Objective To investigate the possible role of eukaryotic initiation factor 6(eIF6)in the development of kidney inter-stitial tissue fibrosis in a mouse model of unilateral ureteral obstruction(UUO).Methods (1)UUO model was set up in eIF6+/-and eIF6+/+ mice.(2)HE and Masson staining were used to histologically assess the interstitial deposition of collagen in mouse kidney.Immunohistochemistry and image analysis were applied to analyze the expression of α-SMA and TGF-β1 in mouse kidney as well as eIF6 in human kidney samples.Results 10 days after unilateral ureteral obstruction,more kidney interstitial fibrosis tissues were found in eIF6+/- mice than that in eIF6+/+ mice.TGF-β1 andα-SMA were expressed more intensive in tubular epithelial cells of eIF6+/- mice than that of eIF6+/+ mice in the UUO model.Expression of eIF6 is lower in the region of fibrosis than that in non-fibrotic area.Conclusion eIF6 might be involved in the development of kidney interstitial tissue fibrosis by regulating the expres-sion of TGF-β1 .

Objective To explore the effect of methylprednisolone combined with interferon in the treatment patients with relapse remitting multiple sclerosis.Methods 90 patients with relapse remitting multiple sclerosis were randomly divided into experimental group and treatment group.The experimental group during the acute stage with methylprednisolone pulse therapy,remission with interferon β (IFN-β) treatment; the control group only in the acute phase with methylprednisolone pulse therapy.Results The total effective rate of methylprednisolone pulse therapy in the acute stage was 98.9％.Experimental group during the treatment with IFN-β1α,relapse rate was 30.2％ ;patients in the control group were followed up for two years,the recurrence rate was 53.3％.Experimental group and control group was significantly different ( P ＜ 0.05 ),the experimental group was significantly lower than the control group.Conclusion For relapsing-remitting MS,using MPPT could relieve acute symptom in the acute stage,and in remission using of IFN-β1α relapse prevention was a good choice for clinicians.

Cochlear Implants ; Magnetic Resonance Imaging ; Positron-Emission Tomography

By analysing working flow and layout of imaging department, the importance of scientific planning is elaborated. Also, how to relocate imaging department and facilities rapidly.

Liver sinusoid endothelial cells are the first defense mechanism that protects the liver against various injuries,and they also play a significant role in the development of liver fibrosis and liver cirrhosis caused by chronic liver injury.They are involved in liver fibrosis by mediating liver inflammation,participating in sinusoid capillarization and revascularization,activating hepatic stellate cells,secreting many proinflammatory cytokines,participating in extracellular matrix formation,and mediating liver microcirculation disturbance.Clarification of these mechanisms helps to identify new targets and develop new regimens for the treatment of liver fibrosis.

Objective To screen the proteins binding with HBV X-promoter and to investigate their potential role in the regulation of replication and expression of HBV DNA. Methods By using HBV X biotinylated promoter DNA as the selective molecule, the T7 select human liver cDNA library was biopanned and positive clones were selected. After screening, positive plaques were performed to amplify for inserted DNA fragment and cloned into pGEM-Teasy vector. Thirty positive plaques were chosen for DNA sequencing. Results Five kinds of known and nine kinds of novel cDNA sequences were obtained. The 5 kinds of known ones are human serum albumin, NADH dehydrogenase subunit 4, prokininase, ubiquitin specific protease 10, and testis enhanced gene transcript. Conclusion The binding proteins of HBV Xp DNA were identified by phage display. The results suggest that this approach provides a new search tool for the study of replication and expression mechanism of HBV DNA.

