MicroRNAs (miRNAs)can influence the proliferation,apoptosis,invasion and metastasis of esophageal squamous cell carcinoma (ESCC)cells by signal transduction,epithelial-mesenchymal transi-tion,angiogenesis and other regulating mechanisms.Specific serum miRNAs can serve as novel diagnostic and prognostic biomarkers of ESCC.Recent studies have indicated that miRNAs can improve the radiation sensitivi-ty and even reverse multi-drug resistance of ESCC,holding great clinical value.

35) and the third strategy of providing diagnosis to those tested positive at screenings in mid-term pregnancy could, respectively, lead to the diagnosis of 0.93 and 1.47 cases of DS per 10 thousand pregnant women, but they could also induce, respectively, 1.58 and 0.28 cases of miscarriage of normal fetuses due to diagnosis procedures. The costs of one DS diagnosis were, respectively, 108890.98 yuan and 443683.11 yuan. Conclusion Taking into consideration both cost-effectiveness and safety factors, priority ought to be given to the third strategy.

Medical students' English autonomy learning is different from other students,basing on some relevant autonomy learning theories,the authors investigated such aspects as learning motivation,achievement,length of time-spending,choice of learning strategy,major,gender,learning phase and family background of medical students'English autonomy learning.And at the same time,the authors analyzed its current situation and put forward some solutions to the existing problems.

Trypsin was purified from crude material of pig pancreas by AOT/isooctane reversed micellar system.The influence of the main operating parameters such as ethanol concentration in forward and backward extraction,pH,KCl and AOT concentration,temperature were investigated.Forward and backward extraction recovery of trypsin reached almost 90% and neared 100%,respectively.Finally,about 88% of total yield was obtained,and the specific activity of trypsin was increased to over 1800U/mg with purification factor of 5 folds more.In AOT-isooctane reverse micellar extraction system,denaturation or precipitation of proteins always occured due to strong electrostatic interaction between AOT-proteins molecules.It had been resolved by adding ethanol into reverse micellar system,and no denaturation was observed.Otherwise,the phase separation time was shortened significantly because of ethanol added.It was only 10 minutes or less to reach phases separation after forward and backward extraction.If this method can be applied in industry,efficiency will be greatly improved.

Background and purpose:The skeleton is the most common site of tumor metastasis in lung cancer.Skeletal complications from bone metastasis,such as severe bone pain,functional impairment,may substantially reduce quality of life,in some situations,may result in death.We explored the efficacy of ibandronate combined with chemotherapy in treatment of lung cancer with bone metastasis.Methods:Sixty-four lung cancer patients with bone metastasis were randomized into two groups:38 patients in study group received normal chemotherapy combined with ibandronate,and 26 patients in control group received normal chemotherapy only.Results:The response rate of pain relief in the study group and control group was 71.1% and 42.3%,respectively(P=0.006).In study group,serum alkaline phosphatise and serum calcium was decreased after treatment by ibandronate combined with chemotherapy(P0.05).Conclusion:Ibandronate is safe and well tolerated. Ibandronate combined with chemotherapy is effective in relief of the pain caused by bone metastases and may inhibit bone metastasis.

Objective To investigate the peripheral blood Th17 and CD4 + CD25 + regulatory T cell levels and their correlations in patients with primary hepatocellular carcinoma (PHC).Methods Peripheral blood samples were collected from 30 PHC patients and 25 healthy controls in the First Affiliated Hospital of Zhejiang University from June 2008 to May 2009.Mononuclear cells were isolated and the Th17 and CD4 + CD25 + regulatory T cells were detected by flow cytometry and compared between patients and controls by t test.Spearman test was performed to analyze the correlation of Th17 with CD4 + CD25 +regulatory T cell concentrations.Results The levels of Th17 and CD4 + CD25 + regulatory T cells in peripheral blood in healthy controls were (2.10 ± 0.87) ％ and (7.10 ± 2.32) ％ ; while those in PHC patients were (3.38±1.68)％ and (11.78±5.62)％ (t=3.640 and 4.162,P＜0.01).The level of Th17 cells was positively associated with that of CD4 + CD25 + regulatory T cells in PHC patients (r =0.821,P ＜0.01).Conclusion The levels of Th17 and CD4 + CD25 + regulatory T cells in peripheral blood are high in PHC patients and positively correlated with each other,which indicates that CD4 + CD25 + regulatory T cells may contribute to the disease progression and pathogenesis of carcinoma through inducing Th17 cells differentiation.

