Objective To explore the pathologic changes of the opening blood-brain barrier(BBB) in the early stages of 50% TBSA scalded rabbits.Methods Ten male rabbits inflicted with 50% TBSA of Ⅲ degree were randomized into five groups: control,postburn 1 h,2 h,3 h,4 h.Brain tissues were isolated at different phases and observed in gross,by microscope and transmission electron microscope.Results Endothelial cells swelled and BBB began to open in 2 h after scald.The tight junction of endothelial cells became vague and the opening of BBB increased as lesion time prolonged.Conclusion As the lesion time prolonged,the injury of endothelial cells became more serious and the opening of BBB progressed,which resulted in the formation of brain edema.

To investigate the features of CT, ultrasonography and fundus fluorescein angiography (FFA) of morning glory syndrome, the data on CT, A/B-scan ultrasonography and fundus fluorescein angiography (FFA) were retrospectively analyzed in 8 cases of morning glory syndrome (MGS). Among those cases, 6 were examined with CT, 4 with FFA and 8 with A/B-scan ultrasonography. Results showed that the characteristics of CT, A/B-scan ultrasonography and FFA in MGS included: (1) The attachment spot of optic nerve became thin and vitreous body protruded to the posterior wall of eyeball with a spherical shape on CT image; (2) in the early period of FFA, hypofluorescence appeared on the optic, the abnormal arteriae and veins around the optic papilla were displayed clearly and in the late period, optic disc was stained with fluorescein; (3) on B-scan ultrasonogram, the vitreous cavity extended to the posterior pole and optic papilla, and projected to the basal part of muscle cones and thus the posterior part of vitreous cavity looked like an upside-down bottleneck. Sometimes the echogenic band of retinal detachment could also be seen. On A-scan ultrasonogram, both vitreous cavity and bottleneck showed no ultrasonic echoes and presented a base line without any evident wave crest. It is concluded that CT, A /B-scan ultrasonography and FFA could show the imageological features of MGS from different aspects, which helps clinicians to differentiate it from other diseases such as optic disc coloboma. CT and A /B-scan ultrasonography, in particular, are considered to be reliable imageological methods for the accurate diagnosis of MGS and are superior to the traditional techniques.

In recent years, there is increasing evidence that the pathogenesis of Parkinson's disease (PD) may involve the more generation of reactive oxygen species, and investigations on patients have shown that PD is under a status of oxidative stress. The defense against the toxic effects of reactive oxygen species is an essential task within the brain. An important component of the cellular detoxification of reactive oxygen species is the antioxidant glutathione. Consequently, it seems reasonable to propose that increase of brain concentrations of glutathione including glutathione analogs or precursors could be very effective in diminishing the cumulative effects of oxidative damage, and have been investigated as potential therapeutic targets in the treatment and prevention of PD or other neurodegenerative disease.

Many countries have developed standard strategy under the tendency of the economic globalization.TCM international standardization faces more and more competitions.This article analyzes the competitors and competitive status of TCM international standardization by the research methods of competitive intelligence,competitor analysis and SWOT analysis,and puts forward corresponding strategic measures in details.

Objective To study the effect of femoral offset reconstruction ON the Harris hip score and the hip ROM (range of motion) after artificial hip replacement in femoral neck fractures. Methods Follow-ups were conducted for 47 patients who had undergone artificial hip replacements for their unilateral femoral neck fractures. We took X-ray pictures to compare the femoral offsets at the affected and unaffected hips. The operated hips were evaluated by the Harris hip score. Statistical analyses were performed with SPSS l2.0 software. Results The Harris hip score of the operated hip was positively related to femoral offset reconstruction(t=7.25,P＜0.001).There was a significant and positive correlation between femoral offset and the hip abductor ROM(r=0.80,P＜0.001).The rate of femoral offset reconstruction had no significant difference between total hip arthroplasty and bipolar hip arthroplasty (χ2=0,P＞0.05).Conclusions Reconstruction of femoral offset after artificial hip replacement in the femoral neck fractures can improve funetional recovery and ROM of the hip. Femoral offset reconstruction is uncorrelated to the selection of total hip arthroplasty or bipolar hip arthroplasty.

Objective Discussing the rationality of psychological intervening therapy in the treatment of early breast neoplasm. Methods By summarizing and reviewing 84 patients suffering from the early breast cancer between January of 2003 and December of 2005, we procured some information of their clinical manifes-tations, clinical therapy and statistic results of psychological assessment. Results By intervening the psycho-logical therapy, the patients' self-efficiency and living quality can be bettered. Conclusion Patients with early breast neoplasm need to have a comprehensive treatment with general or local therapy and individual psychologi-cal intervention. Nevertheless, it is rather practical to enhance the general level of prevention and therapy of breast neoplasm through the research of psychological problems after the operation.

Objective To prepare the specific monoclonal antibodies(mAb) of Mycoplasma pneumonia(MP) and to establish a colloidal gold rapid detection method of MP by using mAb .Methods The nasopharyngeal swab samples were collected from chil‐dren patients with acute respiratory tract infection ,separated and cultured and performed the MP identification ,MP antigen was pu‐rified ,mAb of MP was prepared ,then the MP colloidal gold test strip was established by using MP mAb .The throat swab sample was collected from the patients with suspected MP infection and detected by using the fluorescent quantitative PCR and colloidal gold test strip .The detection results were observed and statistically analyzed .Results MP strain was successfully isolated and inac‐tivated ,the MP antigen was purified .The mouse was immunized by using this antigen ,19 strians of mAb against MP were prepared by using the hybridoma technique .Among them ,2 strains of mAb with high titer and good specificity(MP‐5 and MP‐19) were se‐lected as the raw materials for preparing the MP colloidal gold rapid test strip .The lowest detection limit was 20 ng .The clinical samples were detected by using the MP colloidal gold rapid test strip and fluorescent quantitative PCR .The results showed that the sensitivity of MP colloidal gold rapid test strip by using this established method was 88 .2% and its specificity was 82 .6% .Conclu‐sion specific mAbs against MP is prepared and the colloidal gold rapid detection method is preliminarily established which provides the help for rapid diagnosis in the patients with MP infection .

Advanced hospitals and high-level hospitals are different from grass-root hospitals,since they not only treat difficult diseases but also have the obligation of scientific studies and teaching.Grass-root hospitals mainly focus on treating common diseases,and only conduct a small amount of teaching,thus resulting in the difference between grass-root hospitals and large-scale hospitals in the aspect of purchasing medical equipment.Because the money for grass-root hospitals may only be used as designated and some even comes from loan or collecting,we have to pinch pennies and make the best use of everything so as to recover the cost rapidly and bring the equipment into full play.

When a series of omics technologies such as genomics, epigenomics and proteomics are proposed, the concept of metabolomics occurs. Metabolomics is a top-down system biology approach, which analyzes endogenous metabolites by using high throughput, high resolution and high sensitivity based on metabolic analysis platform. By identifying characteristic biomarkers and analyzing the biomarkers of metabolic pathway, it will provide a new way for the diagnosis and treatment of diseases. Therefore, it is more and more widely used in bronchial asthma, chronic obstructive pulmonary disease, pulmonary cystic fibrosis, acute respiratory distress syndrome, pulmonary tuberculosis, lung cancer and other respiratory diseases. In this paper, the application of metabolome analysis in respiratory disease of recent years has been briefly reviewed.