Objective To explore the effect and mechanism of montelukast sodium on transforming growth factorβ1 (TGF -β1 )expression in children with asthma.Methods 80 children with asthma were divided into 2 groups.The control group was treated with common medicine,and the observation group was treated with monte-lukast sodium.The clinical effect of two groups was evaluated.The peripheral eosinophile granulocyte(EOS)count of children was measured before and after treatment.Double antibody sandwich enzyme -linked immunosorbent assay (DAS -ELISA)was used to detect the serum levels of TGF -β1 and leukotriene E4 (LTE4)before and after treatment.Results The total effective rate of montelukast sodium group was 95.0%,remarkable effect in 28 cases, 10 cases were effective,and 2 cases failed.The total effective rate of the control group was 82.5%,23 cases had remarkable effect,effective in 10 cases,ineffective in 7 cases.The effective rate of the montelukast sodium group was statistically higher than the control group(χ2 =4.200,P <0.05).After treatment,the peripheral eosinophil count of patients of two groups significantly decreased than before treatment (control group:χ2 =3.251,P <0.05;observation group:χ2 =3.214,P <0.05).There was significant difference between the two groups(χ2 =3.254,P <0.05).Before treatment,serum levels of TGF -1 showed no statistical difference between the two groups (P >0.05).After treat-ment,the levels of serum TGF -1,LTE4 of the observation group decreased more remarkably when compared with the control group (TGF -β1:t =2.06,P <0.05;LTE4:t =2.14,P <0.05).Conclusion Montelukast sodium has good clinical effect on childhood asthma.It can inhibit the expression of TGF -1 in childhood asthma,and its mechanism may be related to eosinophils and the level of LTE4.

Diagnosis, Differential ; Humans ; Pneumoconiosis ; diagnosis

Objective To study the clinical value of serum procalcitonin(PCT)in early diagnosis of systemic bacterial infection.Methods 57 suspected bacterial infectious patients in our hospital were selected as observation group,and another 57 healthy subjects were selected as control group.Patients in the observation group were divided into sepsis group (A group,19 cases),local bacterial infection group (B group,19 cases)and bacterial infectious SIRS group (C group,19 cases)according to ACCP/SCCM consensus conference and their clinical manifestations. The serum PCT levels in the 4 groups were detected and compared.Results There was no statistically significant differences in general situation (age,sex,height,weight and body mass index (BMI),etc.)between the two groups (P >0.05).After admission,the PCT level in the observation group was obviously higher than that in the control group,the level of PCT was the highest in group A,and the difference was statistically significant(F =94.88,P <0.001).24h,3d,7d and 10d after admission,there was no statistically significant difference in PCT level in control group(P >0.05);the PCT level in the observation group was gradually decreased,the level of PCT in A group was still higher than that in the other three groups,and the differences were statistically significant(F =62.40,P <0.001;F =65.00,P <0.001;F =58.84,P <0.001;F =63.05,P <0.001).Conclusion Serum PCT has a certain advan-tage in sensitivity and specificity,it can reflect the severity of disease,it also has certain clinical significance in judging the development and prognosis of disease.Therefore,it can be considered as one of the first choice for clinical detection of bacterial infection.

OBJECTIVE:To discuss the role of clinical pharmacists in providing pharmaceutical care in respiratory department.METHODS:The clinical pharmaceutical care provided by clinical pharmacists in respiratory department was analyzed and summarized.RESULTS & CONCLUSION:The pharmaceutical care carried out in respiratory department can help reduce the incidence of medication errors.

OBJECTIVE:To discuss the position and role of hospital in pharmacovigilance.METHODS:The knowledge related to pharmacovigilance was introduced and the status quo and the responsibility assumed by hospital and its stuff in the pharmacovigilance work were analyzed.RESULTS & CONCLUSION:Hospital ought to be one of the main part in the pharmacovigilance,and through training to enhance the knowledge of the doctors,pharmacists and nurses about the pharmacovigilance,so that could build good system for the safety monitoring of drugs,and also could establish rules of pharmacovigilance to reduce ADR,improve rational use of medicine.

Thirty five normal esophageal mucosae were observed with electron microscopy. 11 were taken from resected esophageal specimens and 24 were obtained by using fibroendoscope biopsy forceps from normal areas of esophageal mucosae examined endoscopically.Normal esophageal epithelium is divided into basal, prickle and superficial cell layers. The continuous basement membrane separates lamina propria from basal cells. Occasionally a false rupture appears in the obliguely-cut, basement membrane.Intraepithelial lymphocytes and Langerhans cells were found in the basal cell layers and deep in the prickle cell layers. In the cell cytoplasm of one case, a few rod-shaped Langerhans granules with internal periodic substructure were observed. The Langerhans granule measures 250 nm by 50 nm. The results of our observation showed that Langerhans cells were possibly derived from intraepithetial lymphocytes.In the superficial cell layers, a large number of membrane coating granules were identified in pericytoplasm or in the cytoplasmic processes. A few granules were also found in intracellular spaces.Desmosome fields were seen in prickle cell layers. The fields were in the form of labyrinth made up of numerous desmosomes and microvillus processes. No gap junctions were found in the desmosome fields.Twisted thread-like nuclei and indented bizarre nuclei were found in both the prickle cell layers and superficial cell layers. Capillaries with fenestrated endothelium were embedded in the papillae of lamina propria.

Objective:To establish a ?B2 crystallin gene knockout mice model.Methods: The gene-targeting vector was established by the technique of American inGenious Targeting Laboratory.PCR technique was used to identify the genotype of the model mice and expression of ?B2 crystallin protein was detected by Western blot.Results: Three genotypes were successfully(identified) and expression of ?B2 crystallin protein was not detected in gene knockout mice.Conclusion: The ?B2 crystallin gene knockout mice model has been successfully established.

The activity of interleukin-2 (IL-2) and expression of interleukin-2 receptor (IL-2 R) were measured in children with acute glomerulonephritis (AGN) in order to detemine the mechanism of the disease Our results showed that the activity of IL-2 in peripheral blood lymphocytes (PBL) of children with AGN was lower than that tof the control group (P

Ech1 is the key enzyme of fatty acid β-oxidation metabolism,and mainly locates in peroxisome and mitochondria.Recent studies indicate that abnormal expression of Ech1 is related with liver carcinoma,stomach carcinoma and chronic lymphoid leukemia,but the mechanism is not very clear.Other members of Echfamily are also closely related to many diseases.

Chronic Disease ; Humans ; Male ; Middle Aged ; Mineral Oil ; adverse effects ; Occupational Exposure ; adverse effects ; Pigmentation Disorders ; chemically induced