Objective To discuss the rational use of antibiotic sequential therapy in the elderly hospitalized with acute lower respiratory tract infection.Methods Using a clinical prospective intervention study of random sample contrast and minimum cost analysis of pharmacoeconomic compared the clinical curative effect of two kinds of therapy methods including intravenous-oral sequential therapy group(sequential group)and continuous intravenous infusion therapy group(intravenous group).Meanwhile,the differences of two groups in the bacterial clearance rate,treatment time of intravenous infusion and length of hospitalization day were also investigated. Furthermore the costs of antibiotics of two groups were evaluated as well in the pharmacoeconomic. Results Compared with two therapies,it was shown equivalence in bacteriology and clinical outcome. Pharmacoedynamics of them were nearly same. There was no significant difference(P＞0.05).In the clinical curative effect,two therapies' bacteria clearance,the times of restoring to normal of temperature,blood routine examination and chest X-ray were practically closed to each other,difference was in significant(P＞0.05).However,the time of intravenous infusion therapy time and the length of hospitalization day had notable difference,and antibacterial cost of sequential group Was obviously lower than those of intravenous group,there were significant difference(P＜0.05).Conclusion Sequential therapy was safe,operative,and had more reasonable cost-effect ratio in treating the elderly with acute lower respiratory tract infection. Moreover the optimum time from intravenous infusion therapy switching to oral antibiotics therapy was about 7 days.

AIM:To study the sedation, diuresis and anticoagulation effects of Ji-Ming-San (JMS) in animal models for exploring the circadian variations of the dosing action and verifying the rationality of administering JMS on special time. METHODS: The sedation effect and circadian difference were determined by observing the time session of ambulation, raising double-forefoot test and autonomic activity of model mice. The diuretic effect and the circadian variation in rats were examined by metabolic cage test while the anticoagulation and the circadian change were observed by glass test. RESULTS: JMS produced significant sedation effect by reducing time session of ambulation, raising upper limbs frequencies/time and autonomic activity and the built-in rhythm with autonomic activity disappeared after JMS administration. JMS had satisfactory diuretic effect and total urinary output in water-loaded rats was increased after dosing. The diuretic effect of dosing showed a circadian rhythm with more significant output during the night than that during the daytime. Also, JMS prolonged the clotting time significantly and the action exhibited circadian difference. As compared with administration at the night, the clotting time was more prolonged at the daytime. CONCLUSION: JMS can produce obvious sedation, diuresis and anticoagulation effects with varied circadian rhythm. The findings suggest that the effect of administering JMS is better at the end of rest phase than other time session.

Objective To explore the establishment methods of animal models of intestinal multidrug-resistant bacterial (Pseudomonas aeruginosa, PA) infection, and to provide a good model for experimental research and treatment for infection caused by drug-resistant bacteria .Methods Male BALB/c mice were divided into four groups:Normal con-trol group, MDR-PA group, MDR-PA +antibiotics group, and MDR-PA +fasting group.They were given saline, PA suspension, PA suspension after free drinking with ceftriaxone water and PA suspension after fasting , respectively , by ga-vage.Colon tissues were collected for pathological examination and inflammation scoring .The expressions of TNF-αand INF-γin the colon tissue was assessed at the end of the experiment .Results Pathology showed different degrees of inflam-matory changes in the colon tissues .Compared with the normal control group , the expressions of TNF-αand INF-γhad sig-nificant differences with that of experimental groups .Conclusions All the three methods used in this experiment can be successfully used to establish mouse models with intestinal infection caused by multidrug-resistant Pseudomonas aeruginosa, and may be chosen for experimental studies according to different research purposes .

Objective To optimize the fermentation conditions of molluscicidal endophyte LL3026 from Buddleia lindleyana. Method The medium composition and cultivation conditions were optimized by orthogonal and single factor experiments. Re-sults The experiments showed that the conditions of initial pH 3,fermentation temperature 30℃,volume of liquid 100 ml(250 ml Erlenmeyer flask),and 3D-xylitol 0.5 g/L were optimum,and the molluscicidal activity of the fermentation filtrate reached 95%. After three hatches of cultivation,the predicted values were verified by validation experiments. Conclusion Endophyte LL3026 from Buddleia lindleyana has a good molluscicidal activity after the optimization.

Objective To prepare drug carrier non-PEG blank liposomes, and study its properties.Methods High purity of egg yolk lecithin and cholesterol were used as film forming material.The high pressure homogeneous method-extrusion method and high pressure homogeneous method-ultrasonic method were used to prepare non-PEG blank liposomes.After that how the method of high pressure homogeneous, extrusion and ultrasonic influence the particle size of blank liposomes were studied, and the physical stability of blank liposomes were investigated.ResuIts The particle size of blank liposomes prepared by high pressure homogeneous method-extrusion method was about 86 nm, and its polydispersity index was 0.170.While the particle size of the blank liposomes prepared by high pressure homogeneous method-ultrasonic method was about 91 nm, and its polydispersity index was 0.362.ConcIusion Compared with high pressure homogeneous method-ultrasonic method, the blank liposomes prepared by high pressure homogeneous method-extrusion method had some advantanges, such as smaller particle size, narrow particle size distribution and high stability.

