Objective:To isolate and screen the active marine microorganisms from East China Sea and to identify the phenotypes of the isolated active strains.Methods: The samples were widely collected from East China Sea and the active strains were screened out through an anti-bacterial model and a cytotoxic model.The isolated strains were subjected to phenotypical study,salt-aggregation test and rDNA ITS sequencing.Neighbor-joining phylogenetic trees were drawn by comparing the results of rDNA ITS sequencing with sequences of described species in the BLAST server of the National Center for Biotechnology Information(NCBI).One of the strains,subsequently named XD20,was identified to species level.Results: XD20,a halophilic strain with a highest tolerable salt concentration of 10.0% and suitable concentration range of 2.5% to 7.5%,was collected and isolated on the site of C01(122?26′30″,30?43′48″) 30 meters under water.XD20 showed an inhibitory effect on the growth of Candida albicans and a cytotoxic activity toward SMMC7721 and HeLa cells.XD20 produced arthroconidia by mycelium break and the arthroconidia had similar size and morphology to those of Wallemia sebi.rDNA ITS sequencing showed that the sequence of XD20 had a higher similarity to those of Wallemia sebi and the differences were located in ITS1 and ITS2 areas,both belong to variable region.Conclusion: XD20,an active halophilic strain fungus,has been obtained from the sea for the first time,and has been identified to be Wallemia sebi(Fr.) Arx.

Objective To evaluate central pancreatectomy in pancreatic surgery. Methods The clinical data of 19 patients who received central pancreatectomy was retrospectively analyzed in the Provincial Hospital, Shandong University from Jan 2002 to Jun 2008. Results Among 19 patients, 9 patients were of islet cell tumor,4 patients were mucinous cystoadenoma,3 patients were solid pseudopapilloma, 2 patients were pancreatic carcinoid tumor, 1 patient was of cyst-solid pseudopapilloma. After resection all of them had pathological negative margin. Length of stay was ( 17. 7 ± 7. 9) days ( ranging from 10 to 40 days ). Incidence of pancreatic fistula after this pancreatectomy was 31.6%. The follow-up period was from 6 months to 6 years. All of the 19 patients were alive except 1 who was lost to follow-up after 1. 5 years. There was no recurrence nor new-onset diabetes mellitus. Conclusions Middle pancreatectomy was a proper operative approach for benign or low-malignant tumors located in the neck or the body of the pancreas. This pancreatectomy may preserve endocrine and exocrine function, and decrease the risk of postoperative new-onset diabetes mellitns.

BACKGROUND: The influencion the environment on a culture is expressed via four routes. (1) the nature of the substrate or phase on or in which the cells grow (2) the physicochemical and physiological constitution of the medium, (3) the constitution of the gas phase, and (4) the incubation temperature. Melanization is closely related to the constitution and amounts of amino acids in the medium. OBJECTIVE: The aim of this study is to investigate whether there are some differences of proliferation and melanization in cultured B,F, mouse melanoma cells according to different culture media. METHODS: We examined the color of cell pellet, cell morphology, electron microscopic findings, cell counts and melanin conlensin BgF mouse melanoma cells cultured in Dulbeccos modified Eagles medium(DMEM), F-10, MCDB 153, Minimal essential medium(MEM), and RPMI 1640, respectively. RESULTS: 1. The color of cell pellet., ringed from dark gray to light brown. The order of the darkness was DMEM, MEM, RPMI 1640, MCDB 153, and F-10 medium. 2. Most Bg, mouse melanoma cells had an epithelioid morphology, but a few cells in MCDB 153 medium showed dendrites. On the 4th day after culture, the cells in F-10 medium were larger than those in the other media. 3. In the electron microscopic. findings, BF, mouse melanoma cells in DMEM and MEM con tained numerous stage IV nelanosomes, however, those in RPMI 1640 and MCDB 153 medium contained a few, and those in F-10 medium did few. 4. The number of BF, mouse melanoma cells were 1.42 + 0.06 x 10", 1.42 + 0.12 x 10", l. 17 + 0.08 x 10, 0.73 0.06 x 10, 0.32 0.01 x 10, in RPMI 1640, DMEM, MEM, F 10, and MCDB 153 medium, respectively. 5. In the MTT assay, the order of the optical density of B,F, mouse melanoma cells in various media was as followings, DMEM, RPMI 1640, MEM, F-10, and MCDB 153. 6. Compared with the melanin contents of B;F, mouse melanoma cells in DMEM, they were 77.97% in MEM, 67.91% in RPMI 1640 and MCDB 153 medium, and 55.94% in F-10 medium. CONCLUSION: The phenotypic changes of BF, mouse melanoma cells were induced by various culture rnedia and were reversilvle. Since the phenotypes of cells can be changed by the culture media, researchers should choose the appropriate culture medium for the cells.
Amino Acids ; Animals ; Cell Count ; Constitution and Bylaws ; Culture Media ; Darkness ; Dendrites ; Eagles ; Melanins ; Melanoma* ; Mice* ; Phenotype

Objective: To analyze the validity of the lie scale in University Personality Inventory (UPI). Methods: Relationship between choice of lie scale and UPI score , mental health was analyzed by UPI questionnaire of 2958 freshers.Results: Average every student chosed 2.37 lie items. Relationship between UPI score and numbers of the choosed lie items showed negative correlation. Numbers of lie items that was chosen by First class students was fewer than that was chosen by second class students, and numbers of lie items that was chosen by second class students was fewer than that was chosen by third class students.Conclusion:UPI lie scale had lost validity to evaluate attitude of respondent, so UPI lie scale should be revised.

