Objective To study the effect of atorvastatin on the levels of high sensitive C-reactive protein (hs-CRP)in coronary heart disease(CHD)patients with different Gensini score.Methods 120 cases of CHD from March 201 1 to September 2015 in our hospital were selected as the research subjects.According to the different Gensini scores,they were divided into group A (Gensini score <40 ),group B (Gensini score 40 -60 ),group C (Gensini score 60 -80 ),group D (Gensini score >80 ),with 30 patients in each group.All patients were given atorvastatin treatment,the therapeutic effect of the four groups,the total cholesterol(TC),three glycerol(TG),high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL -C)and hs -CRP levels after treatment were compared.Results After treatment,the lower the Gensini score,the better curative effect,four groups had statistically significant difference in the therapeutic effects(P<0.05),and the total effective rates of group A,B, C and D were 96.7%,86.7%,76.6%,56.7%.After treatment,the TG,TC,HDL-C,LDL-C in four groups were improved,A group improved significantly better than the B group,B group improved better than those of C group,C group improved better than those of D group,the differences were statistically significant(F=6.253,6.258,5.856, 6.259,all P<0.05).After treatment,the levels of hs-CRP in group A,B,C and D were (8.2 ±3.9)mg/L,(11.9 ± 6.9)mg/L,(19.2 ±9.3)mg/L,(24.5 ±11.6)mg/L,the hs-CRP levels of four groups were lower,hs-CRP level after treatment in group A was lower than that of B,C,D groups;and hs-CRP level after treatment in group B was lower than that of group C,D;hs-CRP level of C group after treatment was lower than that of group D.The differ-ences were statistically significant(t=12.651,9.185,4.982,3.030,all P<0.05).Conclusion The level of serum hs-CRP in patients with CHD is higher,and the level of hs-CRP is positively correlated with the Gensini score.

Objective:To explore the effects and problems of on-the-spot surveys to provide reference for promoting the investigation,analysis and evaluation of adverse drug reactions/events.Method:By the retrospective case study, 27 ADR/ADE death reports and their corresponding on-the-spot surveys were analyzed in Jiangsu province in 2008.Result: The death occurred more in the elderly,the ADR drags were more seen in the use of antibiotics,and ADRs occurred highly in the venous injection,and the the completion rate of the scene survey was 44.44%.Conclusion:The on-the-spot survey needs good cooperations from various departments,and the management of drugs should be strengthened.

OBJECTIVE:To establish an UV method for the dermination of main components in rilmazafone hydrochloride tablets. METHODS: The content of rilmazafone was determined by UV spectrophotometry in accordance with Chinese Pharmacopeia (2005 edition). The detective wavelength was set at 254 nm. RESULTS: The linear range of rilmazafone was 2.5～30 ?g?mL-1 (r=0.999 9) and the average recovery was 99.41% (RSD=0.59%,n=10). CONCLUSION: The method is simple, accurate and applicable for the determination of rilmazafone in this preparations.

OBJECTIVETo study the effect of nadroparin in the migration of breast cancer cells MDA-MB-231 and its action mechanism.

METHODThe MTT test was adopted to observe the effect of different concentrations of naringenin on the growth capacity of breast cancer cells MDA-MB-231. Wound healing and transwell experiment analysis were conducted to detect the effect of naringenin on the migration of breast cancer cells MDA-MB-231. Western blotting was adopted to investigate the effect of naringenin on protein expressions of MDA-MB-231 cell Integrin β3, β1 and matrix metalloproteinase MMP-2 and MMP-9. The computer virtual docking technique was used to evaluate the combining capacity of naringenin and Integrin β3 in vitro.

RESULTNaringenin inhibited the migration of MDA-MB-231 cells in a dose-dependent manner. In wound healing and transwell experiments, with the increase in the concentration of naringenin, the number of migrant MDA-MB-231 cells and the invasion capacity of breast cancer cells decreased. Naringenin could inhibit the protein expression of Integrin β3 in a dose-dependent manner, but with unobvious effect on expression of Integrin β1. Besides, naringenin could significantly inhibit the protein expressions of MMP-2 and MMP-9. The results of the computer virtual docking showed a negative value in the combining capacity between naringenin and Integrin β3, indicating the high affinity between them.

