Objective To investigate the effect of tacrolimus on Langerhans cell migration in order to understand the therapeutic mechanism of tacrolimus. Methods C57BL/6 mouse injected with different doses of tacrolimus was stimulated by FITC at ear back. After twelve hours, the number of Langerhans cells which took antigen and migrated to lymph nodes was measured by flow cytometry. The number of Langerhans cells which took antigen and resided in epidermis was examined by immunohistochemical staining. Results After injection with tacrolimus, the number of Langerhans cells which took antigen and migrated to lymph nodes was reduced, especially at 12 hours before stimulation by FITC. The reduction was significantly different between the high dose and low dose injection groups. However,the number of Langerhans cells residing in epidermis was significantly higher in tacrolimus injection group than that in the control. Conclusions Tacrolimus, a new kind of immunosuppressive drug,may probably suppress Langerhans cell migration and therefore inhibit immune response in some diseases.

Objective To study the prevalence of intestinal metaplasia (IM) ,dysplasia and carcinoma at the distal esophagus and esophagogastric junctions as well as the relationship between SCJ and RE. Methods In 391 patients the main symptoms , RE and its degree by gastro endoscopy, and biopsy specimens from the mucosa just below SCJ were investigated. Results The prevalence of IM in LSBE,SSBE and GEJ is 26. 53% , 33.85% and 34.00% respectively; It increases with age after 40 years old and no difference occurs between' the male and female. ;12 cases of dysplasia were diagnosed ( low -grade 7 cases , medium and high-grade 5 cases) . Sixteen cases of cardia carcinomas and 1 case of esophageal adenocarcinoma were diagnosed; The longer the SCJ moved upward above GEJ, the higher the prevalence and severeness of RE . Conclusions 1. There is no difference about the prevalence of IM among LSBE,SSBE and GEJ; 2. It is important to pay attention to the diagnosis of dysplasia in the distal esophagus and esophagogastric junctions; 3. The prevalence of cardia carcinomas is much higher than that of esophageal adenocarcinoma.

Objective: To invent the cost efficient home made bovine pericardium buttressing the staple lines in lung volume reduction surgery, and evaluate its feasibility by the means of animal test. Methods: the bovine pericardium of certain type of cattle was chosen, and special management was applied on it, making a sheath like product especially for LVRS. A serial types named A 1, A 2 and A 3 were prepared for the dog model pre clinical experimental test. The smoothness of stapling and dividing, the effectiveness of sealing, and the endurance of pressure were recorded as the index of judgment. Results: Products of all these three types were satisfactory in different degree for utilization, while the type of A 3 was found to suit best. Conclusion: In this study, the home made bovine pericardium buttressing the staple line in LVRS showed satisfactory characteristic in the animal test, and it should have great potential value in the coming clinical use.

Objective To observe the toxicity and adverse reaction of interleukin(IL) 2 gene modified allogenic gastric cancer cell line in far advanced gastric cancer patients. Methods A phase Ⅰ clinical trial was conducted for sixteen far advanced gastric cancer patients with IL 2 gene modified gastric cancer cell line. By gene recombinant technique, human IL 2 cDNA was transfected into human gastric cancer cell line MKN45 via retrovirus based vector.These cells were then inactivated by irradiation (100 Gy) and were cryopreserved for the vaccine. The immunization were administrated subcutaneously at the first, 8 th , 15 th , 29 th and 58 th day. The patients were divided into 4 dosage groups, which the dosage of the vaccine was administrated in each subsequent level. The toxicity and adverse reaction were evaluated by WHO criteria. Results Fifteen of the 16 patients completed the immunization. Side effects of treatment consisted of mild to moderate fever, redness and swelling at the site of injection, which were the most common symptoms. Only one patient abandoned after the third injection due to rapidly progressive disease. Other reactions including allergic shock, bone marrow depression and disturbance of hepatorenal function were not observed during the immunization.In some patients, the serum transferrin, humoral immune parameters such as IgG, IgA, IgM,IL 2 and cellular immune parameters such as CD + 3,CD + 4,CD + 8 had been improved after treatment.Conclusions This trial demonstrates the feasibility, safety and potential therapeutic effects of vaccination of gastric cancer patients with allogenic, gene modified gastric cancer cell line. The dosage of the vaccine recommended for phase Ⅱ clinical trail is 5?10 7 cells per time.

