The modernization strategy of traditional Chinese medicine (TCM) has been implemented for 20 years, which has provided a strong impetus to the development of TCM and gained remarkable achievements in scientific research platform, research result, industry scale, clinical evaluation, internationalization and professionals training as well. The achievements of TCM modernization greatly improved the clinical service ability and level of TCM, broadened the scope of service, become the important foundation of the big health industry. TCM has played an irreplaceable role in the health care reform, benefiting people's livelihood, promoting industrial structure adjustment and cultivating strategic emerging industries. This article summarized the main achievements of the modernization of TCM and prospected the direction and tasks in the next 20 years in order to further promote the modernization process of TCM.
Biomedical Research ; history ; standards ; trends ; China ; History, 20th Century ; History, 21st Century ; Humans ; Medicine, Chinese Traditional ; history ; standards ; trends ; Social Change ; history

Objective To observe the effects of aerobic training on plasma tissue plasminogen activator/plasminogen activator inhibitor-1 and postaglandin I2/thromboxanes A2 in rats fed a high methionine diet. Methods Ninetysix male Wistar rats were randomly divided into a normal diet control group (CR), a high methionine diet group (MR)and a high methionine diet plus 90 min aerobic training intervention group (T + MR). After 8 weeks of training, the plasma level of homocysteine (Hcy) 6-keto-PGF1α, TXB2, t-PA and PAI-1 were measured. Results Plasma Hcy in the MR group increased two-fold as compared with the CR group, whereas t-PA/PAI-1 and 6-keto-PGF1α/TXB2 of the MR group decreased significantly. Plasma Hcy in the T + MR group significantly decreased, while plasma t-PA/PAI-1 and 6-ketoPGF1α/TXB2 increased significantly compared with the MR group. The above parameters were not different in the T + MR group from those of the CR group. Conclusions Hyperhomocysteinemia could be induced by a high methionine diet.Proper training can decrease the plasma Hcy level of rats fed with a high methionine diet, and correcting the imbalance of t-PA/PAI-1 and 6-Keto-PGF1α/TXB2 could prevent the development of hyperhomocysteinemia.

Objective To observe the effects of aerobic exercise on the plasma content of homocysteine (Hcy),total nitric oxide synthase(T-NOS) and nitric oxide (NO) in rats fed a high methionine diet. Methods A total of 24 male Wistar rats were randomly divided into a normal diet(CR)group,a high methionine diet(MR) group and a high methionine diet plus swimming group(T+MR),and treated accordingly for 8 weeks.At the end of the treatment,all the animals were tested for plasma T-NOS,NO and homocysteine levels.Results Plasma homo-cysteine doubled,whereas T-NOS and NO levels in the MR group decreased significantly as compared with the CR group,suggesting that hyperhomocysteinemia was induced by the high methionine diet.Plasma homocysteine content decreased significantly in the T+CR group,but plasma T-NOS and NO increased significantly compared with the MR group.These indicators were not significantly different for the T+MR group from those of the CR group. Conclu-sion Adequate aerobic exercise can decrease the plasma level of homocysteine in rats fed a high methionine diet,and increase the plasma level of T-NOS and NO,helping prevent the development of hyperhomocysteinemia.

Objective To evaluate the effect of SYNTAX score in the emergency revascularization strategy selection in ST‐segment elevation myocardial infarction patients with multi‐vessel disease(MVD) and to analyze the patient′s prognosis and influen‐cing factors .Methods A total of 144 patients with STEMI complicating MVD verified by coronary arterial angiography in the Xin‐qiao Hospital of Third Military Medical University from August 2010 to March 2012 were collected and divided into the once com‐plete revascularization group (CR group) ,staged complete revascularization group (SR group) and incomplete revascularization group (IR group) according to different emergency PCI strategies .The basic clinical conditions and coronary arterial SYNTAX score were recorded .The occurrence rate of major adverse cardiovascular events (MACCE) during 12 months follow up period was performed the statistics .The results of coronary arterial angiography were collected .The effect of different strategy on prognosis and the risk factors affecting prognosis were analyzed .Results There was no statistically significant difference in the all‐cause mor‐tality between the IR group with the CR and SR groups (P>0 .05) ,while the cerebrovascular event ,repeat revascularization during hospitalization ,AMI and total MACCE occurrence rate had statistical difference between the IR group with the CR and SR groups (P<0 .05) .The main influence factors of MACCE were complicating diabetes mellitus ,type C lesions ,SYNTAX score ,D‐B time , myocardial infarction area ,TIMI score in PCI ,no reflow existence and cardiac shock (P<0 .05) .According to the SYNTAX score , the occurrence rate of MACCE in mild or severe lesions had no statistical difference among 3 groups(P> 0 .05) ,while which in moderate lesion had statistical difference among 3 groups(P<0 .05) ,the CR group had the highest occurrence rate of MACCE . Conclusion For the patients with STEMI complicating MVD ,the SYNTAX score can be used as the evidence for selecting reperfu‐sion strategies and applied in emergency PCI .