Objective To evaluate the construction of expression vector for fusion protein of cell-penetrating pep-tide CCL (PEP-CCL).Methods CCL6-PEP-6XHis was inserted into plasmid pABP,pABP-CCL6-PEP plasmid was extracted and then transfected into HEK293 cells,CCL6-PEP-6XHis was expressed and purified by chromatog-raphy and detected with Western Blot.Results PEP-CCL express vector was successfully constructed and purified. PCR product of CCL6-PEP-6XHis Tag was ligated with T vector,recombinant was transferred into the host cells, then host cells were cultured,plasmid was extracted and sequenced,the sequence was identical to targeted gene. CCL6-PEP-6XHis was successfully inserted into the eukaryotic expression vector pABP,plasmid was extracted and digested,electrophoresis results revealed that a fragment with 430bp was digested by Hind Ⅲ+XbaⅠ,which was identical to the expected value.Western Blot revealed that CCL6-PEP fusion protein could be recognized by His monoclonal antibody.Conclusion PEP-CCL express vector can be constructed and expressed in eukaryotic cells.

Objective To determine pharmacokinetics profiles of sodium alginate dexamethasone in perilymph after trans-round window administration of the sodium alginate dexamethasone gel in vivo.Methods 30 healthy guinea pig were randomly divided into 6 groups.Alg-Dex gel was placed into the niche of the round window of the guinea pig on the right ear.The perilymphetic samples were harvested on day 1,2,3,4 and 5 after administration of Alg-Dex gel respectively.The concentrations of dexamethasone in perilymph were assayed with high performance liquid chromatograph(HPLC).Results Concentrations of dexamethasone in perilymph on day 1,2,3,4 and 5 after administration of Alg-Dex gel were 0.49?0.06,1.32?0.28,0.65?0.08,0.66?0.05,0.53?0.17 mg/L respectively.Concentrations of dexamethasone in perilymph reached a peak on the second day and maintained 0.61?0.07 mg/L steadily on following days after administration.Conclusion Dexamethasone can be released from the sodium alginate dexamethasone gel persisitently and steadily in vivo.

OBJECTIVE: To determine Ginsenoside Rg1,Ginsenoside Re and Ginsenoside Rb1 in Yixin fumai granula by HPLC.METHODS: Samples were determined on a Diamond C18(150 mm?4.6 mm,5 ?m).The mobile phase consisted of accetonitrile-water by gradient elution with flow rate at 1 mL?min-1,UV detection wavelength at 203 nm,column temperature at 25 ℃ and sample size at 10 ?L.RESULTS: The linear ranges of Ginsenoside Rg1,Ginsenoside Re and Ginsenoside Rb1 were 0.645～6.450 ?g(r=0.999 8),0.54～5.40 ?g(r=0.999 7) and 0.605～6.050 ?g(r=0.999 9),respectively;the average recoveries were 100.59%(RSD=2.03%),98.70%(RSD=1.46%)and 98.99%(RSD=1.19%),respectively.CONCLUSION: The method is sensitive,simple and accurate,and it can be used for the quality control of Yixin fumai granula.

Objective To investigate the clinical efficacy and safety of montmorillonite powder combined with saccharomyces boulardii powder in treatment of acute diarrhea in children.Methods A total of 68 children with acute diarrhea from March 2014 to July 2015 in Lishui People’s Hospital were collected and randomly divided into experiment group and control group, with 34 cases in each group.Patients in control group were treated by montmorillonite on the basis of anti-inflammation, fluid infusion, support and other symptomatic treatment; patients in experiment group were treated saccharomyces boulardii powder combined with montmorillonite powder on the base of clinical routine treatment, the course was 6 days.The stool frequency, diarrhea control time, diarrhea total time and incidence of adverse reactions rate of two groups before and after treatment were observed, and the validity and safety were compared.Results After 6 days treatment, the daily stool frequency of experiment group were significantly lower than control group(P<0.05); diarrhea control time and diarrhea total time of experiment group were significantly lower than control group(P<0.05).The clinical efficacy of experiment group was 91.18%,higher than 73.53%of control group (P<0.05).Conclusion Saccharomyces boulardii powder combined with montmorillonite powder on acute diarrhea in children with good clinical efficacy.