Objective:To explore the clinical value of color Doppler ultrasound and multi-slice spiral computed tomography (MSCT) in the diagnosis of pulmonary thromboembolism (PTE)- deep vein thrombosis (DVT).Methods: A retrospectively study was adopted to analyze 30 patients with suspected PTE-DVT. The patients with suspected PTE were applied by using MSCT and series three-dimensional reconstructions, such as volume reconstruction (VR), multi planar reconstruction (MPR), maximum intensity projection (MIP) and axial image reconstruction, while the patients with suspected DVT were scanned by using color Doppler flow imaging (CDFI). The shape, size and location of embolus in blood vessel were observed, and the situations of pulmonary artery embolism and the phenomenon of deep vein embolism detected by the two methods were compared and analyzed.Results: In 30 cases, there were 15 cases were diagnosed as positive patients, and in the 15 cases, the patients with DVT were 66.7% (10/15), while the patients with PTE were 86.7% (13/15), and the patients with PTE-DVT were 8 cases (53.3%, 8/15). By using MSCT, there were 10 patients with thromboembolism in pulmonary artery were found, and there were 50 pulmonary arteries with thromboembolism were diagnosed. On the other hand, by using color Doppler ultrasound, there were 12 patients with embolism and fluctuation disappearing of vein wall in deep vein were found, and there were 16 deep veins with thromboembolism were diagnosed.Conclusion: Color Doppler ultrasound should combine with MSCT and they should learn each other so as to achieve the diagnosis of PTE-DVT in convenient, fast, simple, safety and accuracy. This combination of them can provide important reference for clinical therapy of PTE-DVT.

OBJECTIVE: To analyze the relationship between female resting energy expenditure and obesity-related indicators, and to compare the differences of resting energy expenditure and resting energy expenditure per kilogrem between obese women and normal weight women.METHODS: A total of 565 healthy women who did medical examination or visited Department of Endocrinology in Beijing Obstetrics and Gynecology Hospital between 2004 and 2008 were recruited, their height and weight were measured. Human metabolic apparatus was used to measure resting energy expenditure, then body surface area, body mass index, resting energy expenditure per kilogram were calculated. According to the diagnostic cdtada of obesity by body mass index, the 565 cases were divided into two groups: obese group (n=179) and normal body weight group (n=240), and all the measurement results were calculated and did the tests of normality, correlation analysis and two independent samples rank sum test or t test. RESULTS: The resting energy expenditure of 565 healthy women were normality, the result was (5.793±0.940) k J/d, and resting energy expenditure showed a positive correlation with body weight, height, body mass index and body surface area (P < 0.05), while showed no significant correlation with age (P > 0.05), there was no significant difference of age end height between obese group and normal body weight group (P > 0.05), while a statistical significant difference was found in resting energy expenditure and resting energy expenditure per kilogram between obese group and normal body weight group (P > 0.05).CONCLUSION: There is a positive correlation that female resting energy expenditure with their height, weight, body mass index and body surface area, and the resting energy expenditure of obese women is higher than that of normal body weight women, while resting energy expenditure per kilogram of obese women is lower than that of normal body weight women.

Ascomycota ; isolation & purification ; pathogenicity ; Female ; Humans ; Respiratory Hypersensitivity ; microbiology ; Sinusitis ; microbiology ; Young Adult

OBJECTIVE:To explore the effects of recombinant adenovirus vector Adxsi-GFP-VP3 carrying apoptin gene VP3 on the apoptosis of human lung squamous carcinoma SK-MES-1 cell lines and human lung adenocarcinoma NCI-H1299 cell lines. METHODS:The exponential phase SK-MES-1 and NCI-H1299 cell lines were respectively divided into a recombinant adenovirus (Adxsi-GFP-VP3) group,a empty virus (Adxsi-GFP) group and a cell control (phosphate buffer) group,which were marked as group A,B and C respectively. Reverse transcription polymerase chain reaction and Western blot method were used to detect the ex-pressions of VP3 mRNA and Apoptin in the cells of groups A and B 48 and 72 h after transfection. The change in the ultrastructure of the cells in group A was observed under transmission electron microscope 72 h thereafter. MTT method was adopted to detect the cell proliferation activities of three groups 24,48,72 and 96 h thereafter and flow cytometry to determine the apoptosis rates and cell cycle changes 24,48 and 72 h thereafter. RESULTS:Compared to group B,group A demonstrated the expression of VP3 mRNA in SK-MES-1 and NCI-H1299 cell lines 48 h after transfection,and Apoptin expression and ultrastructure change for apopto-sis of SK-MES-1 and NCI-H1299 cell lines 72 h thereafter. Compared to groups B and C,group A showed lower proliferation activ-ities and higher apoptosis rates of SK-MES-1 and NCI-H1299 cell lines,which had a positive correlation with transfection time;and in the group A,there was a decrease in the proportion of the SK-MES-1 and NCI-H1299 cell lines in S phase and an increase in the proportion of those in G2/M phase,72 h after transfection. There was statistically difference (P<0.05). CONCLUSIONS:Adxsi-GFP-VP3 can effectively induce the apoptosis of SK-MES-1 and NCI-H1299 cell lines.

