Objective To explore the relationship of expression and the effect of preoperative radiotherapy of endothelin-1 (ET-1) and pyruvate kinase M-2 (PKM2) in rectal carcinoma.Methods Immunohistochemical method was used to detect the expression of ET-1 and PKM2 proteins of rectal cancer tissues in 96 cases.The expressions of ET-1 and PKM2 were analyzed with the effect of preoperative radiotherapy in rectal cancer tissue.Results The high expression of ET-1 protein was 59 cases (61.46％).The high expression of PKM2 proteins was 54 cases (56.25％).The high expressions of ET-1 and PKM2 protein were worsen the effect of tumor regressive grade (TRG) than lower expressions of those after preoperative radiotherapy of rectal cancer tissue (P ＜ 0.05).The protein expression of ET-1 and PKM2 were positively correlated (P =0.006).Conclusions The high expressed ET-1 and PKM2 proteins in rectal cancer are closely related to preoperative radiotherapy resistance.ET-1 and PKM2 proteins are expected to become new targets of radiotherapy sensitivity and radiotherapy sensitization of rectal cancer.

Objective? To explore the effects of prone position combined with reverse Trendelenburg position on improving hypoxemia and respiratory mechanics of acute respiratory distress syndrome (ARDS) patients. Methods? From January 2017 to December 2017, we selected ARDS inpatients in Intensive Care Unit at Affiliated Hospital of Shaoxing University as subjects by convenience sampling. The total of 20 patients were divided into group A and group B with the method of random number table. Patients of group A adopted prone position for two hours firstly, and then they adopted prone position combined with reverse Trendelenburg position for six hours. Patients of group B adopted prone position combined with reverse Trendelenburg position for two hours firstly, and then they adopted prone position for six hours. During treatment, we assessed the function of pulmonary ventilation and respiratory mechanics of patients. Results? There were statistical differences in different time, different groups and interaction between time and groups among ARDS patients between two groups (P＜ 0.01). Two hours after intervention, the oxygenation index of group B was higher than that of group A with no statistical difference (P＞0.05). One, five and eight hours after intervention, the oxygenation indexes of group A were higher than those of group B with statistical differences (P＜ 0.05). The oxygenation index of group A increased with time, and that of group B increased two hours after intervention and then reduced. There was no statistical difference in the respiratory mechanics of patients between two groups (P＞ 0.05). Conclusions? Prone position combined with reverse Trendelenburg position can improve the oxygenation of ARDS patients.