Objective To investigate the methods of repair of the excessive width,theatrical fold line or multiple eyelid following blepharoplasty.Methods From Mar.2005 to Dec.2013,129 patients received repair because of their excessive width,theatirical fold line or multiple eyelid after blepharoplasty.Among them,75 cases were repaired 2 times,43 cases were repaired 3 times,7 cases were repaired 4 times and 4 cases were repaired 5-7 times before this operation.The case history ranged from 6 months to 12 years.The orbital septum fat flap,orbital free fat graft and orbital filling were utilized.Results The repair results were very satisfactory in 87 cases (67.4 ％),satisfactory in 32 cases (24.8％),basically satisfactory in 7 cases (5.4％) and not satisfactory in 3 cases (2.3％).Conclusions It is a simple and effective approach that orbital septum fat flap,orbital free fat graft and orbital filling are used to repair the excessive width,theatrical fold line and multiple eyelid.

Effects of oleanolic acid on immunoregulation were investigated in mice on the molecular level. Results showed that oleanolic acid could stimulate the biosynthesis or release of prostaglandins E2, F2? ( PGE2, PGF2? ) and cAMP, whereas had an inhibitory action on cGMP and histamine. Prostaglandins, cyclic nucleotides and histamine participate in many bodily functions, including regulation of blood pressure, immune function. It is suggested that their interaction may be of potential importance as a possible mechanism by which oleanolic acid acts in disease.

Experimental studies were made on enhancing effect of aloperine on PG-cyclooxyge-nase activity. Radioimmunoassay for determination of PGE2 and PGF2? contents was employed to evaluate PG-cyclooxygenase activity. Mice were given aloperine (50mg ? kg ? d-1) ip for 7d and the effects of lung and kidney homogenates on extrogenous arachidonic acid were studied. The results suggest that biosynthesis of PGE2, PGF2? be obviously increased in the experimental than that of control, suggesting of aloperine showing enhancing effect on PG-cyclooxygenase activity.

Animals ; Bone and Bones ; metabolism ; Free Radicals ; metabolism ; Male ; Muscle, Skeletal ; metabolism ; Physical Conditioning, Animal ; physiology ; Rats ; Rats, Wistar

China ; Dyslipidemias ; prevention & control ; Guidelines as Topic ; Humans

s Objective To observe the enhancement of sensitivity of human gastric cancer cell MKN-74 to anticancer drugs by administration of 5-aza-2'-deoxycytidine(5-Aza-CdR)and trichostatin A(TSA)with anticancer drugs.Methods Growth inhibition of MKN-74 with treatment of 5-Aza-CdR and/or TSA together with anticancer drugs,including fluorouracil(5-FU),paclitaxel(PTX),oxaliplatin(OXA),SN38 or gemcitabine(GEM),was detected by MTT method.Induction of apoptosis was analyzed by flow cytometry.Expressions of p21,caspase3,Rb and p16 gene were studied by RT-PCR.The gray scale of the genes was analyzed by gel analysis software Bandscan 4.3,and the ratio of gray scale of targeted genes and GAPDH gene were calculated.Results Compared with 5-Aza-CdR alone,combined administration of 5-Aza-CdR and TSA enhanced the chemosensitivity of MKN-74 to 5-FU,PTX,OXA and GEM.Compared with TSA alone,the combined administration enhanced its osensitivity to OXA and GEM.The apoptosis rates in MKN-74 cells in TSA+OXA group,5-Aza-CdR+OXA group and 5-Aza-CdR+TSA+OXA group were 28.067%,25.333% and 38.367%,respectively(P

Objective:To explore stem cells phenotype of human epidermal growth factor receptor 2(HER2)-overexpressing breast cancer cells,MDA-MB-453 by isolation of CD44+CD24-/low and side population(SP).Analyze the relationship between SP cells and the gene amplification levels of the key proteins,?-catenin and Notch-1 in Wnt and Notch signal pathways.And study the correlation of SP cells and the gene amplification of HER2 and human epidermal growth factor receptor 3(HER3).Methods:CD44+CD24-/low and SP phenotype were studied in MDA-MB-453(HER2-overexpressing cell line)and MCF7(HER2 negative cell line)respectively by flow cytometry.The gene amplification of ?-catenin and Notch-1 in Wnt and Notch pathways were also studied by reverse transcriptase polymerase chain reaction(RT-PCR).The gene amplification levels of the proteins mentioned,HER2 and HER3 were roughly compared in MDA-MB-453 cells and their SP by RT-PCR.Results:There was no CD44+CD24-/low subpopulation in MDA-MB-453 cells,However,SP could be observed in MDA-MB-453.The gene amplification levels of ?-catenin and Notch-1 in Wnt and Notch pathways were both observed in MDA-MB-453 and MCF7.Their gene amplification levels were more evident in SP of MDA-MB-453 than in MDA-MB-453 cells.However,the similar gene amplification levels of HER2 and HER3 were observed.Conclusion:SP can be enriched in breast cancer stem cells of MDA-MB-453.Wnt and Notch pathways are activated in MDA-MB-453,which may be related to maintenance and activity of breast cancer stem cells of MDA-MB-453.

