concentration of vitamin C was 0, 0.1, 2 and 4 mmol/L, respectively. The drug-loaded capacity was detected. The release of vitamin from calcium phosphate nanoparticles in the simulate body fluid and ultrasonic environment was respectively evaluated. MC3T3-E1 cel s were co-cultured with calcium phosphate nanoparticles loaded with 2 mmol/L vitamin C, or calcium phosphate nanoparticles only. The cel proliferation was detected at 1, 3, 5 and 7 days of culture, and the alkaline phasphatase activity was detected at 1, 5, 10 and 15 days of culture. RESULTS AND CONCLUSION:The drug-loaded contents of calcium phosphate nanoparticles loading 0, 0.1, 2 and 4 mmol/L vitamin C were (59.9±5.4)%, (87.2±1.2)%and (28.4±26.3)%, respectively. Under normal environment, al samples could release vitamin C persistently, but the initial release speed of the particles carrying 0.1 and 2 mmol/L vitamin C was lower than that of particles carrying 4 mmd/L vitamin. Under ultrasonic environment, 2 mmol/L vitamin C-loaded calcium phosphate particles exhibited a quick release speed firstly that reached 5-15%, fol owed by a slow release speed. When ultrasonic powers kept at 75, 105 and 150 W, the release duration of vitamin C was 220, 340 and 260 minutes, respectively. MC3T3-E1 cel proliferation did not change after co-cultured with 2 mmol/L vitamin C-loaded calcium phosphate particles but the alkaline phosphatase activity was improved. These results suggest that calcium phosphate particles can be used as the carrier of vitamin C.