Objective To This paper is to probe the clinical implication of left ventricular function for angiographic no reflow phenomenon after primary percutaneous coronary intervention(PCI). Methods Cilnical and angiographic data of 497 patients after primary PCI were retrospectively analysed between January 1999 and October 2002. Angiographic no reflow phenomenon defined as substantial coronary antergrade flow ≤ TIMI 2 class without mechnical obstruction of embolism, thrombus, dissection and spasm in influence of normal flow after PCI, but, normal flow defined as TIMI 3 class. 58 patients were with angiographic no reflow phenomenon, 60 patients randomized from 439 with coronary antergrade flow TIMIⅢ after primary PCI were normal flow group, with random count table of excel. Results Enzyme value、Killip class、number of Q waves on electrocardiogam and WMS are significance in two groups with the angiographic no reflow phenomenon and with the normal flow( P

Objective: To determine the value of intercostal video mediastinoscopy (VMS) in clinical application. Methods: The clinical data of 126 patients treated with intercostal VMS were summarized retrospectively. Of them, 8 patients with suspected malignant pleural effusion underwent intercostal VMS pleural biopsy and talc poudrage pleurodesis, 118 patients with palmar hyperhidrosis underwent bilateral intercostal VMS sympathectomy. Results: Definitive pathologic diagnosis has been made through VMS pleural biopsy in pleural effusion group, and the efficiency of talc pleurodesis was 100%(8/8). In 118 patients with palmar hyperhidrosis, the symptom of hands sweating disappeared completely after operation. Both hands became dry with an increasing skin temperature of 1 5℃～3 0℃ immediately after operation. No recurrence was recorded during the follow up. No mortality and morbidity were reported in this group. Conclusion: Comparing with VATS, intercostal VMS is easier to operate and less invasive. It is an effective and alternative procedure in the treatment of malignant pleural effusion and palmar hyperhidrosis.

Objective To detect mucin(MUC1,MUC2,MUC5AC) expressions in endoscopic ultrasound guided fine needle aspiration(EUS-FNA) specimens of the pancreatic neoplasms lesions and evaluate their diagnostic value.Methods The expression profile of MUC1,MUC2,MUC5AC from 54 EUS-FNA samples of pancreatic occupying lesions were examined by immunohistochemistry with mono- clonal antibodies.The results were correlated to the eytohistologic and clinical diagnosis of pancreatic cancer.Results Thirty-eight of the 54 patients had pancreatic cancer,6 patients had benign tumor,10 patients had chronic pancreatitis.The sensitivites of cytology and histology in diagnosing pancreatic cancer were 31.6%and 47.4%,respectively.The prevalence of MUC1,MUC2 and MUC5AC expres- sion in EUS-FNA samples of pancreatic cancer were 81.6%(31/38),10.5%(4/38),84.2%(32/38), respectively,and those in benign pancreatic neoplasms were 25%(4/16),31.3%(5/16),43.8%(7/16),respectively.There was statistically significance in MUCland MUCSAC expression between the two groups(P

Objective:to collect and summary mental disturbance caused by severe craniocerebral injury Method:to compare 150 cases of mental disturbance caused by severe craniocerebral injury with those (20 cases) with similar injury but without mental disturbance Result:1) The rate of those with mental disturbance in patients with severe craniocerebral injuries was 20 8% The rate of that in all patients of head injury was 5 2% 2) 107 of our sample (71 3%, 107/150) had sub-arachnoid hemorrhage 27 had fracture at the base of skull 3) Compared with those without mental disturbance, the occurrence of mental symptoms closely related to severity of injury 4) Delirium was the early symptom, while memory deficiency and affective disorder were common on the later stages Conclusion:Patients with brain contusion with subarachnoid hemorrhage are more likely having mental disturbance

Purpose:The aim of this study was to evaluate the changes of NK and T lymphocyte with gastric cancer and before and after operation.Methods:T lympho cyte subsets and NK cell activity of 36 cases of gastric cancer and 30 cases of normal subjects were measured by monoclonal anti human T lymphocyte antibody and MTT assay before and after operation.Results:It was found that before operation the CD3(+),CD4(+) cell and ratio of CD4(+)/CD8(+) were significantly decreased and CD8(+) cell was increased and NK activity decreased with gastric cancer as compared to control group ( P

Purpose:To clarify the different expression patterns of the cyclins and P21 between the normal breast epithelium cells and breast cancer cells of different degrees of malignancy.Methods:Using Western Blot, we detected the protein expression of cyclin D1, E, and p21 in the normal breast epithelium cells AG11132A, ER positive MCF 7 breast cancer cells and ER negative MDA MB 231 breast cancer cells.Results:(1) P21 protein was highly expressed and cyclin E lowly expressed in the normal breast epithelium cells. Compared with the normal breast epithelium cells, Cyclin E were highly expressed in breast cancer cells of MCF 7 and MDA MB 231 cells. Abnormal low molecular weight cyclin E was co expressed with normal cyclin E in both MCF 7 and MDA MB 231 breast cancer cells, but not in normal breast epithelium cells AG11132A. (2) In breast cancer cells, compared with the ER positive MCF 7 cells the ER negative MDA MB 231 cells expressed a high level of cyclin E protein but low p21.Conclusions:(1) There are many forms of differences both quantitive and qualitative between normal cells and cancer cells, as well as between relatively low degree and high degree invasive malignant cancer cells. (2) Cyclin E and P21 may be potential indicators that reflect the malignancy of breast cancer cells, but they should be analyzed as a whole in order to get more accurate prognostic information. [