Objective To explore the work content of nurses in menopause multidisciplinary outpatient team so as to summarize the nursing work well and improve the quality of nursing work. MethodsThis study used case study of qualitative research. From June 2018 to August 2019, we selected 10 relevant personnel in Menopause Multidisciplinary Teamwork (MDT) Outpatient Department at Beijing Shijitan Hospital of Capital Medical University to interview MDT outpatient nurses about the work content. Data were collected with the method of personalized depth interview and observation, and data were analyzed with the method of content analysis. ResultsA total of 5 themes were extracted by analyzing data including the MDT outpatient work flow management, comprehensive evaluation and diagnosis by interdisciplinary, menopause knowledge education for patients, formulating and implementing the plan of individualized continuous nursing as well as participating in MDT publicity, promotion and team development. ConclusionsNurses take part in effective flow management, comprehensive health evaluation, enriching menopause knowledge education, formulating and implementing the plan of individualized continuous nursing as well as MDT publicity, teaching and research in MDT outpatient work to make MDT outpatient flow simple and efficient, improve patients' health knowledge, medical compliance and satisfaction.

Objective:To explore effect of Glioma-associated oncogene family zinc finger 1(GLI1)on hypoxia induced trans-formation of NR8383 to M1 phenotype and development of pulmonary hypertension(PH).Methods:Fifteen adult male Wistar rats were randomly divided into control group,hypoxia PH model group and hypoxic PH with GANT61 treatment group,with 5 rats in each group.PH related indexes of rats were detected by small animal ultrasound and right cardiac catheter experiment to determine effect of GLI1 specific inhibitor GANT61 on progression of PH.Pulmonary arterial thickness was measured by HE staining.α-SMA and M1 polarization markers TNF-α and IL-1β expressions were determined by immunohistochemistry.M1 polarization markers CD86 and TNF-α expressions were determined by immunofluorescence.GLI1 expression and NF-κB protein were detected by Western blot.mRNA expressions of iNOS,CD86,TNF-α,IL-1β and IL-12 were detected by qRT-PCR.CHIP-PCR verified that GLI1 regulates NF-κB promoter activity.IL-12 content was detected by ELISA.Rat pulmonary artery smooth muscle cells proliferation was detected by CCK-8.Results:GLI1 inhibitor GANT61 could alleviate symptoms of PH in hypoxic rats(P<0.05).Compared with hypoxic group,inhibition of GLI1 reduced expressions of TNF-α and IL-1β in rat lung tissue(P<0.05).In cell experiments,hypoxia induced M1 polarization of NR8383 by up-regulating GLI1 to activate NF-κB pathway,GLI1 overexpression increased expressions of iNOS,CD86,TNF-α,IL-1β and IL-12 in M1 macrophages(P<0.05).NR8383 culture supernatants could stimulate pulmonary artery smooth muscle cell proliferation(P<0.05)and contribute to development of PH.Conclusion:Hypoxia activates NF-κB pathway by up-regulating GLI1 to induce M1 polarization of macrophages contributes to development of PH.