Objective To evaluate the effects of application of MgCI2, ATP, and ATP-MgCl2 on the funcrions of liver, kidney, heart and lung in experimontelly ischemic injuried animals, and to study the therapeutic role of exogenous Mg2+ and energy on repairmat of this injuny ming. Freeze etching, Histochemistry, Atomic Absorhtion Spectrum (AAS) and combine LM and EM. The experiment showed that MgCl2 and ATP protectived functions damnify organic (P>0.01) ,ATP-MgCl2 protected effect it notable results (P<0.01 ). Cell membrance was complete, glycogen became enriched in the cytoplasm, the uniform-distributed protein granule on Pside of plasma membtance of cell was found. There were positive material in the membrances and similar to normal group, cell michondia calcium decreased. Conclusions The exogenous application of ATP-MgCl was beneficial to the critical patient.

The recombinant protein drug usually has a short half-life after intravenous (IV) or subcutaneous (SC) administration. The methods of prolonging the half-life of recombinant protein drug in common use are mainly based on three principles: 1 Amplifying the molecule weight of protein drug; 2 Making use of drug balance in the blood; 3 Reducing Immunogenicity. Three methods were focused on: Analog construction, PEGylation and Albumin fusion technology. The characteristics, half-life and immunogenicity problem of their products in the market and under development are summarized.

Objective To analyze the change of disease spectrum and the direct cause of death in Xi'an Children's Hospital emergency patients in the past 2 years.Methods The cases of death in 130 emergency cases from 2009 to 2010 were retrospectively analyzed.Results The main causes of death in 2009 were pneumonia,congenital heart disease,asphyxia,neonatal pulmonary hemorrhage and intracranial hemorrhage.Meanwhile,The main cause of death in 2010 were pneumonia,asphyxia,viral encephalitis,congenital heart disease and neonatal pulmonary hemorrhage,which significantly changed compared with 2009.Major direct causes of death were found:respiratory failure in 25 cases (27.5％),heart failure in 17 patients ( 18.7％ ),shock in 17patients ( 18.7％ ),asphyxia in 16 cases ( 17.5％ ),and hernia in 16 cases ( 17.5％ ),Conclusion In order to reduce the mortality rate of emergency patients,we should strengthen health care,actively manage pulmonary infection,systemic infection,and shock,control heart failure,and prevent aspiration.

Objective To evaluate the significance of ultrasonography in diagnosis and differentiation of morning glory syndrome. Methods Ultrasonography,CT and fundus fluorescein angiography(FFA) features were retrospectively analyzed in 6 cases of morning glory syndrome.Results Ultrasonography, CT and FFA characters of morning glory syndrome were as follow: on the B-type ultrasonogram, the dark area of vitreous cavity extended to the posterior pole and optic papilla, projecting to the basal part of muscle cones, thus the posterior part of vitreous cavity looked like a converted bottleneck. Sometimes the light ribbon of retinal detachment can also be seen. On the A-type ultrasonogram, both vitreous cavity and bottleneck showed no ultrasonic echo and acted as an equable baseline without any evident wave crest. The attachment spot of optic nerve of eye turned thin and vitreous body protruded to the posterior wall of eyeball with spherical shape on CT imaging. In the earlier period of FFA, hypofluorescence appeared on the optic disc; then ,the abnormal arteriae and vein around the optic papilla were diplayed clearly; In the later period, optic discs were dyed with fluorescein. Conclusions Ultrasonography, CT and FFA showed imageological features of morning glory syndrome from different aspects, which was helpful for differentiating similar diseases such as optic disc coloboma. Especially, ultrasonography was considered as a more safe and reliable imageological method than other imageological methods in precisely diagnosing and differentiating morning glory syndrome and superior to the traditional fundus check which gives no integrity.

Objective To study the chemical constituents from Clerodendrum inerme.Methods The constituents were isolated and repeatedly purified on silica gel column chromatography and their structures were elucidated by spectral analysis.Results Eight compounds: friedelin (Ⅰ), stigmasterol (Ⅱ), betulinic acid (Ⅲ), acacetin (Ⅳ), syringic acid (Ⅴ), p-methoxybenzoic acid (Ⅵ), apigenin (Ⅶ), and daucosterol (Ⅷ) were isolated from the aerial part of C.inerme. Conclusion Compounds Ⅱ, Ⅲ, Ⅴ, Ⅵ, and Ⅷ are isolated from C.inerme for the first time.

