Objective:To evaluate the effect of decalcified freeze-dried bone allograft(DFDBA) and DFDBA with platelet-rich plasma(PRP) in the treatment of periodontal infrabony defects.Methods: Fifteen periodontal infrabony defects(30 sites) in 12 patients with periodontitis(9 patients with chronic periodontitis and 3 patients with aggressive periodontitis) were selected.Three months after initial therapy,they were assigned to either the DFDBA group(10 defects with 20 sites) or the DFDBA with PRP group(5 defects with 10 sites).The patients were evaluated for plaque index(PI),bleeding index(BI),probing depths(PD) and clinical attachment loss(CAL) before the treatment and at 6 months after periodontal bone graft surgery.Results: The PI,BI,PD and CAL in the DFDBA group were 1.7,2.7,6.0 mm and 7.0 mm at baseline;1.5,1.8,3.9 mm and 4.4 mm at the 6 months after periodontal surgery.The PD,CAL and BI in DFDBA with PRP group at baseline were 6.2 mm,7.1 mm and 2.9;3.2 mm,3.6 mm and 1.7 at the 6 months after periodontal surgery.The PI did not change significantly after surgery in both groups.While both groups showed significant improvement(P

Objective To study the clinical significance of plasma adrenomedullin (ADM) and brain natriuretic peptide (BNP) levels in the elderly patients with chronic cor pulmonale. Methods The levels of ADM and BNP in plasma of the elderly patients with chronic cor pulmonale (39 cases in the acute stage and 30 cases in remission stage) were detected by radioimmunoassay. Their correlations with PaO 2,ET-1 and the ratio of right ventricular pre-ejection period to the pulmonary flow acceleration time (RVPEP /AT), which reflected the degree of pulmonary hypertension, were investigated. 27 elderly healthy subjects served as a normal control group. Results The levels of ADM and BNP in the acute and remission stages of cor pulmonale groups were both significantly higher 〔(27.31?6.27)ng/L,(17.22?4.15)ng/L and (66.21?12.79)ng/L,(52.74?8.85)ng/L, respectively〕 than those in the normal control group〔(11.18?3.95)ng/L, (34.52?3.34)ng/L, respectively〕. The levels of ADM and BNP in the acute stage were both significantly higher than those in the remission stage (all P0.05). Conclusions The study suggests that ADM and BNP involve in the pathophysiological process of chronic cor pulmonale in the elderly patients and may play a compensatory role in the disease.

Objective To evaluate the effects of a novel inducible nitric oxide synthase (iNOS) inhibitor (FR260330) in prevention of chronic rejection in a model of rat aortic allograft and to investigate the mechanism of the arterial wall lesion of chronically rejecting solid organ grafts. Methods Male Lewis (LEW, RT1~ l) rats received male ACI (RT1~ a) aorta allografts or LEW aorta isografts. Seven groups (n≥12) were involved in this study. FR260330 and/or tacrolimus were administered orally for 14 or 90 days according to protocol. The degree of intimal proliferation of graft aorta was determined by a computerized image system.Results Both low and high doses of FR260330 or tacrolimus treated grafts showed significantly decreased intima/(intima+media) ratios at day 90 compared with placebo controls. Combined therapy of low-dose of FR260330 with low-dose of tacrolimus produced a significant decrease of intima/(intima+media) ratios with intact endothelium as compared with placebo controls. Anti-?-actin immunohistochemical staining demonstrated that one of the mechanisms of intimal proliferation was related to migration of vascular smooth muscle cells. The intima in iNOS inhibition groups was more smooth than in placebo control group and low-dose FK506 treated groups.Conclusions A selective inhibitor of nitric oxide synthase, FR260330 plays a protective role in chronic aortic allograft rejection in the rat. Combined therapy of low-dose of FR260330 with tacrolimus produces significant protection of immune injury and may serve to improve long-term graft survival and function.

