Objective:To investigate the clinical effect of fastigial nucleus electrical stimulation(FNS) treating the disorders of consciousness by brain damage,and explore the possible mechanism.Methods:9 patients with the disorders of consciousness by brain damage of different pathogeneses were studied.They were treated with FNS on the basis of routine medical treatment.And Glasgow Coma Scale (GCS),electroencephalogram(EEG) and the clinical effects were observed.Results:After FNS treatment,the level of conciousness was improved.GCS were increased 5.22?1.44,which showed significant difference compared to that before treatment ( P

Objectives; To identify the roles of Notch - 1 gene for survivaland differentiation of mesenchymal stem cells ( MSCs) into neurons by way of RNA interference ( RNAi) technique to suppress expression of Notch - 1 gene. Methods; Firstly, construct mNotch - 1 short hairpin RNA (mNotch -IshRNA) and then to oberve configuration of MSCs transfected by mNotch - IshRNA; MTT was employed to evaluate the survival ratio of MSCs before transfection, 24 hours and 3 days after transfection, respectively; agents, such as BDNF, retinoid acid, were obtained to induce MSCs transfected by mNotch - IshRNA in vitro and expression of Nestin, NSE and NF200( neuron marker agent) and GFAP( neural glial cell marker agent) were determined by immunocytochemistry stain technique. Results: The cell cubic configuration was enforced and expression of notch - 1 gene vanished by transfection of mNotch -IshRNA. The survival ratio of MSCs by transfection decreased and there was significant differences between non - ransfection and transfection - controlling group after 24 hours and 3 days of transfection ( P

Objective:To explore the clinical efficacy of distal femoral anatomical plate for the treatment of mid-inferior femoral fractures in adults. Methods:Twenty-two patients received distal femoral anatomical plates,from July 2004 to March 2007,for mid-inferior femoral fractures in our department. Their conditions of bone union and functional restoration were retrospectively analyzed. Results:All the patients were followed up for 12 ～32 months,and all the fractures obtained good union in the first stage,and no loose,bending or fragmentation of the plates were detected. Function of the elbow joints reached an excellent and good rate of 86.4%,in terms of Cassebaum rating system. Conclusion:Distal femoral anatomical plate is effective to treat middle-inferior femoral fractures.

BACKGROUND:The traditional tissue processing procedures,which includes a variety of toxic chemical reagents,both for himself and for operating environment there is a lot of damage.OBJECTIVE:To improve the traditional tissue processing procedures,to establish economic processing of tissue sample disposal,and to eliminate damages to the handlers and environment bringing by related reagents.DESIGN,TIME AND SETTING:The tissue sample processing study was performed at the Department of Pathology,Third Xiangya Hospital,Central South University from February 1998 to February 2008.MATERIALS:All specimens were routine surgical specimens of the Third Xiangya Hospital,Central South University.Acetic acid,alcohol,turpentine and paraffin(at 56-58 ℃) were obtained from Chemical Plant of Hunan Normal University,China.The automatic dehydration machine ZT-12P was purchased from Hubei Xiaogan Yaguang Medical Technology Co.,Ltd.,China.METHODS:Formaldehyde and xylene were replaced by non-toxic chemical reagents.Processing was as follows,0.7 volume fraction of ethanol and glacial acetic acid for 2 hours,0.8 volume fraction of for 1 hour,0.9 volume fraction of ethanol for 1 hour,0.95 volume fraction of ethanol Ⅰ for 2 hours,0.95 volume fraction of ethanol Ⅱ for 2 hours,dehydrated alcohol Ⅰ for 1 hour,dehydrated alcohol Ⅱ for 1 hour,turpentine oil Ⅰ for 1 hour,turpentine oil Ⅱ for 1 hour,paraffin Ⅰ for 1 hour,paraffin Ⅱ for 1 hour,and paraffin Ⅲ for 1 hour.After collecting,samples were put in automatic dehydration machine.Suitable for all kinds of human samples.MAIN OUTCOME MEASURES:To compare the tissue specimens processed by new program with the traditional processing.RESULTS:Specimens treated by improved environmental protection procedures can be sliced up smoothly,and dyed uniformity,which have marked contrast of red and blue.CONCLUSION:Improved environmental protection procedures can avoid handlers exposing to toxic chemical reagents,can entirely replace traditional procedures.

0.05).Compared the second group with control group,there was more significantly difference on LH,FSH and PRL(P0.05).Conclusion It was more accurate and comprehensive to reflect the internal secretion condition through measurement of serum five kinds of sex hormone,and to provide an important indication of reasons for fe- male sterility.