Lipoma preferred partner (LPP) has been identified as a protein which is highly selective for smooth muscle progenitor cells (SMPCs) and regulates differentiation and migration of SMPCs, but mechanisms of LPP expression are not elucidated clearly. The aim of the present study was to discuss the mechanisms by which LPP expression is regulated in the differentiation and migration of SMPCs induced by TGF-β1. It was found that TGF-β1 could significantly increase the expression of LPP, smooth muscle α-actin, smooth muscle myosin heavy chain (SM-MHC), and smoothelin in SMPCs. Moreover, inactivation of Rho kinase (ROK) with ROK inhibitors significantly inhibited LPP mRNA expression in TGF-β1-treated SMPCs and mouse aortic smooth muscle cells (MAoSMCs). At the same time, LPP silencing with short interfering RNA significantly decreased SMPCs migration. In conclusion, LPP appears to be a ROK-dependant SMPCs differentiation marker that plays a role in regulating SMPCs migration.

Objective To investigate the role of HMGB 1 in epithelial-mesenchymal transition .Methods Specific siRNA of HMGB1 was designed and synthesized .Cultured typeⅡalveolar epithelial cell line-A549 cells were divided into 4 groups:1)control group, 2)model group induced by TGF-β1, 3)HMGB1 RNAi group, 4)RNAi negative con-trol group .Cellular morphology changes were observed by phase-contrast microscope .HMGB1 andα-SMA expression in A549 cells was detected by RT-PCR and Western blotting respectively .Results mRNA and protein expression of HMGB1 andα-SMA in TGF-β1 group increased significantly than that in control group (P<0.01).HMGB1,α-SMA mRNA and protein expression in siRNA-treated cells decreased significantly as compared with that in TGF -β1 group (P<0.01).Conclusions HMGB1 may be involved in the TGF-β1 induced EMT.

Objective To evaluate the clinical efficacy and safety of high-dose Ambroxol in treatment of acute exacerbation of chronic obstructive pulmonary disease (AECOPD). Methods Retrieved from PubMed, EMbase, Cochrane Library, CNKI, VIP and WanFang databases by computer, randomized controlled trials (RCTs) about high-dose of Ambroxol in treatment of AECOPD were included. According to the inclusive and exclusive criteria, literatures and data were independently screened and extracted by two researchers, and the quality of the studies were assessed, Meta-analysis was conducted by RevMan 5.2 software. Results A total of 7 RCTs were included, involving 534 patients. Meta-analysis showed that experiment group can obviously improve the overall effective rate [OR = 4.11, 95%CI (2.31, 2.31), P < 0.000 01), shorten hospital stays(OR = 3.60, 95% CI (4.66, 2.55), P < 0.00001), compared with the control group was statistically difference, and no obvious adverse reactions. Conclusion High-dose of Ambroxol could improve AECOPD overall effective rate, shorten hospital stays, improve pulmonary function and blood gas analysis, and no serious adverse reactions, be worth clinical promote using.

Objective To investigate the association of blood transfusion volume with early death among recipients with known duration of infection in rural central China. Methods From January 1, 1999 to December 30, 2003, 76 individuals with duration of blood transfusion who met the study criterion had been found to be infected in the HIV/AIDS notified system in rural of Yuncheng Prefecture of Shanxi Province, China. A retrospective study was conducted to investigate Socio-demographic characteristics, transfusion volume, and mortality. Cox' proportional hazard regression model was used to analyze the data. Results By December 30, 2003, among 76 subjects with transfusion-acquired HIV infection, 24 recipients had died. Mortality rate and median survival was 45.64 and 8.3 per 1000 person years, respectively. Transfusion volume (HR=3.12, 95% CI: 1.18～8.25; P=0.0218) was significantly associated with mortality in recipients in the multiply Cox' proportional hazard regression model. Conclusions This study suggests that more units of transfusion in recipients with known duration of HIV infection can influence time to death in recipients in China.

Antimetabolites, Antineoplastic ; pharmacology ; Apoptosis ; Breast Neoplasms ; genetics ; metabolism ; pathology ; Cell Line, Tumor ; Drug Resistance, Neoplasm ; Female ; Fluorouracil ; pharmacology ; Gene Expression Regulation, Neoplastic ; Humans ; Inhibitor of Apoptosis Proteins ; Microtubule-Associated Proteins ; biosynthesis ; genetics ; physiology ; Neoplasm Proteins ; biosynthesis ; genetics ; physiology ; Oligodeoxyribonucleotides, Antisense ; genetics ; RNA, Messenger ; biosynthesis ; genetics ; Transfection

Child ; Child, Preschool ; Female ; Humans ; Infant ; Infant, Newborn ; Male ; Respiratory Tract Diseases ; diagnostic imaging ; Tomography, Spiral Computed ; methods