Objective To investigate the adverse reactions influence factors of deep sedation anesthesia.Methods 120 cases with elective gastrointestinal tumor resection were chosen and given deep sedation anesthesia.The occurrence of intranperative bemodynamic changes at each time point and adverse reaction were statistically analyzed.Results SBP,DBP,HR and SpO2 values at different anesthesia time point had significant differences (F =9.688,10.258,7.586,8.992,all P =0.000).26 cases had intraoperative and postoperative adverse reactions,the incidence was 21.7％,8 cases with hypotension,4 cases with bradycardia,6 cases with nausea and vomiting,2 cases with apnea,6 cases with other adverse reactions.No death and serious adverse reactions occurred.Senility,depth anesthesia application history,psychological stress and obesity were risk factors of intraoperative and postoperative adverse reactions of gastrointestinal tumor by deep sedation anesthesia(OR values =9.806,0.119,0.105,10.521,all P ＜0.05).Conclusion Adverse reaction incidence of gastrointestinal tumor by deep sedation anesthesia was high,and more performance for hemodynamic response,anesthesiology doctors could improve patients prognosis by regulating the perioperative risk factors.

Liver is one of the most predilection sites of hematogenous metastasis of a variety of malignances,especially for gastrointestinal tumors.Surgical resection was the first choice for the treatment of liver metastases,while it could not apply to patients who had multiple metastatic lesions or other organs involvement.Interventional technique has been widely recognized for the advantages of minimal trauma,little pain,quick recovery and obvious efficacy.

Objective To evaluate the efficacy and safety of COOK cervical dilatation balloon for induced labor in full -term pregnancy . Methods There were 98 cases of cervical ripening and induced labor by using COOK balloon from January 2014 to April 2015 ( experimental group ) and 130 cases of cervical ripening and induced labor by using oxytocin from January 2010 to September 2013 ( control group ) .The cervical scores , childbirth way , and adverse reactions were compared between the two groups . Results After the removal of the balloon, the cervical scores in the experimental group increased from (2.38 ±0.70) points to (6.15 ±0.96) points (t=31.412, P =0.000).The rate of vaginal delivery in the experimental group (79.6%, 78/98) was significantly higher than that in the control group [62.3%(81/130),χ2 =7.910, P=0.005].The incidence of chorioamnionitis in the control group was 5.4% (7/130), which was significantly lower than that in the experimental group [18.4% (18/98), χ2 =9.647,P=0.002).The other adverse reactions in two groups had no statistical difference (P >0.05). Conclusions Use of COOK cervical dilation balloon is a safe and effective method to promote cervical ripening .In combination with artificial rupturing membrane or intravenous infusion of oxytocin can improve the success rate of full -term pregnancy induced labor .

Objective To investigate the changes of myoepithelial cells in mammary atypical hyperplasia and breast cancer. Methods SP immunohistochemistry was used to detect actin expression in normal breast tissue, grade Ⅰ, Ⅱ and Ⅲ atypical hyperplasia and breast cancer. Electromicroscopy was used to observe the changes of ultrastructure of myoepithelial cells. Results Actin was only detected in myoepithelial cells of normal breast tissue and grade Ⅰand Ⅱ atypical hyperplasia. The positive expression rates of actin in grade Ⅲ atypical hyperplasia(70%) and breast cancer(90%) were significantly higher than that in grade Ⅱ atypical hyperplasia(10%), P

AIM:To observe effects of Jiming Powder(Semen arecae,Pericarpium citri reticulatae,Fructus Chaenomelis,Fructus Evodiae,Folium Perillae,Radix Platycodonis,and Rhizoma Zingiberis recens) on anticoagulation and hemorheology and on the circadian variations in order to verify the rational practice of timing administering the drug by our ancestors. METHODS:The effect of Jiming Powder on clotting time(CT) was observed by using glass-tube method in mice. Quick test was used to determine the prothrombin time(PT) and circadian variations in rats. The experimental animal models of blood stasis syndrome were prepared by subcutaneous injection of adrenaline in combination with ice water bath for observing the effect of Jiming Powder on hemorheology in such types of animals,and the maximum rate of platelet aggregation was determined by method of Born turbidimetry. RESULTS:Jiming Powder could significantly delay CT and PT characteristic of circadian rhythm. As compared with the administration at daytime,CT and PT were greatly prolonged during the night. Besides,the hemorheological parameters were improved markedly in blood,plasma and fibrinogen viscosity,which suggested that the drug administration was more effective during the night than daytime. Also,the findings showed that Jiming Powder could prevent platelet aggregation induced by adenosine diphosphate. CONCLUSION:Jiming Powder plays a role in anticoagulation and improves the indication of hemorhelogy in the blood-stasis rats model,showing dosing time-dependent.