Objective: To isolate and screen the active marine microorganisms from East China Sea and to identify the phenotypes of the isolated active strains. Methods: The samples were widely collected from East China Sea and the active strains were screened out through an anti-bacterial model and a cytotoxic model. The isolated strains were subjected to phenotypical study, salt-aggregation test and rDNA ITS sequencing. Neighbor-joining phylogenetic trees were drawn by comparing the results of rDNA ITS sequencing with sequences of described species in the BLAST server of the National Center for Biotechnology Information (NCBI). One of the strains, subsequently named XD20, was identified to species level. Results: XD20, a halophilic strain with a highest tolerable salt concentration of 10.0% and suitable concentration range of 2.5% to 7.5%, was collected and isolated on the site of C01 (122°26′30″, 30°43′48″) 30 meters under water. XD20 showed an inhibitory effect on the growth of Candida albicans and a cytotoxic activity toward SMMC7721 and HeLa cells. XD20 produced arthroconidia by mycelium break and the arthroconidia had similar size and morphology to those of Wallemia sebi. rDNA ITS sequencing showed that the sequence of XD20 had a higher similarity to those of Wallemia sebi and the differences were located in ITS1 and ITS2 areas, both belong to variable region. Conclusion: XD20, an active halophilic strain fungus, has been obtained from the sea for the first time, and has been identified to be Wallemia sebi (Fr.) Arx.

Participating in the international conferences helps to get the latest progress in cer-tain areas. It provides important channels of communication and platform for cooperation. The article summarized the categories of international conferences, access to it, way to apply for it, how to apply for financial support, visa and passport, as wellas preparations before. The author's experience attend-ing the seventh Asia-Pacific conference neurochemistry(APSN) held in Singapore, may offer some help for reference.

Hepatitis C virus (HCV) non structure 3 (NS3) protein and hepatitis B virus (HBV) X protein expressing plasmids were constructed with the vector pcDNA3 1(-). The plasmids were transfected into HepG2 cells and the viral proteins expressed in HepG2 cells were identified using Western blotting methods. Then the two recombined plasmids were transfected into HepG2 cells and were cotransfected into HepG2 cells with reporter plasmid pCAT3 promoter. The activity of CAT enzyme was detected by a CAT ELISA assay kit, which reflected the transactivating function of two proteins on SV40 early promoter. The findings indicated that the expression of two viral proteins were successfully detected in soluble protein cell extracts of transiently transfected HepG2 cells. HCV NS3 protein transactivated the CAT enzyme expressed at a value 3 5 fold higher than the control, while HBX protein transactivated at a value 4 4 times. It arrived at 8 5 times when transfected with two plamids simultaneously. The activating effect was increased in relation to the amount of plasmids. It was suggested that the two kinds of viral proteins had a transactivating effect on SV40 early promoter, and they acted synergistically. These results might contribute to explaining the mechanisms of liver injury or tumorigenesis induced by HCV or/and HBV infection

Hepatitis C virus (HCV) non structure 3 (NS3) gene was amplified from plasmid pBRTM3011 by employing polymerase chain reaction (PCR), and the amplified product was cloned into pcDNA3 1(-) vector. Then the recombinant plasmid pcDNA3 1(-) NS3 was transfected into HepG2 cells and was cotransfected into HepG2 cells with reporter plasmid pCAT3 promoter, respectively. HCV NS3 protein expressed in HepG2 cells was detected by reverse transcription PCR (RT PCR) and Western blotting method. The activity of CAT was detected by a ELISA kit, which reflected the transactivating function of HCV NS3 protein. The results showed that HepG2 cells transfected with pcDNA3 1(-) NS3 could express HCV NS3 protein. The expression of CAT in HepG2 cells transfected with the pcDNA3 1(-) NS3 was 4 6 fold higher than that of control plasmid. It was suggested that the recombinant plasmid pcDNA3 1(-) NS3 could be expressed in mammalian cell line, and had transactivating effect on SV40 early promoter

Objective To investigate the effect of nicardipine on the time-response relationships of vecuronium and atracuriumMethods Forty ASA grade Ⅰ-Ⅱ patients undergoing elective surgical operations were randomly allocated to four groups A single dose of vecuronium was intravenously administered at twice ED 95(01mg?kg -1) in groupⅠand combined with nicardipine 20?g?kg -1 in group Ⅱ Atracurium was given at the dose of twice ED 95 (05mg?kg -1) in groupⅢ and combined with nicardipine in group Ⅳ Anaesthesia was maintained with 50% nitrous oxide in oxygen and fentanyl in all groups Neuromuscular function was monitored with Biometer accelographResults The onset time of vecuronium was shortened from 26 min (groupⅠ) to 20 min (groupⅡ) (P

Objective To explore the clinical value of depression and event related potentials P300 in children with narcolepsy(NRL).Methods Event related potentials P300 test and self-rating depressive scale(SDS)test were performed in 39 children with NRL and 38 normal healthy children were selected as controls.Denmark Keypoint 4 path myoelectricity induced potential equipment and short sound stimulus were applied to determine event related potentials P300 test.Participants were required to hit the key for target stimulus,and reaction time and percentage of hits were recorded automatically.Long P300 and(or) low P3 amplitude of wave exceeded control group 2.5 s were abnormal.Zung SDS was adopted to evaluate somatization disorder,psychomotor disturbance,depression obstruction factor value.SPSS 12.0 software and t test were done to analyze the data.Results The abnormal rate of P300 in children with NRL of children group was 82.1%(32/39 cases),the latency of N2(260.7?22.1)ms and P3(353.1?25.9) ms of event related potentials of children with NRL group were longer than those in control group(241.0?23.2)ms,(341.3?26.1)ms,and there was significant difference between two groups(t=2.77,4.82 Pa