Objective To detect the expression of DNA repair enzyme O6-methylguanine DNA methyl-transferase(MGMT),cell proliferation-related nuclear protein(Ki-67)and P53 protein in gliomas,and inves-tigate the relationship and clinical significance among them and gloma grade. Methods 61 cases of brain glio-ma specimes and 16 cases of internal decompression of brain trauma were used to detect the expression of MGMT,Ki-67 and P53 by using immunohistochemical SP method. Results MGMT protein expression (19. 67%∶0,χ2 =3. 729,P=0. 062),Ki-67 protein expression(39. 34% ∶0,χ2 =5. 722,P=0. 016)and P53 protein expression(27. 87% ∶0,χ2 =9. 146,P=0. 002)showed significant differences in gliomas com-pared to normal brain tissues,the expression of Ki-67 was significantly higher in high-grade gliomas(Ⅲ-Ⅳ) than that in low-grade gliomas(Ⅰ-Ⅱ)(14 ∶10,χ2 =11. 718,P =0. 001). No significant difference was found between the MGMT and P53. Conclusion MGMT protein can be used as a biomarker in gliomas detec-tion;Ki-67 has a positive correlation with tumor grade,and can be used as a reference indicator of pathological grade;P53 protein expression may be used as a potential target for the treatment of gliomas.

Objective To explore the character of the myelodysplastic syndrome(MDS) with autoimmune disorders (AID).Methods The clinical data of 60 MDS patients were reviewed.Results 5 of 60 MDS patients had AID ,of which 3 had Graves' disease.The commonest MDS was refractory anemia with ringed sideroblasts (RAS).Conclusion MDS might occur with Graves' disease.Humoral immunological abnormality might play an important role in MDS patients with autoimmune disorders and FAB types might not be associated with this.

Administration, Inhalation ; Animals ; Erythrocyte Count ; Erythrocytes ; drug effects ; Female ; Formaldehyde ; toxicity ; Hemoglobins ; analysis ; Male ; Mice

Objective To isolate and identify the methicillin-resistant Staphylococcus aureus (MRSA) strains carrying Panton-Valentine leukocidin genes (pvl+-MRSA) from clinical samples and to further understand their molecular characteristics and infections caused by them.Methods Drug susceptibility test was performed to detect the drug resistance in 259 MRSA strains.pvl+-MRSA strains were screened out from those MRSA strains using cefoxitin slip test and mecA gene detection by PCR.Multiple PCR and multilocus sequence typing (MLST) were used for SCCmec and ST typing.Pulsed-field gel electrophoresis (PFGE) and cluster analysis were used to understand the genetic and epidemic features of the pvl+-MRSA strains.Different types of infections and diseases caused by the pvl+-MRSA strains were analyzed.ResultsAmong the 259 MRSA strains, 51 pvl+-MRSA strains were identified (19.7%, 51/259), of which 29 and 22 strains were respectively isolated from patients with community-acquired and hospital-acquired infections.ST59-SCCmecⅢ (35.3%, 18/51) was the predominant type of the 51 pvl+-MRSA strains, followed by ST59-SCCmecⅣ(25.5%, 13/51).But no predominant clone among those strains was revealed by the result of PFGE.Children, young-and middle-aged patients (≤44 years old) had a significantly higher positive rate of pvl+-MRSA than patients aged ≥45 years (P<0.05).Skin and soft tissue infection (47.1%, 24/51) was the most common disease caused by the pvl+-MRSA strains (P<0.05), followed by pneumonia (17.6%, 9/51).The pvl+-MRSA strains showed lower resistance to levofloxacin, gentamycin and rifampicine (7.8%-21.6%).No moxifloxacin-, nitrofurantoin-or linezolid-resistant pvl+-MRSA strains were identified.Conclusion The rate of pvl+-MRSA infection is high in the local population.ST59-SCCmecⅢ and ST59-SCCmecⅣ are the predominant types of pvl+ MRSA strains.Children, young-and middle-aged persons are the susceptible population.Skin and soft tissue infection and pneumonia are the common diseases caused by pvl+-MRSA.

OSCE is a new method which has strong objectivity for the evaluation of clin-ical skills examination.According to the actual situation of our college,we chose medicine sta-tion of OSCE to assess the level of the clinical skills of the clinical graduates.

Objective To explore diagnosisand treatment of intestinal obstruction by foodballs in children. Methods The clinical datum of 21 cases of intestinal obstruction by foodballs in children were retrospectively ana- lyzed.Results While admission,only 2 cases could provide the history of having eaten foodballs wholly which could not be digested easily.The characteristics of clinical manifestation were intestinal obstruction completely or incom- pletely.All 21 cases had been treated conservatively first,only one case was successful and the other 20 cases had been cured by surgery afterwords.Conclusion There is no specific feature in clinical manifestations of intestinal ob- struction by foodballs in children,so it is difficult to make the diagnosis,but it must be considered suspicious especial- ly for those from rural area and with simple intestinal obstruction with reasons not known yet.If conservative therapy is failure in a short period,an operation should be done immediately.During operation,it is necessary to examine the whole digestive system thoroughly.