Objective To investigate the prevalence of hepatitis B virus infection and its epidemiological characteristics among planned pregnancy couples in Chongqing.Methods A total of 10 712 planned pregnancy couples were selected by proportionally stratified multi-stage random sampling method during April and December 2013.A questionnaire survey was performed, and enzyme linked immunosorbent assay ( ELISA) was used for hepatitis B virus surface antigen ( HBsAg ) and hepatitis B surface antibody ( anti-HBs) detection.SAS 8.2 was adopted for data process.Chi-square test and test for linear trend were performed to compare the rates of HBsAg and anti-HBs among different populations.Results Among 21 424 individuals, 1 718 (8.02%) were HBsAg positive.The positive rate of HBsAg in husbands was 9.48%, which was significantly higher than that in wives (6.55%,χ2 =63.39, P<0.01).Anti-HBs was positive in 8 967 (41.85%) individuals, and the positive rates in husbands and wives were 42.39% and 41.32%respectively (χ2 =2.54, P>0.05).The positive rates of HBsAg and anti-HBs increased with the age (χ2 =2.98 and 9.83,P<0.01).The highest HBsAg positive rate and lowest anti-HBs positive rate were observed among individuals of southeast of Chongqing.The positive rates of HBsAg and anti-HBs varied in people with different occupation.The highest HBsAg and anti-HBs positive rates were found in private business owners (9.18%) and public employees (49.69%) , respectively;and the lowest HBsAg and anti-HBs positive rates were found in public employees (6.79%) and farmers (39.08%), respectively.As the education level increased, HBsAg positive rate was decreasing (χ2 =-3.33, P<0.01), and anti-HBs positive rate was increasing (χ2 =9.73, P<0.01).Conclusions The prevalence of HBV infection is still high among planned pregnancy couples in Chongqing in the year of 2013.Vaccination and the related treatments should be enforced in population with high risk of HIV infection.

Objective To explore the effects of the pre-pregnant body mass index(BMI) and the pregnancy growth on the mater-nal and neonatal outcomes .Methods 250 pregnant women with the built file ,regular antenatal inspection and hospital delivery in our hospital during 2011 were selected and divided into the emaciation group ,the appropriate group and the obesity group according to the pre-pregnant BMI .The occurrence rates of cesarean section ,fetal macrosomia ,low birth weight ,postpartum hemorrhage ,ges-tational diabetes ,gestational hypertension disease ,fetal distress ,neonatal asphyxia and puerperal infection were compared among the various groups .In addition ,according to the different growth amplitudes of pregnancy BMI ,the pregnant women were re-divided in-to 3 groups(weight gain insufficient group ,weight gain appropriate group and weight gain overmuch group ) and the different ma-ternal and neonatal outcomes were compared .Results The occurrence rates of cesarean section ,macrosomia ,gestational diabetes and gestational hypertension disease in the obesity group were significantly higher than those in the emaciation group and the appro-priate group(P<0 .05) ,while the occurrence rate of the low birth weight in the emaciation group was significantly higher than that of the appropriate group and the obesity group(P<0 .05);the occurrence rates of cesarean section ,macrosomia ,gestational diabetes and gestational hypertension disease in the weight gain overmuch group were significantly higher than those in the weight gain in-sufficient group and the weight gain appropriate group(P<0 .05) ,while the occurrence rate of low birth weight in the weight gain insufficient group was significantly higher than that of the weight gain appropriate group and the weight gain overmuch group (P<0 .05) .Conclusion The maternal and neonatal outcomes in the pregnant women with pre-pregnant BMI exceeding the standard and pregnant BMI overmuch growth are very worrying .The growth of pre-pregnant BMI and pregnant BMI is one of the important indi-cators for monitoring the maternal and neonatal complications .

0.05).There was a positive correlation between the tempo of the engraftment and the progressive increase of the doses of MNC in the range of (3～5.99)?108/kg,but which was not found in the range of 6?108/kg.Conclusion These results indicate that MNC taken as an index for the measurement of the hematopoietic stem cell/progenitor content can reliably predict hematopoietic reconstitution after both HLA-matched and-mismatched sibling donor peripheral blood stem cell transplantation,with the rate and tempo of engraftment comparable with that in CD34+ cell group,and however,the target dose was reached with one apheresis in all 83 donors in MNC group(100%),as compared with 14 donors in CD34+ cell group(37.84%),suggesting that MNC may replace CD34+ cell as an independent index for the assessment of the stem cell/progenitor content in clinical practice.