Objective To summarize clinical data of chronic neutrophilic leukemia(CNL) in China.Methods Chronic neutrophilic leukemia was searched as key words in full text database of Chinese periodical(www. cnki. net)(from 1994 to 2008). Results 136 cases of CNL were found, including male 77 cases and female 59 cases. Male: female ratio was 1.305:1. The mean age was 57.8 (8 ～87) years old. The common symptoms was fatigue,granulocytosis and upper abdominal discomfort or tumour. The splenomegaly was found in the majority of cases. The mature neutrophilic granulocyte in peripheral blood was high and cast longer time with raised segmented and band staged of neutrophils. Serum assay showed a raised level of VitB12 and uric acid. Bone marrow aspirate showed a hypercellular marrow with marked granulocytic proliferation, consisting mainly of stab forms and segmented neutrophils. The NAP score was high. Both Ph chromosome and bcr-abl fusion gene was negtive. Conclusion CNL was a scarce chronic leukemia. It should be discriminated with leukemoid reaction and chronic granulocytic leukemia. The course of CNL patients was long or short. It could transform into acute leukemia and palliative therapy was mainly applied at present.

Dental Pulp Diseases ; therapy ; Humans ; Periapical Diseases ; therapy ; Root Canal Obturation

Dermatovenereology have developed rapidly in fields including genodermatology, dermatological epidemiology, skin immunology, infectious skin diseases, clinical diagnosis, drug treatment, light therapy, and sexually transmitted diseases in recent years. Meanwhile, many skin diseases and a few venereal diseases still lack effective ways of treatment and control. More efforts should be made in both basic and clinical researches in the related fields.
Dermatology ; trends ; Forecasting ; Humans ; Venereology ; trends

Dental Pulp Diseases ; therapy ; Dental Restoration, Permanent ; Dentist-Patient Relations ; Feeding Behavior ; Humans ; Oral Hygiene ; Periodontal Diseases ; therapy ; Tooth Loss

Recent studies have demonstrated that intravenous transplantation or local injection of bone marrow cells can induce unexpected changes of their fate. The results of these experiments showed that after transplantation or injecton, some of tissue specific somatic cells such as hepatocytes, skeleton, cardiac muscle cells and brain cells expressed the donor cell-specific genes, such as Y chromosome. There are two hypotheses that can explain this phenomenon. One is bone marrow stem cell transdifferentiation and the other is spontaneous cell fusion.

Recently,considerable progress has been made in the treatment of systemic lupus erythematosus(SLE),especially in the clinical use of new immunosuppressive agents,which leads to improved remission rate and life quality.However,plasmapheresis still shows to be necessary in severe lupus with serious complications.With the development of plasmapheresis technology,the efficiency and specificity of antibody adsorption being improved significantly,it played an important role in the treatment of acute stage life-threaten lupus.Here we intend to make a review on the relative improvement of plasmapheresis technology and its clinical use.

Dental Pulp Diseases ; therapy ; Humans ; Root Canal Therapy

Dental Caries ; etiology ; prevention & control ; Humans

Composite Resins ; Dental Restoration, Permanent ; Humans

Loop-mediated isothermal amplification (LAMP) is a novel in vitro nucleic acid amplification method conducted under isothermal conditions with the advantages of high specificity, sensitivity, rapidity and easy detection. Since it was established in 2000, it has been widely applied in various fields of analytical science including the diagnosis of a variety of pathogens, identification of embryo sex, detection of genetically modified organisms and cancer gene identification. Additionally, significant progress has been made in the optimization of the LAMP method, such as accelerated reactions, simplified sample processing, the realization of multiplex amplification, and the enhanced specificity of reaction and detection methods. LAMP technology also shows much potential to be adopted as part of point-of-care testing platforms by the micromation, automation and integration with other technologies such as Lab-on-a-Chip and digital nucleic acid amplification. This review summarizes the latest advances in the LAMP technique and its applications in developing point-of-care testing platforms.
Diagnostic Techniques and Procedures ; trends ; Humans ; Nucleic Acid Amplification Techniques ; methods ; Point-of-Care Systems ; trends ; Virus Diseases ; diagnosis ; virology ; Viruses ; genetics ; isolation & purification