OBJECTIVETo research the function of endophytes of mistletoe in parasitism process of mistletoe in Pterocarya stenoptera.

METHODEndophytes from eight different parts of the mistletoe were separated by explant culture, and further screened by different CMC plates culture and DNS method to get cellulase high productive strains. The distribution of the endophytic fungus parasitized in mistletoe were prepared and stained to demonstrate by histological section of the intumescentia part of the P. stenoptera.

RESULTThe histological section indicated that aboundent of hyphasma were distributed around the haustorium of the mistletoe. Eighty three strains of endophytic fungus were separated, 38 of them were able to degrade cellulose, 19 strains showed high cellulase activity and 10 of which were separated from the parasitic position.

CONCLUSIONEndophytic fungus of mistletoe can secrete cellulase and assist the haustorium of mistletoe to breakthrough the cell walls as well as intercellular space tissues of the P. stenoptera, thus, the endophytic fungus plays an important role in the parasitism process of mistletoe in P. stenoptera.

Cellulase ; metabolism ; Cellulose ; metabolism ; Fungi ; metabolism ; Juglandaceae ; Symbiosis ; Viscum ; cytology ; microbiology

Modern research showed that components in the dried leaf of Cyclocarya paliurus. had various biological activities. The current quality control research was focused on content determination of polysaccharides and flavonoids, while there were less research on quantitative analysis of terpenes and phenolic acids. In this paper, the contents of 16 components of 3 kinds in C. paliurus leaf were determined by UPLC-QE-Orbitrap-MS. The results were as following: good linear relationship of 16 analytes existed within the studied concentration rages (²>0.996), and RSDs were of <3.0% in the precision test and replicate test, with the average recovery rates 95.20%-104.4%, respectively. The results indicatod that the method is simple and accurate, which can be used for the comprehensive quality evaluation of C. paliurus leaf. The established method was applied to determine the contents of 12 batches of C. paliurus leaf from different areas, and the 16 analvtes contents in the samples could be different from several times to dozens times, which indicated that there might be significant quality difference in C. paliurus leaf from different areas.
Flavonoids ; analysis ; Juglandaceae ; chemistry ; Phytochemicals ; analysis ; Plant Leaves ; chemistry ; Polysaccharides ; analysis

OBJECTIVETo evaluate the molluscicidal activities of three Chinese plants N. indicum Mill, R stenoptera DC, and R. japonicum Houtt, and to clarify the molluscicidal mechanism.

METHODSN-butanol extracts and water extracts of the three plants were obtained. The reactions of EST isozyme, glycogen and total protein of snails to the plant extracts were studied.

RESULTSEST electrophoresis showed that EST was an important antidotal enzyme system and reacted strongly to environment. EST changed greatly during the whole exposure period so that it could be viewed as a pathological index of toxicity. Extracts decreased the glycogen content of the snails' soft tissues greatly, and also the protein content.

CONCLUSIONAll extracts show strong molluscicidal activity. The LD50 value of the water extract of N. indicum Mill is as low as 13.2 mg/L. EST can be viewed as a pathological index of toxicity. The energy metabolism abnormity is the key reason for the molluscicidal activities. The biochemical mechanism needs further research.

Animals ; Electrophoresis, Polyacrylamide Gel ; Esterases ; metabolism ; Glycogen ; metabolism ; Isoenzymes ; metabolism ; Juglandaceae ; chemistry ; toxicity ; Molluscacides ; toxicity ; Nerium ; chemistry ; toxicity ; Plant Extracts ; chemistry ; toxicity ; Rumex ; chemistry ; toxicity ; Snails ; drug effects

OBJECTIVETo investigate the chemical components of the leaves of Cyclocarya paliurus.

METHODThe chemical components were isolated by solvent extraction and column chromatography. The chemical structures were identified on the basis of physic-chemical constant and spectral data.

RESULTSix compounds were isolated and identified as kaempferol(I), quercetin(II), isoquercitrin(III), ellagic acid(IV), daucosterol(V) and cyclocaric acid B(VI).

CONCLUSIONCompounds I, II, III and IV were obtained for the first time from this plant.