Objective The aim of this study is to explore the significance of fitting hearing aids before cochle_ar implantation by studying the postoperative impact on neural response telemetry (NRT ) threshold ,the subjective threshold(T -level) and maximal comfort level(C-level) values .Methods A total of 45 patients with cochlear im_plants were divided into 3 groups:patients in group HA1(n=17)had worn hearing aids preoperatively more than 6 months and the age of starting wearing hearing aids was yonger than 3 .5 years old ,patients in group HA2(n=8) had worn hearing aids preoperatively more than 6 months and the starting age was older than 3 .5 years old ,patients in group NHA(n=20 )never wore hearing aids before ACI .The postoperative electrode impedance ,NRT value and T -level and C -level were evaluated at 3rd ,6th ,and 12th months ,respectively .ResuIts Electrode impedances were 10 .9 ± 2 .1 kΩ in NHA group ,10 .5 ± 2 .3 kΩ in HA1 group ,10 .7 ± 2 .7 kΩ in HA2 group ,respectively ,and there were no statistically significant differences .Significant differences were found between group HA1 and group NHA ,group HA1 and group HA2 in terms of NRT threshold value ,T -level or C-level(P<0 .05) .There were no statistically significant differences between group HA2 and group NHA with respect to NRT threshold values ,T-level as well as C-level(P>0 .05) .ConcIusion The results of this study showed that wearing hearing aids for a longer time before CI was beneficial for hearing rehabilitation postoperatively .

Adult Stem Cells ; Myocytes, Cardiac ; cytology

Objective To investigate the single nucleotide polymorphisms ( SNP) of IL-13G+2044A and IL-13A-1512C and to analyze their relationships with the occurrence of bronchial asthma in chil-dren in Guiyang city. Methods Sequenom MassArray platform, a newly developed genotyping assay, was used to detect the G+2044A locus and the A-1512C locus in 153 subjects with asthma and 103 healthy sub-jects. Then the results were statistically analyzed with chi-square test and t-test. Results Three genotypes of IL-13G+2044A locus including GG, GA and AA were detected in the subjects with or without asthma. There were significant differences in the genotype distribution between the subjects with or without asthma (χ2 =7. 691, P<0. 05). The subjects carrying the variant allele A at IL-13G+2044A locus were more likely to have asthma than those not harboring the variant allele A. There were significant differences in allele fre-quency between the subjects with or without asthma (χ2 = 7. 458, P<0. 01). Three genotypes of IL-13A-1512C locus including AA, AC and CC were detected in the subjects from both of the two groups. There were significant differences in the genotype distribution between the two groups (χ2 = 12. 906, P<0. 01). The variant allele C at IL-13A-1512C locus was associated with lower risk for asthma. Significant differences in allele frequency were observed the two groups (χ2 =10. 407, P<0. 01). Conclusion Both of the alleles at IL-13G+2044A and IL-13A-1512C loci were associated with asthma. The variant allele A at IL-13G+2044A locus might be the gene predisposing children to asthma in Guiyang city. Children carrying the variant allele C at IL-13A-1512C locus showed a lower risk for asthma in Guiyang city.

Objective To evaluate the role of histone methylation in the spinal cord in maintenance of neuropathic pain (NP) in rats.Methods Sixty healthy male Wistar rats, weighing 200-250 g, aged 6-8 weeks, were randomly divided into 5 groups (n =12 each) using a random number table: control group (C group), sham operation group (S group), NP group, chaetocin group (CH group), and dimethyl sulfoxide (DMSO) group.NP was induced by chronic constriction injury (CCI) to the sciatic nerve in NP, CH and DMSO groups.The sciatic nerve was only exposed in group S.H3K9 methylation methyltransferase inhibitor chaetocin 50 μg was injected intrathecally in group CH, and the equal volume of DMSO was given instead in group DMSO for 4 consecutive days, starting from 2nd day after CCI.The mechanical paw withdrawal threshold (MWT) and thermal paw withdrawal latency (TWL) were measured before CCI (T0),before administration on 2nd day after CCI (T1), and at 5, 7, 9 and 14 days after CCI (T2-5).Six rats in each group were sacrificed after measurement of pain threshold at T3 and T5, and the lumbar segment of the spinal cord was removed for detection of the methylated H3K9 expression by immunohistochemistry.Results Compared with C group, no significant change was found in the MWT, TWL and methylated H3K9 expressionat each time point in S group (P＞0.05) , the MWT was significantly decreased, and the TWL was shortened at T1-5, and the methylated H3K9 expression was up-regulated at T3 and T5 in NP and DMSO groups, and the MWT was significantly decreased, and the TWL was shortened at T1 in CH group (P＜ 0.05).Compared with NP group, the MWT was significantly increased, and the TWL was prolonged at T2-5, and the methylated H3K9 expression was down-regulated at T3 and T5 in CH group (P＜0.05) , and no significant change was found in the MWT, TWL and methylated H3K9 expression at each time point in DMSO group (P＞0.05).Conclusion Histone methylation in the spinal cord is involved in the maintenance of NP in rats.

Kawasaki disease (KD) is an acute systemic vasculitis that primarily affects young children aged from 6 months to 4 years.KD may lead to coronary artery abnormalities in 25％-30％ of the children.The timely use of high dose intravenous immunoglobulin (IVIG) can significantly reduce the incidence of coronary artery aneurysms.Most clinicians use IVIG combined with aspirin as conventional therapy.But how to deal with those IVIG-resistant patients is a dilemma.With the deepening of research,many researchers have found that some second-line or third-line drugs such as corticosteroids,tumor necrosis factor-α inhibitors,and calcineurin inhibitors work better,and can effectively reduce the complication rate.The recent advances of the treatment of KD were summarized in this paper.

Nowadays, LAN can't meet the requirements of field communication, and WLAN is playing more and more important role for its strong transmission ability and high mobility. This paper introduces the application of WLAN to filed medicine.