Objective:To investigate the expressions of P27kipl (P27) protein and cyclin E protein in human renal cell carcinoma and in paracarcinoma kidney cortex tissue,and to explore the correlation of these protein expressions with biological behavior of tumors.Methods:P27 and cyclin E expressions were studied in formalin-fixed,paraffin-embedded sections of renal cell carcinoma and in paracarcinoma kidney cortex tissue by means of a specific and sensitive immunohistochemical S-P assay.Results:The positive rates of P27 protein and cyclin E protein in renal cell carcinoma were significantly higher than those in paracarcinoma kidney cortex tissues ( P

Objective To supervise the colonoscopy following up in the high risk colorectal cancer patients are good to find out early stage cancer and adenoma in time and prevent the latter to evolve in to cancer. Methods From Aug. 1995 to Mar. 2000, 1 125 cases of high risk colorectal cancer have received the colonoscopy .Of them, 353 cases who have symptoms after having the physical examination in clinics; 125 cases have the colorectal cancer family history without any symptoms; 386 cases have had operated on the colorectal cancer; and 261 cases with the colorectal adenoma after high frequency electrotomy under endoscopy in one year. Results In 353 cases, 52 cases (14.7%) with adenoma and 17 cases (4.8%) with colorectal cancer are found, and 12 cases (70.5%)of the latter are of early stage cancer without any lymph node metastasis. Among the 125 cases there were 26 cases (21.6%) with adenoma, 4 cases (3.2%) with colorectal cancer and 2 cases (1.6%) with adenoma early stage cancer. Among the latter 6 cases, 5 cases are of the early stage cancer without any lymph node metastasis. Of the 386 cases , 81 cases (20.9%) of adenoma, 3 cases (0.7%) of the third primary cancer, and 11 cases (2.8%) of second primary cancer were found in which 11 cases (78.5%) are of the early stage cancer without any lymph node metastasis. In 261 cases there were 56 cases (21.4%) with adenoma, among them, 41 cases (73.2%) are the newly found adenoma with diameter 1cm in diameter were missed in the first colonoscopy.Conclusion A total of 1 125 cases with high risk colorectal cancer were examined by colonoscopy, it was found 37 cases (3.3%) with carcinoma, in which 28 cases (75.7%) with early cancer of no lymph node metastasis, and other 215 cases (19.1%) with adenoma.

Objective To evaluate the safety and feasibility of minimally invasive percutaneous nephrolithotomy (mPCNL) for the treatment of upper urinary tract calculi. Methods From October 2003 to December 2006, mPCNL was performed on 127 patients suffering from upper urinary tract calculi by using Karl Storz F8/9.8 rigid ureteroscope and homemade pneumatic ballistic lithotriptor. Results Of the 127 cases, mPCNL was completed in 125, and was converted to open surgery in 2. The rate of stone removal was 89.8% (114/127). The operation time was 78-185 min (mean 108 min). Nephrostomy tube was kept for a mean of 7 d (5-11 d). The mean hospital stay was 8.1 d (5-12 d). Two patients received blood transfusion during the operation. One patient developed massive hemorrhage at the nephrostomy opening, and was cured by high-selective renal artery embolization. Among the patients, 117 were followed up for 1-16 months (mean 5.3), during which no recurrent urinary tract stones was found by B-ultrasonography or X-ray. Conclusions By using mPCNL, patients with upper urinary tract calculi can be treated safely and effectively with a high rate of stone removal, less trauma, quick recovery, and short hospital stay.

Objective To investigate the recognition of graded nursing and its influencing factors among nursing assistants in elder homes.Methods A questionnaire survey was conducted on 116 nursing assistants.Results The nursing assistants showed insufficient recognition of graded nursing,there were significant differences in role cognition,operation and practice training,salary,and the nursing workload,there were no significant differences in working years and educational levels.Conclusions The recognition of graded nursing was insufficient.It is suggested to increase the role cognition education,enhance the theory and practice training,provide higher salaries and allocate workload reasonably to ensure the implementation of graded nursing and increase the satisfaction level of the elderly.

Integrin ?V?3, one of the major members of the integrin family, plays a pivotal role in tumor angiogenesis because it mediates the cell-extracellular matrix and cell to cell adhesion as well as controls cells proliferation, differentiation, cellular modality and motility.Due to restricted high-expressions in tumors, integrin ?V?3, is considered a suitable targeting receptor for tumor diagnosis.In the past decade, several molecular imaging tracers for integrin ?V?3, have been used to noninvasively visualize its expression in tumor tissues and angiogenic blood vessels through the usage of nuclear imaging, nuclear magnetic resonance imaging, ultrasound, and optical imaging.Noninvasive detection of integrin ?V?3, expression can potentially be used for tumor diagnosis and prognosis.The current imaging probes used to target integrin ?V?3, through usage of different technologies are briefly recapitulated in this review.