Objective To study the changes of serum high mobility group box 1 protein ( HMGB-1 ) in patients with traumatic acute lung injury (ALI) and determine its correlations with MODS,acute physiology and chronic health evaluation ( APACHE Ⅱ ) scores and whether it can predict the incidence of MODS and mortality.Methods Forty cases of ALI were divided into MODS group (n =13) and nonMODS group (n =27) according to the MODS evaluation standard.The serum HMGB-1 was determined in the control group ( 10 healthy persons) and the MODS and non-MODS groups at days 1,4 and 7.The MODS and APACHE Ⅱ scores were also evaluated.Results Between the groups,the HMGB-1 level at days 1,4 and 7 in non-MODS group was higher than that in the control group,while lower than that in MODS group (P＜0.05).Within the non-MODS group,the expression of HMGB-1 showed a high level at day 4 compared with that at day 1 ( P ＜0.01 ) ; the HMGB-1 showed a low level at day 7 with clinical symptom improvement compared with that at day 4,and was even lower than that at day 1 ( P ＜ 0.01 ).Within the MODS group,the serum HMGB-1 level was significantly increased,and continued for several days; the HMGB-1 was decreased slightly at day 7 in comparison with that at day 4,but was still higher than that at day 1.The difference of MODS and APACHE Ⅱ score was significant,with the dynamic change of HMGB-1 level at days 1,4 and 7 after traumatic ALI.The correlative analysis showed that HMGB-1 expression level was remarkably related with MODS score and APACHE Ⅱ score.Conclusions HMGB-1 shows a high expression in patients with traumatic ALI.As a late mediator of inflammation,HMGB-1 usually increases relatively late and lasts for a long duration.HMGB-I level and concurrency MODS are closely related.Routine test of serum HMGB-1 level and joint assessment of MODS and APACHE Ⅱ score are contributive to the prediction of organ dysfunction after traumatic ALI.

AlM:To discuss the clinical effect and safety of the combined surgeries of phacoemulsification and goniosynechialysis for patients with primary angle -closure glaucoma ( PACG) .METHODS:Eighty-three eyes of 83 patients with PACG and cataract were ranomized into two groups. Group A:39 patients with 39 eyes of angle-closure <180.;Group B:44 patients with 44 eyes angle - closure ≥180.. The changes of visual acuity, anterior chamber depth ( ACD) , anterior chamber angle and lOP after operation were measured. The follow-up period was for 1a.RESULTS: The visual acuity was improved significantly in both group after operation. No significantly difference in ACD, anterior chamber angle after operation in two groups (P>0. 05). The lOP in group A was lower than that in group B 3mo after operation, there was statistical significance (P<0. 05).CONCLUSlON:Phacoemulsification and goniosynechialysis can deepen ACD significantly. This method make the anterior chamber angle open, can reduce lOP and improve the patients’ visual acuity, especially in patients with PACG. lt is a safe and effective approach to patients with PACG and cataract.

Objective To establish the technique of loading trehalose into human platelets including temperature, time and concentration of trehalose loading. Methods Intracellular trehalose concentration versus loading time curves at 37℃ and 16℃, mean plateletee volume(MPV) versus loading time curves, and loading concentration curves were investigated and compared. Then loading time and temperature for high loading efficiency of trehalose into human platelets were ascertained. Intracellular trehalose versus trehalose loading concentration curves were made, and loading efficiency was caculated. Results The trehalose loading efficiency of 4 hours at 37℃ was significantly higher than that at 16℃ (11.6% vs 5.6%). MPV at 16℃ increased 43.2% compared with at 37℃, but had no changes along with the change of loading time and concentration. When loading at 37℃, MPV change was positively related with loading time and concentration, and loading time and loading concentration had a cooperative effect. MPV had no changes in 1～4hours loading when trehalose loading concentration was under 50mM. MPV increased with the increase of loading time and concentration when trehalose loading concentration was above 50mM. When trehalose loading concentration was 45mM, loading efficiency at 37℃ reached 13.86% 4 hours aftr loading. Conclusion The basic parameter of trehalose loading technique of human platelets was loading temperature at 37℃, loading time for 4 hours, and loading concentration of 45mM.