0.05),and the distribution of genotypes was in Hardy-Weinberg equilibrium.Data analysis showed Met326Ile variant did not impact on FIns,FPG,HOMA index,BMI and gene expression of PI3-K,though the expression of PI3-K gene was lower in type 2 diabetes than in control group.Conclusion The Met326Ile variant of the p85? regulatory subunit of PI3-K is likely to be functionally normal in type 2 diabetes and health people.

Objective: To analyze the clinicopathological features and prognosis of patients with T1micN0M0, T1aN 0M0 and T1bN0M0 breast cancer, and to evaluate the survival and independent prognostic factors. Methods: The clinicopathological data from 4 487 patients who could receive surgical operation between January 2002 and December 2005 were collected. The clinicopathological features, recurrence, metastasis and survival of 376 patients with T1micN0M0, T1aN 0M0 and T1bN0M0 breast cancer were retrospectively analyzed. Results: Three hundred and seventy-six eligible patients were identified. Of these 376 patients, 66 patients (17.6%) had T1mic breast cancer (pT≤0.1 cm), 122 patients (32.4%) had T1mic breast cancer (0.1 cm < pT≤0.5 cm), and 188 patients (50.0%) had T1b breast cancer (0.5 cm < pT≤1.0 cm). With the lager tumor size, the histology grade was higher, the positive rate of estrogen receptor was lower, and the incidence of adjuvant chemotherapy was higher (P < 0.05). Univariate analysis showed that the age, hormone receptor and HER-2 status were prognostic factors for 5-year disease-free survival. Multivariate analysis showed that the hormone receptor and HER-2 were prognostic factors for 5-year disease-free survival. The 5-year recurrence risk in the positive hormone receptor group was four times as high as that in the negative hormone receptor group (hazard ratio was 4.995, 95% confidence interval was 1.113-22.424). In negative hormone receptor group, there was no statistically significant difference in the risk of 5-year recurrence between patients with positive HER-2 and negative HER-2 (hazard ratio was 2.757, 95% confidence interval was 0.849-8.955). Conclusion: HR and HER-2 status were independent prognostic factors for 5-year DFS in T1micN0M0, T 1aN0M0 and T1bN0M 0 breast cancer. Hormone receptor positive disease with positive HER-2 status was associated with a high risk of cancer recurrence. Copyright© 2011 by TUMOR.

Thermogravimetry (TG), TG-MS, Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM)-energy dispersive spectrometer(EDS) were adopted to investigate the pyrolysis characteristics of calamina. According to the findings of the qualitative and quantitative studies on the changes in the content of relevant elements, the whole shape, the functional groups, and the volatile components of calamina before and after being pyrolyzed, the 200-360, 580-750 degrees C were two sensitive temperature ranges related to the changes in effective component during calamina processing. Thermal weight loss was observed for ZnCO3, Zn(OH)2 and ZnCO3-2Zn(OH)2-H2O under 200-360 degrees C and for CaCO3 under 580-750 degrees C. The results of studies on chemical reaction kinetics showed good linear relations. This experiment integrated relevant methods and theories of physical chemistry and science of traditional Chinese medicine processing, and interpretes calamina processing techniques and mechanism, in order to provide a good example for modem studies on other traditional Chinese medicine processing.
Drug Combinations ; Drug Compounding ; methods ; Ferric Compounds ; chemistry ; Kinetics ; Temperature ; Zinc Oxide ; chemistry

Antigens, CD34 ; metabolism ; Carcinoma, Renal Cell ; metabolism ; pathology ; Central Nervous System Neoplasms ; metabolism ; pathology ; surgery ; ultrastructure ; Diagnosis, Differential ; Ependymoma ; metabolism ; pathology ; Hemangioblastoma ; metabolism ; pathology ; surgery ; ultrastructure ; Humans ; Meningeal Neoplasms ; metabolism ; pathology ; Meningioma ; metabolism ; pathology ; Mutation ; Neoplasm Recurrence, Local ; Prognosis ; Receptor, Epidermal Growth Factor ; metabolism ; Vimentin ; metabolism ; von Hippel-Lindau Disease ; genetics ; metabolism ; pathology ; surgery

As a member of nuclear receptor superfamily, farnesoid X receptor (FXR) has been shown to regulate numerous metabolic pathways, which include playing an important role in bile acid metabolism, maintaining lipid and glucose homeostasis when FXR is activated. With the prevalence of the glucose and lipids disorder, FXR attracts increasing attention. It may be a potential target for the treatment of type 2 diabetes mellitus and lipid disorders.
Bile Acids and Salts ; Diabetes Mellitus, Type 2 ; Glucose ; metabolism ; Homeostasis ; Humans ; Lipid Metabolism ; Receptors, Cytoplasmic and Nuclear ; metabolism