Hypereosinophilir. dermatitis, which was first described in 1981, is a clinically distinet disease within the spectrum of the hypereosinophilic syndrome. It is characterized by a generalized polymorphous pruritic eruptions and blood eosinophilia with minimal or no extracutaneous involvement known cause hypereosinophilia, namely parasites, allergies and drug association are absent in these patients. We present a patient with hypereosinophilic dermatitis who did not show evidence of systemic disease. The patient was a 18-year old male who had pruritic erythematous brownish elevated plaque on both lower extremities for 5 months. A)though the patients skin lesions improved after treatment with steroids and dapsone, after discontinuing medication, hypereosinophilia recurred.
Adolescent ; Dapsone ; Dermatitis* ; Eosinophilia ; Humans ; Hypereosinophilic Syndrome ; Hypersensitivity ; Lower Extremity ; Male ; Parasites ; Skin ; Steroids

No abstract available.
Sarcoma*

For the more practical and convenient application of the Tzanck test, we evaluated several staining methods and cytological findings 98 cases of vesicobullous skin diseases. Fixation by air-drying of specimen aad modified Giemsa staining method were thought to be the most appropriate procedure in clinical use. The disease studied were chickenpox, herpes zoster & simplex, contact dermatitis, Id reaction, tinea pedis, urticaria pigmentosa, incontinentia pigmenti, farnilial benign pemphigus, hand-foot-mouth disease, erytherna multiforme, toxic epiderrzal necrolysis, fixed drug eruption, and septic vesicle. Among these, herpes and chickenpox vesicles shawed the most characteristic and specific findings. And degenerating giant cell could be added as an useful diagnostic marker for these diseases in relatively old lesions. In the other diseases, cytological findings were neither charcteristic nor specific. But these findings suggested the possibility of picking up useful findings by more elaborated study including more large number of cases.
Azure Stains ; Chickenpox ; Dermatitis, Contact ; Drug Eruptions ; Giant Cells ; Herpes Zoster ; Incontinentia Pigmenti ; Pemphigus ; Skin Diseases* ; Skin* ; Tinea Pedis ; Urticaria Pigmentosa

PURPOSE: We intended to check the growth rates and phenotypic markers of chondrocytes in the dedifferentiated cells cultivated in various conditions in order to establish the ideal culture system for implantation. MATERIALS AND METHODS: Culturing rabbit chondrocytes from proximal tibia, we checked the phenotypes at first, second, and third week. Then we cultured the chondrocytes in different circumstances such as monolayer or three dimensional gel in the presence or abscence of TGF-B1, and checked the growth rates and phenotypic markers. RESULTS: There was no difference in growth rates and mRNA level of type I, type II collagen and aggrecan between the cells cultured in monolayer and three dimensional gel of collagen. However, the responses of the cells to TGF-B1, were quite different between these two groups. In monolayer culture, the expression of type I collagen was depressed by TGF-B1 while the growth rate was markedly increased. Oppositely in three dimensional culture, the mRNA level of type I collagen was markedly increased and the growth rate was completely suppressed by TGF-B1. The expression of type II collagen could be detected only in TGF-B1-treated cells cultured in three dimensional gel for 4 or more days. The mRNA level of aggrecan was also increased by TGF-B1, in the cells cultured in three dimensional gel. CONCLUSIONS: These results suggest that the number of chondrocytes can be efficiently expanded by culturing the cells in monolayer and the phenotypes of chondrocyte can be restored by culturing the cells in three dimensional gel containing TGF-B1. The application of semi-solid gel containing differentiated chondrocytes in physeal implantation should be further evaluated
Aggrecans ; Chondrocytes* ; Collagen ; Collagen Type I ; Collagen Type II ; Phenotype ; RNA, Messenger ; Tibia ; Transforming Growth Factor beta1*

The Marjolin's ulcer is the malignant lesion developed in a burn scar or chronic fistula. Due to the low prevlalence of this lesion, there has been disagreement in its clinical features, methods of treatment, and prognosis. We evaluated 19 cases of patients who had been admitted to Severence Hospital from Jan. 1970 to Dec. 1958. The results were as follows : 1. The previous lesion was burn scar in 52% of the cases and fistula of chronic osteomyel-itis in 32%. 2. The mean latent period was 31.5 years. 3. The initial symptoms were increased pain(74%), discharge with foul odor(68%) and bleeding(58%). 4. Upon histological examination, all of the cases were squamous cell carcinoma. 5. The rate of recurrence of metastasis at time of diagnosis was 32%. 6. Of the 16 patients treated by surgery, local recurrence was noted in 4 cases. Three of these cases were patients who had been treated by excision and split thickness skin graft. 7. The time interval for local recurrence ranged from 6 months to 11 months(average 8. 8 months). In conclusion, the squauious cell carcinoma of the Marjolin's ulcer seems to have a worse prognosis than other squamous cell carcinoma and it requres aggressive treatment. The burn scar or chronic fistula that occured in elderly patients especially required more adequste treatment and close observation.
Aged ; Burns ; Carcinoma, Squamous Cell ; Cicatrix ; Clinical Study ; Diagnosis ; Fistula ; Humans ; Neoplasm Metastasis ; Prognosis ; Recurrence ; Skin ; Transplants ; Ulcer

Adenoids ; Carcinoma, Squamous Cell ; Diagnosis ; Female ; Hemorrhage ; Humans ; Male ; Mouth ; Mouth Neoplasms ; Neck Dissection ; Paralysis ; Paresthesia ; Pigmentation ; Suppuration ; Surgery, Oral ; Survival Rate ; Trismus ; Ulcer

