Animals ; Cartilage ; Cell Proliferation ; Femur ; Humans ; Intercellular Signaling Peptides and Proteins ; Male ; Osteoblasts ; Osteoclasts ; Osteogenesis ; Paraffin ; Rats ; Transforming Growth Factor beta

MAGE (melanoma antigen gene) is a tumor specific shared antigen, presented by HLA class I molecules, which is recognized by cytotoxic T lymphocytes. MAGE proteins are expressed in malignant tumor cells, in contrast to no expression in normal or benign tissues except for testis and placenta. MAGE might be a potential target for immunotherapy of malignant tumors. However, its biological aspects associated with cell cycle are not yet described. The flow cytometry is a useful tool for objective and quantitative analyses of heterogenous tumor cell population. To understand the status of MAGE related to cell cycle and its relationship with p53 as the G1 checkpoint regulator, p21, and PCNA as a proliferative index, we investigated expression of MAGE-3 protein, mutant p53, p21, and PCNA by flow cytometry and immunohistochemical stain. In addition, double stains for MAGE-3/p53, p53/PCNA, and p53/p21 were analysed with bivariate flow cytometry. DNA histograms using MAGE-3/PI (DNA) and p53/PI (DNA) were also analysed. The cell line (PNUH- 12) used for this study originated from a hypopharyngeal squamous cell carcinoma, which has point mutation (exon 7, C-->G) of p53. The expression rate of MAGE-3 was 83%, PCNA 85%, and p53 81%. No expression for p21 was identified. MAGE-3 was expressed in cytoplasm, while both PCNA and p53 were expressed in nuclei of tumor cells. With bivariate analyses, coexpression rates of MAGE-3/p53 and p53/PCNA were 0.96 and 0.97, respectively. Both MAGE-3 and p53 showed constantly high level throughout the cell cycle. These results suggest that expression of MAGE-3 and mutant p53 is not dependent on the cell cycle. p21 seems to be inactivated.
Carcinoma, Squamous Cell* ; Cell Cycle ; Cell Line* ; Coloring Agents ; Cytoplasm ; DNA ; Flow Cytometry* ; Immunotherapy ; Mutant Proteins ; Placenta ; Point Mutation ; Proliferating Cell Nuclear Antigen* ; T-Lymphocytes, Cytotoxic ; Testis

No abstract available.
Humans ; Humerus* ; Neck* ; Shoulder Dislocation* ; Shoulder* ; Tears*

No abstract available.
Arthritis*

No abstract available.
Liver* ; Ultrasonography*

BACKGROUND: Tinea faciei is a superficial dermatophyte infection on non-bearded skin of face. It is often confused with other dermatoses. Tinea faciei accounts for 3~4% of cases of tinea corporis. There have been only a few reports on the clinical and mycological features of tinea faciei. OBJECTIVE: This study aims to evaluate the demographic, clinical, and mycological features of tinea faciei in Daegu, Korea. METHODS: The medical records of 56 cases with tinea faciei from the Daegu catholic university medical center and Catholic skin clinic from January 2013 to December 2014 were evaluated retrospectively. RESULTS: The incidence was highest at 6th decade age group. The most frequent occupation of patients was agro-livestock area. Among 56 cases, the lesions were round patches in 40 cases (71.5%), followed by ring-shaped patches in 8 cases (25.0%) and plaques in 2 cases (3.5%). The most commonly isolated organism was Trichophyton rubrum (79.2%). CONCLUSION: Tinea faciei could easily be misdiagnosed clinically due to its abnormal presentation. When facing erythematous patches on face, physician should be aware of tinea faciei and undergo careful history taking with mycological study.
Academic Medical Centers ; Arthrodermataceae ; Daegu* ; Humans ; Incidence ; Korea ; Medical Records ; Occupations ; Retrospective Studies ; Skin ; Skin Diseases ; Tinea* ; Trichophyton

Early and longterm prognosis depended on the extents of acute myocardial infarction and residual myocardial function. Many clinical methods have been evaluated to determine their ability to estimate the size of myocardial infarcts. But because of many disadvantages simplified QRS scoring system, used with standard 12-lead EKG, was developed, which is noninvasive, inexpensive and easily releatible. Therefore, the correlation between QRS and peak CK,killip classification were evaluated in 55 patients with initial acute myocardial infarction at the Chung-Ang University, Yong-San and Pil-Dong Hospital during the period from January 1983 to December, 1986. The results were as follows : 1) The average QRS score of the anterior wall infarction is 7.1 and that of the inferior wall infarction is 4.5 and there is a statistical significance(p<0.01). 2) In the acute myocardial infarction, the correlation coefficeient between the QRS score and the killip classification is 0.66(p<0.05). 3) In the acute myocardial infarction, the average QRS score is 6.3 and the correlation coefficient between the leak CK and the QRS score is 0.52(p<0.05). 4) In the anterior wall infarction, the average QRS score is 7.1 and the correlation coefficient between the peak Ck and QRS score is 0.48(p<0.05). 5) In the inferior wall infarction, the average QRS score is a 4.5 and the correlation coefficient between the leak CK and QRS score is 0.79(p<0.05).
Classification ; Electrocardiography ; Humans ; Infarction ; Myocardial Infarction* ; Prognosis

BACKGROUND: Rosacea is a common skin condition, but the currently available treatments are not satisfactory. OBJECTIVE: We wanted to assess the efficacy and safety of photopneumatic therapy (PPx), which delivers pneumatic energy and broadband light (400~1,200 nm), for the treatment of erythematotelangiectatic rosacea. METHODS: Twenty two patients with erythematotelangiectatic rosacea were treated 3~5 times with PPx. Three independent dermatologists evaluated the efficacy of PPx by comparing the photographs taken before and after treatment. Patient self-assessment was also done by interviews. RESULTS: The photographic assessment by the dermatologists of the improvement showed that >50% improvement occurred in 64% (14/22) of the patients. The specific categories of improvement were as follows: poor (<25% improvement), 1 patient; fair (25~50% improvement), 7 patients; good (50~75% improvement), 11 patients; and excellent (>75% improvement), 3 patients. Based on the patients' self-assessment, 20 patients (91%) acknowledged an improvement. CONCLUSION: PPx is an effective and safe treatment option for erythematotelangiectatic rosacea.
Humans ; Light ; Rosacea ; Self-Assessment ; Skin

Hypoglycemic hemiplegia is an under-recognized manifestation of hypoglycemia and mimics cerebrovascular dis-ease, especially in elderly patients. We report a 70-year-old diabetic woman with hypoglycemic hemiplegia, whose dif-fusion- and perfusion- weighted images showed large cortical high signal intensities and hypoperfusion of the right hemisphere during the period of hypoglycemia. We suggest that the regional cerebral blood redistribution and selective vulnerability in hypoglycemia are responsible for the laterality of neurological deficits observed during hypoglycemic episodes.
Aged ; Female ; Hemiplegia* ; Humans ; Hypoglycemia ; Magnetic Resonance Imaging*

No abstract available.
Diabetes Mellitus, Type 2* ; Humans