Objective:To observe whether murine bone marrow mesenchymal stem cells(MSCs)implantation improves the survival of hepatocellular carcinoma(HCC)-bearing mice,and to investigate whether MSCs can differentiate to hepatocytes in HCC microenvironment in a mouse model of orthotopic HCC and its effects on tumor cells.Methods:Murine bone morrow MSCs were obtained through adherent culture method combined with magnetic cell sorting CD45-,CD11b-cells,labeled with CFSE in vitro.Phenotypes of MSCs were analyzed by flow cytometry.A murine model of orthotopic HCC was induced by intrahepatic injection of 5?105 murine H22 hepatoma cells in a BALB/c mouse.In this experiment,twelve BALB/c mice were randomly divided into MSCs implantation and saline administration groups on the 7th day after establishment of orthotropic HCC.CFSE labeled MSCs were injected into tumor and/or normal liver tissue in MSCs implantation group.The life span of HCC-bearing mice was observed.After three weeks,albumin was determined by immunohistochemistry.The livers of HCC-bearing mice were observed by light microscopy.Results:In MSCs group,the mean survival time was 25 days(95%Confidence interval:22-28 d),while,in the control group,mean survival time was 21 days(95%CI:20-23 d).However,the statistical difference was not obvious between the two groups(P=0.0713).It showed that the CFSE labeled cells mainly localized in the border of tumor,also a few in the tumor bed,and expressed albumin.Interestingly,there was a larger area of necrosis in the tumor bed,compared with that in the controls.Conclusion:It can be concluded that MSCs not only could engraft in livers of carcinoma-bearing BALB/c mice,but also could differentiate to hepatocyte-like cell.At the same time,MSCs might induce tumor cells necrosis.Further mechanism need to be studied.

Objective:To study the effects of hepatitis B virus(HBV)infection in vitro on differentiation of mesenchymal stem cells(MSCs)to liver cells.Methods:MSCs were isolated from human bone marrow by density gradient centrifugation,and expanded by adherent culture.MSCs were cultured under liver-stimulating condition,and different composition of serum was added to the induced medium:Group A:5% fetal bovine serum(FBS);Group B:2.5% FBS+2.5% HBV-containing serum;Group C:2.5% FBS+2.5% serum from healthy volunteers;Group D:the undifferentiated MSCs cultured in LG-DMEM+10% FBS.The expressions of a variety of hepatic lineage markers were analyzed by immunocytochemistry and immunofluorescence.The functionality of differentiated cells was assessed by their ablility to store glycogen.After 2 weeks of exposure to HBV infected serum,HBV-specific protein was also detected by immunocytochemistry.Results:As a result of our hepatic induction,the expressions of albumin(ALB)and alpha-fetoprotein(AFP)by MSCs were observed by immunocytochemical and immunofluorescence techniques.Moreover,MSCs had acquired the ability of glycogen storage which was characteristic of liver cells.Compared with the control group,the proliferation of MSCs was inhibited greatly by the virus-containing serum.After 2 weeks of exposure to HBV infected serum,the surface antigen(HBsAg)was detected in some induced MSCs.However,after immunocytochemical stain for ALB and AFP,there was not much difference between the Group B and C.The ability of glycogen storage of two groups were almost the same.Using confocal microscopy,we found the co-expressions of ALB and HBsAg in the same differentiated cells.Conclusion:The bone marrow MSCs have the ability to trans-differentiate into functional hepatocyte-like cells,hence may serve as a cell source for tissue engineering and cell therapy of hepatic diseases.HBV infected serum could inhibit the proliferation of MSCs in culture,but it seemed that the hepatic differentiation of the cell was unsuppressed.

In the undergraduate teaching assessment,"eaching quality control" is the core content of their inspection.This paper explained the quality control of the content,analyzed the challenge of improving the teaching quality and put forward the idea of constructing the perfect teaching quality control system from five aspects such as raising awareness and unifying thinking,establishing a sound teaching system,strengthening the organizational functions and responsibilities,grasping the key points and implementing the whole process of the quality control.