CONCLUSIONNaringenin can inhibit the growth capacity of breast cancer cells MDA-MB-231 and block the migration and invasion of breast cancer cells MDA-MB-231. Its mechanism is to down-regulate MMP-2 and MMP-9 expressions after combining with Integrin β3.

Breast Neoplasms ; drug therapy ; genetics ; metabolism ; pathology ; Cell Line, Tumor ; Cell Movement ; drug effects ; Drugs, Chinese Herbal ; pharmacology ; Female ; Flavanones ; pharmacology ; Gene Expression Regulation, Neoplastic ; drug effects ; Humans ; Integrins ; genetics ; metabolism ; Matrix Metalloproteinase 2 ; genetics ; metabolism ; Matrix Metalloproteinase 9 ; genetics ; metabolism ; Neoplasm Invasiveness

Pim-3,a new member of the the proto-oncogene pim family which expresses serine and threonine kinase activity,shows a high similar to pim-1 and pim-2 at the function,structure and sequence.Pim-3 plays an important regulative role in the cell cycle transform,cell apoptosis and occurrence of the malignant tumors by phosphorylating numerous specific substrates.

Objective To explore the features of nerve electrophysiology in Miller-Fisher syndrome (MFS) in children.Methods Thirty-four children with MFS were selected.The examinations of routine motor sensory nerve conduction,H reflexes of soleus muscle and F wave of median and tibial nerevs were performed.At the same time,33 healthy children of the same age were selected as control group and 30 children with typical Guillain-Barre syndrome (GBS) selected as GBS group,which were compared with children with MFS.Results In children with MFS,routine motor nerve conduction velocity and amplitude,and sensory nerve conduction velocity were in normal range.Twelve cases (35.29％) showed a decrease in sensory nerve amplitude potential (SNAP),5 cases (14.71％) showed sural sparing phenomena (the decline degrees of SNAP in median or ulnar nerve greater than in sural nerve),22 cases (64.71％) showed abnormality of H reflex,and 12 cases (35.29％) showed the decline of SNAE Compared with control group,there was no difference in sensory nerve conduction velocity and SNAP in sural nerve in children with MFS (P＜0.05);While there were significant decreases in SNAP in both median nerve and ulnar nerve (P＜0.05).Compared with GBS group,children with MFS had significantly higher sensory conduction amplitude and faster velocity (P＜0.01).Conclusion A decrease of terminal sensory nerve conduction amplitude could be found in children with MFS,but,the SNAP had limited impact on it.H reflex abnormality is the most common electrophysiologic abnormalities in MFS.

Individual dose monitoring and registry for radiation workers plays a significant role in ionizing radiation protection and occupational health management. Since the nationwide implementation of monitoring from the 1980s, individual dose monitoring for radiation workers in China has been going on nearly 30 years, which is of significance in radiation protection optimization, and occupational health and protection for radiation workers. In order to improve individual dose monitoring and registry, to prevent and control occupational radiation diseases, and to help build "Healthy China" program, this paper presents the evolution of individual dose monitoring and registry in China, discusses the key points and difficulties faced, and proposes suggestions.

China ; epidemiology ; Endemic Diseases ; prevention & control ; statistics & numerical data ; Humans ; Public Health Practice

Objective To study the effect of dehydroepiandrosterone (DHEA) on the immune system by measuring the apoptosis and expression of Fas/FasL mRNA of thymocytes. Methods The cell viability of thymocytes was determined by MTT assay. Thymocyte apoptosis was detected by agrese gel electrophoresis. The expression of Fas and Fas-L was analyzed by a semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) method. Results DHEA decreased the cell viability of thymocytes and induced thymocytes apoptosis and increased the levels of mRNA of Fas/FasL. Conclusion DHEA can induce apoptosis of thymocytes through Fas/Fas-L pathway. It suggests that DHEA may play a role in certain autoimmune diseases related to apoptosis disturbance.

In recent years , respiratory tract infectious diseases and public health events caused by human adenovirus type 7(HADV-7) had been rising.Especially in Asia, the outbreaks of acute respiratory illness caused by HADV-7, accounting for more than 60%in the world, had brought great influence to the public health.In order to prevent the occurrence of HADV-7 epidemic and improve the diagnostic performance of laboratory , the research progress of HADV biological features and typing , HADV-7 epidemiological characteristics , clinical and differential diagnosis and laboratory examination methodologies were reviewed in this paper.