Objective To analyze the application of color Doppler ultrasonography in diagnosis of diabetic carotid sclerosis and to study the relation between diabetes and carotid sclerosis. Methods Color Doppler ultrasound findings in 68 hospitalized type 2 diabetes patients ( as an observation group) and in 73 healthy individuals ( as a control group) were compared and analyzed from Feb. 2007 to Feb. 2009. Results The tunica intima and media of carotid were significantly thicker in observation group than in control group ( P

Objective To explore the advantages and disadvantages of the double lumen endotracheal intubation and single -lumen endo-tracheal intubation and continuous carbon dioxide insufflation in thoracoscopic esophagectomy .Methods The clinical data of 90 patients in our department of thoracic surgery after thoracoscopic esophagectomy from January 2014 to April 2015 were analyzed .All patients were divid-ed into single-lumen endotracheal intubation (group A)and double lumen endotracheal intubation group (group B).The endotracheal intuba-tion time,operation time,incidence of pulmonary infection,intraoperative and postoperative PaO2,PaCO2,incidence of anastomotic fistula, hospitalization expenses ,length of hospital stay and the incidence of postoperative chylothorax between two groups were compared .Results The difference in intraoperative PaO2,PaCO2,incidence of pulmonary infection,endotracheal intubation time,operation time,hospitalization days and the hospitalization cost between two groups were statistical significance .The difference of the rest index between two groups were no statistical significance.Conclusion Group A has certain advantages in perioperative management ,hospitalization cost and so on,but has disadvantages in perioperative hypoxemia and carbon dioxide retention and acid -base balance disorders .

Objective:To study a set of system for supplying oxygen monitoring and control in networking. Methods: Oxygen monitoring devices, which are laid up the entrance of clinical department, include signal monitoring and control. Electromechanical actuator consists of oxygen valve and oxygen solenoid valve;Sensor group consists of a pressure sensor, an oxygen concentration sensor and a gas flow sensor;The design circuit includes signal acquisition and data processing, communication and the master circuit board, oxygen dedicated solenoid valve control driver circuit board. Results:The center monitoring technology for oxygen supplying in hospital can monitor oxygen pressure, concentration and flow of all clinical departments. Meanwhile, the monitoring center can be timely warned when oxygen pressure and concentration are lower than normal value. If firing appeared in ward, oxygen supplying can be closed by remote automatic or manual oxygen monitoring control device. Conclusion:This monitoring system can ensure quality and security of oxygen supply, and achieve remote monitoring and alarm of oxygen supply, and improve the level of oxygen supply management.

Recently,with the development of the medical technique includes surgery,radiotherapy,chemotherapy and molecular targeted therapy,multi-subject combined modality therapy for esophageal carei noma has been become a tendency instead of surgery alone.Any single treatment is hard to improve the curative effect of treatment for esophageal carcinoma.Systemic individual comprehensive therapy for the different patients with esophageal carcinoma is likely to reduce recurrence rate,improve survival rates as well as to improve the quality of life.

Aerobic glycolysis,which is also termed as Warburg effect,is one of the most common and profound biochemical phenotypes of tumor ceils.Several oncogenes and tumor suppressors genes have been implicated in aerobic glycolysis.MicroRNAs can control this metabolic switch by regulating oncogenes and tumor suppressors genes in a post-transcriptional way.A deeper understanding of the mechanisms and pathways by which miRNAs regulate the aerobic glycolysis will hopefully lead to a new therapeutic strategy for malignant cancer.

About the malignant tumor in China,the hepatocellular carcinoma mortality is second only to lung cancer and serious threat to the life and health of the masses.Furthermore,because most patients has been in advanced cancer during medical treatment,so had lost the chance of one-stage surgical resection.However,the sensitivity of hepatocellular carcinoma to chemotherapy,radiotherapy and other treatments are poor.Transcatheter arterial chemoembolization is the main method of the treatment for patients those have lost the chance of operation,though the clinical effect is significant,the inadequate is also presence,such as tumor necrosis,incompletely clear,residual tumor nidus and the damage of the immune function after operation.Recombinant adenovirus p53 gene can validly infect tumor cells,transcription and expression of p53 protein,it also regulate the expression of related genes inhibiting tumor cell growth and induce cell apoptosis directly or indirectly.It is increasingly highly attention that recombincanting rAd-p53 with transcatheter arterial chemoembolization(TACE) to treat hepatocellular carcinoma.In order to evaluate the clinical value and promising future,we will make a brief summary for the research progress in this area in recent years.

