PFTK1,an important member of cyclin dependent kinase Cdc2 family,is over expressed in many malignancies.PFTK1 involves in multiple processes,such as regulation of cell cycle,tumor invasion and metastasis,and the Wnt signal transduction pathway,which has a significant impact on prognosis of tumor.

Recently, stem cell like side population (SP) cells have been found in many normal organizations and malig?nant tumors, which have the proficiency of differentiation and self-renewal. These cells play an important role in cancer stem cell research, though they occupy a low proportion in total cells. Here, we reviewed the foundation of SP cells, and their rela?tionship with stem cells, and the clinical application in the future.

Objective To investigate the effect of 8-Nitrochyrsin on cell proliferation and cell apoptosis of HL-60 cells in vitro.Methods 8-Nitrochyrsin on proliferation of HL-60 cells was detected by MTT assay;The trypan blue exclusion method was used to count the number of viable and dead cells,then the survival rate and growth curve were achieved;DNA ladder bands were observed by DNA agarose gel electrophoresis;HL-60 cell apoptotic percentage was detected by flow cytometry with PI staining.Results 8-Nitrochrysin inhibited proliferation of HL-60 cells in a concentration-dependent manner;8-Nitrochyrsin possess the inhibitory effect on the proliferation and growth of HL-60 cells;8-Nitrochrysin possess the significantly effect of inducing apoptosis of HL-60 cells.Conclusion 8-Nitrochyrsin possess the inhibitory effect of the proliferation and growth of HL-60 cells and significantly induces apoptosis of HL-60 cells.

Humans ; Infection Control ; Root Canal Therapy

Objective To understand the common complications of PICC in olinical use. Methods Retrospectively summarize the data of PICC used in patients of SICU in 1999. Results In 1999, there were 14 pateints using PICC, among them 12 patients were male and the others were female. The average age was 71 years old. Embolus 4 cases' PICC were pulled out. Phlebitis occured in only one case. There are 2 patients presenting hyperpyrexia pulled the PICC out. But the cultures of PICC were negative. Conclusions The common complications of PICC are embolism,phlebitis and infection.

Objective: To study the chemical constituents in Vitis thunbergii var. taiwaniana specially grown in Taiwan, China. Methods: The compounds were isolated by repeated HP20 macroporous adsorption resin column combined with Sephadex LH-20, ODS, and silica gel column chromatography. Their structures were identified on the basis of extensive spectroscopic data analysis and by comparison of their spectral data reported. Results: Twelve compounds were identified as resveratrol (1), trans-ε-viniferin (2), (7R, 8R)-threo-4, 7, 9, 9'-tetrahydroxy-3, 3'-dimethoxy-8-O-4'-neolignan 7-O-β-D-glucopyranoside (3), (7S, 8R)-urolignoside (4), schizandriside (5), vitisin A (6), vitisin B (7), davidiol A (8), 3, 5, 4'-trihydroxystilbene 4'-O-β-D-glucopyranoside (9), ampelopsin C (10), (7R, 8S)-dihydrodehydrodiconiferyl alcohol 9-O-β-D-glucopyranoside (11), and epicatechin (12). Conclusion: Compounds 3-5 are separated from the plants of Vitis L. for the first time, and compounds 8, 9, 11, and 12 are separated from V. thunbergii var. taiwaniana for the first time.

Humans ; Root Resorption

An evaluation scale of medical services quality was developed on the basis of "patients' experience". The questionnaires were developed by, among others, searching relevant literature, collecting well-established assessment scales, measuring patients' experience and satisfaction, brain-storming, literature analysis. Delphi method was adopted for expert consultation. Scale items were screened and revised. The key indexes were converted. Field surveys were conducted for testing the reliability and validity of the scale. Our modified evaluation scale for measuring medical services quality based on "patients' experience" included 6 dimensions (tangibility, reliability, responsiveness, assurance, empathy and continuity), and 50 items. The novel scale based on "patients' experience" may better serve the purpose of assessing medical services quality.

Objective To investigate the feasibility and efficacy of combined preoperative chemora- diation program followed by radical surgery in bulky early or moderate uterine cervical cancer.To determine the incidence and predictive value of rasidual disease in the radical hysterectomy specimens after CCR. Methods Thirty-five patients with uterine cervix carcinoma from January 2001 to September 2003 were treated with preoperative external beam radiotherapy to 3060 cGy in 4 weeks.Patients received concurrent continuous infusion cisplatin(20 mg/m~2)on day 1,8,15,22 of four weeks and 5-Fu(4 g)chemotherapy during the first four days and the last four days of the radiation course.Radical surgery was not performed until 4 weeks after the completion of the preoperative treatment.Results Clinical response was 85.71%,complete response,54.29 %;partial response,31.43 %.The analysis of the surgical specimens showed 19 patients had revealed residual disease and 16 patients had revealed no residual disease,the complete pathological response hed been 45.71%.Three-year survival rate was 87.14 % in 35 patients.Three-year survival rate of residual disease patients(76.32 %)was significantly lower than that of no residual disease(100.0 %)(P=0.0358). Three-year survival of cervical stromal invasion less than 1/2 and invasion extra stromal was 100.0 %, 41.67 % respectively(P=0.0109);three-year survival of lymph-vascular space involvement and no lymph- vascula space involvement was 0,85.29 %,respectively(P=0.0148).Conclusion Combined preoperative chemoradiation program followed by radical surgery in bulky early or moderate cervical cancer could reveal an effective efficacy with a tolerant complication.Residual disease is an independent and strong predictive factor.