63 of 348 patients patch tested with an European standard series, had positive reaction to either a fragrance mix or Balsam of Peru. Twelve showed positiv reaction to bot,h patches. The number of positive patch tests to the fragrance mix was 56, while those to Balsarn of Peru was 19 out, of the 63 patients, manifesting sensitivities of 83% and 30% repectively. The fragrance mix was considered a useful screening patch for fragrance allergies. Twenty-five of the patients showing fragrance allergy were further analysed with 8 individual fragrance ingredients at 2% petrolatum. Cinnamic aldehyde and cinnamic alcohol were considered as she most frequent and important. sensitizers.
Humans ; Hypersensitivity* ; Mass Screening ; Patch Tests* ; Peru ; Petrolatum

A 33 years old house wife with accessory breast tissue is described and the literature is reviewed. The patient developed soft tumors in both axillae with axillary hair loss during the 8 th month of her 8 th pregnancy. This disorder is rather common in the literature but has been paied little attention dermatology.
Adult ; Axilla ; Breast* ; Dermatology ; Hair ; Humans ; Pregnancy ; Spouses

The aims of this experiment were to investigate the strain and temperature changes simultaneously within autopolymerizing acrylic resin specimens. A computerized data acquisition system with an electrical resistance strain gauge and a thermocouple was used over time periods up to 180 minutes. The overall strain kinetics, the effects of stress relaxation and additional heat supply during the polymerization were evaluated. Stone mold replicas with an inner butt-joint rectangular cavity (40.0x25.0mm, 5.0mm in depth) were duplicated from a brass master mold. A strain gauge (AE-11-S50N-120-EC, CAS Inc., Korea) and a thermocouple were installed within the cavity, which had been connected to a personal computer and a precision signal conditioning amplifier (DA 1600 Dynamic Strain Amplifier, CAS Inc., Korea) so that real-time recordings of both polymerization-induced strain and temperature changes were performed. After each of fresh resin mixture was poured into the mold replica, data recording was done up to 180 minutes with three-second interval. Each of two poly (methyl methacrylate) products (Duralay, Vertex) and a vinyl ethyl methacrylate product (Snap) was examined repeatedly ten times. Additionally, removal procedures were done after 15, 30 and 60 minutes from the start of mixing to evaluate the effect of stress relaxation after deflasking. Six specimens for each of nine conditions were examined. After removal from the mold, the specimen continued benchcuring up to 180 minutes. Using a waterbath (Hanau Junior Curing Unit, Model No.76-0, Teledyne Hanau, New York, U.S.A.) with its temperature control maintained at 50degrees C, heat-soaking procedures with two different durations (15 and 45 minutes) were done to evaluate the effect of additional heat supply on the strain and temperature changes within the specimen during the polymerization. Five specimens for each of six conditions were examined. Within the parameters of this study the following results were drawn : 1. The mean shrinkage strains reached -3095mu epsilon, -1796mu epsilon and -2959mu epsilon for Duralay, Snap and Vertex, respectively. The mean maximum temperature rise reached 56.7degrees C, 41.3degrees C and 56.1degrees C for Duralay, Snap, and Vertex, respectively. A vinyl ethyl methacrylate product (Snap) showed significantly less polymerization shrinkage strain (p<0.01) and significantly lower maximum temperature rise (p<0.01) than the other two poly (methyl methacrylate) products (Duralay, Vertex). 2. Mean maximum shrinkage rate for each resin was calculated to ?31.8mu epsilon/sec, -15.9mu epsilon/sec and ?31.8mu epsilon/sec for Duralay, Snap and Vertex, respectively. Snap showed significantly lower maximum shrinkage rate than Duralay and Vertex (p<0.01). 3. from the second experiment, some expansion was observed immediately after removal of specimen from the mold, and the amount of expansion increased as the removal time was delayed. For each removal time, Snap showed significantly less strain changes than the other two poly (methyl methacrylate) products (p<0.05). 4. During the external heat supply for the resins, higher maximum temperature rises were found. Meanwhile, the maximum shrinkage rates were not different from those of room temperature polymerizations. 5. From the third experiment, the external heat supply for the resins during polymerization could temporarily decrease or even reverse shrinkage strains of each material. But, shrinkage re-occurred in the linear nature after completion of heat supply. 6. Linear thermal expansion coefficients obtained from the end of heat supply continuing for an additional 5 minutes, showed that Snap exhibited significantly lower values than the other two poly (methyl methacrylate) products (p<0.01). Moreover, little difference was found between the mean linear thermal expansion coefficients obtained from two different heating durations (p>0.05).
Acrylic Resins* ; Electric Impedance ; Fungi ; Heating ; Hot Temperature ; Kinetics ; Microcomputers ; Polymerization* ; Polymers* ; Relaxation

A 62-year-old man presented with a tender, solitary nodule on the left upper arm. He had no specific family history of the lesion. A biopsy specimen showed various sized irregular vascular lumens in the upper dermis. The vascular walls consisted of an endothelial cell layer and rim of one to three layers of glomus cell nests outside the endothelial cells. The cell nests consisted of round shaped epitheloid cells with relatively uniform round or oval shaped nuclei and pale cytoplasm. These tumor cells were strongly positive for vimentin, and a-smooth muscle actin. On electron microscopic examination, the cytoplasm of these tumor cells contained fine filamentous components and many electron dense bodies were found at the plasma membrane and the cytoplasm. We report a case of a solitary glomus tumor(glomangioma) that showed the histological features of the multiple type of glomus tumor. In addition we describe the results of an immunohistochemical and electron microscopic study.
Actins ; Arm ; Biopsy ; Cell Membrane ; Cytoplasm ; Dermis ; Endothelial Cells ; Glomus Tumor ; Humans ; Middle Aged ; Vimentin