Ellagic Acid ; chemistry ; isolation & purification ; Flavonoids ; chemistry ; isolation & purification ; Juglandaceae ; chemistry ; Kaempferols ; Plant Leaves ; chemistry ; Plants, Medicinal ; chemistry ; Quercetin ; analogs & derivatives ; chemistry ; isolation & purification

Child ; Humans ; Juglans ; Moxibustion

A 13-year-old male presented a single, elevated, oval, walnut sized, soft mass on the left retroauricular ares which has increased in size since birth, Histopathologic examination revealed a cyst in the subcutaneus area. Cyst wall is lined by normal epiderrnis and cyst cavity contains hairs and keratinous materials.
Adolescent ; Dermoid Cyst* ; Hair ; Humans ; Juglans ; Male ; Parturition

We report a case of papillary eccrine adenoma occurring in a 39-year-old male who had a walnut sized, firm nodule on the right knee without subjective symptoms. Histopathologic exammination revealed a well circumscribed, non-capsulated dermal nodule consisting of multiple ducts with various sizes. These ducts were composed of double layered epithelial cells in most areas, but in some areas severe intraluminal papillation snd pseudostratification into the lumen were seen and the lumina were filled with a homogenous eosinophilic substance and partially with calcium deposition. Immunohistochemical studies with S-100 antigen and csrcinoembryonic sntigen (CEA) were also performed. Positivity for these sntigens strongly supports the hypothesis that this unusual neoplasm differentiates toward the eccrine secretory coil.
Adenoma* ; Adult ; Calcium ; Eosinophils ; Epithelial Cells ; Humans ; Juglans ; Knee ; Male

The idiopathic calcinosis of the scrotum (Shapiro, 1970) is usually multiple, asymtomatic nodules of the scrotal skin which begins m childhood or early adult life, increase in the size and numbers, and sometimes breakdown to discharge chalky contents. Clinically they are most often misdiagnosed as cysts. The forty eight years old male patient visited our hospital on Oct. 14, 1980, because of multiple asymtomatic scrotal masses which are pin head to walnut sized. There is no family history of calcification, scrotal or otherwise. Histology shows naked amorphous masses in the dermis without any evidence of residual cysts, and is acicular clefts in the masses. Most of the tumors were surgically removed in 5 sessions.
Adult ; Calcinosis* ; Dermis ; Head ; Humans ; Juglans ; Male ; Scrotum* ; Skin

A clinically rare case of recurrent meningioma of the orbit of 30 years old male is reported. The right eye invaded with fibroblastic meningioma was enucleated in association of resection of tumor mass ten years ago. The second surgery of socket reconstruction for the palpebral and conjunctival atrophy was performed eight years later. An experience of orbital exenteration to a complete removal of walnut sized firm and palpable mass recurred in the orbital socket with successful result after the histo-pathological examination was described.
Adult ; Atrophy ; Fibroblasts ; Humans ; Juglans ; Male ; Meningioma* ; Orbit

This study investigated the clinical effectiveness and safety of sealed bleaching compared to conventional in-office bleaching using a randomized clinical trial of split arch design. Ten participants received a chairside bleaching treatment on the upper anterior teeth, and each side was randomly designated as sealed or control side. A mixture of Brite powder (PacDent, Walnut, USA), 3% hydrogen peroxide and carbamide peroxide (KoolWhite, PacDent, Walnut, USA) were used as bleaching agent. The control side was unwrapped and the experimental side was covered with a linear low density polyethylene (LLDPE) wrap for sealed bleaching. The bleaching gel was light activated for 1 hour. The tooth shades were evaluated before treatment, after treatment, and at one week check up by means of a visual shade (VS) assessment using a value oriented shade guide and a computer assisted shade assessment using a spectrophotometer (SP). The data were analyzed by paired t-test. In the control and sealed groups, the visual shade scores after bleaching treatment and at check up showed statistically significant difference from the preoperative shade scores (p < .05). The shade scores of the sealed group were significantly lighter than the control immediately after bleaching and at the check-up appointment (p < 0.05). Compared to prebleaching status, the DeltaE values at post-bleaching condition were 4.35 +/- 1.38 and 5.08 +/- 1.34 for the control and sealed groups, respectively. The DeltaE values at check up were 3.73 +/- 1.95 and 4.38 +/- 2.08 for the control and sealed groups. DeltaE values were greater for the sealed group both after bleaching (p < .05) and at check up (p < .05). In conclusion, both DeltaE and shade score changes were greater for the sealed bleaching group than the conventional bleaching group, effectively demonstrating the improvement of effectiveness through sealing.
Hydrogen Peroxide ; Juglans ; Light ; Peroxides ; Polyethylene ; Tooth ; Urea