This article was aimed to study the inhibition of cell proliferation and induction of apoptosis by β-el-emene extract from traditional Chinese medicine (TCM) Rhizoma Zedoariae on human hepatocellular carcinoma (HepG2) cells, as well as the effect on expression of apoptosis-related proteins in liver cancer-burdened H22 mice. Human HepG2 cells were cultured in vitro. MTT method was used in the determination of β-elemene on cell prolif-eration. Apoptosis of cells were detected by the Annexin V-FITC/PI double staining method. Through the establish-ment of liver cancer-burdened H22 mice model, the inhibition of cell proliferation and induction of apoptosis-related protein expression by β-elemene on tumor-burdened mice were observed. The results showed that β-elemene inhib-ited HepG2 cell proliferation, which was dose- and time-dependent. Annexin V-FITC/PI method detected early apoptotic cells. Inhibitions of tumor growth on tumor-burdened mice from different groups were different. Inhibition of tumor growth in the high-dose β-elemene group was relatively low, and that of the middle-dose was more obvi-ous. It was concluded that β-elemene can effectively inhibit human HepG2 cells proliferation. The inhibition on pro-liferation of cancer cells was through the inducing of cell apoptosis. It was also related to the upregulation of apopto-sis-related protein Caspase-3 and downregulation of NF-κB P65.

Objective To investigate the effect of gene silencing of Y-box binding protein-1(YB-1) by RNA interference on the proliferation and migration in prostate cancer cells lines PC-3 cells .Methods YB-1 siRNAs(pGenesil-1-YB-1-1 and pGenesil-1-YB-1-2) were synthesized and transfected into cloned into the the PC-3 cells by liposome .The expressions of YB-1 were measure by RT-PCR and Western blotting .The proliferation and migration were respectively detected by MTT and Transwell method . Results ThemRNA and protein expressions of YB-1 were significantly decreased by pGenesil-1-YB-1-1 and pGenesil-1-YB-1-2 (P<0 .05) ,compared with the control group ,the inhibition ratio of mRNA expression was 36 .23% and 39 .42% respectively and the inhibition ratio of protein expression was 41 .56% and 55 .33% respectively .The proliferation and migration were significantly decreased by pGenesil-1-YB-1-1 and pGenesil-1-YB-1-2(P<0 .05) .Conclusion YB-1 gene silencing by RNA interference inhibits the proliferation and migration in prostate cancer cells lines PC-3 cells .

Objective To prepare the preparation of ginkgolic acids gel and to study its antibacterial activity in vitro.Methods The gel was prepared with ginkgolic acids as main component and with Carbopol 940 as base material.The content of ginkgolic acids in the gel was determined by high performance liquid chromatography and the minimum inhibitory concentration (MIC) of the gel against staphylocococus aureus and propionibacteria was measured in vitro by means of liquid-doubling dilution.Results The ginkgolic acids gel has inhibitory effects on staphylocococus aureus and propionibacteria to various extents.And the MIC was 31.756 mg?mL-1 for staphylocococus aureus and 0.992 mg?mL-1 for propionibacteria.Conclusion The preparation of the gel is feasiblye and the the properties of the gel were stable.The ginkgolic acids gel possesses good antibacterial effects on staphylocococus aureus and propionibacteria.

Objective To evaluate the benefit of three dimensional (3D) reconstruction images with rotational digital subtraction technique for the clinical applications Methods Conventional two dimensional digital substraction angiography ( 2D DSA ) was obtained on A P and lateral view. Three dimensional digital subtraction angiography ( 3D DSA ) images were obtained by reconstruction of a rotational acquisition on a C arm (LCV+, GE Medical Systems) spinning at 40 degrees per second 53 cases of cerebral angiographies were performed (32 men and 21 women; the age ranged from 19 to 72 years, mean 46 3 years) Results In this series of 53 cases of cerebral angiographies, 5 cases of arteriovenous malformation were all correctly diagnosed by 3D DSA and 2D DSA . Seven cases were misdiagnosed as intracranial aneurysms at conventional 2D DSA but confirmed to be kinking of the vessel by 3D DSA . 41 cases were confirmed to be intracranial aneurysms Of the 41 cases, 5 cases were diagnosed as normal at 2D DSA but confirmed to be intracranial aneurysms at 3D DSA . The total consistency rate of 3D DSA and 2D DSA for the diagnosis of intracranial aneurysm is 77 4% (41/53) The consistent test shows that there was consistency between the two modalities (chi square test, ? 2=5 267, P