Objective To study correlation of the retinal nerve epithelium layer thickness measured with different optical coher-ence tomography (OCT) in vivo with histological measurement. Design Experimental study. Participants 15 rabbit eyes. Methods The retina measurement position of 15 rabbit eyes were marked by laser, and then were scanned by OSE-1800 OCT and Stratus OCT. Reti-nal nerve epithelium layer thickness was measured in retinal histological shdes of rabbit eyes. The results measured with three methods were compared and linear regression analyses were done with SPSS11.5 software. Results The average retinal nerve epithelium layer thickness measured with OSE-1800 OCT, Stratus OCT and histological method were 119.5±7.4, 118.0±5.6, and 116.3±8.8μm respec-tively(P=0.292). Retinal nerve epithelium layer thickness measured with both OCT instruments had the best correlation (r=0.914, P= 0.000), and the thickness measured with Stratus OCT and histological method had the better correlation (r=0.872, P=0.001), and the thickness measured with OSE-1800 OCT and histological method had the significant correlation (r=0.833, P=0.002). Conclusions The retinal nerve epithelium layer thickness measured with different OCTs in vivo correlate well with histomorphometry, and the measure-ment of both OCT instruments are accurate. (Ophthalmol CHN, 2009, 18: 239-242)

Objective To detect the frequency and tissue distribution pattern of inhibin subunits in normal endometrium and periph-eral blood, and investigate the precise action in endometrium. Methods Normal cndometrial tissues were obtained from 16 women in the proliferative phase with salpingo-infertility, 11 women in the early secretory phase and 17 women in the late secretory phase with cervical cancer from Jan to Dee 2006. Expression of INH subunits were detected by immunohistochcmistry. INH-B in the blood samples were detec-ted. None of patients took any steroid drugs before 6 months of giving the samples. Results Immunohistochemistry showed that INH sub-units expressed in normal endometrial tissues. The expression intensity of INH subunits gradually increased in different menstrual phases. The level of inhibin-B in proliferative phase was higher than that in late secretory phase. Conclusions The relationship of inhibin-B level in serum and expression of inhibin subunits in endometrium showed that inhibin could be involved in autocrine/paracrine signaling and contrib-ute to several aspects of endometrial maturation.

To analyze the epidemiological characteristics of human infection with avian influenza A(H7N9) virus from 2013 to 2016 in Xiaoshan,China,we collected the epidemiological data of human A(H7N9) cases as well as the results of environmental monitoring and analyzed it by case study,descriptive epidemiological method,and statistical analysis.Results showed that a total of 22 cases were confirmed,including 12 deaths.All the cases occurred in winter and spring,and most of them had been exposed to live poultry,and with the underlying condition of chronic diseases.Chi-square test showed that the ratio of cases with chronic diseases in death group was higher (P=0.01).The environmental monitoring result showed there were distinctive seasonal differences of positive rate with peaks in winter and spring and nadirs in autumn and summer.Positive rates for the samples from external environmental monitoring were consistent with the distribution of the case onset.Due to rise of environmental monitoring positive rate,we should be alert to the occurrence of new cases,especially in rural areas where live poultry fairs still exist and among people with occupational exposure.We should further improve ability to prevent and control this disease.

AIM: To explore the possibility that the transcription factor GATA family member is involved in the regulation of Tie2 gene expression in the tumor necrosis factor-?(TNF-?) mediated inflammatory response.METHODS: The mRNA expression of GATA family members GATA2 and GATA3 was determined in primary human aortic endothelial cells(HAECs) and primary human pulmonary artery endothelial cells(HPAECs) before and after TNF-? treatment by real-time quantitative PCR.The activation of Tie2 gene promoter by GATA transcription factor was detected by luciferase assay.The electrophoretic mobility shift assays(EMSAs) and supershift assay were performed for verifying whether Tie2 gene expression was activated by the binding of GATA factors to Tie2 promoter.RESULTS: TNF-? increased the expression of GATA2 in HAECs and HPAECs.The highly expressed GATA2 was bound to the sequence(T/A) GATA(A/G) of Tie2 promoter,activated Tie2 gene promoter and up-regulated Tie2 gene expression.CONCLUSION: GATA transcription factor family member GATA2 up-regulates the expression of Tie2 gene in TNF-? mediated inflammatory response.