Objective To study the method and therapeutic results of intra-arterial interventional treatment (selective intra-arterial fibrinolysis) in acute cerebral infarction(ACl).Methods A total of 106 patients with ACI are divided into two groups:selective intra-arterial fibrinolytic therapy group(SIAF),intra-venously thrombolysis group(IVF);SIAF is selective entered into the obliterative artery with improved Seldinger's technich,pumped urokinase(UK) in a dose of 500 000 i.u.in NS.60ml within 40 minutes;arteriography is done all the time to know if the obliterative artery is unobstruct- ed,if it remains obstructe,then pumps 300 000 i.u.of UK,as long as it unobstructed,the treatment is finished at once; IVF is injected 1000 000 i.u.of UK in NS.150 mL by intravenous drip within 40 minutes,others like SIAF.Results The artery is disobliteration after thrombolysis,48 patients in SIAF,30 patients in IVF,P

Objective To explore the different effects of patiants with Myocardial infarction(MI)accompany with HF's LSVF and the prognosis between complete reftow and partial reflow.Methods 57 cases with MI accompany with HF were randomly divided into 2 groups:complete reflow group and partial reflow group.The two groups were done low-dose Dobutamine loading experiment seperately before operations.22 cases were treated by complete tallow using PCI,other 35 Patients were dealed with Partial reflow using PCI.To contrast the data of LVSF,WMSI,A/V and LVED between the two groups with UCG before and after PCI.Results The complete reflow group was obviously more arailable than the partial group.Conclusion The complete reflow can improve LVSF of the paticnts with MI accompany with HF.

Objective To investigate the influence of HCV core protein on cell apoptosis, cell cycles and cell telomerase activities of HepG2 cells. Methods A eukaryotic expression plasmid containing HCV C gene was constructed by DNA recombinant technique and the recombinant plasmid was transfected into HepG2. Thereafter, HepG2 cells transfected with recombinant eukaryotic expression plasmid were obtained. The HCV C mRNA and protein in HepG2 cells transfected with recombinant plasmid were verified by RT PCR and indirect immunofluorescence assay. The HepG2 cells were studied as follows: (1) The cell proliferation ratio of three groups cells(HepG2 cells transfected with the recombinant plasmid, HepG2 cells transfected with blank plasmid and HepG2 cells without transfection) was evaluated by MTT assay; the cell cycles were also examined by FACS. (2) The apoptotic ratio of three groups cells were examined by FACS. (3) The cell telomerase activities of all three group cells were examined by TRAP ELISA assay. Results (1) The cell proliferation ratio in the group of HepG2 cells transfected with recombinant plasmid was higher than that of the group of HepG2 cells transfected with blank plasmid or the group of HepG2 cells without transfection; The proportion of phase S in the group of HepG2 transfected with the recombinant plasmid was significantly higher than that of the group of HepG2 without transfection. (2) The apoptotic ratio in the group of HepG2 cells transfected with recombinant plasmid was significantly lower than that of the group of HepG2 cells transfected with blank plasmid or the group of HepG2 cells without transfection. (3) There were no significant differences among the three group cell telomerase activities. Conclusions (1) HCV C protein had the potential role in inhibiting cell apoptosis. (2)HCV C protein could induce HepG2 cells from phase G 0/1 to phase S, and might promote cell proliferation, inhibit cell apoptosis. (3) HCV C protein had no influence on cell telomerase activities of HepG2 cells, thus HCV C protein regulated cell cycle, promoted cell proliferation and inhibited cell apoptosis not by enhancing cell telomerase activities.

Objective To establish a hepatocyte line from human for bioartificial liver support system and hepatocyte transplantation. Methods Hepatocytes isolated from donor liver of a 25 year old brain death male were transfected with pcDNA3.1(-) recombined vector containing the genes encoding Simian Virus 40 large T antigen. Characteristics of the immortalized hepatocyte line were evaluated by morphologic and functional methods. Results One of the hepatocyte clones displayed highly differentiated liver function and immortalized characteristics has been screened after 42 day's selection of 700～300 ?g/ml G418. The immortalized hepatocyte appeared epithelial and displayed morphologic characteristics of liver parenchymal cells. ALB, AST, ALT and LDH secreted from hepatocyte line were detected. Transmission electron microscope examination revealed that immortalized cells contained a round nucleus with nucleoli, moderate numbers of mitochondria, numerous rough endoplasmic reticulums, and some vacuoles. Adjacent cells were joined with junctional complexes. RT PCR and Western blot detection revealed that immortalized hepatocyte could express ALB mRNA and showed immunoreactive of cytochrome P450 2E1. Conclusions The new established hepatocyte line from human retained some specificbiologic functions and morphological cha racteristics of primary cultured hepatocytes.