Twenty male wistar rats were divided into naloxone group and control group, and their backs were dipped into boiling water, which resulted in deep Ⅱ or Ⅲ degree burn of about 20% of BSA.Naloxone or normal saline were injected into lateral ventricle and electrocardiogram (ECG) was recorded every 30 minutes.The results showed that the ECG of burned rats had not only a prolonged P-P, P-R, QT interval and QRS, but also an elevated ST segment and R wave. These changes were greatly delayed in the naloxone group (p

150 cases with carcinoma of salivary gknds were analysed. The tumors of 61 cases were located in parotid, 14 in submaxillary, 5 in sublingual and 72 in minor salivary gknds. 49 cases were pathologically diagnosed as malignant mixed tumor ,25 adenocarcino- ma, 24 adenoid cystic carcinoma,22 mucoepidermoid carcinoma,22 acinic cell carcinoma, 5 squamous cell carcinoma , 2 papillary cystadenocarcinoma, 1 undif-ferentiated carcinoma. The 3,5 and 10 year survival rates of these 150 patients were 80. 7% ,69. 4%. 52. 1% respectively. The prognosis of acinic cell carcinoma and mucoepidermoid carcinoma was much better than that of squamous cell carcinoma, adenocarcino-ma and undifferentiated carcinoma. According to the location of the tumors, the survival rates of the patients with the tumors in minor salivary glands and parotid glands were the highest and those in sublin-gual glands and submaxillary glands were the lowest . Postoperative radiotherapy and chemotherapy may increase the survival rate of the patients with carcinoma of salivary glands. The overall recurrent rate was 27. 6% ,the neck lymph node metastasis rate was 9.2%.

Anti-Anxiety Agents ; administration & dosage ; therapeutic use ; Bridged-Ring Compounds ; poisoning ; Child ; Humans ; Male ; Midazolam ; administration & dosage ; therapeutic use ; Seizures ; drug therapy ; etiology

BACKGROUND:Total knee arthroplasty needs to use the femoral prosthesis. The prosthesis wil produce a certain axial rotation on the line, and affect the biological properties of the patelofemoral joint. OBJECTIVE: To explore the effect of axial rotation of prosthesis on biological effect of the patelofemoral joint in total knee arthroplasty. METHODS:Ten fresh human cadaver knees were obtained from the Specimen Room of Second Affiliated Hospital (Henan Province Hospital of Traditional Chinese Medicine) of Henan University of Traditional Chinese Medicine. Knee joint experimental platform was established to simulate the human ankle and hip joint, providing 3 degrees of freedom of tibia and 2 degrees of freedom of femur. Femoral component rotation angle was measured. Total knee arthroplasty was conducted. The sensitive pressure-sensitive film was used to measure the transepicondylar axis rotation of 2°, 2° through the femoral epicondylar axis rotation, the femoral epicondylar axis rotation of 4°, and paralel to the femoral epicondylar axis, external rotation of the femoral epicondylar axis 4°, and the patelofemoral contact pressure and patelofemoral contact area paralel with Whiteside’s line. RESULTS AND CONCLUSION:The peak contact pressure inside and outside the joint at different axial rotation of the femoral prosthesis paraleled with femoral epicondylar axis was in a balanced state, and significant differences were detected as compared with other rotation manners (P < 0.05). No significant difference was found in patelofemoral contact area at different directions. These findings suggest that femoral prosthesis axial rotation on the line in total knee arthroplasty wil have a certain effect on biological properties of patelofemoral joint. The peak contact pressure inside and outside patelofemoral joint in the paralel state of the femoral epicondylar axis does not appear excessive low or high state on one side, but in a balanced state. It can be chosen as a reference standard of rotational alignment of the femoral prosthesis during the operation.

Objective:To explore the effects of the treatment by internal fixation operation through pedicle of vertebral arch for thoracolumbar vertebral fracture cases.Methods:48 cases sufering from thoracic or lumbar vertebral fracture accept the treatment by internal fixation operation through pedicle of vertebral arch and intervertebral body fusion.Total or partly resection of vertebral plate was performed while needed for decompression.The lasted 18～30 months or more.Results:All the injured vertebrae had get reposition of height and the Cobb angle get well without any serious complication.Follow-up shows that every patient has get solid fusion with good height and stability,and the Cobb angle stain well.Thirty-six of the 38 spinal injury patients have get Franke1 grade raise in different degree.There were 2 Franke1A grade spinal injury cases with no neuro-function amelioration,who both got late hospital admission.Conclusion:For thoracolumbar vertebral fracture,internal fixation operation through pedicle of vertebral arch and intervertebral body fusion provide reposition of the injured vertebra height and spinal stabilization,and the spinal nerves function will get improved,and the operation indication and opportunity must be assurance.