Objective To study the expressions of p27Kip1 (p27) protein and Ki 67 antigen in human clear cell carcinoma of kidney and to find out the relationship between the expression levels and the clinical pathological characteristics and prognosis in cases of clear cell carcinoma of kidney. Methods The expressions of p27 protein and Ki 67 antigen in the adjacent tissues of carcinoma in 20 cases and 42 cases of clear cell carcinoma of kidney were detected by immunohistochemistry. Results The positive rates of p27 protein and Ki 67 antigen in clear cell carcinoma of kidney were significantly higher than those of the both in the adjacent tissues of carcinoma( P

Objective:To study adenovirus mediated transfer of TGF ?1 in treatment of mice autoimmune myocarditis. Methods: Experimental autoimmune myocarditis was induced in BALB/c mice using purified myosin from porcine heart. TGF ?1 was transferred into mice by intravenous injection of adenovirus encoding TGF ?1. Plasma level of myosin autoantibody was detected by indirect ELISA method and plasma level of troponin I was assayed using fluorescence immune assay method. The protective effects of TGF ?1 were evaluated from 3 aspects: (1) the degree of infiltrative inflammatory cells in the heart; (2) the plasma level of myosin autoantibody; (3) the plasma level of troponin I. Results: TGF ?1 successfully ameliorated the myocarditis. Plasma level of troponin I and myosin autoantibody decreased significantly( P

Objective To provide anatomical basis for one-stage full dynamic correction of late facial palsy with double latissimus dorsi segmental muscle flaps.MethodsForty sides of formaldehyde fixed latissimus dorsi in twenty cadavers were selected to microsurgery anatomy,three sides fresh latissimus dorsi specimens were blood vessel casted.The extramusclar and intramuscular neurovascular distribution were observed.Results①In 92.5%of the thoracodorsal nerve divides into a medial and a lateral branch be fore entering the muscle.In 7.5%of the thoracodorsal nerve divides into three major branches.The coordinate of the bifurcation point of the thoracodorsal Here was[(7.94±1.23)cm,(3.71±1.68)cm].In the district of this angle's middle line,the numbers of vessels and nerves is relative few.②The lateral muscle flaps of the latissimus dorsi can be divided into 3-5 independent segmental flaps,the medial muscle flaps of the latissimus dorsi Can be divided into 2-4 independent segmental flaps.③The segmental nerve usually locate the two sides of vessel,which are arranged(from medial to lateral)in a NVAV order in the reedial segmental branches(100%),in a VAVN order in the lateral segmental branches(85.0%)and in a NVAV order in the lateral segmental branches(15.0%).④The neural pedicle Was cut from the lateral bifurcation point,the mean length of the third medial muscle neural pedicle was 16 cm；the mean length of the third or the forth lateral muscle neural pedicle was 12 cm.Conclusion Late facial palsy can be cuwd with onestage cross-facial transplantation of neurovascularized free double latissimus dorsi segmental muscle flaps.

Objective To investigate the nutrition support during stress response period after tumor ex-tirpation in middle-aged and elderly patients with oral and maxillofacial tumors. Methods Sixty patients who had undergone maxillofacial tumor extirpation were randomly divided into nutrition therapy group (n = 35) and control group ( n = 25 ). Patients in nutrition therapy group received enteral nutritional treatment, while patients in control group fed themselves. The nutritional status of the two groups before operations were evaluated with Concise Nutritional Assessment Indicators of human body measurement and biochemistry were used to evaluate the nutritional conditions. The incidence of post-operational complications and recovery time ( from the operation to discharge) were analyzed. Results The weight and arm muscle circumference in the nutrition therapy group significantly decreased after operations (P <0. 05). However, in the control group, all the nutritional indica-tors except the total lymphocyte count were significantly lower than the baseline levels after operations (P < 0. 05 ) ; particularly, of the average body weight decreased by about 6 kg. The incidence of complications in the nutrition therapy group was significantly lower than that in control group (5. 7% vs. 12. 0% , P <0. 05). The recovery time was significantly longer in the control group than that in the nutrition therapy group [ (28. 62± 2.38) vs. (22. 76± 3. 24) d, P < 0. 01 ]. Conclusion The nutritional support during the stress response period can satisfy the demands of high catabolism after maxillofacial tumor extirpation operation in middle-aged and elderly patients.

Accidents, Occupational ; Acetic Anhydrides ; Adult ; Blast Injuries ; complications ; therapy ; Burns, Inhalation ; etiology ; therapy ; Humans ; Lung Injury ; etiology ; therapy ; Male ; Middle Aged