Objective: The purpose of this study is to investigate the mechanism for the TRAIL (TNFrelated apoptosis-inducing ligand) expression in NK (natural killer) cells stimulated by NDV (Newcastle disease virus). Methods: Pure NK cells were isolated by using MACS (magnetic activated cell sorting). The cytotoxicity of NK cells stimulated by NDV was detected by LDH releasing assay. TRAIL transcription levels in NK cells stimulated by NDV were detected through RFQ-PCR (real-time fluorogenic quantitative-PCR). TRAIL expression on NK cell membrane stimulated with NDV for 16 h was analyzed by FCM (flow cytometry). The level of IFN (interferon) -γ was determined by ELISA after NK cells were stimulated by NDV. TRAIL expression on NK cell membrane was analyzed by FCM after applying the anti-human IFN-γ while NDV was added into NK cell medium. Results: The purity of NK cells detected by FCM was (90.60± 1.15)%. NDV can enhance the cytotoxicity of NK cells after stimulation for 16 h, and the cytotoxicity of NK cells reached (22.28±0.84)% after stimulation with 204.8HU NDV. TRAIL mRNA expression levels were increased after stimulation with various concentrations of NDV for 4 h. TRAIL expression on NK cell membrane was significantly increased after stimulation with NDV for 16 h. IFN-γ level was significantly increased after NK cells were stimulated by NDV, consistent with the concentration of NDV and it was time-dependent. IFN-γ level reached a peak of 796.47±37.87 pg/mL after stimulation with 25.6 HU NDV for 16 h. TRAIL expression was significantly decreased after IFN-γ was neutralized. Conclusion: NDV can enhance the expression of IFN-γ in NK cells, and IFN-γ can up-regulate the TRAIL expression. This is one of the mechanisms for TRAIL expression on NK cells stimulated by NDV. Besides this mechanism, there exists another mechanism for TRAIL expression on NK cells directly stimulated by NDV. Copyright © 2012 by TUMOR.

Adult ; Animal Experimentation ; Female ; Hemorrhagic Fever with Renal Syndrome ; etiology ; Humans ; Male ; Medical Laboratory Personnel ; Middle Aged

Objective To investigate the prevalence of metabolic syndrome (MS) among residents in Shuimogou district of Urumqi city and to compare the differences between the Uygur and Han Populations. Methods Adopting cluster sampling, a survey of diabetes mellitus was conducted in Shuimogou district in June, 2008. Data of total 2420 subjects aged over 18 years with complete data, including background information of each individual, blood pressure, lipid profile, blood glucose and so on, were analyzed in this study. The criterion of the China Diabetes society (CDS) in 2004 was applied for diagnosis. Results The crude prevalence rates of MS were 12.85% in total, 14.91% in male and 7.39% in female, respectively. The age-standardized prevalence rates of MS were 9.93% in total, 13.58% in male and 6.11% in female. The prevalence rates of MS in male was higher than that in female (P0.05). The prevalence rates of components of MS were 43.31% for obesity, 30.08% for hypertension, 25.79% for hypertriglyceridemia and 16.28 % for hyperglycosemia. Among the four factors above, there were about 65% subjects who had at lest one abnormal factor according to the CDS criterion. Conclusion There were no differences in the prevalence rates of MS between the Uygur and Han populations. However, the prevalence rate of MS in male was higher than that in female. More than half subjects had at least one abnormal metabolic factor.

Basic fibroblast growth factor(bFGF) is one of the most important factors for wounds healing. BFGF promotes healing of bone fracture by regulating cell proliferation and differentiation of bone tissues, increasing local bone density, and accelerating local angiogenesis. With the progression of bFGF research, more and more attention will be paid to bone repair function of bFGF in bone tissue engineering.