By analysing working flow and layout of imaging department, the importance of scientific planning is elaborated. Also, how to relocate imaging department and facilities rapidly.

Cochlear Implants ; Magnetic Resonance Imaging ; Positron-Emission Tomography

Objective To investigate the possible role of eukaryotic initiation factor 6(eIF6)in the development of kidney inter-stitial tissue fibrosis in a mouse model of unilateral ureteral obstruction(UUO).Methods (1)UUO model was set up in eIF6+/-and eIF6+/+ mice.(2)HE and Masson staining were used to histologically assess the interstitial deposition of collagen in mouse kidney.Immunohistochemistry and image analysis were applied to analyze the expression of α-SMA and TGF-β1 in mouse kidney as well as eIF6 in human kidney samples.Results 10 days after unilateral ureteral obstruction,more kidney interstitial fibrosis tissues were found in eIF6+/- mice than that in eIF6+/+ mice.TGF-β1 andα-SMA were expressed more intensive in tubular epithelial cells of eIF6+/- mice than that of eIF6+/+ mice in the UUO model.Expression of eIF6 is lower in the region of fibrosis than that in non-fibrotic area.Conclusion eIF6 might be involved in the development of kidney interstitial tissue fibrosis by regulating the expres-sion of TGF-β1 .

Objective To explore the effect of methylprednisolone combined with interferon in the treatment patients with relapse remitting multiple sclerosis.Methods 90 patients with relapse remitting multiple sclerosis were randomly divided into experimental group and treatment group.The experimental group during the acute stage with methylprednisolone pulse therapy,remission with interferon β (IFN-β) treatment; the control group only in the acute phase with methylprednisolone pulse therapy.Results The total effective rate of methylprednisolone pulse therapy in the acute stage was 98.9％.Experimental group during the treatment with IFN-β1α,relapse rate was 30.2％ ;patients in the control group were followed up for two years,the recurrence rate was 53.3％.Experimental group and control group was significantly different ( P ＜ 0.05 ),the experimental group was significantly lower than the control group.Conclusion For relapsing-remitting MS,using MPPT could relieve acute symptom in the acute stage,and in remission using of IFN-β1α relapse prevention was a good choice for clinicians.

Congenital malformations of the external and middle ear is the common reason of pediatric hearing impairment and cosmic problem. The treatment composes of auricular plastic surgery and auditory reconstruction surgery. The use of BAHA, vibrant sound-bridge and tissue engineering materials can significantly improve the treatment outcomes.
Contraindications ; Ear, External ; abnormalities ; surgery ; Ear, Middle ; abnormalities ; surgery ; Hearing Aids ; Humans ; Infant, Newborn ; Reconstructive Surgical Procedures ; Treatment Outcome

Purpose:To analyze retrospectively the clinical manifestation, treatment and prognosis. Methods:From 1978 to 1996,16 patients with olfactory neuroblastoma were treated.4 cases were stage A,6 cases were stage B and 6 cases were stage C. Six patients were treated with radiotherapy alone or combined with chemotherapy, 9, with surgery and postoperative radiotherapy and 1,with operative alone. The radiation dosage ranged from 40～70Gy with 2 Gy per day. Results:The 1 , 3 and 5 year survival rates were 75%, 75% and 69%.Conclusions:Olfactory neuroblastoma is sensitivity for radiotherapy, for the patients with stage A, radiotherapy alone is effective for stage B and C olfactory neuroblastoma , combined modality of surgery and postoperative radiotherapy should be the treatment of choice.

Liver sinusoid endothelial cells are the first defense mechanism that protects the liver against various injuries,and they also play a significant role in the development of liver fibrosis and liver cirrhosis caused by chronic liver injury.They are involved in liver fibrosis by mediating liver inflammation,participating in sinusoid capillarization and revascularization,activating hepatic stellate cells,secreting many proinflammatory cytokines,participating in extracellular matrix formation,and mediating liver microcirculation disturbance.Clarification of these mechanisms helps to identify new targets and develop new regimens for the treatment of liver fibrosis.

Objective It has been shown that fluorinated inhalational anesthetics have various adverse effects on the lungs. The aim of this study was to investigate the effects of different concentrations of isoflurane (ISO) on the lungs in rats.Methods Ninety Wistar rats of both sexes weighing 140-200 g were randomly divided into two groups : (A) control group received only oxygen inhalation ( n = 30) and (B) isoflurane group (n = 60) which was farther divided into 2 subgroups (n = 30):0.6% and 1.4% isoflurane. In each subgroup isoflurane was inhaled for 2 h ( n = 10), 4h (n = 10) or 8 h ( n = 10) . The animals were placed in a glass container and isoflurane was delivered from ISO vaporizer into the container through the inlet. The end-tidal ISO concentration was checked at the outlet. The animals were sacrificed at the end of ISO inhalation. The lungs were immediately removed and blood was collected for determination of (1) lung water content, (2) protein content and neutrophil ratio in broncho-alveolar lavage fluid (BALF) , (3) serum and BALF surfactant protein-A (SP-A) content and (4) microscopic examination. Results There was no significant difference in all variables between control group and 0.6% ISO subgroup. Exposure to 1.4% ISO for 8 h caused an increase in neutrophil ratio and protein content in BALF, and serum SP-A content but a decrease in BALF SP-A content. There was no significant difference in lung water content between control group and 1.4% ISO (8 h) subgroup. Conclusion Isoflurane (1 MAC) inhalation over 8 h may impair the function of alveolar epithelium.

Objective To evaluate the construction of expression vector for fusion protein of cell-penetrating pep-tide CCL (PEP-CCL).Methods CCL6-PEP-6XHis was inserted into plasmid pABP,pABP-CCL6-PEP plasmid was extracted and then transfected into HEK293 cells,CCL6-PEP-6XHis was expressed and purified by chromatog-raphy and detected with Western Blot.Results PEP-CCL express vector was successfully constructed and purified. PCR product of CCL6-PEP-6XHis Tag was ligated with T vector,recombinant was transferred into the host cells, then host cells were cultured,plasmid was extracted and sequenced,the sequence was identical to targeted gene. CCL6-PEP-6XHis was successfully inserted into the eukaryotic expression vector pABP,plasmid was extracted and digested,electrophoresis results revealed that a fragment with 430bp was digested by Hind Ⅲ+XbaⅠ,which was identical to the expected value.Western Blot revealed that CCL6-PEP fusion protein could be recognized by His monoclonal antibody.Conclusion PEP-CCL express vector can be constructed and expressed in eukaryotic cells.

Objective To investigate the clinical efficacy and safety of montmorillonite powder combined with saccharomyces boulardii powder in treatment of acute diarrhea in children.Methods A total of 68 children with acute diarrhea from March 2014 to July 2015 in Lishui People’s Hospital were collected and randomly divided into experiment group and control group, with 34 cases in each group.Patients in control group were treated by montmorillonite on the basis of anti-inflammation, fluid infusion, support and other symptomatic treatment; patients in experiment group were treated saccharomyces boulardii powder combined with montmorillonite powder on the base of clinical routine treatment, the course was 6 days.The stool frequency, diarrhea control time, diarrhea total time and incidence of adverse reactions rate of two groups before and after treatment were observed, and the validity and safety were compared.Results After 6 days treatment, the daily stool frequency of experiment group were significantly lower than control group(P<0.05); diarrhea control time and diarrhea total time of experiment group were significantly lower than control group(P<0.05).The clinical efficacy of experiment group was 91.18%,higher than 73.53%of control group (P<0.05).Conclusion Saccharomyces boulardii powder combined with montmorillonite powder on acute diarrhea in children with good clinical efficacy.