Objective To observe the specific immune responses in mice induced by co immunization of DNA vaccine of HBcAg and plasmids encoding interleukin 12 and interleukin 18. Methods The mice were divided into following groups: vector alone, DNA vaccine of HBcAg alone, DNA vaccine of HBcAg plus plasmid of interleukin 12, DNA vaccine of HBcAg plus plasmid of interleukin 18, and DNA vaccine of HBcAg plus plasmids of interleukin 12 and interleukin 18. The mice were immunized with above DNA constructs by intramuscular injections. The levels of anti HBc and its isotypes(IgG1,IgG2a) in sera, and the level of IFN ? in supernatant of spleno lymphocyte cultures were measured by ELISA methods. CTL acti vities of spleno lymphocyte were detected with LDH release assay. Results Mice in all groups except for vector alone were sera positive for anti HBc. Comparing with group of DNA vaccine of HBcAg alone, groups of DNA vaccine of HBcAg plus plasmid of interleukin 12, DNA vaccine of HBcAg plus plasmid of interleukin 18, and DNA vaccine of HBcAg plus plasmid of interleukin 12 and interleukin 18 showed much higher end point titers of anti HBc( P

Objective:to collect and summary mental disturbance caused by severe craniocerebral injury Method:to compare 150 cases of mental disturbance caused by severe craniocerebral injury with those (20 cases) with similar injury but without mental disturbance Result:1) The rate of those with mental disturbance in patients with severe craniocerebral injuries was 20 8% The rate of that in all patients of head injury was 5 2% 2) 107 of our sample (71 3%, 107/150) had sub-arachnoid hemorrhage 27 had fracture at the base of skull 3) Compared with those without mental disturbance, the occurrence of mental symptoms closely related to severity of injury 4) Delirium was the early symptom, while memory deficiency and affective disorder were common on the later stages Conclusion:Patients with brain contusion with subarachnoid hemorrhage are more likely having mental disturbance

Objective To investigate the effect of tacrolimus on Langerhans cell migration in order to understand the therapeutic mechanism of tacrolimus. Methods C57BL/6 mouse injected with different doses of tacrolimus was stimulated by FITC at ear back. After twelve hours, the number of Langerhans cells which took antigen and migrated to lymph nodes was measured by flow cytometry. The number of Langerhans cells which took antigen and resided in epidermis was examined by immunohistochemical staining. Results After injection with tacrolimus, the number of Langerhans cells which took antigen and migrated to lymph nodes was reduced, especially at 12 hours before stimulation by FITC. The reduction was significantly different between the high dose and low dose injection groups. However,the number of Langerhans cells residing in epidermis was significantly higher in tacrolimus injection group than that in the control. Conclusions Tacrolimus, a new kind of immunosuppressive drug,may probably suppress Langerhans cell migration and therefore inhibit immune response in some diseases.

Objective To study the prevalence of intestinal metaplasia (IM) ,dysplasia and carcinoma at the distal esophagus and esophagogastric junctions as well as the relationship between SCJ and RE. Methods In 391 patients the main symptoms , RE and its degree by gastro endoscopy, and biopsy specimens from the mucosa just below SCJ were investigated. Results The prevalence of IM in LSBE,SSBE and GEJ is 26. 53% , 33.85% and 34.00% respectively; It increases with age after 40 years old and no difference occurs between' the male and female. ;12 cases of dysplasia were diagnosed ( low -grade 7 cases , medium and high-grade 5 cases) . Sixteen cases of cardia carcinomas and 1 case of esophageal adenocarcinoma were diagnosed; The longer the SCJ moved upward above GEJ, the higher the prevalence and severeness of RE . Conclusions 1. There is no difference about the prevalence of IM among LSBE,SSBE and GEJ; 2. It is important to pay attention to the diagnosis of dysplasia in the distal esophagus and esophagogastric junctions; 3. The prevalence of cardia carcinomas is much higher than that of esophageal adenocarcinoma.

Objective:To study the relation between the costimulating molecules CD40,CD80 on DC and their antigen presentation function in patients with chrnic HBV infection .Methods:The DC from healthy donors and patients were stained with anti human specific mouse monoclonal antibodies conjugated with fluorescein isothiocyanate(FITC) respectively.Mouse McAb used were anti HLA ABC,HLA DR,CD1a,CD14,CD40,CD80.Results:There are no remarkable difference in MHC I?MHC II between two groups;remarkable difference was showed in CD40(P

Objective To observe the expression of vascular endothelia l growth factor (VEGF)and transforming growth factor a 1 (TGF-a 1 )in callus of non-union,bone defect for investigation of the molecular mechanisms of bone defect resulted from non-union,and further explore the co rrelative influences of VEGF and TGF-a 1 in fracture healing.Methods The bone defect resulted from non-union model and the fracture healing model were established in the radius of rabbit.The sp ecimens of callus and soft tissue were collected postoperatively at1,2,4, 6,8and12weeks.The expressions of VEGF and TGF-a 1 were in-vestigated by means of immunohistochemistry(SP method).Results There was no sign of subperiosteal healing except a large segment filled with hemorrhage and granulation tissue in bone defect area.There was only a little amount of granulation tissues transforming into chondrocytes.Some chondrocyt es stopped prolif-erating after reaching hyper-trophic chondrocyte stage but changed into fibrous connective tissues.Most of the granulation tissue finally changed to fibrous connective tissues in the fracture gap.In addition,there w ere a large number blood vessels invading the normal fracture healing site than that in non-union.In im muno-histochemistry observation,there were significa nt decreasing expressions of VEGF and TGF-a 1 in callus of different period of non-union comparing to union(P